Effects of Different Seed Stem Sizes on the Changes of Available Elements in Rhizosphere Soil of Fritillaria thunbergii Miq.

[Objectives]This study was conducted to screen suitable seed stems of Fritillaria thunbergii Miq.from different provenances and to provide a theoretical basis for the high-yielding and high-efficiency cultivation of F.thunbergii Miq.introduced to different places.[Methods]F.thunbergii Miq.from four different provenances including Zhejiang,Nantong and Chongqing were introduced and cultivated in Wanzhou of Chongqing.The contents of available Zn,Fe,Mn,Cu,Mo,N,P,K,Ca and Mg in rhizosphere soil of F.thunbergii Miq.during five growing stages were determined after selecting different stem sizes for field cultivation.[Results]Small stems of Pan an and Ningbo provenances(SSG3,121-160/kg)and middle stems of Nantong and Fengjie provenances(SSG2,81-120/kg)showed higher soil availability.[Conclusions]In the process of introduction and cultivation of F.thunbergii Miq.,high yield and high efficiency can be achieved by selecting smaller seed stems of F.thunbergii Miq.

Preparation and Properties of Spray Polyurea Elastomer for Aquaculture Foam Floating Balls

[Objectives] This study was conducted to develop a polyurea elastomer which can be sprayed on the surface of expanded polystyrene (EPS) floating balls, so as to improve the surface strength and service life of the floating balls. [Methods] The effects of the types and amounts of isocyanate, chain extenders and polyether polyols on the gelation rate, adhesion and wear resistance of polyurea elastomer were investigated, and it was finally determined the preparation process of polyurea elastomer using liquid isophorone diisocyanate (IPDI) and amino-terminated polyether (D2000) as the main raw materials, dimethylthiotoluene diamine (E300) as the chain extender and silica as the wear resistance modifier through two-step solution polymerization of prepolymerization and chain extension. [Results] The physical properties and chemical resistance tests of spray polyurea elastomer showed that it had good physical properties and acid and alkali resistance, and could meet the requirements of spraying and protection of EPS floating ball surface in marine environment. [Conclusions] Polyurea elastomer coating can improve the aging resistance, wear resistance and acid and alkali resistance of EPS floating balls, and prevent them from being fragile and floating randomly to form marine floating garbage which results in "white pollution".

Visual Analysis of Application and Development Trend of Agarwood Based on CiteSpace

The Chinese traditional medicine,agarwood,is a commonly used medicine for regulating qi,which has many clinical applications because of its unique curative effect.In this study,CiteSpace software was used to visually analyze the application and development trend of agarwood in the literature from CNKI website in the period of 2007-2022.The analysis results showed that the research on agarwood has basically formed core groups of authors,and universities and their affiliated hospitals are main publishing institutions.Moreover,in this study,several research directions of agarwood were also summarized,including clinical research,chemical composition,structural identification and quality standards,showing that agarwood has rich and flexible application prospects in many aspects.On this basis,several suggestions were put forward:strengthening the cooperation between universities and research institutes and building a scientific research cooperation community,and promoting the combination of clinical research and laboratory research.

Visual Analysis of the Application and Development Trend of Cinnamon in the Field of Traditional Chinese Medicine Based on CiteSpace

[Objectives]This study was conducted to explore the application and development trend of Chinese medicinal material cinnamon in the field of traditional Chinese medicine.[Methods]Articles published from 2008 to 2023 were exported using"cinnamon"as the subject word in the Chinese database of CNKI.Knowledge graphs were drawn by CiteSpace software on the number of articles published,the institutions publishing articles,and keyword clustering,and the data were sorted by Excel.Combined with the extracted information,the application of cinnamon in traditional Chinese medicine and integrated traditional Chinese and Western medicine was analyzed,and its development trend was discussed and prospected,providing further reference for researchers.[Results]The number of articles published showed an overall upward trend and maintained a high number of articles.In the analysis of the journals publishing articles,the journal with the largest number of articles was West China Journal of Pharmaceutical Sciences,which had certain representativeness.In the analysis of institutions publishing articles,the institution with the largest number of articles was Beijing University of Chinese Medicine,and most institutions had little cooperation.Four categories was obtained in keyword clustering,respectively,general research,component identification,production process and product development of cinnamon."Glycyrrhizin"was the keyword with the earliest burst time,and the hot words that have received much attention in recent years are"medication law"and"data mining".[Conclusions]The application of cinnamon in the field of traditional Chinese medicine is mainly to treat diseases and as raw materials for traditional Chinese medicine products.The development trend is"quality control"and"product research and development".Further research and development of cinnamon in traditional Chinese medicine need to promote the participation of more institutions to participate,and cooperation and communication between institutions should be strengthened to promote the deep integration of production,research and academia.

