Identification and characterization of FpRco1 in regulating vegetative growth and pathogenicity based on T-DNA insertion in Fusarium pseudograminearum

Fusarium pseudograminearum is a devastating pathogen that causes Fusarium crown rot(FCR)in wheat and poses a significant threat to wheat production in terms of grain yield and quality.However,the mechanism by which F.pseudograminearum infects wheat remains unclear.In this study,we aimed to elucidate these mechanisms by constructing a T-DNA insertion mutant library for the highly virulent strain WZ-8A of F.pseudograminearum.By screening this mutant library,we identified nine independent mutants that displayed impaired pathogenesis in barley leaves.Among these mutants,one possessed a disruption in the gene FpRCO1 that is an ortholog of Saccharomyces cerevisiae RCO1,encoding essential component of the Rpd3S histone deacetylase complex in F.pseudograminearum.To further investigate the role of FpRCO1 in F.pseudograminearum,we employed a split-marker approach to knock out FpRCO1 in F.pseudograminearum WZ-8A.FpRCO1 deletion mutants exhibit reduced vegetative growth,conidium production,and virulence in wheat coleoptiles and barley leaves,whereas the complementary strain restores these phenotypes.Moreover,under stress conditions,the FpRCO1 deletion mutants exhibited increased sensitivity to NaCl,sorbitol,and SDS,but possessed reduced sensitivity to H_(2)O_(2)compared to these characteristics in the wild-type strain.RNA-seq analysis revealed that deletion of FpRCO1 affected gene expression(particularly the downregulation of TRI gene expression),thus resulting in significantly reduced deoxynivalenol(DON)production.In summary,our findings highlight the pivotal role of FpRCO1 in regulating vegetative growth and development,asexual reproduction,DON production,and pathogenicity of F.pseudograminearum.This study provides valuable insights into the molecular mechanisms underlying F.pseudograminearum infection in wheat and may pave the way for the development of novel strategies to combat this devastating disease.

Heterogeneously elevated branched-chain/aromatic amino acids among new-onset type-2 diabetes mellitus patients are potentially skewed diabetes predictors

BACKGROUND The lack of specific predictors for type-2 diabetes mellitus(T2DM)severely impacts early intervention/prevention efforts.Elevated branched-chain amino acids(BCAAs:Isoleucine,leucine,valine)and aromatic amino acids(AAAs:Tyrosine,tryptophan,phenylalanine)show high sensitivity and specificity in predicting diabetes in animals and predict T2DM 10-19 years before T2DM onset in clinical studies.However,improvement is needed to support its clinical utility.AIM To evaluate the effects of body mass index(BMI)and sex on BCAAs/AAAs in new-onset T2DM individuals with varying body weight.METHODS Ninety-seven new-onset T2DM patients(<12 mo)differing in BMI[normal weight(NW),n=33,BMI=22.23±1.60;overweight,n=42,BMI=25.9±1.07;obesity(OB),n=22,BMI=31.23±2.31]from the First People’s Hospital of Yunnan Province,Kunming,China,were studied.One-way and 2-way ANOVAs were conducted to determine the effects of BMI and sex on BCAAs/AAAs.RESULTS Fasting serum AAAs,BCAAs,glutamate,and alanine were greater and high-density lipoprotein(HDL)was lower(P<0.05,each)in OB-T2DM patients than in NW-T2DM patients,especially in male OB-T2DM patients.Arginine,histidine,leucine,methionine,and lysine were greater in male patients than in female patients.Moreover,histidine,alanine,glutamate,lysine,valine,methionine,leucine,isoleucine,tyrosine,phenylalanine,and tryptophan were significantly correlated with abdominal adiposity,body weight and BMI,whereas isoleucine,leucine and phenylalanine were negatively correlated with HDL.CONCLUSION Heterogeneously elevated amino acids,especially BCAAs/AAAs,across new-onset T2DM patients in differing BMI categories revealed a potentially skewed prediction of T2DM development.The higher BCAA/AAA levels in obese T2DM patients would support T2DM prediction in obese individuals,whereas the lower levels of BCAAs/AAAs in NW-T2DM individuals may underestimate T2DM risk in NW individuals.This potentially skewed T2DM prediction should be considered when BCAAs/AAAs are to be used as the T2DM predictor.

