Toxoplasmosis is an important cause of reproductive failure in man and farm animals resulting in significant socio-economic losses worldwide. The aim of this study was to avail large scale data-regarding the sero-prev...Toxoplasmosis is an important cause of reproductive failure in man and farm animals resulting in significant socio-economic losses worldwide. The aim of this study was to avail large scale data-regarding the sero-prevalence of T. gondii infection in dairy animals from the Khartoum State, Sudan using the commercially available Latex Agglutination Diagnostic kits. The study revealed that the overall seroprevalence of T. gondii infection at herd level of dairy animals in the State was 92.7%. The within herd seroprevalence was ranging from 8% up to 100% with mean of 51.3% in different herds of different dairy animals species in the State. Only 6 herds, 3 herds, 3 herds and 1 herd of cattle, camel sheep and goat respectively were found to be clean from anti-T, gondii antibody in the present study. Interestingly, most of the clean herds （12 herds） are located in the Khartoum district. The differences between the three districts, the seven localities and the four animal species were statistically highly significant （P 〈 0.01）. The overall sero-prevalence of T. gondii infection in dairy animals--at individual level--was 45.3%. Sheep scored the highest seroprevalence rate （75.0%） followed by goats （64.0%）, camels （54.1%） and cattle （40.9%） with high statistically significant differences （P 〈 0.01）. The highest level of antibody titration was reported in sheep and goats. This is the first area-wide and large scale report on seroprevalence of T. gondii infection in dairy animals from the Sudan. It was concluded that dairy animals in the Sudan are widely exposed to T. gondii and people in the Sudan should be aware of the possibility of hyper-prevalence of human toxoplasmosis through these food animals. Research on clinical toxoplasmosis in dairy animals and human in the Sudan is recommended to evaluate the role T. gondii infection in economical losses in dairy farm industry and for building strategy of sustainable toxoplasmosis control.展开更多
With the purpose to determine the frequency and type of cardiac lesions in naturally exposed dogs to Trypanosoma cruzi, ninety one stray dogs, capture by the Canine and Feline Control Center （dog pound） from the mun...With the purpose to determine the frequency and type of cardiac lesions in naturally exposed dogs to Trypanosoma cruzi, ninety one stray dogs, capture by the Canine and Feline Control Center （dog pound） from the municipality of Merida, were studied. Before euthanasia, blood samples were taken to detect 72 cruzi antigens by indirect immunofluorescence antibody test and Western Blot and to detect the genome of parasite by Polymerase Chain Reaction. Immediately after euthanasia, hearts were macroscopically evaluated and a sample of the middle right atrial wall of each dog was taken for histopathological analyses. DNA was also obtained from paraffin blocks of seropositives animals with microscopic lesions to detect 72 cruzi genome. Of ninety one dogs, thirteen were seropositive. All seropositive dogs showed an association （P 〈 0.05） with lymphocytoplasmatic myocarditis. The presence of the 72 cruzi genome was also detected by PCR in cardiac septum tissue of seropositive dogs and in all the cases with microscopic lesions indicating the high pathogenicity of the local circulating strain. No association with macroscopic lesions was observed in seropositive dogs. Also, the presence of Dirofilaria immitis （D. immitis） was found in 6% of dogs evaluated. This study demonstrates a high tropism to cardiac tissue and virulence of the strains of 72 cruzi circulating in the studied dog population.展开更多
文摘Toxoplasmosis is an important cause of reproductive failure in man and farm animals resulting in significant socio-economic losses worldwide. The aim of this study was to avail large scale data-regarding the sero-prevalence of T. gondii infection in dairy animals from the Khartoum State, Sudan using the commercially available Latex Agglutination Diagnostic kits. The study revealed that the overall seroprevalence of T. gondii infection at herd level of dairy animals in the State was 92.7%. The within herd seroprevalence was ranging from 8% up to 100% with mean of 51.3% in different herds of different dairy animals species in the State. Only 6 herds, 3 herds, 3 herds and 1 herd of cattle, camel sheep and goat respectively were found to be clean from anti-T, gondii antibody in the present study. Interestingly, most of the clean herds （12 herds） are located in the Khartoum district. The differences between the three districts, the seven localities and the four animal species were statistically highly significant （P 〈 0.01）. The overall sero-prevalence of T. gondii infection in dairy animals--at individual level--was 45.3%. Sheep scored the highest seroprevalence rate （75.0%） followed by goats （64.0%）, camels （54.1%） and cattle （40.9%） with high statistically significant differences （P 〈 0.01）. The highest level of antibody titration was reported in sheep and goats. This is the first area-wide and large scale report on seroprevalence of T. gondii infection in dairy animals from the Sudan. It was concluded that dairy animals in the Sudan are widely exposed to T. gondii and people in the Sudan should be aware of the possibility of hyper-prevalence of human toxoplasmosis through these food animals. Research on clinical toxoplasmosis in dairy animals and human in the Sudan is recommended to evaluate the role T. gondii infection in economical losses in dairy farm industry and for building strategy of sustainable toxoplasmosis control.
文摘With the purpose to determine the frequency and type of cardiac lesions in naturally exposed dogs to Trypanosoma cruzi, ninety one stray dogs, capture by the Canine and Feline Control Center （dog pound） from the municipality of Merida, were studied. Before euthanasia, blood samples were taken to detect 72 cruzi antigens by indirect immunofluorescence antibody test and Western Blot and to detect the genome of parasite by Polymerase Chain Reaction. Immediately after euthanasia, hearts were macroscopically evaluated and a sample of the middle right atrial wall of each dog was taken for histopathological analyses. DNA was also obtained from paraffin blocks of seropositives animals with microscopic lesions to detect 72 cruzi genome. Of ninety one dogs, thirteen were seropositive. All seropositive dogs showed an association （P 〈 0.05） with lymphocytoplasmatic myocarditis. The presence of the 72 cruzi genome was also detected by PCR in cardiac septum tissue of seropositive dogs and in all the cases with microscopic lesions indicating the high pathogenicity of the local circulating strain. No association with macroscopic lesions was observed in seropositive dogs. Also, the presence of Dirofilaria immitis （D. immitis） was found in 6% of dogs evaluated. This study demonstrates a high tropism to cardiac tissue and virulence of the strains of 72 cruzi circulating in the studied dog population.
