单侧列序样排列的家族性良性慢性天疱疮1例

患者左上肢皮损呈线状与肢体长轴平行，左躯干皮损沿肋间呈区带状分布，临床表现酷似线状痣。但患者皮损以水疱为主，病理检查缺乏线状痣的组织学特点，而符合家族性良性慢性天疱疮的诊断。

列序聚类法和最小变化拟合在体育选材中的应用

本文应用列序聚类法和最小变化拟合于运动员选材。介绍了这种方法的基本步骤,给出了计算程序和得到解的迭代过程。结果表明,这种方法在运动员选材中是非常有效的。

列序分析在大气环境污染分析中的应用

提出了不同于计算相关系数的列序分析的方法,思路来源于变量序列间的几何形状接近程度,几何形状接近的序列,度量其靠近程度的指数应大,反之就小。将靠近程度大小按序排列,就得到变量序列间的联系程度的名次。从实用出发,针对不同性质的数据,提出了4种列序度计算方案。选取了计算加权平方距离的方案对大气污染及其有关的气象要素数据进行了实例计算。通过计算列序度并与相关系数对照,可以更可靠地来使用某个气象要素制作预报,另外,从数学上证明列序分析与绝对值关联度、欧氏距离间的关系,由此论证了列序分析的数学根基。数学推导和数值计算都证实了列序分析的可用性,尤其在量测为小样本的情况下更有使用价值。

列序压缩算子的不动点和Hammerstein型积分方程

给出了Banach空间列序压缩算子定义,讨论了此类算子不动点存在性问题和其在非线性H amm erste in型积分方程中的应用.

列序分析及其对空气污染的应用

在数据分析中常用计算相关系数来分析因变量与自变量间的关系,其基本概念是以统计样本来对总体进行估计.列序分析则宁可把问题限制在样本集内,即在已有采样数据的情况下,因变量与m个自变量中哪个自变量联系最密切?哪个又居其次?应该说列序分析更切合实际需要.论证了列序分析的原理、数学表达,同时用大气污染的实测数据进行了计算,对结果进行了分析,且与相关系数的异同作了对比.

杜甫献赋仅得“参列选序”的原因

从唐代官吏铨选制度这一角度出发，分析杜甫献三大礼赋之后仅得“参列选序”资格的前后过程，考察出其根本原因在于唐代的守选制。

格序模f-张量积函子的正合性与平坦格序模

本文证明了如下结果:设M是有单位元可换格序环R上的格序模和l-模同态范畴,F是R上的f-模和l-模同态范畴,M∈M,则F=M(?)()是M 到F 的共变函子,进而是右正合函子.设R 是有单位元的可换全序环,视R 为自身上的格序模,则R 是平坦的.

从序时词语的基准看动意词语的着眼点

动词本身就具有时量属性 ,基准可以显现动词时量属性实现的语法、语义的规则。对“动—序组列”格式中动意词语作基准的着眼点的种类的分析 ,不仅可以揭示着眼点造成的歧义 ,而且有助于揭示动意词语的非基准用法 ,这除了说明动作有不同的着眼点外 ,还充实了起始点逆向漂移的类型 ,以此增强语用的解释力 。

Two new species of the genus Bifidocoelotes from southern China,with the first description of the male of Bifidocoelotes elongatus(Agelenidae,Coelotinae)

Two new species of the genus Bifidocoelotes Wang,2002 are described from Guangxi Zhuang Autonomous Region of China:B.alviolatus sp.nov.(♂♀),B.latus sp.nov.(♀),and the male of B.elongatus Liao,Wang,Yin&Xu,2022 is described for the first time.Detailed descriptions,morphological illustrations and a distribution map of Bifidocoelotes are provided.DNA barcodes of four Bifidocoelotes species are also provided,including the new species(B.alviolatus sp.nov.)and three known species(B.mammiformis Liao et al.,2022;B.quadratus Liao et al.,2022;B.elongatus Liao et al.,2022).

An Improved Framework of Massive Superpoly Recovery in Cube Attacks Against NFSR-Based Stream Ciphers

A critical problem in the cube attack is how to recover superpolies efficiently.As the targeting number of rounds of an iterative stream cipher increases,the scale of its superpolies becomes larger and larger.Recently,to recover massive superpolies,the nested monomial prediction technique,the algorithm based on the divide-and-conquer strategy,and stretching cube attacks were proposed,which have been used to recover a superpoly with over ten million monomials for the NFSR-based stream ciphers such as Trivium and Grain-128AEAD.Nevertheless,when these methods are used to recover superpolies,many invalid calculations are performed,which makes recovering superpolies more difficult.This study finds an interesting observation that can be used to improve the above methods.Based on the observation,a new method is proposed to avoid a part of invalid calculations during the process of recovering superpolies.Then,the new method is applied to the nested monomial prediction technique and an improved superpoly recovery framework is presented.To verify the effectiveness of the proposed scheme,the improved framework is applied to 844-and 846-round Trivium and the exact ANFs of the superpolies is obtained with over one hundred million monomials,showing the improved superpoly recovery technique is powerful.Besides,extensive experiments on other scaled-down variants of NFSR-based stream ciphers show that the proposed scheme indeed could be more efficient on the superpoly recovery against NFSR-based stream ciphers.