Effects of vitamin family members on insulin resistance and diabetes complications

The following letter to the editor highlights the article“Effects of vitamin D supplementation on glucose and lipid metabolism in patients with type 2 diabetes mellitus and risk factors for insulin resistance”in World J Diabetes 2023 Oct 15;14(10):1514-1523.It is necessary to explore the role of vitamin family members in insulin resistance and diabetes complications.

Fingerprint Study of Polygonati Rhizoma with Steaming and Exposing to the Sun Alternatively for Different Times

[Objectives]To explore the influence of different times of steaming and exposing to the sun on the fingerprint of Polygonati Rhizoma by studying the HPLC fingerprint of Polygonati Rhizoma processed products with different times of steaming and exposing to the sun,and to provide a basis for the determination of the best processing technology of Polygonati Rhizoma.[Methods]SETSAIL II AQ-C 18(5μm×250 mm×4.6 mm)was used as the column,the column temperature was 30℃,pure water(A)and acetonitrile(B)were eluted gradually,0-10 min,B(5%-10%),10-30 min,B(10%-35%),30-40 min,B(35%-60%),40-45 min,B(60%-100%),flow rate 1 mL/min,absorption wavelength 200 nm.[Results]The relative retained peak area RSDs of the common peaks in the precision,reproducibility and stability tests were all less than 5%.There were 17 common peaks in the fingerprint of nine batches of samples,and the retention time of Peak 2 was basically the same as that of the reference peak of 5-HMF.Peak 4 mainly existed in the chromatogram of Sample 3 to Sample 5,peaks 5 and 11 mainly existed after Sample 3,peaks 9,14 and 16 mainly existed after Sample 6,and peaks 12 and 17 mainly existed after Sample 4.[Conclusions]A total of 17 common peaks were obtained,and the Peak 2 was the designated peak,and the chemical components of each processed product were different.

Time-Frequency System Builds and Timing Strategy Research of VHF Band Antenna Array

VHF (Very High Frequency) band antenna array will receive analog signal from universe for storage after digital sampling and adding time scale, and then do the interference analysis of different sub-station digital signal. It requires the time-frequency system with high precision and low drifting. This paper explains a time-frequency system of VHF band antenna, which can produce standard 10 MHz signal and clock signal needed by sampler, to ensure that two computers which sampling data has the same system time and the storage data has the accurate time scale, the system includes time comparison programme based on the GPS network timing two different sampling control computers. Timing strategy uses a time comparison software which based on the Labview graphical programming platform. This software captures the system time of two computers to analyze and determine the time deviation when the two computers occurs time offset, and then grant the GPS time of NTP server to the two computers through local area network in this time deviation. Final results show that this method can automatically calibrate the system time of the computers in the LAN, Precision Can Reach 0.1 s Orless.

Searching for the Nano-Hertz Stochastic Gravitational Wave Background with the Chinese Pulsar Timing Array Data ReleaseⅠ

Observing and timing a group of millisecond pulsars with high rotational stability enables the direct detection of gravitational waves(GWs).The GW signals can be identified from the spatial correlations encoded in the times-of-arrival of widely spaced pulsar-pairs.The Chinese Pulsar Timing Array(CPTA)is a collaboration aiming at the direct GW detection with observations carried out using Chinese radio telescopes.This short article serves as a“table of contents”for a forthcoming series of papers related to the CPTA Data Release 1(CPTA DR1)which uses observations from the Five-hundred-meter Aperture Spherical radio Telescope.Here,after summarizing the time span and accuracy of CPTA DR1,we report the key results of our statistical inference finding a correlated signal with amplitude logA_(c)=-14.4_(-2.8)^(+1.0)for spectral index in the range ofα∈[-1.8,1.5]assuming a GW background(GWB)induced quadrupolar correlation.The search for the Hellings–Downs(HD)correlation curve is also presented,where some evidence for the HD correlation has been found that a 4.6σstatistical significance is achieved using the discrete frequency method around the frequency of 14 n Hz.We expect that the future International Pulsar Timing Array data analysis and the next CPTA data release will be more sensitive to the n Hz GWB,which could verify the current results.

