此指南是美国肝病学会(American Association for the Study of Liver Diseases,AASLD)和美国感染病学会(Infections Diseases Society of America,IDSA)最新的关于丙型肝炎的共识意见。作者对指南全文的推荐建议及表格内容进行了翻...此指南是美国肝病学会(American Association for the Study of Liver Diseases,AASLD)和美国感染病学会(Infections Diseases Society of America,IDSA)最新的关于丙型肝炎的共识意见。作者对指南全文的推荐建议及表格内容进行了翻译,供业内同行参考。推荐建议的分级见表1。展开更多
The conventional protein chromatography technique was adopted to purify the antifreeze proteins (AFPs) from the leaves of Ammopiptanthus mongolicus (Maxim.) Cheng f. Two bands on native PAGE gel showed thermal hys...The conventional protein chromatography technique was adopted to purify the antifreeze proteins (AFPs) from the leaves of Ammopiptanthus mongolicus (Maxim.) Cheng f. Two bands on native PAGE gel showed thermal hysteresis activity, one was band B1, whose thermal hysteresis was 0.46 ℃ at 8 g/L, which showed two bands (67 kD, 21 kD) on SDS_PAGE gel; the other was B3, whose thermal hysteresis was 0.45 ℃ at 10 g/L, and it contained only a single protein (39.8 kD). Both B1 and B3 are not glycoproteins, because neither do they interact with Shiff_reagent, nor show ultraviolet characteristics of a typical glycoprotein.展开更多
基金support from the National Science Foundation of China (No.51971249)the Natural Science Foundation of Shandong Province,China (No.ZR2020KE012)the Science and Technology Planning Project of Longkou City,China (No.2021KJJH025).
文摘此指南是美国肝病学会(American Association for the Study of Liver Diseases,AASLD)和美国感染病学会(Infections Diseases Society of America,IDSA)最新的关于丙型肝炎的共识意见。作者对指南全文的推荐建议及表格内容进行了翻译,供业内同行参考。推荐建议的分级见表1。
文摘The conventional protein chromatography technique was adopted to purify the antifreeze proteins (AFPs) from the leaves of Ammopiptanthus mongolicus (Maxim.) Cheng f. Two bands on native PAGE gel showed thermal hysteresis activity, one was band B1, whose thermal hysteresis was 0.46 ℃ at 8 g/L, which showed two bands (67 kD, 21 kD) on SDS_PAGE gel; the other was B3, whose thermal hysteresis was 0.45 ℃ at 10 g/L, and it contained only a single protein (39.8 kD). Both B1 and B3 are not glycoproteins, because neither do they interact with Shiff_reagent, nor show ultraviolet characteristics of a typical glycoprotein.