目的:探究右美托咪定治疗新型冠状病毒肺炎(COVID-19)的相关靶点及作用机制。方法:采用CNKI、PUBMED等数据库检索右美托咪定的作用靶点,采用GeneCards等数据库,检索COVID-19相关靶点,取交集靶点作为共同靶点;采用Cytoscape3.9.1对共同...目的:探究右美托咪定治疗新型冠状病毒肺炎(COVID-19)的相关靶点及作用机制。方法:采用CNKI、PUBMED等数据库检索右美托咪定的作用靶点,采用GeneCards等数据库,检索COVID-19相关靶点,取交集靶点作为共同靶点;采用Cytoscape3.9.1对共同靶点绘制药物一靶点网络图。采用STRING11.5数据库绘制共同靶点相互作用网络图,并导入Cytoscape3.9.1分析获得关键靶点;采用DAVID数据库对关键靶点进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路和基因本体论(Gene Ontology,GO)富集分析。结果:获得右美托咪定主相关靶点192个,与COVID-19相关靶点7080个,取疾病靶点和药物靶点交集,获得31个右美托咪定治疗COVID-19靶点,分析获得19个关键靶点,包括Janus激酶2(JAK2)丝裂原活化蛋白激酶14(MAPK14)前列腺素内过氧化物合酶2(PTGS2)CC型修饰趋化因子配体2(CCL2)等。KEGG和GO富集分析表明右美托咪定治疗COVID-19涉及的信号通路主要为癌症途径、TNF、IL-17等,涉及的生物过程主要包括信号传导、基因表达的正向调节、对RNA聚合酶Ⅱ启动子的转录正向调节等,主要涉及的细胞成分为细胞质、细胞液、浆膜等;主要涉及的分子功能包括相同的蛋白质结合、蛋白激酶活性、蛋白结合等。结论:右美托咪定治疗COVID-19主要通过与多靶点结合,作用于多条关键通路,发挥抑制细胞炎症因子、调节免疫功能、增强抗病毒能力等作用。展开更多
The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown o...The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown on soil contaminated by arsenic (As), cadmium (Cd) and lead (Pb) were studied. The results showed that the shoot biomass of giant reed was enhanced by 24.8% and 15.0%, while superoxide mutase and catalase activities slightly varied when adding 5.0 mmol/kg CA and 2.5 mol/kg EDTA to soil as compared to the control, respectively. The concentrations of As, Cd and Pb in shoots were remarkably increased by the addition of 2.5 mmol/kg AA and CA, 5.0 mmol/kg EDTA, and 4.0 g/kg sepiolite as compared to the control. The accumulations of As and Cd were also significantly enhanced in the above condition, while the shoot Pb accumulation was noticeably enhanced by amending with 4.0 g/kg sepiolite and 8.0 g/kg phosphogysum, respectively. The results suggested that AA, CA and sepiolite could be used as optimum soil amendments for giant reed remediation system.展开更多
文摘目的:探究右美托咪定治疗新型冠状病毒肺炎(COVID-19)的相关靶点及作用机制。方法:采用CNKI、PUBMED等数据库检索右美托咪定的作用靶点,采用GeneCards等数据库,检索COVID-19相关靶点,取交集靶点作为共同靶点;采用Cytoscape3.9.1对共同靶点绘制药物一靶点网络图。采用STRING11.5数据库绘制共同靶点相互作用网络图,并导入Cytoscape3.9.1分析获得关键靶点;采用DAVID数据库对关键靶点进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路和基因本体论(Gene Ontology,GO)富集分析。结果:获得右美托咪定主相关靶点192个,与COVID-19相关靶点7080个,取疾病靶点和药物靶点交集,获得31个右美托咪定治疗COVID-19靶点,分析获得19个关键靶点,包括Janus激酶2(JAK2)丝裂原活化蛋白激酶14(MAPK14)前列腺素内过氧化物合酶2(PTGS2)CC型修饰趋化因子配体2(CCL2)等。KEGG和GO富集分析表明右美托咪定治疗COVID-19涉及的信号通路主要为癌症途径、TNF、IL-17等,涉及的生物过程主要包括信号传导、基因表达的正向调节、对RNA聚合酶Ⅱ启动子的转录正向调节等,主要涉及的细胞成分为细胞质、细胞液、浆膜等;主要涉及的分子功能包括相同的蛋白质结合、蛋白激酶活性、蛋白结合等。结论:右美托咪定治疗COVID-19主要通过与多靶点结合,作用于多条关键通路,发挥抑制细胞炎症因子、调节免疫功能、增强抗病毒能力等作用。
基金Project (2012BAC09B04) supported by National Key Technology Research and Development Program of the Ministry of Science and Technology of ChinaProject (2010-277-027) supported by Science and Technology Foundation of Environmental Protection in Hunan Province,ChinaProject (2011SK3262) supported by Science and Technology Planning of Hunan Province,China
文摘The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown on soil contaminated by arsenic (As), cadmium (Cd) and lead (Pb) were studied. The results showed that the shoot biomass of giant reed was enhanced by 24.8% and 15.0%, while superoxide mutase and catalase activities slightly varied when adding 5.0 mmol/kg CA and 2.5 mol/kg EDTA to soil as compared to the control, respectively. The concentrations of As, Cd and Pb in shoots were remarkably increased by the addition of 2.5 mmol/kg AA and CA, 5.0 mmol/kg EDTA, and 4.0 g/kg sepiolite as compared to the control. The accumulations of As and Cd were also significantly enhanced in the above condition, while the shoot Pb accumulation was noticeably enhanced by amending with 4.0 g/kg sepiolite and 8.0 g/kg phosphogysum, respectively. The results suggested that AA, CA and sepiolite could be used as optimum soil amendments for giant reed remediation system.