生物胶改良黏土抗崩解特性试验研究

为研究生物胶对黏土抗崩解性的影响,采用黄原胶、瓜尔胶及黄原胶与瓜尔胶质量比为1∶1混合的复合胶对黏土进行改良,通过抗崩解试验,探究生物胶的含量和种类、压实度对改良黏土抗崩解特性的影响规律,并结合扫描电镜试验进一步分析了生物胶的改良机理。结果表明:生物胶通过增强土体颗粒之间黏聚力增强改良黏土的抗崩解特性,其崩解破坏过程可分为稳定前崩解阶段、稳定崩解阶段及稳定后崩解阶段;随着生物胶含量的增大,改良黏土稳定前崩解阶段延长,崩解速率减缓,崩解率降低;复合胶改良黏土抗崩解特性在质量分数大于1.0%后强于单一生物胶;随着压实度的提升,试样抗崩解特性明显改善。

Austenite grain growth of medium-carbon alloy steel with aluminum additions during heating process

In this study, the effects of heating temperature(850–1100°C) and holding time(30–150 min) on the grain growth behavior of austenite in medium-carbon alloy steel were investigated by conducting experiments. The abnormal grain growth and mixed grain structure phenomenon are explained using an equilibrium precipitation phase diagram calculated by Thermo-Calc software package. The Al N particles were observed by field-emission scanning electron microscopy(FESEM), and the amount of AlN precipitations was detected by electron probe microanalysis(EPMA). Based on the research results, it was found that the average grain size of austenite in the test steel increased continuously with the increase of temperature and holding time. Furthermore, the abnormal growth of austenite occurred in the test steel at 950°C, and the heating temperature affected the austenite grain size more significantly. In addition, the decline in the amount of AlN second-phase particle in the test steel, which weakened the "pinning" effect on austenite grain boundaries, resulted in abnormal growth and the development of mixed austenite grain structures. The prediction model for describing the austenite grain growth of medium-carbon alloy steel during heating was established by regression analysis of the experimental data, and the model was verified to be highly accurate.

Apoptosis induced by preoperative oral 5′-DFUR administration in gastric adenocarcinoma and its mechanism of action

AIM： To study the apoptosis induced by preoperative oral 5＇-DFUR administration in gastric adenocarcinorna and its mechanism of action. METHODS： Sixty gastric cancer patients were divided randomly into three groups （20 each group） before operation： group one： 5＇-DFUR oral administration at the dose of 800-1200mg/d for 3 - 5 d, group two： 500mg 5-FU ± 200 mg/d CF by venous drip for 3 - 5 d, group three （control group）. One or two days after chemotherapy, the patients were operated. Fas/FasL, PD-ECGF and PCNA were examined by immunohistochemistry and apoptotic tumor cells were detected by in situ TUNEL method. Fifty-four patients received gastrectomy, including 12 palliative resections and 42 radical resections. Six patients were excluded. Finally 18 cases in 5＇-DFUR group, 16 cases in CF ± 5-FU group, and 20 cases in control group were analyzed. RESULTS： There was no significant difference in patient mean age, gender, white blood cell count, haernatoglobin （HB）, thromboplastin, perioperative complication incidence, radical or palliation resection, invasion depth （T）, Iymphonode involvement （N）, metastasis （M） and TNM staging among the three groups. However, the PCNA index （PI） in 5＇-DFUR group （40.51 ± 12.62） and 5-FU ±CF group （41.12± 15.26） was significantly lower than that in control group （58.33± 15.69） （F=9.083, P= 0.000）. The apoptotic index （AI） in 5＇-DFUR group （14.39 ±9.49） and 5-FU±CF group （14.11±9.68） was significantly higher than that in control group （6.88 ± 7.37） （F= 4.409, P= 0.017）. The expression rates of Fas and FasL in group one and group three were 66.7% （12/18） and 50% （9/18）, 43.8% （7/16） and 81.3% （13/16）, 45.0% （9/20） and 85% （17/20）, respectively. The expression rate of FasL in 5＇-DFUR group was significantly lower than that in the other two groups （X^2=6.708, P= 0.035）. Meanwhile, the expression rate of PD-ECGF was significantly lower in 5＇-DFUR group （4/18, 28.6%） than in CF±5-FU group （9/16, 56.3%） and control group （13/20, 65.0%） （7,2= 7.542, P= 0.023）. The frequency of Fas expression was significantly correlated with palliative or radical resection （X^2= 7.651, P= 0.006）, invasion depth （X^2= 8.927, P= 0.003）, lymphatic spread （7,2= 4.488, P=0.034） and UICC stages （X^2=8.063, P=0.045） respectively. By the end of March 2005, 45 patients were followed up. The 0.5-, 1-, 2-, 3-year survival rates were 96%, 73%, 60%, 48%, respectively, which were related with T, N, M and Fas expression, but not with PDECGF and FasL expression. CONCLUSION： Preoperative oral 5＇-DFUR administration may induce apoptosis of gastric carcinoma cells and decrease tumor cell proliferation index, but cannot improve the prognosis of patients with gastric cancer. Downregulation of FasL and PD-ECGF expression mediated by 5＇-DFUR may be one of its anti-cancer mechanisms. Fas expression correlates with the progression of gastric carcinoma and may be an effective prognostic factor.

