Objective:To determine the expression of the synaptonemal complex protein-3 (SYCP3) gene in men with nonobstructive azoospermia. Design:Cross-sectional case study. Setting:Avesina Infertility Clinic,Tehran,Iran. Patie...Objective:To determine the expression of the synaptonemal complex protein-3 (SYCP3) gene in men with nonobstructive azoospermia. Design:Cross-sectional case study. Setting:Avesina Infertility Clinic,Tehran,Iran. Patient(s):One hundred and ten consecutive infertile men presenting nonobstructive azoospermia. Intervention(s):Testicular biopsies for histopathological assessment and analyses of SYCP3 expression level by semiquantitative nested reverse transcription-polymerase chain reaction (RT-PCR). The SYCP3 levels were normalized to expression of the housekeeping phosphoglucomutase 1 gene. Main Outcome Measure(s):Expression of SYCP3 messenger ribonucleic acid (mRNA). Correlation of the histopathological findings with SYCP3 expression levels. Results(s):Testicular SYCP3 mRNA expression was observed in 67/110 (60.9%) patients. The expression level correlated with the degree of spermatogenic failure. Although it was expressed in patients with spermatogenesis and maturation arrest,a lack of expression was seen in all of those men with spermatogonial arrest,Sertoli cell-only syndrome,and testicular atrophy. Conclusion(s):These data indicate that SYCP3 is expressed in human testis and is restricted to germ cells. Our findings,in association with those obtained in experimental animals,shows that lack of SYCP3 expression in human testis may have a negative effect on spermatogenesis and male fertility.展开更多
Objective To report on the treatment with recombinant FSH of an azoos permic no rmogonadotropic patient with a Y-chromosome microdeletion. Design Case report. Setting An assisted reproduction center. Patient(s) A 32-y...Objective To report on the treatment with recombinant FSH of an azoos permic no rmogonadotropic patient with a Y-chromosome microdeletion. Design Case report. Setting An assisted reproduction center. Patient(s) A 32-year-old patient with azoospermia. Intervention(s) Histological testicular biopsy, Ychromosome screen ing, FSH treatment, and intracytoplasmic sperm injection. Main outcome measure(s ) Fertilization, embryo development, pregnancy, and delivery. Result(s) After 6 months of treatment with gonadotropins, a small number of spermatozoa were found in the patients ejaculate and used for IVF, resulting in a successful twin pr egnancy and the delivery of two healthy girls. Conclusion(s) In this study, trea tment with gonadotropins promoted the spermatogenesis process and led to the pro duction of spermatozoa in a normogonadotropicazoospermic patient.展开更多
文摘Objective:To determine the expression of the synaptonemal complex protein-3 (SYCP3) gene in men with nonobstructive azoospermia. Design:Cross-sectional case study. Setting:Avesina Infertility Clinic,Tehran,Iran. Patient(s):One hundred and ten consecutive infertile men presenting nonobstructive azoospermia. Intervention(s):Testicular biopsies for histopathological assessment and analyses of SYCP3 expression level by semiquantitative nested reverse transcription-polymerase chain reaction (RT-PCR). The SYCP3 levels were normalized to expression of the housekeeping phosphoglucomutase 1 gene. Main Outcome Measure(s):Expression of SYCP3 messenger ribonucleic acid (mRNA). Correlation of the histopathological findings with SYCP3 expression levels. Results(s):Testicular SYCP3 mRNA expression was observed in 67/110 (60.9%) patients. The expression level correlated with the degree of spermatogenic failure. Although it was expressed in patients with spermatogenesis and maturation arrest,a lack of expression was seen in all of those men with spermatogonial arrest,Sertoli cell-only syndrome,and testicular atrophy. Conclusion(s):These data indicate that SYCP3 is expressed in human testis and is restricted to germ cells. Our findings,in association with those obtained in experimental animals,shows that lack of SYCP3 expression in human testis may have a negative effect on spermatogenesis and male fertility.
文摘Objective To report on the treatment with recombinant FSH of an azoos permic no rmogonadotropic patient with a Y-chromosome microdeletion. Design Case report. Setting An assisted reproduction center. Patient(s) A 32-year-old patient with azoospermia. Intervention(s) Histological testicular biopsy, Ychromosome screen ing, FSH treatment, and intracytoplasmic sperm injection. Main outcome measure(s ) Fertilization, embryo development, pregnancy, and delivery. Result(s) After 6 months of treatment with gonadotropins, a small number of spermatozoa were found in the patients ejaculate and used for IVF, resulting in a successful twin pr egnancy and the delivery of two healthy girls. Conclusion(s) In this study, trea tment with gonadotropins promoted the spermatogenesis process and led to the pro duction of spermatozoa in a normogonadotropicazoospermic patient.
