1.Introduction The arrangement of sound in different sequences is necessary for the great art of music.It is composed of precisely scheduled quasi-periodic segments of rich harmonic complexity(notes and syllables),int...1.Introduction The arrangement of sound in different sequences is necessary for the great art of music.It is composed of precisely scheduled quasi-periodic segments of rich harmonic complexity(notes and syllables),interrupted by transients,bursts of noise,and/or silence(Varnet et al.,2015).Melody,rhythm,harmony,timbre,shape,and style are some of the different aspects of music(Kemper and Danhauer,2005).The richness of music makes it an ideal subject for studying the workings of the human auditory system.Training effects are also known to improve cortical plasticity,which can strengthen common subcortical networks.A variety of learning methodologies have focused on either a single sensory modality task or more challenging tasks requiring higher-order cognitive abilities and sensorimotor linkages in order to investigate neuroplasticity(Zatorre et al.,2012).(Li et al.,2014;Siuda-Krzywicka et al.,2016).A number of non-musical abilities,such as auditory attention,speech perception in noisy contexts,pitch pattern analysis,temporal resolution,duration discrimination,and auditory working memory,are significantly improved through training(J€ancke,2012;Kraus et al.,2012).展开更多
目的研究腺病毒携带目的基因经完整圆窗膜途径耳蜗转导的可行性及安全性,为内耳基因治疗提供实验基础和理论依据。方法20只白色红目豚鼠,术前及术后分别行听性脑干反应(ABR)检查。实验组(12只)以重组腺病毒携带的增强型绿色荧光蛋白基因...目的研究腺病毒携带目的基因经完整圆窗膜途径耳蜗转导的可行性及安全性,为内耳基因治疗提供实验基础和理论依据。方法20只白色红目豚鼠,术前及术后分别行听性脑干反应(ABR)检查。实验组(12只)以重组腺病毒携带的增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(8只)以人工外淋巴液注入豚鼠圆窗龛内。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片,耳蜗冰冻切片观察。结果于圆窗龛内注入腺病毒携带目的基因的转导方法对听力无明显影响。转染耳蜗及对侧耳蜗内目的基因呈广泛表达。5天组表达产物最高,14天组逐渐降低。对照组耳蜗未见EPFP表达。结论于圆窗龛内注入腺病毒携带目的基因转导的方法对耳蜗无明显毒害作用,且能够将目的基因成功转导至双侧耳蜗组织并广泛表达。展开更多
文摘1.Introduction The arrangement of sound in different sequences is necessary for the great art of music.It is composed of precisely scheduled quasi-periodic segments of rich harmonic complexity(notes and syllables),interrupted by transients,bursts of noise,and/or silence(Varnet et al.,2015).Melody,rhythm,harmony,timbre,shape,and style are some of the different aspects of music(Kemper and Danhauer,2005).The richness of music makes it an ideal subject for studying the workings of the human auditory system.Training effects are also known to improve cortical plasticity,which can strengthen common subcortical networks.A variety of learning methodologies have focused on either a single sensory modality task or more challenging tasks requiring higher-order cognitive abilities and sensorimotor linkages in order to investigate neuroplasticity(Zatorre et al.,2012).(Li et al.,2014;Siuda-Krzywicka et al.,2016).A number of non-musical abilities,such as auditory attention,speech perception in noisy contexts,pitch pattern analysis,temporal resolution,duration discrimination,and auditory working memory,are significantly improved through training(J€ancke,2012;Kraus et al.,2012).
文摘目的研究腺病毒携带目的基因经完整圆窗膜途径耳蜗转导的可行性及安全性,为内耳基因治疗提供实验基础和理论依据。方法20只白色红目豚鼠,术前及术后分别行听性脑干反应(ABR)检查。实验组(12只)以重组腺病毒携带的增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(8只)以人工外淋巴液注入豚鼠圆窗龛内。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片,耳蜗冰冻切片观察。结果于圆窗龛内注入腺病毒携带目的基因的转导方法对听力无明显影响。转染耳蜗及对侧耳蜗内目的基因呈广泛表达。5天组表达产物最高,14天组逐渐降低。对照组耳蜗未见EPFP表达。结论于圆窗龛内注入腺病毒携带目的基因转导的方法对耳蜗无明显毒害作用,且能够将目的基因成功转导至双侧耳蜗组织并广泛表达。
