目的了解内蒙古自治区部分地区鸡蛋中土霉素和强力霉素残留情况,并评估膳食风险。方法在内蒙古自治区部分地区的农贸市场、商店、网店等采集共180份样品,采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS...目的了解内蒙古自治区部分地区鸡蛋中土霉素和强力霉素残留情况,并评估膳食风险。方法在内蒙古自治区部分地区的农贸市场、商店、网店等采集共180份样品,采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)进行检测,分析鸡蛋中的土霉素、强力霉素残留含量,根据内蒙古居民的饮食消费情况,采用点评估法和食品安全指数法对鸡蛋中兽药的暴露风险进行评估。结果2022—2023年内蒙古自治区部分地区土霉素、强力霉素总体样本检出率为24.44%(44/180),超标率为12.22%(22/180)。强力霉素检出42份,检出率为23.33%(42/180),超标率为12.22%(22/180);土霉素检出3份,检出率为1.67%(3/180),此次调查中无超标样本;鸡蛋中强力霉素食品安全指数均值为0.001851,最大值为0.2137,均小于1。结论内蒙古自治区部分地区鸡蛋样本中存在少量的土霉素和强力霉素残留,暴露风险较低,对人体健康危害处在可接受范围。展开更多
鉴于四环素类抗生素大量使用造成的环境污染以及人体肝损伤、肠道功能紊乱和过敏等不良反应,亟需开发一种灵敏度高、操作简便、价格低廉的四环素类抗生素残留检测方法。荧光法具有操作简单、成本低廉、可视化等优点,因此,本工作基于MXen...鉴于四环素类抗生素大量使用造成的环境污染以及人体肝损伤、肠道功能紊乱和过敏等不良反应,亟需开发一种灵敏度高、操作简便、价格低廉的四环素类抗生素残留检测方法。荧光法具有操作简单、成本低廉、可视化等优点,因此,本工作基于MXene QDs和铕离子构建了可选择性检测强力霉素(DOX)的比率荧光探针。以尿酸和单层Ti3CN为原料通过水热法制备得MXene QDs,MXene QDs与Eu3+通过自组装构建得到MXene QDs-Eu3+双发射荧光探针,用于DOX的选择性检测。并通过优化探针体系中的MXene QDs浓度、Eu3+浓度、pH和响应时间等实验条件,探究该双发射荧光探针对强力霉素的定量和选择性检测性能。结果表明,该荧光探针对DOX具有特异性识别能力,且荧光强度比(F615/F421)与不同浓度DOX (0∼30 μM)之间存在良好的线性关系,表明其在TCs残留检测中具有良好应用前景。Given the environmental pollution, liver damage, intestinal dysfunction, and allergic reactions caused by the extensive use of tetracycline antibiotics, there is an urgent need to develop a sensitive, simple, and cost-effective method for detecting residual tetracycline antibiotics. The fluorescence method possesses advantages such as simple operation, low cost, and visualizability. Therefore, this work constructs a ratio fluorescence probe for the selective detection of doxycycline based on MXene QDs and rare earth ions. We prepared MXene QDs using uric acid and single-layer Ti3CN as raw materials through a hydrothermal method. The MXene QDs-Eu3+ dual-emission fluorescent probe was constructed by self-assembly of MXene QDs and Eu3+, which was used for the selective detection of streptomycin. By optimizing the experimental conditions such as the concentration of MXene QDs, Eu3+, pH, and response time in the probe system, this dual-emission fluorescent probe was explored for quantitative and selective detection of streptomycin. Findings show that the fluorescent probe uniquely identifies gentamicin, with a strong linear correlation between the fluorescence intensity ratio (F615/F421) and different gentamicin concentrations (0~30 μM), hinting at its potential use in identifying tetracycline residues.展开更多
文摘目的了解内蒙古自治区部分地区鸡蛋中土霉素和强力霉素残留情况,并评估膳食风险。方法在内蒙古自治区部分地区的农贸市场、商店、网店等采集共180份样品,采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)进行检测,分析鸡蛋中的土霉素、强力霉素残留含量,根据内蒙古居民的饮食消费情况,采用点评估法和食品安全指数法对鸡蛋中兽药的暴露风险进行评估。结果2022—2023年内蒙古自治区部分地区土霉素、强力霉素总体样本检出率为24.44%(44/180),超标率为12.22%(22/180)。强力霉素检出42份,检出率为23.33%(42/180),超标率为12.22%(22/180);土霉素检出3份,检出率为1.67%(3/180),此次调查中无超标样本;鸡蛋中强力霉素食品安全指数均值为0.001851,最大值为0.2137,均小于1。结论内蒙古自治区部分地区鸡蛋样本中存在少量的土霉素和强力霉素残留,暴露风险较低,对人体健康危害处在可接受范围。
文摘鉴于四环素类抗生素大量使用造成的环境污染以及人体肝损伤、肠道功能紊乱和过敏等不良反应,亟需开发一种灵敏度高、操作简便、价格低廉的四环素类抗生素残留检测方法。荧光法具有操作简单、成本低廉、可视化等优点,因此,本工作基于MXene QDs和铕离子构建了可选择性检测强力霉素(DOX)的比率荧光探针。以尿酸和单层Ti3CN为原料通过水热法制备得MXene QDs,MXene QDs与Eu3+通过自组装构建得到MXene QDs-Eu3+双发射荧光探针,用于DOX的选择性检测。并通过优化探针体系中的MXene QDs浓度、Eu3+浓度、pH和响应时间等实验条件,探究该双发射荧光探针对强力霉素的定量和选择性检测性能。结果表明,该荧光探针对DOX具有特异性识别能力,且荧光强度比(F615/F421)与不同浓度DOX (0∼30 μM)之间存在良好的线性关系,表明其在TCs残留检测中具有良好应用前景。Given the environmental pollution, liver damage, intestinal dysfunction, and allergic reactions caused by the extensive use of tetracycline antibiotics, there is an urgent need to develop a sensitive, simple, and cost-effective method for detecting residual tetracycline antibiotics. The fluorescence method possesses advantages such as simple operation, low cost, and visualizability. Therefore, this work constructs a ratio fluorescence probe for the selective detection of doxycycline based on MXene QDs and rare earth ions. We prepared MXene QDs using uric acid and single-layer Ti3CN as raw materials through a hydrothermal method. The MXene QDs-Eu3+ dual-emission fluorescent probe was constructed by self-assembly of MXene QDs and Eu3+, which was used for the selective detection of streptomycin. By optimizing the experimental conditions such as the concentration of MXene QDs, Eu3+, pH, and response time in the probe system, this dual-emission fluorescent probe was explored for quantitative and selective detection of streptomycin. Findings show that the fluorescent probe uniquely identifies gentamicin, with a strong linear correlation between the fluorescence intensity ratio (F615/F421) and different gentamicin concentrations (0~30 μM), hinting at its potential use in identifying tetracycline residues.
