Experimental Research of Tankyrase1 Antisense Oligodeoxynucleotides on the Proliferation of Lung Cancer Cell Nodules

OBJECTIVE To observe the effects of sense and antisense oligodeoxynucleotides of tankyrase 1 （TANK1-SODN and TANK1-ASODN） on murine tumor growth following intratumoral injection, investigate the actual suppressing result and mechanism of TANK1-ASODN on cancer cell proliferation, and discuss the possibility of using it in gene therapy on human lung cancer cells. METHODS After BALB/c nude mice had been subcutaneously inoculated with human lung cancer cell line CALU and it had grown into tumor nodules, we distributed these mice randomly into 3 groups： 4 in saline treatment group, and 5 each in TANK1-SODN group, and TANK1-ASODN groups. Then multiple direct intratumoral injections of synthesized TANK1-ASODN given continuously into tumor nodules for 16 days, this was compared with TANK1-SODN and saline control groups. During the experiment we measured the tumor volume every 5 days with vernier calipers; observed the histopathological characteristics of tumor tissues under microscope; went further to detect the minute changing of ultrastructure of cancer cells by electron microscope; tested the expression levels of ki67 and hTERT protein by means of SABC immunohistochemical method; and detected the lung cancer cells＇ hTERT mRNA expression level by hybridization in situ （ISH） in each group. RESULTS After 16 days of continuous injection, the tumor volume in TANK1-ASODN group was significantly smaller than the other 2 groups （both P 〈 0.01）; quite a lot of tumor cell degeneration and necrosis were observed in mice given TANK1-ASODN. The results of electron microscope also showed that TANK1-ASODN has the power to kill cancer cells in various ways. Moreover, statistically signi.cant decreases in the positive expression ratio of Ki67 Labeling Index （P 〈 0.01）, hTERT protein （P 〈 0.01）, and hTERT mRNA （P 〈 0.01） were consistently observed in the TANK1-ASODN group. CONCLUSION Human lung cancer cell line CALU expressed high telomerase activity. TANK1-ASODN had the ability to decline the high expression level of hTERT; inhibit the activity of telomerase, accelerate tumor cell degeneration and necrosis; and then suppress the proliferation of cancer cells.

Application of Landscape Plants in the Huaxi National Urban Wetland Park

The current situation of plants in Shilihe Beach of the Huaxi National Urban Wetland Park was investigated and analyzed. The results show that there are about 181 species of landscape plants in this park, of which arbors and shrubs are dominant. Various species of plants grow differently because of different types of artificial maintenance. The overall partition of the wetland park dose not follow the features of a river-type wetland and is not clear. In this park, the planting area of hygrophyte is far less than that of terrestrial plants. As a result, the wetland park is evolving into a public garden. It is suggested that the overall design of the park should be conducted in accordance with site characteristics and resources, and the park should be developed and built reasonably based on the principle that the ecology is a priority.

Realization of -bit semiclassical quantum Fourier transform on IBM's quantum cloud computer

To overcome the difficulty of realizing large-scale quantum Fourier transform(QFT) within existing technology, this paper implements a resource-saving method(named t-bit semiclassical QFT over Z_(2~n)), which could realize large-scale QFT using an arbitrary-scale quantum register. By developing a feasible method to realize the control quantum gate Rk, we experimentally realize the 2-bit semiclassical QFT over Z_(2~3) on IBM's quantum cloud computer, which shows the feasibility of the method. Then, we compare the actual performance of 2-bit semiclassical QFT with standard QFT in the experiments.The squared statistical overlap experimental data shows that the fidelity of 2-bit semiclassical QFT is higher than that of standard QFT, which is mainly due to fewer two-qubit gates in the semiclassical QFT. Furthermore, based on the proposed method, N = 15 is successfully factorized by implementing Shor's algorithm.