The Applications of CRISPR Screening in Aging Research

The advancement of Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)gene editing technology has revolutionized the comprehension of human genome,propelling molecular and cellular biology research into unexplored realms and accelerating progress in life sciences and medicine.CRISPR-based gene screening,recognized for its efficiency and practicality,is widely utilized across diverse biological fields.Aging is a multifaceted process governed by a myriad of genetic and epigenetic factors.Unraveling the genes regulating aging holds promise for understanding this intricate phenomenon and devising strategies for its assessment and intervention.This review provides a comprehensive overview of the progress in CRISPR screening and its applications in aging research,while also offering insights into future directions.CRISPR-based genetic-manipulation tools are positioned as indispensable instruments for mitigating aging and managing age-related diseases.

Multi-dimension and multi-modal rolling mill vibration prediction model based on multi-level network fusion

Mill vibration is a common problem in rolling production,which directly affects the thickness accuracy of the strip and may even lead to strip fracture accidents in serious cases.The existing vibration prediction models do not consider the features contained in the data,resulting in limited improvement of model accuracy.To address these challenges,this paper proposes a multi-dimensional multi-modal cold rolling vibration time series prediction model(MDMMVPM)based on the deep fusion of multi-level networks.In the model,the long-term and short-term modal features of multi-dimensional data are considered,and the appropriate prediction algorithms are selected for different data features.Based on the established prediction model,the effects of tension and rolling force on mill vibration are analyzed.Taking the 5th stand of a cold mill in a steel mill as the research object,the innovative model is applied to predict the mill vibration for the first time.The experimental results show that the correlation coefficient(R^(2))of the model proposed in this paper is 92.5%,and the root-mean-square error(RMSE)is 0.0011,which significantly improves the modeling accuracy compared with the existing models.The proposed model is also suitable for the hot rolling process,which provides a new method for the prediction of strip rolling vibration.

Analysis of Population Structure and Genetic Diversity of Authentic Medicinal Herb Dendrobium huoshanense Using ESTSSR Markers

Understanding the intra-population genetic diversity and population genetic structure is beneficial for plant protection and breeding.Dendrobium huoshanense is an endangered authentic medicinal herb.To analyze the population differentiation and genetic diversity in D.huoshanense,simple sequence repeat(SSR)markers were obtained by high-throughput transcriptome sequencing,and 10 expressed sequence tag(EST)-SSRs were studied in 182 Dendrobium individual samples from 15 populations distributed in Huoshan County,southwest Anhui Province.After deep sequencing of D.huoshanense transcriptome,a total of 58202 unigenes were obtained,and 7781 SSRs with 1~6 base repeats were detected.Among them,dinucleotide SSRs were the dominant repeating motifs(3119,40.085%),and the most common was AG/CT(2666,54.01%).Ten SSR primers were screened out from the designed 120 primer pairs for further polymorphism verification.The results showed that 75 alleles were detected,and the number of alleles at each locus ranged from 4 to 11,with an average of 7.5.The mean expected heterozygosity(H_(e)),observed heterozygosity(H_(o))and polymorphism information content(C_(pi))of the 10 SSR loci were 0.646,0.574,and 0.607,respectively.D.huoshanense populations exhibited a high level of genetic diversity(H_(o)=0.591,H_(e)=0.557)and a moderate level of differentiation(F_(ST)=0.10069),and the genetic variation mainly existed within populations.The results of Structure and neighbour-joining(NJ)analysis based on 10 polymorphic primers showed that D.huoshanense populations were clustered into 2 groups,and the populations KS,HSD,and JXZ had rich genetic diversity and multiple private alleles,suggesting that these populations could be used for genetic improvement of the species.The study revealed frequency distribution of D.huoshanense SSR loci,and successfully developed microsatellite genetic markers,which are expected to expand the repertoire of DNA markers for genetic study of D.huoshanense.

ICPS multi-target constrained comprehensive security control based on DoS attacks energy grading detection and compensation

Aiming at the industry cyber-physical system(ICPS)where Denial-of-Service(DoS)attacks and actuator failure coexist,the integrated security control problem of ICPS under multi-objective constraints was studied.First,from the perspective of the defender,according to the differential impact of the system under DoS attacks of different energies,the DoS attacks energy grading detection standard was formulated,and the ICPS comprehensive security control framework was constructed.Secondly,a security transmission strategy based on event triggering was designed.Under the DoS attack energy classification detection mechanism,for large-energy attacks,the method based on time series analysis was considered to predict and compensate for lost data.Therefore,on the basis of passive and elastic response to small energy attacks,the active defense capability against DoS attacks was increased.Then by introducing the conecomplement linearization algorithm,the calculation methods of the state and fault estimation observer and the integrated safety controller were deduced,the goal of DoS attack active and passive hybrid intrusion tolerance and actuator failure active fault tolerance were realized.Finally,a simulation example of a four-capacity water tank system was given to verify the validity of the obtained conclusions.

