In this paper, a kind of thermally linear polymer, poly N isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. A useful method for deter...In this paper, a kind of thermally linear polymer, poly N isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. A useful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mL and 0 1—10 ng/mL. [WT5HZ]展开更多
建立了分析水杨酸、5-氯代水杨酸、三氯水杨酸等水杨酸类化合物的毛细管区带电泳法 ( CZE)。讨论了电压、缓冲溶液的浓度和 p H值对分析结果的影响。其中缓冲溶液的 p H值对分析结果的影响最为显著。 p H<7.40时 ,三种化合物的出峰...建立了分析水杨酸、5-氯代水杨酸、三氯水杨酸等水杨酸类化合物的毛细管区带电泳法 ( CZE)。讨论了电压、缓冲溶液的浓度和 p H值对分析结果的影响。其中缓冲溶液的 p H值对分析结果的影响最为显著。 p H<7.40时 ,三种化合物的出峰顺序为 :三氯水杨酸、5-氯代水杨酸、水杨酸 ;当 p H>7.40时 ,三种化合物的出峰顺序为 :5-氯代水杨酸、水杨酸、三氯水杨酸。水杨酸、5-氯代水杨酸、三氯水杨酸的检出限分别为 0 .1、0 .2和 0 .1μg/m L。展开更多
文摘In this paper, a kind of thermally linear polymer, poly N isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. A useful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mL and 0 1—10 ng/mL. [WT5HZ]