The Role of Jasmonates as Antibulbing Substances in the Bulb Formation of Onion

Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extracts as an antibulbing substance,the amount of which was synchronized with the bulb formation.Since allene oxide synthase inhibitor canceled the antibulbing activity ofα-linolenic acid,it was disclosed that jasmonic acid concerns this regulation.Structure-activity-relationship study revealed that its(3R,7S)stereochemistry is necessary for showing its antibulbing activity.It is concluded that(3R,7S)-jasmonate derived fromα-linolenic acid actually participates in the regulation of bulb formation.

Effects of Abscisic Acid on Cold Resistance in Digitaria sanguinalis(L.)Scop.

[Objectives]This study was conducted to detect the protective effect of abscisic acid on chilling injury of Digitaria sanguinalis(L.)Scop,and whether this effect is related to antioxidant enzymes and osmotic adjustment.[Methods]D.sanguinalis plants were sprayed with abscisic acid solution,and exposed to cold stress at 15℃for 3 d after one day and then at 5℃for 25 to 30 d in a growth chamber.The changes of plant osmotic potential under this treatment were detected.[Results]Under low temperature stress,the osmotic potential of plants in the abscisic acid treatment and the control increased,but the osmotic potential level of the abscisic acid treatment plants was lower.The SOD activity of plants in the ABA treatment and the control decreased under low temperature stress.Under low temperature stress,the activity of catalase and peroxidase in ABA-treated plants was higher than that in control plants.[Conclusions]This study provides a theoretical basis for the impact of abscisic acid on the physiological response of D.sanguinalis to cold injury.

Effects of Functional Organic Materials on Fresh Weight,Quality,Single-melon Weight and SPAD Value of Watermelon

[Objectives]This study was conducted to explore a functional organic material formula suitable for watermelon cultivation with high quality,high yield and high efficiency.[Methods]Four treatments were set in the experiment,namely four functional organic materials,garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),to investigate the effects of different functional organic materials on fresh weight,quality,single-melon weight and SPAD value of watermelon.[Results]The effects of different functional organic materials on fresh weight,quality,single-melon weight and SPAD value of watermelon were quite different.The fresh weight,quality,single-melon weight and SPAD value of watermelon were higher in treatment T_(3)applying garlic straw and sheep manure and treatment T_(4)applying onion straw and chicken manure than in treatment T_(1)applying garlic straw and treatment T_(2)applying onion straw.Specifically,the fresh weight of whole plant was the highest in treatment T_(3),followed by treatment T_(4),and the values of the two treatments increased by 12.83%and 5.94%respectively compared with treatment T_(1);the weight of single melon was the highest in treatment T_(3),followed by treatment T_(4),and the values of the two treatments increased by 42.45%and 31.77%respectively compared with treatment T_(2);and the SPAD values of treatments T_(3)and T_(4)were significantly higher than those of treatments T_(1)and T_(2),and the value of treatment T_(3)was the largest.[Conclusions]This study provides theoretical support for the popularization and application of fertilization techniques combining organic fertilizers and reduced chemical fertilizers for watermelon.

Target-induced Trivalent G-quadruplex/hemin DNAzyme for Colorimetric Detection of Hg^(2+) Based on an Exonuclease III Assisted Catalytic Hairpin Assembly

Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type.

