[Objective] In order to study the influence of bacteria and accessories of chicken manure compost.[Method] The experiment was conducted to investigate the changes of temperature,the quantification of Escherichia coli ...[Objective] In order to study the influence of bacteria and accessories of chicken manure compost.[Method] The experiment was conducted to investigate the changes of temperature,the quantification of Escherichia coli and the odor during chicken manure compost by adding sawdust,rice husk,yeast and lactic acid bacteria respectively.[Result] The results showed that：（1） The yeast group reached the highest fermentation temperature 67.6 ℃,which was 6.9 ℃ higher than that of lactic acid bacteria group,and the fermentation time of yeast group kept over 55 ℃for 16 days,which was 5 days longer than that of lactic acid bacteria group,both of which were better than that of control group（55.9 ℃,5 days）;（2）The highest fermentation temperature of sawdust group was 2.2 ℃ higher than that of rice husk group,and the fermentation time of sawdust group kept over 55 ℃ was 3 days longer than that of rice husk group;（3） The quantification of E.coli reduced from10^5 to 10^2per gram in both of the yeast group and the lactic acid bacteria group.The odor of the yeast group and the lactic acid bacteria group disappeared in the seventh day and the eighth day respectively.[Conclusion] The results showed that the adding of yeast and sawdust was the best condition for chicken manure composting in this experiment.展开更多
为了探讨滩羊卵母细胞体外成熟过程中促卵泡素(FSH)和促黄体素(LH)的最佳添加量,试验设为4组:对照组、FSH组(10、50、100、200、300μg/m L 5个质量浓度梯度)、LH组(5、10、20、50、100μg/m L 5个梯度)和FSH&LH组(100μg/m L FSH+2...为了探讨滩羊卵母细胞体外成熟过程中促卵泡素(FSH)和促黄体素(LH)的最佳添加量,试验设为4组:对照组、FSH组(10、50、100、200、300μg/m L 5个质量浓度梯度)、LH组(5、10、20、50、100μg/m L 5个梯度)和FSH&LH组(100μg/m L FSH+20μg/m L LH),对滩羊卵母细胞的体外成熟培养进行研究,统计第一极体排出率.结果表明:100μg/m L FSH+20μg/m L LH组卵母细胞成熟率最高,为64.64%,明显高于其他3组,差异显著(P<0.05);FSH组中100μg/m L优于其他4个质量浓度梯度和对照组,差异显著(P<0.05);LH组的20μg/m L的质量浓度优于其他4组和对照组,差异显著(P<0.05).因此,在本试验条件下,100μg/m L FSH+20μg/m L LH是滩羊卵母细胞体外成熟培养的最佳培养添加质量浓度.展开更多
[Objective] This study aimed to investigate the appropriate concentrations of follicle-stimulating hormone（FSH）, luteotropic hormone（LH） and estrodiol（E2） during in vitro maturation of Tan sheep oocytes. [Method...[Objective] This study aimed to investigate the appropriate concentrations of follicle-stimulating hormone（FSH）, luteotropic hormone（LH） and estrodiol（E2） during in vitro maturation of Tan sheep oocytes. [Method] Tan sheep oocytes were divided into five groups for in vitro maturation culture： control group, FSH group（10,50, 100, 200 and 300 μg/ml FSH, respectively）, LH group（5, 10, 20, 50 and 100μg/ml LH, respectively）, E2group（5, 10, 25, 50 and 100 μg/ml E2, respectively）, and FSH ＋ LH group（100 μg/ml FSH ＋ 20 μg/ml LH）. The releasing rate of first polar bodies was analyzed. [Result] The maturation rate of Tan sheep oocytes in 100 μg/ml FSH ＋ 20 μg/ml LH group reached the highest（64.64%）, which was significantly higher than that in other four groups（P〈0.05）; among different FSH concentrations,100 μg/ml FSH was superior to other four concentrations and the control group, exhibiting significant differences（P〈0.05）; among different LH concentrations, 20 μg/ml LH was superior to other four concentrations and the control group, exhibiting significant differences（P〈0.05）; among different E2 concentrations, 50 μg/ml E2 was superior to other four concentrations and the control group, exhibiting significant differences（P〈0.05）. [Conclusion] Under the experimental conditions, 100 μg/ml FSH ＋20 μg/ml LH was the most appropriate hormone combination for in vitro maturation of Tan sheep oocytes.展开更多