Application of potassium ferricyanide in the spectrophotometric determination of captopril

A novel method for the determination of captopril by spectrophotometer is described in this paper. The experiment is based on the fact that Fe（Ⅲ） is reduced to Fe（Ⅱ） by captopril, then the in sire formed Fe（Ⅱ） reacts with potassium ferricyanide to give the soluble prussian blue at pH 4.00, and its maximal adsorption wavelength （λmax） is 735 nm. Good linear relationship is obtained between the absorbance and the concentration of captopril in the wide range of 0.05-20 μg/mL. The linear regression equation is A = -0.04314 ＋ 0.11423C （μg/mL） with a correlation coefficient R = 0.9998. The detection limit （3σ/k） is 0.04 μg/mL, the molar absorption coefficient is 2.5×10^4 L/mol cm. By mensurating the absorbance of soluble prussian blue, the indirect determination of captopril can be obtained. This method has been successfully applied to determination of captopril in pharmaceutical samples. Analytical results obtained are satisfactory.

Teeming stream protection using an argon shroud during casting of steel ingots

Two kinds of argon shroud protection devices with two different basic structures were designed and investigated. Industrial experiments and numerical simulations were used to examine the protection effect, and the mechanism of air entrapment during the casting of steel ingots was analyzed. The influence of the structure of the argon shroud protection device on the protection effect was investigated. An argon shroud protection device mounted to the nozzle holder on the bottom of the ladle does not provide a good protection effect because air can easily flow into the teeming system and cause reoxidation of molten steel during teeming. By contrast, an argon shroud protection device seated on the top of the central trumpet provides an excellent protection effect, where air has little chance of flowing into the teeming system during casting. The feasibilities of the argon shroud protection devices are discussed.

Blockage of the habenular nucleus can eliminate dyspnea induced by electrostimulation of the insular cortex

