Objective To evaluate effects of monoclonal antibody (McAb) against TNFa on E. coli infected mice. Methods 30 mice (10 week old, Kunming ) were divided equally into three groups. The first group, as a control, 200 μl...Objective To evaluate effects of monoclonal antibody (McAb) against TNFa on E. coli infected mice. Methods 30 mice (10 week old, Kunming ) were divided equally into three groups. The first group, as a control, 200 μl saline (N S)/mouse was injected intravenously; Second group (untreated group), only E. coli (10 7 organisms/200 μl N S) were injected intraabdominaly; Third group (treated group), E. coli (10 7 organisms) were injected intra-abdominal and McAb against TNFα 2 mg/kg dissolved in 200 μl N S were injected intravenously. After 24 h, observed the survival rate, compared the serum TNF level in blood and investigated pathology of intestine, lung and liver. Results There was a higher survival rate in treated group, with the serum TNF level lowered significantly, and the untreated mice had severe pathologic changes in viscera. Conclusion Using anti-TNFa McAb was effective in reducing mortality rate in mice after infected with E. coli, but could not prevent the pathologic changes.展开更多
文摘Objective To evaluate effects of monoclonal antibody (McAb) against TNFa on E. coli infected mice. Methods 30 mice (10 week old, Kunming ) were divided equally into three groups. The first group, as a control, 200 μl saline (N S)/mouse was injected intravenously; Second group (untreated group), only E. coli (10 7 organisms/200 μl N S) were injected intraabdominaly; Third group (treated group), E. coli (10 7 organisms) were injected intra-abdominal and McAb against TNFα 2 mg/kg dissolved in 200 μl N S were injected intravenously. After 24 h, observed the survival rate, compared the serum TNF level in blood and investigated pathology of intestine, lung and liver. Results There was a higher survival rate in treated group, with the serum TNF level lowered significantly, and the untreated mice had severe pathologic changes in viscera. Conclusion Using anti-TNFa McAb was effective in reducing mortality rate in mice after infected with E. coli, but could not prevent the pathologic changes.
