[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase（DFR）—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR am...[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase（DFR）—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR amino acid and cDNA sequences released by NCBI,molecular evolution of DFR gene was analyzed with bioinformatic software.[Result]Seventeen plants fell into two major branches,Lamiidae and Liliidae.Most of the plants within a same family cluster assembled together,which basically accords with the morphological development of species.Prediction results based on selection pressure using MEGA,DNAsp and PAML software showed that DFR gene in Lamiidae plants underwent strong purifying selection pressure.[Conclusion]Our results laid basis for analyzing the molecular mechanism of enzymatic characteristics of DFR and of the anthocyanin synthesis pathway.展开更多
基金Supported by the Fundamental Research Funds for the Central Universities(SWJTU09BR208)~~
文摘[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase（DFR）—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR amino acid and cDNA sequences released by NCBI,molecular evolution of DFR gene was analyzed with bioinformatic software.[Result]Seventeen plants fell into two major branches,Lamiidae and Liliidae.Most of the plants within a same family cluster assembled together,which basically accords with the morphological development of species.Prediction results based on selection pressure using MEGA,DNAsp and PAML software showed that DFR gene in Lamiidae plants underwent strong purifying selection pressure.[Conclusion]Our results laid basis for analyzing the molecular mechanism of enzymatic characteristics of DFR and of the anthocyanin synthesis pathway.
