本文旨在观察普伐他汀对鼠源巨噬细胞性泡沫细胞内胆固醇酯含量的影响,探讨此作用与小凹蛋白-1的关系。采用体外培养的鼠源性巨噬细胞株作为研究对象,加入氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)使其形成泡沫细胞,...本文旨在观察普伐他汀对鼠源巨噬细胞性泡沫细胞内胆固醇酯含量的影响,探讨此作用与小凹蛋白-1的关系。采用体外培养的鼠源性巨噬细胞株作为研究对象,加入氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)使其形成泡沫细胞,运用高效液相色谱测定细胞内胆固醇酯的改变,同时运用Western blot检测细胞中小凹蛋白-1的表达,并观察普伐他汀对细胞内胆固醇酯和小凹蛋白-1影响的量效和时效关系。结果显示:普伐他汀可明显降低泡沫细胞内的胆固醇酯与总胆固醇的比值,且在一定范围内呈剂量依赖性和时间依赖性。在泡沫细胞中加入普伐他汀后能够促进小凹蛋白-1的表达,呈剂量依赖性和时间依赖性。上述结果提示普伐他汀通过降低细胞内胆固醇酯的含量,减轻细胞泡沫化程度。普伐他汀的这一作用可能与促进小凹蛋白-1表达上调有关。展开更多
Caveolae are vesicular invaginations in the plasma membrane,caveolin-1 is the marker protein of caveolae.Recently,much attention has been paid to the functions of caveolae/caveolin-1.Studies show that the disorders of...Caveolae are vesicular invaginations in the plasma membrane,caveolin-1 is the marker protein of caveolae.Recently,much attention has been paid to the functions of caveolae/caveolin-1.Studies show that the disorders of cellular cholesterol homeostasis participate in the development of atherosclerosis.Meanwhile,caveolar/caveolin-1 play a critical role in the regulation of cellular cholesterol transport and homeostasis,cell proliferation and migration,signal transduction and tumor suppression and so on.In this review,we provide an in-depth summary regarding the research development of caveolae/caveolin-1 and cellular cholesterol transport.展开更多
基金This work was supported by the National Natural Science Foundation of China (No. 30470719).
文摘本文旨在观察普伐他汀对鼠源巨噬细胞性泡沫细胞内胆固醇酯含量的影响,探讨此作用与小凹蛋白-1的关系。采用体外培养的鼠源性巨噬细胞株作为研究对象,加入氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)使其形成泡沫细胞,运用高效液相色谱测定细胞内胆固醇酯的改变,同时运用Western blot检测细胞中小凹蛋白-1的表达,并观察普伐他汀对细胞内胆固醇酯和小凹蛋白-1影响的量效和时效关系。结果显示:普伐他汀可明显降低泡沫细胞内的胆固醇酯与总胆固醇的比值,且在一定范围内呈剂量依赖性和时间依赖性。在泡沫细胞中加入普伐他汀后能够促进小凹蛋白-1的表达,呈剂量依赖性和时间依赖性。上述结果提示普伐他汀通过降低细胞内胆固醇酯的含量,减轻细胞泡沫化程度。普伐他汀的这一作用可能与促进小凹蛋白-1表达上调有关。
文摘Caveolae are vesicular invaginations in the plasma membrane,caveolin-1 is the marker protein of caveolae.Recently,much attention has been paid to the functions of caveolae/caveolin-1.Studies show that the disorders of cellular cholesterol homeostasis participate in the development of atherosclerosis.Meanwhile,caveolar/caveolin-1 play a critical role in the regulation of cellular cholesterol transport and homeostasis,cell proliferation and migration,signal transduction and tumor suppression and so on.In this review,we provide an in-depth summary regarding the research development of caveolae/caveolin-1 and cellular cholesterol transport.