植物提取物是畜禽饲料添加剂的重要组成部分,在畜禽养殖中起到抗氧化、抗炎、抗应激和提高生产性能的作用。目前植物提取物的制备多采用常规的有机溶剂(乙醇、氯仿和乙酸乙酯)提取,有机溶剂的易燃、易挥发和毒性会对提取人员造成伤害,...植物提取物是畜禽饲料添加剂的重要组成部分,在畜禽养殖中起到抗氧化、抗炎、抗应激和提高生产性能的作用。目前植物提取物的制备多采用常规的有机溶剂(乙醇、氯仿和乙酸乙酯)提取,有机溶剂的易燃、易挥发和毒性会对提取人员造成伤害,提取后的废液会造成环境的污染。天然低共熔溶剂(natural deep eutectic solvents, NADESs)是一种以初级代谢产物等天然成分为原料的新型绿色提取溶剂,具有制备简单、低毒或无毒、价廉易得、可生物降解、可回收利用等优点,是未来替代传统有机提取溶剂的新型溶剂,已广泛应用到化工、食品、中药提取等技术领域。文章综述了天然低共熔溶剂在植物提取物中的最新应用进展,旨在为天然低共熔溶剂的推广和应用提供指导。展开更多
[Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper （CD） with FITC-conjugated monoclonal antibodies （FITC-McAb）.[ Metbod] The McAb again...[Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper （CD） with FITC-conjugated monoclonal antibodies （FITC-McAb）.[ Metbod] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. [ Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didn＇t have cross reaction with canine parvovirus （CPV）, canine parainfluenza virus （CPIV）, canine adenovirus （CAV） and rabies virus （RV）. The optimal working concentration was 1：80. The positive rate of clinical suspected samples was 48%. [ Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper.展开更多
Objective The research aimed to provide theoretical basis for studying anti-MDV mechanism of ginsenoside and its derivatives in vitro. Method Effects of ginsenoside and its derivatives on proliferation activity of c...Objective The research aimed to provide theoretical basis for studying anti-MDV mechanism of ginsenoside and its derivatives in vitro. Method Effects of ginsenoside and its derivatives on proliferation activity of chick embryo fibroblast （CEF） in vitro were determined by using neutral red dye absorption method. Result The results showed the proliferation effects of different drugs are not completely same, and are more obvious with low toxic drugs. Detected at different action times, the differences of OD values was biggest at 72 h when compared with normal control group, while there was no significant difference at 24 h. Conclusion Ginsenoside and its derivatives could promote the proliferation of CEF cells in medium as low concentrations, which have time-dependent characteristic.展开更多
文摘植物提取物是畜禽饲料添加剂的重要组成部分,在畜禽养殖中起到抗氧化、抗炎、抗应激和提高生产性能的作用。目前植物提取物的制备多采用常规的有机溶剂(乙醇、氯仿和乙酸乙酯)提取,有机溶剂的易燃、易挥发和毒性会对提取人员造成伤害,提取后的废液会造成环境的污染。天然低共熔溶剂(natural deep eutectic solvents, NADESs)是一种以初级代谢产物等天然成分为原料的新型绿色提取溶剂,具有制备简单、低毒或无毒、价廉易得、可生物降解、可回收利用等优点,是未来替代传统有机提取溶剂的新型溶剂,已广泛应用到化工、食品、中药提取等技术领域。文章综述了天然低共熔溶剂在植物提取物中的最新应用进展,旨在为天然低共熔溶剂的推广和应用提供指导。
基金Supported by Science and Technology Foundation of PLA General Lo-gistics Department(06G138)~~
文摘[Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper （CD） with FITC-conjugated monoclonal antibodies （FITC-McAb）.[ Metbod] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. [ Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didn＇t have cross reaction with canine parvovirus （CPV）, canine parainfluenza virus （CPIV）, canine adenovirus （CAV） and rabies virus （RV）. The optimal working concentration was 1：80. The positive rate of clinical suspected samples was 48%. [ Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper.
基金Supported by the National Natural Science Foundation of China(No.30471272)~~
文摘Objective The research aimed to provide theoretical basis for studying anti-MDV mechanism of ginsenoside and its derivatives in vitro. Method Effects of ginsenoside and its derivatives on proliferation activity of chick embryo fibroblast （CEF） in vitro were determined by using neutral red dye absorption method. Result The results showed the proliferation effects of different drugs are not completely same, and are more obvious with low toxic drugs. Detected at different action times, the differences of OD values was biggest at 72 h when compared with normal control group, while there was no significant difference at 24 h. Conclusion Ginsenoside and its derivatives could promote the proliferation of CEF cells in medium as low concentrations, which have time-dependent characteristic.