温经汤对大鼠子宫内膜异位症SPARC介导的上皮-间质转化的影响

目的探讨温经汤对子宫内膜异位症(EMT)大鼠模型富含半胱氨酸的酸性分泌蛋白(SPARC)介导的上皮-间质转化的影响。方法采用手术自体移植子宫内膜块法建立EMT大鼠模型,将建模成功的40只大鼠随机分为模型组(给予质量分数为0.9%的氯化钠溶液10 mL/kg)、孕三烯酮组(给予孕三烯酮溶液0.5 mg/kg)以及温经汤低、中、高剂量组(分别给予温经汤免煎颗粒剂8.19 g/kg、16.38 g/kg、32.76 g/kg),每组各8只,另取7只未手术大鼠作为正常对照组(给予质量分数为0.9%的氯化钠溶液10 mL/kg)。所有大鼠均连续灌胃给药3周后进行取材。腹主动脉取血,采用酶联免疫吸附试验(ELISA)法检测血清中SPARC水平。电子游标卡尺测量异位病灶体积,计算生长抑制率。取下异位病灶组织(正常对照组取下双侧子宫组织)采用免疫组织化学法检测内膜组织中SPARC、锌指转录因子(Snail)、E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)的表达,采用Western blot法和RT-qPCR法测定内膜组织中上述因子的蛋白和mRNA相对表达量。结果各治疗组异位病灶的生长抑制率与模型组比较差异均有统计学意义(P<0.05)。与正常对照组比较,模型组血清中SPARC水平显著升高(P<0.05),各治疗组血清中SPARC水平均明显低于模型组(P<0.05)。模型组异位内膜中SPARC、Snail、Vimentin呈高表达,E-cadherin呈低表达,经温经汤治疗后,异位内膜中SPARC、Snail、Vimentin的阳性表达减少,E-cadherin的阳性表达增加。温经汤中、高剂量组和孕三烯酮组异位内膜中SPARC、Snail、Vimentin的蛋白相对表达量均明显低于模型组(P<0.05),E-cadherin的蛋白相对表达量显著高于模型组(P<0.05)。温经汤高剂量组和孕三烯酮组异位内膜中Snail、Vimentin mRNA相对表达量均较模型组显著降低(P<0.05),E-cadherin mRNA相对表达量明显高于模型组(P<0.05)。结论温经汤可以降低EMT大鼠模型血清中SPARC水平,下调异位内膜中SPARC、Snail、Vimentin蛋白和mRNA表达,升高异位内膜中E-cadherin蛋白和mRNA表达,通过调控SPARC介导的上皮-间质转化,从而抑制异位内膜病灶的生长。

UPLC测定食用油中的3种香兰素类物质含量

为解决现有检测方法存在前处理净化不完全、不彻底的情况,建立了超高效液相色谱(UPLC)测定食用油中香兰素、甲基香兰素和乙基香兰素含量的分析方法。用乙腈提取食用油中目标物,以乙腈饱和的正己烷萃取净化,冷冻分层后,再经OasisPRiMEHLB固相萃取柱净化,采用C18色谱柱分离,以0.5%甲酸水溶液-乙腈为流动相进行UPLC测定,外标法定量。结果表明:3种香兰素类物质在0.1~100.0mg/L范围内线性关系良好,相关系数大于0.999,检出限均为0.03 mg/kg,定量限均为0.1mg/kg;在0.1、0.2、1.0mg/kg3个添加水平的平均回收率为86.6%~108.1%,相对标准偏差(RSD)为2.0%~8.8%。同时,采用本方法与BJS201705中方法对食用油中的3种香兰素类物质进行测定,两种方法结果间无显著性差异。综上,建立的方法采用两步净化,减少了杂质对香兰素类物质的干扰,适用于食用油中香兰素类物质含量的测定。

经腹超声引导下经皮穿刺活检对内镜超声引导下穿刺活检诊断胰腺肿瘤的补充价值

目的 观察经腹超声引导下经皮穿刺活检对内镜超声(EUS)引导下穿刺活检诊断胰腺肿瘤的补充价值。方法 回顾性分析30例因EUS引导下穿刺活检诊断结果不满意(未见肿瘤细胞20例、可见异常细胞但无法明确诊断10例)而接受经腹超声引导下经皮穿刺活检的胰腺肿瘤患者,评价后者的补充诊断价值。结果 20例EUS引导下穿刺活检未见肿瘤细胞患者中,经腹超声引导下穿刺活检明确诊断11例肿瘤及其病理类型,7例可见异常细胞但未能明确诊断,2例仍未见肿瘤细胞;10例EUS引导下穿刺结果无法明确诊断患者中,经腹超声引导下穿刺活检明确诊断9例肿瘤及其病理类型,1例为非典型细胞。结论 经腹超声引导下经皮穿刺活检对EUS引导下穿刺活检诊断胰腺肿瘤具有较高补充价值。