Effects of Functional Organic Materials on Morphological Indexes,Quality and Yield of Watermelon

[Objectives]This study was conducted to explore suitable organic compound application models for watermelon growth.[Methods]With watermelon hybrid material"M22×P18"as the test material,the effects of four functional organic materials,namely garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),on the morphological indexes,yield and quality of watermelon were investigated.[Results]Different functional organic materials had different effects on morphological indexes,yield and quality of watermelon.The morphological indexes,nutritional quality indexes and yield of watermelon treated with garlic straw and sheep manure compound(T_(3))and onion straw and chicken manure compound(T_(4))were significantly higher than those treated simply with garlic straw(T_(1))and onion straw(T_(2)),and T_(3)performed relatively better.Compared with treatment T_(2),T_(3)showed a stem diameter,vine length and leaf number increasing by 43.05%,46.69%and 40.77%respectively,central sugar and side sugar contents increasing by 11.72%and 21.90%respectively,and a yield increasing by 42.91%,with significant differences from T_(2).[Conclusions]This study provides technical support for high-quality and high-yielding cultivation of watermelon.

Research Progress of Necroptosis in Digestive Diseases

Digestive system diseases refer to organic and functional disorders of the digestive system,which are prone to recurrence and frequently accompanied by multiple complications.Necroptosis is a regulated mode of cell death mediated by death receptors,dependent on receptor protein activation,and could be specifically inhibited by necrostatin-1.Necroptosis is involved in pathological and physiological processes of various diseases,and plays an important role in the growth and development of organisms and the homeostasis of organ tissues.This paper reviewed the research advancement of necroptosis in digestive system disorders,and discussed the relationship between necroptosis and digestive system diseases,aiming to provide theoretical basis for the cure of these diseases.

Soaking Rice Seeds in High Concentration Salt Solution Improved Salt Tolerance at the Seedling Stage

[Objectives]This study was conducted to enhance the salt tolerance of current rice varieties at the seedling stage and fulfill the urgent requirement for salt-tolerant rice varieties in coastal tidal flats.[Methods]Four high-generation stable rice lines with diverse salt tolerance were employed as test materials,and four NaCl concentration gradients were established for seed soaking treatment.[Results]The seedling survival rate of line 151465 underwent significant alterations after soaking with four different salt concentrations,and the survival rate was the highest after treatment with 1.8%NaCl for 1 d,reaching 65.2%.The average survival rate of other three lines with different salt tolerance reached 62%after soaking with 1.8%NaCl for 1 d,which was significantly higher than those of the 2.2%NaCl and 0%NaCl treatments.[Conclusions]This study provides a basis for reducing the effect of abiotic stress on rice growth and development and improving the utilization rate of saline-alkali land.

Establishment of Double-antigen Sandwich Time-resolved Fluorescence Immunoassay for Detection of Pest des Petits Ruminants Virus

[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.

Acute Single and Joint Toxicity Effects of Deltamethrin and Lambda-cyhalothrin on Zebrafish(Danio rerio)

In this research,the single and combined toxicity effects of two commonly used pesticides,lambda-cyhalothrin and deltamethrin,were investigated on zebrafish at 20℃,with a weight of(1±0.1)g and a length of(3.5±0.35)cm.The study revealed that lambda-cyhalothrin exhibited higher toxicity compared to deltamethrin.Additionally,when used together,these pesticides showed significantly increased toxic effects on zebrafish.The 96-h LC 50 values were determined to be 3.059μg/L(confidence limits 0.077-0.351μg/L)for lambda-cyhalothrin and 1.304μg/L(confidence limits;0.046-0.228μg/L)for deltamethrin,both demonstrating a significant positive correlation(P<0.05).These results underscore the importance of regulating and managing pesticide use to safeguard aquatic organisms and uphold environmental sustainability.

Study on Antioxidant Enzyme Activity and Physiology and Biochemistry of Solanum nigrum L. under Glyphosate Stress

[Objectives] This study was conducted to investigate the scientific prevention and control of Solanum nigrum L. [Methods] Through experiments on S. nigrum from different sources, it was found that glyphosate stress had significant effects on antioxidant enzyme activity and oxidative damage of sensitive S. nigrum plants. [Results] Sensitive S. nigrum showed oxidative damage under glyphosate stress, while resistant S. nigrum responded to adversity damage by improving its antioxidant enzyme activity. The experimental results showed that the antioxidant enzymes and reduced glutathione of S. nigrum had certain metabolic detoxification effects under glyphosate stress. [Conclusions] This study provides a theoretical basis for scientific prevention and control of S. nigrum , and has a certain reference value for revealing the glyphosate resistance mechanism of S. nigrum .