BACKGROUND： The insular cortex and habenular nucleus may be a regulatory center for obstructive sleep apnea syndrome, and dyspnea may be caused by insular cortex activity. The insular cortex is a cortical representation of obstructive sleep apnea syndrome. The habenular nucleus is a station for descending insular cortex activity. OBJECTIVE： Through actively stimulating the rat insular cortex, to observe rat respiratory movement, myoelectric activities of genioglossus, arterial partial pressure of oxygen, partial pressure of carbon dioxide and acidity-alkalinity, and to verify a hypothesis that the insular cortex is a superior-position regulation center, and the habenular nucleus is an inferior-position nervous nuclei of the insular cortex in patients with obstructive sleep apnea syndrome. DESIGN, TIME AND SETTING： The randomized, controlled animal study was performed at the Laboratory of Electrophysiology, Department of Physiology, Norman Bathune College of Medicine, Jilin University, China from September 2004 to June 2008. MATERIALS： We used L-glutamic acid （Dingguo Biological Product Research Center, Beijing, China）, lidocaine hydrochloride （Seventh Pharmacy Co., Ltd., Wuxi, China）, electric stimulator （Nihon Kohden, Japan）, and an AVL-OPTI blood gas analyzer （AVL Scientific Co., Roswell, GA, USA）. METHODS： The insular cortex of healthy adult Wistar rats underwent electrostimulation and L-glutamic acid stimulation to record changes in the myoelectric activity of genioglossus and respiratory movement. Some rats were injected with lidocaine to block the habenular nucleus before electrostimulation or L-glutamic acid stimulation. L-glutamic acid and lidocaine were injected by microelectrodes embedded in nuclear groups. MAIN OUTCOME MEASURES： Myoelectric activities of genioglossus, arterial partial pressure of oxygen, partial pressure of carbon dioxide and acidity-alkalinity were measured following apnea in rats undergoing electrostimulation in the insular cortex and following blockade of the habenular nucleus. RESULTS： Following electrostimulation and L-glutamic acid stimulation, rats developed apnea or respiratory rhythm disorders. Simultaneously, the amplitude of myoelectric activity of the genioglossus was reduced （P 〈 0.01 ）, and the electromyogram integral was decreased （P 〈 0.01）. Arterial blood gas analysis showed arterial blood acidosis, a decrease in pH （P 〈 0.05）, and an increase in the negative value of alkaline reserve （P 〈 0.01）. Lidocaine in the habenular nuclear blocked respiratory and other index changes after insular cortex stimulation. CONCLUSION： Dyspnea induced by stimulating the insular cortex may require the habenular nucleus. Paralysis of the habenular nucleus can completely eliminate insular cortex stimulation-induced dyspnea.

VLBI observations with the Kunming 40-meter radio telescope

The Kunming 40-meter radio telescope is situated in the yard of the Yunnan Astronomical Observatory （Longitude： 102.8° East, Latitude： 25.0° North） and saw its first light in 2006 May. The Kunming station successfully joined the VLBI tracking of China＇s first lunar probe ＂Chang＇E-1＂ together with the other Chinese telescopes： the Beijing Miyun 50-meter radio telescope, Urumqi Nanshan 25-meter radio telescope, and Shanghai Sheshan 25-meter radio telescope, and received the downlinked scientific data together with the Miyun station from October of 2007 to March of 2009. We give an introduction to the new Chinese VLBI facility and investigate its potential applications. Due to its location, the Kunming station can significantly improve the u - v coverage of the European VLBI Network （EVN）, in particular, in long baseline observations. We also report the results of the first EVN fringe-test experiment of N09SX1 with the Kunming station. The first fringes in the European telescopes were successfully detected at 2.3 GHz with the ftp-transferred data on 2009 June 17. From scheduling the observations to performing the post correlations, the Kunming station shows its good compatibility to work with the EVN. The imaging result of the extended source 1156＋295 further demonstrates that the Kunming station greatly enhances the EVN performance.

Competitiveness of alga Microcystis aeruginosa co-cultivated with cyanobacterium Raphidiopsis raciborskii confirms its dominating position

Microcystis aeruginosa has always been regarded as the main culprit of cyanobacterial blooms in freshwater.However,in recent years,Raphidiopsis raciborskii has gradually replaced M.aeruginosa as the culprit of cyanobacterial blooms in some tropical and subtropical shallow lakes.To reveal which one plays a more dominant role,interactions between cylindrospermospin(CYN)-producing R.raciborskii and microcystins(MCs)-producing or non-MCs-producing M.aeruginosa strains were studied using bialgal cultures at different initial ratios of biomasses of the two species at 25℃.During the co-cultivation,the M.aeruginosa strains inhibited the growth and heterocyst formation of R.raciborskii filaments,and thus occupied a dominant position during the co-cultivation regardless of the initial biomass ratios in the cultures.In addition,the MCs-producing M aeruginosa strain contributed to a higher portion of the total biomass and exerted a stronger inhibitory effect on R.raciborskii compared with the non-MCs-producing strain.However,the growth of both MCs-producing and non-MCs-producing M.aeruginosa strains was stimulated by R.raciborskii in the co-cultures compared with M.aeruginosa monoculture,indicating that M.aeruginosa could outcompete R.raciborskii if given enough time,enabling it to develop into the dominant species even in very low initial concentration.To our best knowledge,this is the first report on the loss of heterocyst formation by a species of cyanobacteria that resulted from interactions between two different species of cyanobacteria.These findings indicate that it is difficult for R.raciborskii to replace the dominant position of M.aeruginosa under the experimental environmental condition,and the allelopathic effects of M.aeruginosa on R.raciborskii could significantly contribute to the success of M.aeruginosa.

