本文主要研究了阳离子gemini型表面活性剂C12CsC12Br2 (s = 2、6和10)在稀溶液状态下对α-糜蛋白酶活性的影响。在稀溶液体系中我们选择了几种具有典型间隔链长度的gemini表面活性剂12-s-12 (s = 2、6和10)探究不同头基距离的表面活性...本文主要研究了阳离子gemini型表面活性剂C12CsC12Br2 (s = 2、6和10)在稀溶液状态下对α-糜蛋白酶活性的影响。在稀溶液体系中我们选择了几种具有典型间隔链长度的gemini表面活性剂12-s-12 (s = 2、6和10)探究不同头基距离的表面活性剂与α-CT之间不同程度的相互作用,同时将传统单链阳离子表面活性剂DTAB作为对比研究。结果表明,12-s-12/α-CT体系在所研究的浓度范围内均出现“钟形”的超活性区域,催化效果比DTAB/α-CT要好。α-CT相对活性的大小与gemini型表面活性剂的间隔链长度也有关系,即spacer越长,头基的电荷密度相对越小,超活性出现的越早而且最大相对活性值也越高。In this work we studied the catalytic activity of α-chymotrypsin (α-CT) in gemini surfactants C12CsC12Br2 (s = 2, 6, 10) solution. We selected several cationic gemini surfactants (12-s-12, s = 2, 6, 10) to investigate the effects of different spacer lengths on the activity of α-chymotrypsin, meanwhile the conventional single-chain cationic surfactant DTAB was also used as a comparative study. In all cases, the superactivity of 12-s-12/α-CT system appears in a bell-shape in a concentration region below their respective critical micelle concentration and, the catalytic effect is better than that of DTAB/α-CT system. The relative activity of α-CT was related to the spacer chain length of the gemini surfactant, that is, the gemini surfactant with longer spacer might have a much stronger influence on the enzyme activity due to larger size and its stronger hydrophobicity of its head group.展开更多
目的:探讨10%西甲硅油联合糜蛋白酶配制成胃镜消泡剂的祛泡效果。方法:将300例胃镜检查患者随机分组:西甲硅油+糜蛋白酶组和西甲硅油组。检查前20 min,西甲硅油+糜蛋白酶组予(0.9%生理盐水27 m L+西甲硅油3 m L+糜蛋白酶200 U)稀释溶液3...目的:探讨10%西甲硅油联合糜蛋白酶配制成胃镜消泡剂的祛泡效果。方法:将300例胃镜检查患者随机分组:西甲硅油+糜蛋白酶组和西甲硅油组。检查前20 min,西甲硅油+糜蛋白酶组予(0.9%生理盐水27 m L+西甲硅油3 m L+糜蛋白酶200 U)稀释溶液30 m L口服;西甲硅油组予(0.9%生理盐水27 m L+西甲硅油3 m L)稀释溶液30 m L口服。比较两组患者胃镜检查视野清晰度、检查时间、医生满意度,对两组胃镜检查消泡剂的祛泡效果进行研究分析。结果:西甲硅油+糜蛋白酶组胃镜检查视野清晰度、医生满意度高于西甲硅油组(P<0.05﹚,胃镜检查时间短于西甲硅油组(P<0.05﹚。结论:西甲硅油联合糜蛋白酶作为胃镜检查消泡剂的祛泡效果比单独使用西甲硅油效果更好,胃镜检查时间更短,医生满意度更高,是值得临床推广使用的祛泡方法。展开更多
文摘本文主要研究了阳离子gemini型表面活性剂C12CsC12Br2 (s = 2、6和10)在稀溶液状态下对α-糜蛋白酶活性的影响。在稀溶液体系中我们选择了几种具有典型间隔链长度的gemini表面活性剂12-s-12 (s = 2、6和10)探究不同头基距离的表面活性剂与α-CT之间不同程度的相互作用,同时将传统单链阳离子表面活性剂DTAB作为对比研究。结果表明,12-s-12/α-CT体系在所研究的浓度范围内均出现“钟形”的超活性区域,催化效果比DTAB/α-CT要好。α-CT相对活性的大小与gemini型表面活性剂的间隔链长度也有关系,即spacer越长,头基的电荷密度相对越小,超活性出现的越早而且最大相对活性值也越高。In this work we studied the catalytic activity of α-chymotrypsin (α-CT) in gemini surfactants C12CsC12Br2 (s = 2, 6, 10) solution. We selected several cationic gemini surfactants (12-s-12, s = 2, 6, 10) to investigate the effects of different spacer lengths on the activity of α-chymotrypsin, meanwhile the conventional single-chain cationic surfactant DTAB was also used as a comparative study. In all cases, the superactivity of 12-s-12/α-CT system appears in a bell-shape in a concentration region below their respective critical micelle concentration and, the catalytic effect is better than that of DTAB/α-CT system. The relative activity of α-CT was related to the spacer chain length of the gemini surfactant, that is, the gemini surfactant with longer spacer might have a much stronger influence on the enzyme activity due to larger size and its stronger hydrophobicity of its head group.
文摘目的:探讨10%西甲硅油联合糜蛋白酶配制成胃镜消泡剂的祛泡效果。方法:将300例胃镜检查患者随机分组:西甲硅油+糜蛋白酶组和西甲硅油组。检查前20 min,西甲硅油+糜蛋白酶组予(0.9%生理盐水27 m L+西甲硅油3 m L+糜蛋白酶200 U)稀释溶液30 m L口服;西甲硅油组予(0.9%生理盐水27 m L+西甲硅油3 m L)稀释溶液30 m L口服。比较两组患者胃镜检查视野清晰度、检查时间、医生满意度,对两组胃镜检查消泡剂的祛泡效果进行研究分析。结果:西甲硅油+糜蛋白酶组胃镜检查视野清晰度、医生满意度高于西甲硅油组(P<0.05﹚,胃镜检查时间短于西甲硅油组(P<0.05﹚。结论:西甲硅油联合糜蛋白酶作为胃镜检查消泡剂的祛泡效果比单独使用西甲硅油效果更好,胃镜检查时间更短,医生满意度更高,是值得临床推广使用的祛泡方法。
