With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene ...With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.展开更多
Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and nu...Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and number of effective alleles （Ae） were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package （version 3.63）. There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1） the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2） the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.展开更多
The objectives of this study were to isolate and characterize microsatellites from a heat tolerant variety of snap bean (Phaseolus vulgaris L.) in order to generate polymorphic genetic markers linked to quantitative t...The objectives of this study were to isolate and characterize microsatellites from a heat tolerant variety of snap bean (Phaseolus vulgaris L.) in order to generate polymorphic genetic markers linked to quantitative trait loci for heat tolerance. A genomic library contained 400-800 bp inserts was constructed and screened for the presence of (GA/CT) n and (CA/GT) n repeats. The proportion of positive clones yielded estimated of 3.72×10 4 such dinucleotide repeats per genome, roughly comparable to the abundance reported in other eukaryotic genomes. Twenty_six positive clones were sequenced. In contrast to mammalian genomes, the (GA/CT) n motif was much more abundant than the (CA/GT) n motif in these clones. The (GA/CT) n repeats also showed longer average repeat length (mean n =10.4 versus 6.5), suggesting that they are better candidates for yielding polymorphic genetic markers in the snap bean genome.展开更多
A stochastic approach,namely,Monte Carlo method with"tree structure pattern"has been adopted tosimulate the random backrnixing,residence time,reaction and dynamics of solid particles with size distribu-tion ...A stochastic approach,namely,Monte Carlo method with"tree structure pattern"has been adopted tosimulate the random backrnixing,residence time,reaction and dynamics of solid particles with size distribu-tion and with complex reaction in a complex flow system.Turbulence of particles in reactor system is simu-lated by stochastic tree pattern which is shown by pseudo-random number series picked from a uniformlydistributed set of numbers between zero and one.A Monte Carlo procedure is employed to model the par-ticles’ stochastic residence time,random flow and reaction with fluid.A large number of discrete bundlesof particles have been simulated and followed.Statistic results of all particles in the last information representthe solution to the problem under steady state of flow.The approach has a simple physical concept and simulation procedure.All calculation has been carriedout easily with a computer.For simple reaction system,results are in good agreement with those obtainedfrom the known approach and experiments.The method could provide a new kind of technique for simu-lating the behavior of particles in a continuous flow reactor.展开更多
Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique...Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.展开更多
A new pseudolites （PLs） structure optimization model of global navigation satellite system （GNSS）/PLs integration positioning system used in deep open-pit mine was presented. Position dilution of precision （Pdop...A new pseudolites （PLs） structure optimization model of global navigation satellite system （GNSS）/PLs integration positioning system used in deep open-pit mine was presented. Position dilution of precision （Pdop） and reliability were selected as the optimization indicators to build a multi-objective optimization model to decide the optimum PLs location. A scheme was designed by establishing a four-dimensional model taking azimuth （a）, elevation angle （e） and epoch （t） of satellites as the input independent variables and Pdop as the dependent variable to calculate the optimum PLs location zone considering the real circumstances. And then the ultimate PLs location can be fixed by testing the curves of Pdop along time. A field collected Trimble R8 GPS data set in China University of Mining and Technology （CUMT） campus was used for the model test to show the effectiveness, and the proposed PLs optimum design scheme was used at the west open-pit mine of Fushun mining group Co., Ltd., in China, better Pdop and reliability have been achieved for the integration system. Both experiments show that the proposed scheme is excellent in designing GNSS/PLs system which is helpful for improving the performance of the positioning system and reducing the cost.展开更多
文摘With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.
基金This work is supported by the Key Project of Chinese National Program for Fundamental Research and Development (No. 2004CB- 117405)Chinese commonweal Program (No. 2005DIB4J024).
文摘Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and number of effective alleles （Ae） were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package （version 3.63）. There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1） the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2） the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.
文摘The objectives of this study were to isolate and characterize microsatellites from a heat tolerant variety of snap bean (Phaseolus vulgaris L.) in order to generate polymorphic genetic markers linked to quantitative trait loci for heat tolerance. A genomic library contained 400-800 bp inserts was constructed and screened for the presence of (GA/CT) n and (CA/GT) n repeats. The proportion of positive clones yielded estimated of 3.72×10 4 such dinucleotide repeats per genome, roughly comparable to the abundance reported in other eukaryotic genomes. Twenty_six positive clones were sequenced. In contrast to mammalian genomes, the (GA/CT) n motif was much more abundant than the (CA/GT) n motif in these clones. The (GA/CT) n repeats also showed longer average repeat length (mean n =10.4 versus 6.5), suggesting that they are better candidates for yielding polymorphic genetic markers in the snap bean genome.
文摘A stochastic approach,namely,Monte Carlo method with"tree structure pattern"has been adopted tosimulate the random backrnixing,residence time,reaction and dynamics of solid particles with size distribu-tion and with complex reaction in a complex flow system.Turbulence of particles in reactor system is simu-lated by stochastic tree pattern which is shown by pseudo-random number series picked from a uniformlydistributed set of numbers between zero and one.A Monte Carlo procedure is employed to model the par-ticles’ stochastic residence time,random flow and reaction with fluid.A large number of discrete bundlesof particles have been simulated and followed.Statistic results of all particles in the last information representthe solution to the problem under steady state of flow.The approach has a simple physical concept and simulation procedure.All calculation has been carriedout easily with a computer.For simple reaction system,results are in good agreement with those obtainedfrom the known approach and experiments.The method could provide a new kind of technique for simu-lating the behavior of particles in a continuous flow reactor.
基金Supported by Technology Innovation Projects from Science and Tech-nology Department of Guangdong Province (2008A023700011)~~
文摘Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.
基金Project(2013RC16)supported by the Fundamental Research Funds for the Central Universities,China
文摘A new pseudolites （PLs） structure optimization model of global navigation satellite system （GNSS）/PLs integration positioning system used in deep open-pit mine was presented. Position dilution of precision （Pdop） and reliability were selected as the optimization indicators to build a multi-objective optimization model to decide the optimum PLs location. A scheme was designed by establishing a four-dimensional model taking azimuth （a）, elevation angle （e） and epoch （t） of satellites as the input independent variables and Pdop as the dependent variable to calculate the optimum PLs location zone considering the real circumstances. And then the ultimate PLs location can be fixed by testing the curves of Pdop along time. A field collected Trimble R8 GPS data set in China University of Mining and Technology （CUMT） campus was used for the model test to show the effectiveness, and the proposed PLs optimum design scheme was used at the west open-pit mine of Fushun mining group Co., Ltd., in China, better Pdop and reliability have been achieved for the integration system. Both experiments show that the proposed scheme is excellent in designing GNSS/PLs system which is helpful for improving the performance of the positioning system and reducing the cost.