Role of 5-hydroxytryptamine expression in cerebellar Purkinje cells in obstructive sleep apnea syndrome

In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, arterial blood gas analysis showed arterial blood acidosis, reduced pH values, increased alkali reserve negative values, decreased peripheral blood 5-hydroxytryptamine content, and increased 5-hydroxytryptamine expression in cerebellar Purkinje cells. Following lidocaine injection to block the habenular nucleus, abnormalities in breath, genioglossal electromyogram, and blood gas values disappeared, and peripheral blood 5-hydroxytryptamine content returned to levels prior to electric stimulation. However, 5-hydroxytryptamine expression in cerebellar Purkinje cells remained high. The results suggested that 5-hydroxytryptamine expression in Purkinje cells did not correlate with ventilation function involving insular cortex and habenular nucleus.

5-hydroxytryptamine-mediated apnea caused by the habenular nucleus

5-hydroxytryptamine contributes to the control of activities of the dilator muscle in the upper respiratory tract, and is derived from the raphe nuclei, in which the habenular nucleus exerts a sustaJned inhibitory effect. In the present study, respiratory motion curve of the genJoglossus muscle and peripheral 5-hydroxytryptamine changes were observed following L-glutamate stimulation of the habenular nucleus of adult Wistar rats. Results showed that the rats had apnea and decreased plasma 5-hydroxytryptamine content after the neurons in habenular nucleus were excited. Genioglossus muscle electromyogram amplitude and integral were significantly reduced. The genioglossus myoelectric activity and respiratory motion curve were similar to obstructive sleep apnea syndrome, thus confirming that the habenular nucleus is the key nucleus involved in the pathogenesis of obstructive sleep apnea syndrome, and is the primary regulated center in the raphe nuclei. Stimulation of the habenular nucleus may suppress 5-hydroxytryptamine release and result in apnea, which is similar to obstructive sleep apnea syndrome.

Harmful algal bloom-forming dinofl agellate Prorocentrum donghaiense inhibits the growth and photosynthesis of seaweed Sargassum fusiformis embryos

Harmful algal bloom(HAB)is an ecological disaster to local mariculture.At present,its impact on macrophytes has not been well studied.In this study,we cultivated sexually propagated embryos of S argassum fusiformis-an edible seaweed-in Prorocentrum donghaiense suspensions at different cell densities(0,0.50×10^(5),0.75×10^(5),1.00×10^(5),and 1.50×10^(5) cells/mL)for 10 days,during which growth and photosynthetic activities of the embryos were determined,and a monocultivation was set up for comparison.Results show that the relative growth rate and photosynthetic activities of the embryos co-cultivated with P.donghaiense were inhibited mostly and significantly in the cell densities of 0.75×10^(5),1.00×10^(5),and 1.50×10^(5) cells/mL,and the inhibitory effects increased in overall with increased cell densities.The maximum relative electron transport rates(rETR max)and apparent photosynthetic efficiency(a)of co-cultivated embryos were all significantly lower than monocultivation ones on the 10 th day.Furthermore,the photosynthetic activity detected by chlorophyll-a fluorescence transient(i.e.,OJIP),the electron transport among electron transfer accepters of PSII(photosystem II)and that from PSII to PSI(photosystem I)was restricted,which is probably responsible for the decreases of rETR max andain the co-cultivated embryos.In addition,parts of the photosynthetic reaction centers of PSII in the co-cultivated embryos were inactivated.Therefore,P.donghaiense bloom could restrain the development and photosynthetic activities of S.fusiformis embryos,reduce the seedlings stock,and eventually hinder the development of S.fusiformis production industry.

