Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of...Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.展开更多
Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolat...Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolate(WMV-Ch)was firstly determined.Excluding the 3’-terminal poly(A)tail,the genome was 10037 nt long,containing 5’-and 3’-terminal untranslated regions and a large open reading frame.The genome encoded a polyprotein of 3217 amino acids with a predicted Mr of 365.8 kD.Based on comparison with the proposed location of cleavage sites of other potyvirus polyproteins,10 mature proteins were predicted.There were 92.5% nucleotide and 96.4% amino acid identities over entire genome between WMV-Ch and France isolate(WMV-Fr),respectively.The nucleotide identity of the coat protein genes among WMV-Ch and WMV from different countries was all less than 95%,and phylogenetic tree revealed that WMV-Ch was most close to Japanese isolate.展开更多
文摘Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.
文摘Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolate(WMV-Ch)was firstly determined.Excluding the 3’-terminal poly(A)tail,the genome was 10037 nt long,containing 5’-and 3’-terminal untranslated regions and a large open reading frame.The genome encoded a polyprotein of 3217 amino acids with a predicted Mr of 365.8 kD.Based on comparison with the proposed location of cleavage sites of other potyvirus polyproteins,10 mature proteins were predicted.There were 92.5% nucleotide and 96.4% amino acid identities over entire genome between WMV-Ch and France isolate(WMV-Fr),respectively.The nucleotide identity of the coat protein genes among WMV-Ch and WMV from different countries was all less than 95%,and phylogenetic tree revealed that WMV-Ch was most close to Japanese isolate.
