Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin-catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyz...Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin-catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyzed by COA(cholesterol oxydase , COA.) to determine total cholesterol(TC)and free cholesterol (FC) in human serum by flow injection chemiluminescence method. The sampling frequency was 30 s/h. The linear range for free cholesterol is 5.6×10-7mol/L～3.8×10-5mol/L and the detection limit is 3.0×10-7mol/L. The linear range for total cholesterol is 1.1×10-6mol/L～4.3×10-5mol/L and the detection limit is 1.5×10-7mol/L.展开更多
文摘Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin-catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyzed by COA(cholesterol oxydase , COA.) to determine total cholesterol(TC)and free cholesterol (FC) in human serum by flow injection chemiluminescence method. The sampling frequency was 30 s/h. The linear range for free cholesterol is 5.6×10-7mol/L～3.8×10-5mol/L and the detection limit is 3.0×10-7mol/L. The linear range for total cholesterol is 1.1×10-6mol/L～4.3×10-5mol/L and the detection limit is 1.5×10-7mol/L.