Study on the Medication Rules of Traditional Chinese Medicine Prescriptions for the Treatment of New Crown Pneumonia Based on Data Mining Technology

[Objectives]This study was conducted to analyze the medication rules of clinical prescriptions of traditional Chinese medicine decoction pieces for the treatment of novel coronavirus pneumonia(COVID-19)during the epidemic in multiple regions based on data mining technology,so as to provide a reference for the treatment of COVID-19 with traditional Chinese medicine.[Methods]The traditional Chinese medicine prescriptions used since the outbreak of COVID-19 in Hubei Province during the fight against the epidemic from February 25,2020 to February 14,2022,the traditional Chinese medicine prescriptions used by Guizhou traditional Chinese medicine expert team aiding Hubei Province,the traditional Chinese medicine prescriptions for rehabilitation and conditioning of patients in Ezhou of Hubei Province after discharge,the traditional Chinese medicine prescriptions for the prevention and treatment of COVID-19 in Guizhou Province,and the traditional Chinese medicine prescriptions for the treatment of COVID-19 collected from the end of 2019 to the present from the Chinese database of CNKI were collected as the data of this study.Excel was used to establish a database and enter it into the TCM inheritance calculation platform V3.5,and the association rules and k-means clustering algorithm were used to analyze the frequency of herbal medicines in prescriptions during the treatment of COVID-19,the frequency of four natures,five flavors,meridian distribution,and drug combinations.[Results]A total of 1859 COVID-19 patients treated with traditional Chinese medicine were included,and the proportion of males was higher than that of females,and middle-aged and elderly people were the most common group.A total of 2170 prescriptions of traditional Chinese medicine were included,involving a total of 383 traditional Chinese medicines.High-frequency medicines included poria,Radix Bupleuri,Radix Scutellariae,Herba Pogostemonis,Fructus Forsythiae,Flos Loniceraeetc.The four natures were mainly concentrated in cold,warm and neutral,and the five flavors were mainly concentrated in bitter,pungent and sweet.The herbal medicines were mainly attributed to the lungs and stomach meridians,and were mainly of heat-clearing,exterior syndrome-relieving and diuresis-promoting and damp-clearing types.A total of 24 high-frequency herbal combinations and 35 association rule were excavated,and 3 types of formulas were obtained by cluster analysis.[Conclusions]The analysis results and medicine combinations obtained in the formulas are consistent with the traditional Chinese medicine treatment theory of COVID-19 caused by wind-heat filth accompanied with damp and toxin.

Proteomic Analysis of Stichopus japonicus and Evaluation of the Antiaging Properties of Its Peptide

The sea cucumber Stichopus japonicus is one of the"Eight Treasures of Seafood"and contains a number of bioactive components involved in multiple physiological and pharmacological functions.Proteins and peptides are generally considered to be responsible for these beneficial properties.In this study,a total of 3478 proteins and 17390 peptides were identified in Stichopus japonicus by proteomics methods.Among them,4 proteins were involved in 8 metabolic pathways,especially oxidative phosphorylation and cell senescence.Subsequently,lifespan assay and oxidative stress test were performed to investigate the peptides prepared from sea cucumber protein hydrolyzate using the aging model of Caenorhabditis elegans.The results of the anti-aging experiment demonstrated that high-dose peptides significantly prolonged the lifespan of nematodes(30.50%),and improved their capacity to inhibit oxidative stress.The results provide evidence supporting the development of bioactive proteins and peptides derived from Stichopus japonicus as functional foods and lay the foundation for the research of an anti-aging drug.

