采用2×2实验设计,研究不同家系种质(G♂×S♀、S♂×S♀)和日粮蛋白含量(37%、40%)对黄颡鱼幼鱼生长性能及肝脏IGF-I m RNA表达水平的影响及其交互作用。各试验组设3个平行,养殖60 d。分析结果显示:日粮蛋白含量、家系两...采用2×2实验设计,研究不同家系种质(G♂×S♀、S♂×S♀)和日粮蛋白含量(37%、40%)对黄颡鱼幼鱼生长性能及肝脏IGF-I m RNA表达水平的影响及其交互作用。各试验组设3个平行,养殖60 d。分析结果显示:日粮蛋白含量、家系两因素对末重、增重率、特定生长率及IGF-I m RNA的相对表达量等指标均影响显著(P<0.05),两因素在对上述指标的影响上均不存在交互作用。P40/(G♂×S♀)获得最大末重、增重率和IGF-I基因表达量。结果表明黄颡鱼幼鱼可通过种质筛选和日粮蛋白营养调节达到更好的养殖效果,G♂×S♀家系在高蛋白日粮投喂下,获得最佳生长性能。展开更多
The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with ...The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein(47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0℃, salinity was controlled between 28.5 and 32.0, acidity was controlled between p H7.8 and p H8.5, and ammonia nitrogen was maintained below 0.03 mg L-1 and dissolved oxygen was maintained about 7 mg L-1. Results showed that the growth of fish was significantly affected by dietary protein content(P < 0.05). Specific growth rate(SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0%(P < 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets(P > 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%.展开更多
文摘采用2×2实验设计,研究不同家系种质(G♂×S♀、S♂×S♀)和日粮蛋白含量(37%、40%)对黄颡鱼幼鱼生长性能及肝脏IGF-I m RNA表达水平的影响及其交互作用。各试验组设3个平行,养殖60 d。分析结果显示:日粮蛋白含量、家系两因素对末重、增重率、特定生长率及IGF-I m RNA的相对表达量等指标均影响显著(P<0.05),两因素在对上述指标的影响上均不存在交互作用。P40/(G♂×S♀)获得最大末重、增重率和IGF-I基因表达量。结果表明黄颡鱼幼鱼可通过种质筛选和日粮蛋白营养调节达到更好的养殖效果,G♂×S♀家系在高蛋白日粮投喂下,获得最佳生长性能。
基金supported by the earmarked fund for Modern Agro-Industry Technology Research System with Grant No. CARS 50-G-08
文摘The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein(47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0℃, salinity was controlled between 28.5 and 32.0, acidity was controlled between p H7.8 and p H8.5, and ammonia nitrogen was maintained below 0.03 mg L-1 and dissolved oxygen was maintained about 7 mg L-1. Results showed that the growth of fish was significantly affected by dietary protein content(P < 0.05). Specific growth rate(SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0%(P < 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets(P > 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%.
