Alginate oligosaccharides(AOS)and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds.We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction ...Alginate oligosaccharides(AOS)and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds.We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from Lessonia trabeculata,and found that the optimum extraction procedure was:first,using 10-g/L Na_(2)CO_(3) solution to digest the seaweeds for 2 h at 80℃ with the material-liquid ratio 1:20(g/mL),and then adding 4.0%fucoidan cleavage enzyme for reaction for 12 h at 45℃.The yield of AOS and fucoidan was 21.36%and 14.40%,respectively,which was higher than those using traditional methods.The degree of polymerization of AOS was determined to be 3-5,confirming the results through thin layer chromatography(TLC)and electrospray ionization mass spectrometry(ESI-MS).The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97.Through anion-exchange column chromatography,four sulfated polysaccharide fractions(F0.5,F1.0,F1.5,and F2.0)were successfully isolated from fucoidan.Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides,consisting mainly of mannuronic acid,fucose,and xylose,while the other fractions were predominantly composed of fucose and galactose.The sulfate group content increased from 8.74% to 18.76%,while the uronic acid content decreased from 23.79% to 5.58%across the fractions.All fractions of fucoidan exhibited significant antioxidant activity in various tests.There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals.Additionally,both AOS and the fucoidan fractions showed immunostimulation activity and were nontoxic to RAW264.7 cells.These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds.It has potential applications in the processing industry of brown seaweeds.展开更多
基金Supported by the Jiangsu Coast Development Group Co.,Ltd.the Marine Biological High Value Development and Utilization Project(No.2023YHTZZZ04)+1 种基金the Natural Science Foundation of Shandong Province(No.ZR2022MD007)the Science and Technology Project of Fujian Province(Nos.2022T3024,2023T3057)。
文摘Alginate oligosaccharides(AOS)and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds.We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from Lessonia trabeculata,and found that the optimum extraction procedure was:first,using 10-g/L Na_(2)CO_(3) solution to digest the seaweeds for 2 h at 80℃ with the material-liquid ratio 1:20(g/mL),and then adding 4.0%fucoidan cleavage enzyme for reaction for 12 h at 45℃.The yield of AOS and fucoidan was 21.36%and 14.40%,respectively,which was higher than those using traditional methods.The degree of polymerization of AOS was determined to be 3-5,confirming the results through thin layer chromatography(TLC)and electrospray ionization mass spectrometry(ESI-MS).The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97.Through anion-exchange column chromatography,four sulfated polysaccharide fractions(F0.5,F1.0,F1.5,and F2.0)were successfully isolated from fucoidan.Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides,consisting mainly of mannuronic acid,fucose,and xylose,while the other fractions were predominantly composed of fucose and galactose.The sulfate group content increased from 8.74% to 18.76%,while the uronic acid content decreased from 23.79% to 5.58%across the fractions.All fractions of fucoidan exhibited significant antioxidant activity in various tests.There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals.Additionally,both AOS and the fucoidan fractions showed immunostimulation activity and were nontoxic to RAW264.7 cells.These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds.It has potential applications in the processing industry of brown seaweeds.