Breeding and Seed Reproduction Techniques of a New Tetraploid Common Buckwheat Variety‘Pintianqiao 3’

[Objectives]This study was conducted to actively carry out the breeding of new tetraploid common buckwheat varieties and its supporting breeding techniques.[Methods]Pintianqiao 3 is a new tetraploid common buckwheat variety developed by College of Agriculture of Shanxi Agricultural University and Agricultural Genetic Resources Center of Shanxi Agricultural University,using‘Pintianqiao 1’as the parent,through mutation treatment with 0.2%colchicine aqueous solution,grain selection,plant selection,isolation and identification,variety comparison,regional test and field investigation.The variety has chromosomes 2n=4X=32,and shows a spring sowing period of 101 d and a summer sowing period of 80 d,large flowers and seeds(with a 1000-grain weight of 41.4 g),and good resistance to lodging.[Results]From 2021 to 2022,Pintianqiao 3 participated in the independent joint regional test of common buckwheat varieties in Shanxi Province,and the average yield in 10 test positions was 1.8 kg,equivalent to 1800 kg/hm^(2),which was 8.4%higher than the control.It passed the field investigation conducted by Shanxi provincial expert group for identification of non-major crop varieties in Dongyang and Kelan experimental sites on September 2-3,2022.On January 4,2024,it passed the preliminary examination of Shanxi Provincial Crop Variety Approval Committee.The seed reproduction technique of Pintianqiao 3 including land selection,preparation before sowing,sowing,field management and timely harvesting has been developed.[Conclusions]This study provides technical support for the demonstration and popularization of this new variety.

Study on Effects of Curing Process on Characteristics of Marinated Beef Products

[Objectives]This study was conducted to compare the effects of different curing processes on the characteristics of marinated beef.[Methods]Marinated beef was obtained by two curing processes:static curing and injection and vacuum tumbling curing.The effects of the two curing processes on the production rate,curing absorption rate,water content,soluble protein content,amino acid nitrogen content,texture characteristics and microstructure of the product were compared.[Results]Compared with static curing,the production rate of marinated beef increased by 10%,the curing absorption rate increased by 28%,the texture and microstructure were improved,and the water content increased,while the soluble protein content decreased.As a result,the sensory score was higher.There was no significant difference in the content of amino acid nitrogen,but it decreased compared with raw meat.To sum up,injection and vacuum tumbling curing is more conducive to the processing of marinated beef.[Conclusions]This study provides a theoretical basis for the industrial production of marinated beef,and lays a foundation for in-depth exploration of injection and vacuum tumbling curing technique of marinated beef.

Study on Quality Standards of Jianjin Zhuanggu Paste

[Objectives]Some Chinese medicinal materials of Jianjin Zhuanggu Paste were microscopically identified,and several active ingredients were studied by thin-layer identification,which provides reference for further improving the quality standards of hospital preparations.[Methods]The effective components of Jianjin Zhuanggu Paste were qualitatively identified by thin-layer chromatography(TLC).[Results]The microscopic identification of the three Chinese medicinal materials in Jianjin Zhuanggu Paste showed the microscopic characteristics of Radix Codonopsis,Radix Astragali and Radix Notoginseng.TLC identification showed that there were characteristic spots of Radix Codonopsis,Radix Astragali,Radix Rehmanniae Preparata and Radix Notoginseng in Jianjin Zhuanggu Paste.[Conclusions]This study established the quality standard research method of Jianjin Zhuanggu Paste,which further strengthens the safety standards of hospital preparations,and improves the clinical efficacy of drugs,as well as the quality standards of hospital preparations to a certain extent.

Exploration on the Integration of Curriculum Ideological and Political Concept into the Teaching Design of"Culinary Raw Materials":A Case Study of Livestock and Poultry Raw Materials

Comprehensively promoting curriculum ideological and political education is a strategic measure to implement the fundamental task of establishing moral integrity and educating people.Culinary raw materials is the core curriculum of culinary majors,which is rich in ideological and political elements.In this study,taking livestock and poultry raw materials as an example,the teaching design of integrating curriculum ideological and political education into culinary raw materials were investigated by sorting out its teaching contents and excavating its ideological and political elements.Taking livestock and poultry raw materials in Teochew Cuisine Raw Materials as an example,the teaching design of curriculum ideological and political education was conducted,aiming to provide some reference and guidance for curriculum ideological and political education of the curriculum.