用硫酸铵分级盐析法纯化大菱鲆血清免疫球蛋白IgM,所得产物用Sepharose-4B和DEAE-Sepharose Fast Flow阴离子交换层析进一步纯化,以纯化的大菱鲆IgM免疫新西兰大白兔,获得兔抗大菱鲆IgM抗血清。SDS-PAGE电泳显示大菱鲆IgM重链为76 kD,...用硫酸铵分级盐析法纯化大菱鲆血清免疫球蛋白IgM,所得产物用Sepharose-4B和DEAE-Sepharose Fast Flow阴离子交换层析进一步纯化,以纯化的大菱鲆IgM免疫新西兰大白兔,获得兔抗大菱鲆IgM抗血清。SDS-PAGE电泳显示大菱鲆IgM重链为76 kD,轻链为27 kD;纯化的大菱鲆IgM重链与特异性抗血清具有较好的反应,而轻链与抗血清反应不明显;以制备的兔抗大菱鲆IgM抗血清为二抗建立了大菱鲆血清特异性抗体的间接ELISA检测方法,用该方法检测了鳗弧菌灭活疫苗免疫后大菱鲆产生特异性抗的变化规律,大菱鲆在免疫后第1周就产生了特异性抗体,在3周时达到峰值,该特异性抗体可维持13周以上。展开更多
RpoS protein is a σ factor of RNA polymerase that can control the expression of a group-specific gene, thus playing a vital role in bacteria. In bacteria, RpoS expression is under strict control and is mainly regulat...RpoS protein is a σ factor of RNA polymerase that can control the expression of a group-specific gene, thus playing a vital role in bacteria. In bacteria, RpoS expression is under strict control and is mainly regulated at three levels： transcription level, translation level and post-translational level. Environmental stress enters bacterial cells through signal transduction and leads to a series of variations in microenvironment, thereby causing changes of regulator and controlling its levels based on the direct and indirect interaction between regulator and RpoS protein. In addition, RpoS protein has played special roles in bacteria, therefore the changes of RpoS protein levels will lead to variations in expression levels of a large number of genes, thereby causing variations of bacterial response to different environmental stress and changes of certain characteristics of bacteria, which provides a new strategy for the control of bacterial diseases in the future. This paper reviewed the recent progress on the regulation of RpoS protein expression and its function in several common bacteria. Due to the functional complexity of RpoS protein, there are still a lot of unknown functions to be further identified.展开更多
文摘用硫酸铵分级盐析法纯化大菱鲆血清免疫球蛋白IgM,所得产物用Sepharose-4B和DEAE-Sepharose Fast Flow阴离子交换层析进一步纯化,以纯化的大菱鲆IgM免疫新西兰大白兔,获得兔抗大菱鲆IgM抗血清。SDS-PAGE电泳显示大菱鲆IgM重链为76 kD,轻链为27 kD;纯化的大菱鲆IgM重链与特异性抗血清具有较好的反应,而轻链与抗血清反应不明显;以制备的兔抗大菱鲆IgM抗血清为二抗建立了大菱鲆血清特异性抗体的间接ELISA检测方法,用该方法检测了鳗弧菌灭活疫苗免疫后大菱鲆产生特异性抗的变化规律,大菱鲆在免疫后第1周就产生了特异性抗体,在3周时达到峰值,该特异性抗体可维持13周以上。
基金Supported by Science and Technology Program of Shandong Province (No. 2010GHY10501)National Department Public Benefit Research Fond of China (No. 200909020)~~
文摘RpoS protein is a σ factor of RNA polymerase that can control the expression of a group-specific gene, thus playing a vital role in bacteria. In bacteria, RpoS expression is under strict control and is mainly regulated at three levels： transcription level, translation level and post-translational level. Environmental stress enters bacterial cells through signal transduction and leads to a series of variations in microenvironment, thereby causing changes of regulator and controlling its levels based on the direct and indirect interaction between regulator and RpoS protein. In addition, RpoS protein has played special roles in bacteria, therefore the changes of RpoS protein levels will lead to variations in expression levels of a large number of genes, thereby causing variations of bacterial response to different environmental stress and changes of certain characteristics of bacteria, which provides a new strategy for the control of bacterial diseases in the future. This paper reviewed the recent progress on the regulation of RpoS protein expression and its function in several common bacteria. Due to the functional complexity of RpoS protein, there are still a lot of unknown functions to be further identified.