地诺孕素片在中国健康成年女性受试者空腹状态下的生物等效性研究

目的评价地诺孕素片在中国健康成年女性受试者空腹状态下的生物等效性和安全性。方法采用单中心、随机、开放、两周期、双交叉给药试验设计,首都医科大学附属北京安贞医院2022年2月21日至3月3日入选24例中国健康成年女性受试者,采用随机数字表法分为2组(参比制剂-受试制剂组和受试制剂-参比制剂组),每组12人。2组服药顺序不同,分别在第1天和第8天,先后空腹状态下服用1次参比制剂和受试制剂,或受试制剂和参比制剂(地诺孕素片2 mg)。通过采集生物样本并进行血药浓度分析,对比两药品药代动力学参数,并进行生物等效性评价,同时观察受试者的临床安全性。结果空腹口服受试制剂和参比制剂的药代动力学参数:血药峰浓度分别为(52±13)、(54±11)μg/L,达到峰浓度的时间分别为1.75(0.50,5.50)、1.00(0.50,5.50)h,从0时至t时的血药浓度-时间曲线下面积分别为(620±87)、(604±99)h·μg/L,从0时至无穷大时的血药浓度-时间曲线下面积分别为(644±97)、(628±111)h·μg/L,血浆消除半衰期分别为(9.7±1.5)、(9.8±1.8)h。主要药代动力学参数的几何均数比值均落在生物等效的80%~125%范围内。所有受试者在研究期间均未发生可能导致退出研究的不良事件和严重不良事件。结论地诺孕素片受试制剂和参比制剂在中国健康成年女性受试者空腹状态下具有生物等效性,并具有较高的安全性。

压电陶瓷作动器的卡尔曼滤波输出反馈预测控制

为了抑制压电陶瓷的迟滞、非线性特性对压电微定位平台精度的影响,建立反映压电陶瓷作动器频率依赖迟滞非线性特性的Hammerstein模型:利用非对称Bouc-Wen模型来表示静态迟滞非线性和利用动态线性模型表征信号频率的影响。针对迟滞补偿器不能完全的进行补偿,并且存在噪音干扰实验设备,采用基于卡尔曼滤波的预测控制,提高压电微定位平台的控制精度。预测控制用来减小逆补偿误差以及建模误差等模型不确定性的影响,卡尔曼滤波用来获得系统的状态估计值。实验结果显示,该控制器在正弦波信号下的相对跟踪误差小于0.68%,三角波信号下相对跟踪误差小于0.70%。在迟滞补偿的基础上,采用卡尔曼滤波的预测控制,该方法可以有效地完成对微定位平台的高精度跟踪。

Adsorption equilibrium, kinetics, and dynamic separation of Ca2+ and Mg2+ ions from phosphoric acid–nitric acid aqueous solution by strong acid cation resin

The separation of Ca2+and Mg2+ions from phosphoric acid-nitric acid aqueous solution is very significant for the neutralization process of nitrophosphate fertilizer.This paper studied the adsorption equilibrium,kinetics,and dynamic separation of Ca2+and Mg2+ions by strong acid cation resin,and the effects of phosphoric acid and nitric acid on the adsorption process were investigated.The results reveal that the adsorption process of Ca2+and Mg2+ions in pure water on resin is in good agreement with the Langmuir isotherm model and their maximal adsorption capacities are 1.86 mmol·g-1 and 1.83 mmol·g-1,respectively.The adsorption kinetics of Ca2+and Mg2+ions on resin fits better with the pseudo-first-order model,and the adsorption equilibrium in pure water is reached within 10 min contact time,while at the present of phosphoric acid,the adsorption rate of Ca2+and Mg2+ions on resin will go down.The dynamic separation experiments demonstrate that the designed column adsorption is able to undertake the separation of metal ions from the mix acids aqueous solution,but the dynamic operation should control the flow rate of mix acid solution.Besides nitric acid solution was proved to be effective to completely regenerate the spent resin and achieve the recyclable operation of separation process.

