目的:观察中药熏蒸联合强脉冲光治疗睑板腺功能障碍(meibomian gland dysfunction, MGD)性干眼的临床作用。方法:收集风热证MGD性干眼患者72例144眼,随机分为观察组和对照组。对照组予强脉冲光治疗,观察组在对照组的基础上联合中药熏蒸...目的:观察中药熏蒸联合强脉冲光治疗睑板腺功能障碍(meibomian gland dysfunction, MGD)性干眼的临床作用。方法:收集风热证MGD性干眼患者72例144眼,随机分为观察组和对照组。对照组予强脉冲光治疗,观察组在对照组的基础上联合中药熏蒸治疗。评估治疗前后患者的眼部主观症状评分、睑缘改变评分、睑板腺分泌物排出能力评分、睑板腺分泌物性状评分、角膜荧光素钠染色评分及非接触式泪膜破裂时间(noninvasive breakup time, NIBUT)情况。结果:治疗后两组患者眼部各项评分较治疗前均降低,观察组评分低于对照组,差异均有统计学意义(P P Objective: To observe the clinical effect of Chinese herbal fumigation combined with intense pulsed light in the treatment of dry eye caused by meibomian gland dysfunction (MGD). Methods: A total of 72 patients with MGD-related dry eye with wind-heat syndrome (144 eyes) were collected and randomly divided into an observation group and a control group. The control group was treated with intense pulsed light, and the observation group was treated with Chinese herbal fumigation combined with the control group. The subjective ocular symptom scores, eyelid margin change scores, meibomian gland secretion discharge ability scores, meibomian gland secretion characteristics scores, corneal fluorescein sodium staining scores and noninvasive breakup time (NIBUT) of the patients were evaluated before and after treatment. Results: After treatment, the ocular scores of the two groups of patients were lower than those before treatment, and the scores of the observation group were lower than those of the control group, with significant differences (P P < 0.05). Conclusion: Chinese medicine fumigation combined with intense pulsed light can improve the NIBUT of patients with MGD dry eye, improve ocular symptoms, eyelid margin and meibomian gland secretion characteristics, promote meibum discharge, and reduce corneal fluorescein sodium staining.展开更多
文摘目的:观察白藜芦醇对多囊卵巢综合征(polycystic ovary syndrome,PCOS)模型大鼠卵巢颗粒细胞增殖和凋亡的影响以及线粒体的形态和功能的改变,并探讨其可能的保护机制。方法:32只雌性SD大鼠幼鼠随机分为2组,PCOS模型组(n=24)和模型对照组(n=8)。成功提取PCOS组颗粒细胞并体外培养,然后用不同浓度的白藜芦醇处理颗粒细胞,CCK-8法检测细胞活力的变化;Mito Tracker Deep Red染色观察细胞线粒体的形态变化;JC-1检测线粒体膜电位的变化;ATP检测试剂盒检测ATP的变化;分光光度计检测与凋亡密切相关casppase-3和caspase-9酶活性变化;最后,蛋白免疫印迹法检测颗粒细胞内caspase-3和caspase-9蛋白水平的变化。结果:白藜芦醇处理PCOS模型大鼠卵巢颗粒细胞后,颗粒细胞的增殖活性明显增强(P=0.000);Mito Tracker Deep Read染色结果显示线粒体质量明显增加,形状呈管状或长条状;颗粒细胞内ATP产量和线粒体膜电位也明显增加(P=0.000、P=0.000)。另外,白藜芦醇也降低了caspase-3和caspase-9酶活性和蛋白水平的表达(P=0.000、P=0.000)。结论:白藜芦醇抑制了PCOS模型大鼠卵巢颗粒细胞的凋亡,也促进了细胞的增殖,其机制与白藜芦醇改善颗粒细胞线粒体的形态和功能有关。
文摘目的:观察中药熏蒸联合强脉冲光治疗睑板腺功能障碍(meibomian gland dysfunction, MGD)性干眼的临床作用。方法:收集风热证MGD性干眼患者72例144眼,随机分为观察组和对照组。对照组予强脉冲光治疗,观察组在对照组的基础上联合中药熏蒸治疗。评估治疗前后患者的眼部主观症状评分、睑缘改变评分、睑板腺分泌物排出能力评分、睑板腺分泌物性状评分、角膜荧光素钠染色评分及非接触式泪膜破裂时间(noninvasive breakup time, NIBUT)情况。结果:治疗后两组患者眼部各项评分较治疗前均降低,观察组评分低于对照组,差异均有统计学意义(P P Objective: To observe the clinical effect of Chinese herbal fumigation combined with intense pulsed light in the treatment of dry eye caused by meibomian gland dysfunction (MGD). Methods: A total of 72 patients with MGD-related dry eye with wind-heat syndrome (144 eyes) were collected and randomly divided into an observation group and a control group. The control group was treated with intense pulsed light, and the observation group was treated with Chinese herbal fumigation combined with the control group. The subjective ocular symptom scores, eyelid margin change scores, meibomian gland secretion discharge ability scores, meibomian gland secretion characteristics scores, corneal fluorescein sodium staining scores and noninvasive breakup time (NIBUT) of the patients were evaluated before and after treatment. Results: After treatment, the ocular scores of the two groups of patients were lower than those before treatment, and the scores of the observation group were lower than those of the control group, with significant differences (P P < 0.05). Conclusion: Chinese medicine fumigation combined with intense pulsed light can improve the NIBUT of patients with MGD dry eye, improve ocular symptoms, eyelid margin and meibomian gland secretion characteristics, promote meibum discharge, and reduce corneal fluorescein sodium staining.