Study of the genus Oxaenanus Swinhoe(Lepidoptera:Erebidae:Herminiiae)from China,with description of a new species

Oxaenanus scopigeralis(Moore,1867),sp.rev.&comb.nov.,the type locality:India,is revalidated from a junior synonym of O.brontesalis(Walker,1858),the type locality:Borneo.The holotype,and male and female genitalia of O.scopigeralis are illustrated,and O.yunnana Zhang&Han,2016 from Yunnan,China is synonymized with O.scopigeralis.This species is newly recorded from Nepal.A new species,Oxaenanus hainana sp.nov.,closely related to O.scopigeralis,is described from Hainan,China by the weaker and fuzzier subterminal lines of both wings,shorter and slenderer cucullus,and more complicated vesica.

苏州—无锡—常州地区地面沉降及地裂缝防控研究

苏州—无锡—常州(苏锡常)地区曾是中国地面沉降灾害最严重的地区之一,从20世纪70年代开始发生地面沉降,随之因差异沉降诱发地裂缝灾害,21世纪以来沉降速率逐年趋缓,部分地区出现区域性的地面回弹,独特的地面沉降发展历程为全面解读地面沉降提供了理想的窗口。为揭示苏锡常地区地面沉降生命周期过程及其驱动机制,利用长时间序列、大区域尺度的三维渗流、应力、应变多场监测数据以及物理试验模型、数值模拟等技术对区域地面沉降与地裂缝宏观演变规律、成因机理进行综合分析。研究结果显示:苏锡常地区地面沉降经历了发生、快速发展、趋缓、滞后和反弹5个阶段;地面沉降与地下水开采密切相关,其地层变形主要来自于地下水开采导致的含水层和弱透水层的压密释水,主采含水砂层及相邻隔水层为沉降主要贡献层,并识别了地层压缩、回弹的时空演变特征及其对地面沉降的贡献;地裂缝是地面沉降发展到一定阶段后所产生的次生地质灾害,其空间展布及成灾时间与地下水水位、地面沉降、基岩起伏变化以及土层结构差异等因素密切相关,提出了驱动地裂缝演化的压—拉—剪—弹物理过程,识别出了地裂缝发生的触发机制和临界条件。同时建立了以地质钻孔全断面光纤监测为特色、多种技术方法融合的“天-空-地”立体化、地下水-地面沉降-地裂缝协同的监测体系,为地面沉降防控提供科学、详细的数据支撑;并创新区域-场地双尺度有限元耦合界面元法,成功实现了三维复杂地质环境条件下地层形变特征及地裂缝生成和扩展的力学机制模拟,为地面沉降、地裂缝易发区精准圈定与防控提供了解决路径;通过总结基于技术创新支撑政府实施的地下水限采、禁采等地面沉降防控实践及其成效,为中国其他省/市地面沉降防控与地下水资源管理起到示范作用。

Variation and optimization of acid-dissolved aluminum content in stainless steel

As a key step in secondary refining, the deoxidation process in clean stainless steel production is widely researched by many scholars. In this study, vacuum oxygen decarburization（VOD） deoxidation refining in a 40-t electric arc furnace ＋ VOD ＋ ingot casting process was analyzed and optimized on the basis of Al deoxidation of stainless steel and thermodynamic equilibrium reactions between the slag and steel. Under good stirring conditions in VOD, the deoxidation reaction reaches equilibrium rapidly, and the oxygen activity in the bulk steel is controlled by the slag composition and Al content. A basicity of 3–5 and an Al content greater than 0.015wt% in the melt resulted in an oxygen content less than 0.0006wt%. In addition, the dissolved oxygen content decreased slightly when the Al content in the steel was greater than 0.02wt%. Because of the equilibrium of the Si–O reaction between the slag and steel, the activity of SiO2 will increase while the Si content increases; thus, the Si content should be lowered to enable the formation of a high-basicity slag. A high-basicity, low-Al2O3 slag and an increased Si content will reduce the Al consumption caused by SiO2 reduction.