Inhibition of self-renewal and differentiation of HT-29 cells-derived cancer stem-like cells by scutellarin via Hedgehog signaling pathway

OBJECTIVE To investigate the inhibitory effect of scutellarin on the self-renewal and differentiation of HT-29 cells-derived cancer stem-like cells(HT-29CSC)in vitro and in vivo,and to explore its mechanism.METHODS The effect of scutellarin on the growth of HT-29CSC was determined by 3D Culture assay.The effect of scutellarin on growth and transformation of HT-29CSC was probed by soft agar colony formation assay.The effect of scutellarin on the differentiation of HT-29CSC was determined by serum induction differentiation assay in vitro.The effects of scutellarin on the expressions of marker gene Lgr5,target gene c-Myc,proliferation gene CK20 and Nanog gene were measured by quantitative real-time RT-PCR.Investigate the effect of scutellarin on the expression of c-Myc,Gli1,and Lgr5 protein by Western blotting.A subcutaneous xenograft model of colon cancer in nude mice was established and administered by intraperitoneal injection.The change of body weight and tumor size of nude mice were observed every two days.Investi⁃gate the effects of scutellarin on the growth of xenograft tumors in nude mice.The expression of CD133,Lgr5,Gli1,Ptch1,c-Myc,Ki67,CK20,Nanog gene in tumors were measured by quantitative real-time RT-PCR.The expression of c-Myc,Gli1,Lgr5,CD133,Ki67 protein were measured by Western blotting.RESULTS Scutellarin can inhibit the growth of HT-29CSC in 3D culture.Compared with the solvent control group,scutellarin can significantly inhibit the growth and transformation and differentiation of HT-29CSC in vitro(P<0.01).The expression levels of marker genes Lgr5,target gene c-Myc,proliferation gene CK20 and Nanog in HT-29CSC were down-regulated by scutellarin.Scutellarin can reduce the expression of c-Myc,Gli1,and Lgr5 protein in HT-29CSC.Scutellarin can inhibit the growth of colon cancer xenografts,lower CD133,Lgr5,Gli1,Ptch1,c-Myc,Ki67,CK20,and Nanog mRNA level of xenograft tumors,reduce the expression of c-Myc,Gli1,Lgr5,CD133,and Ki67 protein of xenograft tumors in nude mice.CONCLUSION Scutellarin,which is the main component of scutellaria barbata,can inhibit the differentiation of HT-29CSC and the mechanism is to inhibit the activity of Hedgehog signaling pathway.

Ground state cooling of an optomechanical resonator with double quantum interference processes

We present a cooling scheme with a tripod configuration atomic ensemble trapped in an optomechanical cavity.With the employment of two different quantum interference processes,our scheme illustrates that it is possible to cool a resonator to its ground state in the strong cavity-atom coupling regime.Moreover,with the assistance of one additional energy level,our scheme takes a larger cooling rate to realize the ground state cooling.In addition,this scheme is a feasible candidate for experimental applications.

Pseudolaric acid B extracted from the Chinese medicinal herb Cortex Pseudolaricis ameliorates DNFB-induced atopic dermatitis-like skin lesions in BALB/c mice

Objective:Pseudolaric acid B(PB)is a newly identified diterpenoid isolated from Tujinpi(Cortex Pseudolaricis).In the present study,we aimed to explore the anti-inflammatory effects of PB on atopic dermatitis(AD),as well as the molecular mechanisms underlying its effects.Methods:BALB/c mice treated with 2,4-dinitrofluorobenzene were orally administered with PB(10 mg?kg-1?d-1).After evaluating the AD score,serum levels of IgE and the mRNA expression of NLRP3 inflammasome and IL-1βwere measured by ELISA and qRT-PCR respectively.Results:The results showed that PB treatment significantly ameliorated the development of AD-like clinical symptoms and effectively suppressed the infiltration of inflammatory cells.Furthermore,PB inhibited the expression of NLRP3 inflammasome and IL-1βin skin lesions,and downregulated serum IgE levels.Conclusion:The anti-inflammatory properties of PB were demonstrated using the 2,4-dinitrofluorobenzene-induced mouse model of AD-like skin lesions.Our study highlighted the potential use of PB as a novel therapeutic agent for the treatment of inflammation-associated skin diseases.

