AIM: We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4). METHODS: Ovari...AIM: We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4). METHODS: Ovariectomized adult female gerbils were subcutaneously treated with E2, and E2 + P4. Uteri and stomachs were removed, the latter were cut along the greater curvature, and antrum and corpus were excised. Proteins were immunoblotted using antibodies that recognize ER-alpha, ER-beta, and PR-A and PR-B receptor isoforms. Tissues from rats treated in the same way were used as controls. RESULTS: Specific bands were detected for ER-alpha (68 KDa), and PR isoforms (85 and 120 KDa for PR-A and PR-B isoforms, respectively) in uteri, gastric antrum and corpus. We could not detect ER-beta isoform. PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils. ER-alpha isoform content was significantly down-regulated by E2 in the corpus, but not affected by hormones in uterus and gastric antrum. CONCLUSION: The presence of ER-alpha and PR isoforms in gerbils stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.展开更多
近日,来自多伦多玛格丽特公主癌症中心（Princess Margaret Cancer Centre,PMCC）的研究人员已经发现了促进乳腺干细胞响应性激素黄体酮增殖的表观遗传学蛋白。这项最新发表在《Journal of Cell Biology》的研究表明使用药物抑制这些蛋...近日,来自多伦多玛格丽特公主癌症中心（Princess Margaret Cancer Centre,PMCC）的研究人员已经发现了促进乳腺干细胞响应性激素黄体酮增殖的表观遗传学蛋白。这项最新发表在《Journal of Cell Biology》的研究表明使用药物抑制这些蛋白质可以防止乳腺癌高风险的女性的疾病进展。乳腺包含两种细胞：腔细胞和基底细胞,这些细胞都来自特殊的干细胞或者前体细胞。展开更多
文摘AIM: We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4). METHODS: Ovariectomized adult female gerbils were subcutaneously treated with E2, and E2 + P4. Uteri and stomachs were removed, the latter were cut along the greater curvature, and antrum and corpus were excised. Proteins were immunoblotted using antibodies that recognize ER-alpha, ER-beta, and PR-A and PR-B receptor isoforms. Tissues from rats treated in the same way were used as controls. RESULTS: Specific bands were detected for ER-alpha (68 KDa), and PR isoforms (85 and 120 KDa for PR-A and PR-B isoforms, respectively) in uteri, gastric antrum and corpus. We could not detect ER-beta isoform. PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils. ER-alpha isoform content was significantly down-regulated by E2 in the corpus, but not affected by hormones in uterus and gastric antrum. CONCLUSION: The presence of ER-alpha and PR isoforms in gerbils stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.
文摘近日,来自多伦多玛格丽特公主癌症中心（Princess Margaret Cancer Centre,PMCC）的研究人员已经发现了促进乳腺干细胞响应性激素黄体酮增殖的表观遗传学蛋白。这项最新发表在《Journal of Cell Biology》的研究表明使用药物抑制这些蛋白质可以防止乳腺癌高风险的女性的疾病进展。乳腺包含两种细胞：腔细胞和基底细胞,这些细胞都来自特殊的干细胞或者前体细胞。