基金Supported by Ningxia Science and Technology Research Projects"The Carbon Balance Ecological Chicken Technology of Ningxia Desert Research"(KGZ101104)~~
文摘[Objective] In order to study the influence of bacteria and accessories of chicken manure compost.[Method] The experiment was conducted to investigate the changes of temperature,the quantification of Escherichia coli and the odor during chicken manure compost by adding sawdust,rice husk,yeast and lactic acid bacteria respectively.[Result] The results showed that：（1） The yeast group reached the highest fermentation temperature 67.6 ℃,which was 6.9 ℃ higher than that of lactic acid bacteria group,and the fermentation time of yeast group kept over 55 ℃for 16 days,which was 5 days longer than that of lactic acid bacteria group,both of which were better than that of control group（55.9 ℃,5 days）;（2）The highest fermentation temperature of sawdust group was 2.2 ℃ higher than that of rice husk group,and the fermentation time of sawdust group kept over 55 ℃ was 3 days longer than that of rice husk group;（3） The quantification of E.coli reduced from10^5 to 10^2per gram in both of the yeast group and the lactic acid bacteria group.The odor of the yeast group and the lactic acid bacteria group disappeared in the seventh day and the eighth day respectively.[Conclusion] The results showed that the adding of yeast and sawdust was the best condition for chicken manure composting in this experiment.
文摘为了探讨滩羊卵母细胞体外成熟过程中促卵泡素(FSH)和促黄体素(LH)的最佳添加量,试验设为4组:对照组、FSH组(10、50、100、200、300μg/m L 5个质量浓度梯度)、LH组(5、10、20、50、100μg/m L 5个梯度)和FSH&LH组(100μg/m L FSH+20μg/m L LH),对滩羊卵母细胞的体外成熟培养进行研究,统计第一极体排出率.结果表明:100μg/m L FSH+20μg/m L LH组卵母细胞成熟率最高,为64.64%,明显高于其他3组,差异显著(P<0.05);FSH组中100μg/m L优于其他4个质量浓度梯度和对照组,差异显著(P<0.05);LH组的20μg/m L的质量浓度优于其他4组和对照组,差异显著(P<0.05).因此,在本试验条件下,100μg/m L FSH+20μg/m L LH是滩羊卵母细胞体外成熟培养的最佳培养添加质量浓度.
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ12150)~~
文摘[Objective] This study aimed to investigate the appropriate concentrations of follicle-stimulating hormone（FSH）, luteotropic hormone（LH） and estrodiol（E2） during in vitro maturation of Tan sheep oocytes. [Method] Tan sheep oocytes were divided into five groups for in vitro maturation culture： control group, FSH group（10,50, 100, 200 and 300 μg/ml FSH, respectively）, LH group（5, 10, 20, 50 and 100μg/ml LH, respectively）, E2group（5, 10, 25, 50 and 100 μg/ml E2, respectively）, and FSH ＋ LH group（100 μg/ml FSH ＋ 20 μg/ml LH）. The releasing rate of first polar bodies was analyzed. [Result] The maturation rate of Tan sheep oocytes in 100 μg/ml FSH ＋ 20 μg/ml LH group reached the highest（64.64%）, which was significantly higher than that in other four groups（P〈0.05）; among different FSH concentrations,100 μg/ml FSH was superior to other four concentrations and the control group, exhibiting significant differences（P〈0.05）; among different LH concentrations, 20 μg/ml LH was superior to other four concentrations and the control group, exhibiting significant differences（P〈0.05）; among different E2 concentrations, 50 μg/ml E2 was superior to other four concentrations and the control group, exhibiting significant differences（P〈0.05）. [Conclusion] Under the experimental conditions, 100 μg/ml FSH ＋20 μg/ml LH was the most appropriate hormone combination for in vitro maturation of Tan sheep oocytes.