苏州艺圃游人观景视点时空分布规律研究

园林文化遗产是凝聚地方建造技艺与文化蕴涵的物质载体。以苏州古典园林艺圃为例,剖析古典园林景观形态特征作用于游人时空分布的内在机理。借助Python爬虫爬取网络用户生成内容(User Generated Content,UGC),根据UGC影像数据所反映的景观类型与观景视点进行信息提取与聚类分析。在此基础上,选取空间类型、景观偏好和季相表征为景观分析指标,将艺圃内各类景观形态特征与游人感知偏好指标间的相关性进行量化分析。初步结果表明,功能属性与空间类型、景观偏好类别、视线连通性、视域范围是影响游人空间分布的影响因子,植物季相特征构成了影响游人时空分布的主要因素。旨在科学量化游人观景视点的时空分布特征,深入解读游人的景观偏好及影响其观景行为时空分布的机制。

Vitamin D Status, Autonomic Nervous System Activity, and Cardiometabolic Risk

Vitamin D and its association with cardiovascular disease (CVD) are currently a topic of investigation. The objective of this study was to explore the association between vitamin D status (serum 25-hydroxyvitamin D (25(OH)D)) and a cardiometabolic risk score (CMRS) derived from markers of cardiac autonomic nervous system activity, vascular dynamics, and body composition, using an innovative non-invasive technology. We found that individuals who were vitamin D sufficient ((25(OH)D) ≥ 30 ng/ml, n = 51), compared to those who were vitamin D insufficient (<30 ng/ml, n = 44), had significantly higher heart rate variability (as measured by time and frequency domain variables) and lower photoplethysmography analysis markers and CMRS. These outcomes show that vitamin D insufficient subjects had reduced cardio protective parasympathetic nervous system activity, increased endothelial dysfunction, and hence were at greater cardiometabolic risk, implying vitamin D may play a meaningful role in CVD.

固相微萃取-气相色谱-质谱法分析油莎豆中挥发性成分

以油莎豆为研究对象,采用固相微萃取-气相色谱-质谱联用法(SPME-GC-MS)测定其挥发物成分。通过优化前处理过程中萃取头类型、萃取温度、萃取时间,确定最佳萃取条件。利用质谱数据库对挥发物成分进行鉴定,并采用归一化法进行含量分析。结果表明:最佳萃取条件为采用DVB/CAR/PDMS萃取头、萃取温度110℃、萃取时间30 min,在此条件下,油莎豆中共检测出27种挥发物,其主要特征挥发物为醇类和醛类物质,其相对含量分别为32.30%和43.56%。

“罐采样-实验室分析”测定环境空气中117种挥发性有机物要点分析

“罐采样-实验室分析”是当前测定环境空气中挥发性有机物的主流方法。结合已有资料,针对方法涉及的采样、样品运输与保存、分析测试等环节,考察了环境空气中挥发性有机物监测的主要影响因素。罐选择、清洁度、气密性、惰性化是样品保存的先决条件;采样流量控制器的气密性、流量校准等是保证监测准确的基本要素;温度变化对样品保存产生影响;标准气体的湿度、配置平衡时间、保存时间、配制准确性和内标气体的保存时间等会导致校准和溯源产生偏差。样品中的水分及二氧化碳是分析过程中影响监测结果准确性的干扰因素,实验室有机溶剂也会干扰分析的准确性。

基于伏邪托透理论探讨再生障碍性贫血病机与治疗

再生障碍性贫血是一种免疫功能紊乱的骨髓造血功能衰竭症,目前诊疗过程中极易出现出血及感染,病情凶险。临床多采用免疫抑制治疗,复发率高,多数患者仍需坚持规律输血;中医药通过益精填髓等治疗对再生障碍性贫血颇有成效,但多着眼于再生障碍性贫血平台期,对其急性发作期的中医药治疗经验不足。通过对“伏邪托透”理论溯源,阐述伏邪托透的内在规律与免疫学逻辑,通过六经与卫气营血辨证,构建“平台期-发作期”的桥梁,针对伏邪托透的不同病位与疾病状态,采取相应的诊治思路,一则通过益精填髓、健运中焦等治法滋养骨髓、扶助正气;一则通过辨证运用具有辛散、托透功效的药物,将有形之邪从骨髓透散至肌表,形成痈肿疮疡,给邪以出路,可逐渐将邪气透尽,降低再生障碍性贫血急性发作频率,减少甚至摆脱输血依赖。其创新之处在于通过观察疾病的动态变化,在药物作用下“主动”将疾病从“平台期”过渡到“发作期”,扶助正气与托透伏邪并施,打破免疫稳态,恢复气机的升降出入,重建机体的阴阳平衡,从根本上解决再生障碍性贫血反复发作这一难题,对完善该病的理论和临证有重要意义。

