Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN...Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN sample by trypsin,the correlative polypeptide fragments GDSVVYGLR and QNLLAPQTLPSK were obtained by FT-ICR-MS.It showed that the method of nano-spray HPLC combined with FT-ICR-MS and Mascot search was very efficient for the analysis of the purified OPN and its polypeptide fragments.Further study provides more academic theories of their different modifications and the relative bioactivities.展开更多
选用肽质量指纹谱(peptide mass fingerprint,PMF)技术鉴定质谱纯海兔肝铁蛋白(liver ferritin ofAplysia,ALF)。来源于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)仪中的激光和基质芥子酸协同解吸海兔肝铁蛋白(ALF)为带双电荷、...选用肽质量指纹谱(peptide mass fingerprint,PMF)技术鉴定质谱纯海兔肝铁蛋白(liver ferritin ofAplysia,ALF)。来源于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)仪中的激光和基质芥子酸协同解吸海兔肝铁蛋白(ALF)为带双电荷、单电荷[M+H]+和二聚体的亚基离子,并可供质谱分析。ALF亚基的质荷比m/z分别为9784.03[M+2H]2+、19678.42[M+H]+和39387.80[2M+H]+,其中亚基分子量[M+H]+略小于鲨鱼肝铁蛋白(liver ferritin of shark,SLF)。在弱碱介质(pH8.0)条件下,电子光谱技术研究指出,抗坏血酸以1/2级反应方式参与ALF释放铁全过程,同时又使ALF以一级反应动力学方式释放铁,呈现两种不同的速率。推测这一异常现象可能与ALF含低铁量、亚基调节能力和海兔的进化地位有关。展开更多
Protein-protein interactions （PPIs） are of vital importance for virtually all processes of a living cell. The study of these associations of protein molecules could improve people＇s understanding of diseases and pr...Protein-protein interactions （PPIs） are of vital importance for virtually all processes of a living cell. The study of these associations of protein molecules could improve people＇s understanding of diseases and provide basis for therapeutic approaches. Although efforts have been devoted to the development of methodology for predicting PPIs and protein interaction networks, the application of most existing methods is limited because they need information about protein homology or the interaction marks of the protein partners.展开更多
The potential of MELC, a brand-new and highly efficient technique for studying the temporal and spatial distribution of proteins in living tissue, has been explored jointly over the last years by Walter Schubert, its ...The potential of MELC, a brand-new and highly efficient technique for studying the temporal and spatial distribution of proteins in living tissue, has been explored jointly over the last years by Walter Schubert, its inventor from the Molecular Pattern-Recognition Research Group at the Institute of Medical Neurobiology, Otto-von-Guericke University （Magdeburg, Germany）, and his collaborators including Andreas Dress from the CASIMPG Partner Institute for Computational Biology （PICB）, the Shanghai Institutes for Biological Sciences（SIBS）.展开更多
文摘Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN sample by trypsin,the correlative polypeptide fragments GDSVVYGLR and QNLLAPQTLPSK were obtained by FT-ICR-MS.It showed that the method of nano-spray HPLC combined with FT-ICR-MS and Mascot search was very efficient for the analysis of the purified OPN and its polypeptide fragments.Further study provides more academic theories of their different modifications and the relative bioactivities.
文摘选用肽质量指纹谱(peptide mass fingerprint,PMF)技术鉴定质谱纯海兔肝铁蛋白(liver ferritin ofAplysia,ALF)。来源于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)仪中的激光和基质芥子酸协同解吸海兔肝铁蛋白(ALF)为带双电荷、单电荷[M+H]+和二聚体的亚基离子,并可供质谱分析。ALF亚基的质荷比m/z分别为9784.03[M+2H]2+、19678.42[M+H]+和39387.80[2M+H]+,其中亚基分子量[M+H]+略小于鲨鱼肝铁蛋白(liver ferritin of shark,SLF)。在弱碱介质(pH8.0)条件下,电子光谱技术研究指出,抗坏血酸以1/2级反应方式参与ALF释放铁全过程,同时又使ALF以一级反应动力学方式释放铁,呈现两种不同的速率。推测这一异常现象可能与ALF含低铁量、亚基调节能力和海兔的进化地位有关。
文摘Protein-protein interactions （PPIs） are of vital importance for virtually all processes of a living cell. The study of these associations of protein molecules could improve people＇s understanding of diseases and provide basis for therapeutic approaches. Although efforts have been devoted to the development of methodology for predicting PPIs and protein interaction networks, the application of most existing methods is limited because they need information about protein homology or the interaction marks of the protein partners.
文摘The potential of MELC, a brand-new and highly efficient technique for studying the temporal and spatial distribution of proteins in living tissue, has been explored jointly over the last years by Walter Schubert, its inventor from the Molecular Pattern-Recognition Research Group at the Institute of Medical Neurobiology, Otto-von-Guericke University （Magdeburg, Germany）, and his collaborators including Andreas Dress from the CASIMPG Partner Institute for Computational Biology （PICB）, the Shanghai Institutes for Biological Sciences（SIBS）.