移动信道差错序列的分布概率模拟法及门限电平的讨论

采用了一种新方法产生移动信道的差错序列。即用计算机产生(0,1)均匀分布伪随机数,通过公式变换,产生Rayleigh分布随机数,加以适当门限,使模拟量量化,形成差错序列{Ei}、统计无误串分布G(K),并与实测G(K)曲线比较分析。由计算机模拟结果可知:概率模拟法是一种行之有效的方法。通过门限电平的讨论,发现此门限电平具有自适应特性。

cpSSR: a New Tool to Analyze Chloroplast Genome of Citrus Somatic Hybrids

Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses.

A Preliminary Analysis of Phylogenetic Relationships in Cymbidium (Orchidaceae) Based on nrITS Sequence Data

The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27 species and 3 cultivars of Cymbidium and 3 outgroup species ( Eulophia graminea, Geodorum densiflorum, Amitostigma pinguiculum) were analyzed using PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis, however, show that the existing division among three subgenera (subgen. Cymbidium , subgen. Cyperorchis and subgen. Jensoa ) should be evaluated with more data. Subgenus Cyperorchis was not a monophyletic group, with the unexpected nesting of C. dayanum (subgen. Cymbidium ) within it; subgenus Jensoa also appeared paraphyletic, with C. lancifolium being the sister group to the remainder of the genus; species of subgen. Cymbidium appeared polyphyletic, being split into several clades and intermixed with the main subgen. Cyperorchis and subgen. Jensoa clades, respectively. However, because of the insufficiency of informative characters of ITS sequences, some of the clades identified, especially the major lineages of Cymbidium , received relatively low support; sectional delimitations were also not clear within each subgenus. Further study is needed for achieving a robust phylogeny of Cymbidium .

cDNA Cloning and Sequencing of Squalene Synthase of Artemisa apiacea

[Objective] cDNA from squalene synthase was cloned and sequenced.[Method] A pair of specific primers was designed according to the cDNA gene sequence of squalene synthase published in GenBank.Total RNA was extracted from the cell of Artemisia apiacea.The genes of squalene synthase were amplified by using RT-PCR.It was connected with pMD19-T vector and the cloned fragment sequences were analyzed.[Result] SS gene with the whole length of 1 257 bp was amplified and the fragment encoded 418 amino acids.The homo...

Identification of Stellaria media by PCR

Aim To provide a rapid and reliable method for identifying the fork medicine Stellaria media （Linn. ） Cyr. （Herba Stellariae mediae） （Caryophyllaceae） from its adulterant Myosoton aquaticure （L.） Fries （Herba Myosoti aquatici） （Caryophyllaceae） by polymerase chain reaction （PCR） technology. Methods A molecular genetic approach has been developed to identify S. media for the first time. 5S-rRNA spacer domain was amplified by PCR from the isolated genomic DNA, and the PCR products were then sequenced. Results The nucleotide sequences of S. media and M. aquaticum were measured to determine their identity. Furthermore, the nucleotide sequences of three Stellaria species, S. vestita, S. longifolia and S. radians, were also measured for the sake of providing the evidence of the biological phylogeny of SteUaria. Diversity between DNA sequence and restriction enzyme mapping among a variety of the species was found in their 5S-rRNA spacer domains. Conclusion The 5S-rRNA spacer domains can be used as a molecular marker for differentiating S. media from M. aquaticum and in phylogenetie studies of Stellaria.

Cloning and Analysis of NBS-LRR Type Resistance Gene Analogues in Sweet Potato (Ipomoea batatas)

The degenerate primers were designed based on the conserved NBS-LRR motifs among the known disease-resistance genes. A fragment of about 500 bp was amplified from genomic DNA of sweet potato using the specifically designed degenerate primers. After cloning and sequencing, 20 NBS-LRR type of disease-resistance gene analogue （RGAs） in sweet potato were observed. The deduced amino acid sequence of DNA fragment contains the conserved motifs of NBS-LRR type RGAs, such as P-loop, Kinase-2α, Kinase-3α and GLPL domain. The 20 RGAs could be sorted into two subclasses, namely TIR- NBS-LRR type and non-TIR-NBS-LRR type. Compared with the known resistance genes including N, L6 and M, the percentages of homologous amino acid sequence in 10 TIR-NBS-LRR range between 21% -44%. While other 10 non-TIR-NBS-LRR assume 15% -46% homology with the known resistance genes （Prf, RPM1, RPS2, etc. ）. Consequently the RGAs may further be used as molecular marker for screening the candidate disease-resistance genes in sweet potato.