南海东部底水油藏水平井控水完井技术研究进展

南海东部底水油藏分布广泛,水平井开发方式可大幅度降低底水锥进速度,但由于水平段距离长,储层非均质性和水平井跟趾效应造成的点状见水易导致油井含水率大幅上升,降低生产效益。针对上述问题,南海东部油田采用ICD、AICD、C-AICD等控水工具,结合控水颗粒充填、智能分段分流等控水工艺,开展了底水油藏水平井控水完井技术研究与应用,取得显著效果。围绕南海东部底水油藏水平井控水完井技术研究进展,综合分析了不同控水方式的控水原理、技术特点和不同控水完井工具的特性曲线数学模型,总结了不同生产预测技术的应用规模和不同控水完井技术的应用效果。在此基础上提出了底水油藏水平井控水完井技术挑战和发展趋势,可为南海东部底水油藏水平井控水完井的进一步发展提供参考。

重症肺炎患儿肺部超声检查表现与临床治疗效果的关系

目的 探讨重症肺炎患儿肺部超声检查表现及与患儿临床治疗效果的关系。方法 选取我院收治的重症肺炎患儿80例(重症组),另选取同期治疗的普通肺炎患儿80例(对照组),两组患儿均进行肺部超声检查,对比重症及非重症患儿肺部超声检查表现差异,观察重症组患儿治疗前后肺部超声检查表现变化,并根据疗效分组,对比治疗有效组与治疗无效组肺部超声检查表现差异,完成影响此类患儿临床疗效的Logistic回归分析。结果 重症组B线≥10条、胸腔积液、支气管充气征、胸膜异常征、肺实变征患儿占比显著高于对照组,差异有统计学意义(P<0.05);治疗后重症组患儿B线≥10条、胸腔积液、气管充气征、胸膜异常征、肺实变征占比明显低于治疗前,差异有统计学意义(P<0.05);治疗有效组B线≥10条、胸腔积液、支气管充气征、胸膜异常征、肺实变征患儿占比显著低于治疗无效组,差异有统计学意义(P<0.05);Logistic回归分析证实,B线≥10条、胸腔积液、气管充气征、胸膜异常征、肺实变征均为重症肺炎患儿治疗无效的独立危险因素(均OR>1,P<0.05)。结论 肺部超声检查可用于重症肺炎患儿的诊断鉴别,且通过治疗过程中肺部超声检查表现的变化进行疗效评估。

气道打开体位自动调节器在非紧急气管插管中的临床应用研究

目的:探索气道打开体位自动调节器在非紧急气管插管中的临床应用效果。方法:选取2022年6月至2023年4月在重庆医科大学附属第一医院手术室行择期全麻手术的非紧急气管插管患者312例,并按随机对照原则将患者分为人工对照组和仪器试验组(体位夹角90°组、体位夹角95°组和体位夹角100°组)。比较4组间首次插管成功率、声门视野暴露情况、暴露声门时间、插管时间、尝试插管次数、插管困难量表评分(intubation difficulty scales,IDS评分)以及插管并发症和不良反应发生率的差异。结果:仪器体位夹角100°组的首次插管成功率、声门视野暴露分级(cormack-lehane grade,CL分级)、声门开口百分比评分(the percentage of glottic opening score,POGO评分)、尝试插管次数、暴露声门时间和插管时间均明显优于人工对照组(校正后P<0.05);仪器体位夹角90°组和95°组与人工对照组之间、3组仪器试验组之间的差异无统计学意义(校正后P>0.05);仪器体位夹角95°组和100°组的IDS评分显著低于人工对照组(校正后P<0.05),其他组两两之间的差异无统计学意义(校正后P>0.05);4组在插管并发症的发生率上没有明显差异(P>0.05)。结论:气道打开体位自动调节器在体位夹角90°~100°时均能代替人工方法安全有效地打开非紧急气管插管患者的气道,在体位夹角为100°时能够提高首次插管成功率、改善声门视野暴露、减少尝试插管次数并缩短暴露声门时间和插管时间,在体位夹角95°和100°时能够降低气管插管的困难程度。