Blood ketone body level and ketone body ratio (acetoacetate/β hydroxybutyrate) are commonly used as clinical diagnostic indices.But there is no systematic study on the variability of these indices in the blood circul...Blood ketone body level and ketone body ratio (acetoacetate/β hydroxybutyrate) are commonly used as clinical diagnostic indices.But there is no systematic study on the variability of these indices in the blood circulation.We investigated the concentration of ketone bodies within different segments of the circulation in fed ad libitum rats, starved rats and alloxan induced diabetic rats.Blood samples were drawn from femoral artery, suprarenal inferior vena cava and hepatic vein of each rat, respectively.The concentrations of acetoacetate and β hydroxybutyrate were measured by enzymatic method.To avoid mixing of the blood streams, appropriate strangulation of the vessels was taken when sampling.The total ketone body level was always the highest in the hepatic vein, secondary in the femoral artery and the lowest in the suprarenal vena cava in rats in different states.But surprisingly, the concentration of acetoacetate was the lowest in the hepatic vein and the highest in the femoral artery.The ketone body levels elevated in both starvation and diabetes, and the ketone body ratios were changed in the circulation.While blood flows through extra hepatic tissues, the total concentration of ketone body is undergoing degression, but the concentration of acetoacetate could be elevated due to the conversion of ketone body in extra hepatic tissues.Therefore, ketone body ratio is changeable in different segments of the blood circulation, including that between hepatic vein and artery.The correlation of ketone body ratio between arterial and hepatic venous blood could be effected by different pathophysiological states, such as starvation and diabetes.The “Redox theory” deserves a further discussion.展开更多
对52例肝癌患者手术后进行动脉血酮体比率(K B R)及常规肝功能测定。其中A组(K B R 0.7以上)术后并发症3%,B组( KB R低达0.4～0.7)并发症33.3%,C组(K B R逐渐下降到0.4),并发多种严重并发症,最终死于M O F。常规肝功能各项指标在上述三...对52例肝癌患者手术后进行动脉血酮体比率(K B R)及常规肝功能测定。其中A组(K B R 0.7以上)术后并发症3%,B组( KB R低达0.4～0.7)并发症33.3%,C组(K B R逐渐下降到0.4),并发多种严重并发症,最终死于M O F。常规肝功能各项指标在上述三组中的变化无明显差异,提示常规肝功能并不能定量反映肝功能损害的程度,而K B R可反映肝脏线粒体氧化磷酸化状态,可直接反映术后肝衰的变化。展开更多
文摘Blood ketone body level and ketone body ratio (acetoacetate/β hydroxybutyrate) are commonly used as clinical diagnostic indices.But there is no systematic study on the variability of these indices in the blood circulation.We investigated the concentration of ketone bodies within different segments of the circulation in fed ad libitum rats, starved rats and alloxan induced diabetic rats.Blood samples were drawn from femoral artery, suprarenal inferior vena cava and hepatic vein of each rat, respectively.The concentrations of acetoacetate and β hydroxybutyrate were measured by enzymatic method.To avoid mixing of the blood streams, appropriate strangulation of the vessels was taken when sampling.The total ketone body level was always the highest in the hepatic vein, secondary in the femoral artery and the lowest in the suprarenal vena cava in rats in different states.But surprisingly, the concentration of acetoacetate was the lowest in the hepatic vein and the highest in the femoral artery.The ketone body levels elevated in both starvation and diabetes, and the ketone body ratios were changed in the circulation.While blood flows through extra hepatic tissues, the total concentration of ketone body is undergoing degression, but the concentration of acetoacetate could be elevated due to the conversion of ketone body in extra hepatic tissues.Therefore, ketone body ratio is changeable in different segments of the blood circulation, including that between hepatic vein and artery.The correlation of ketone body ratio between arterial and hepatic venous blood could be effected by different pathophysiological states, such as starvation and diabetes.The “Redox theory” deserves a further discussion.
文摘对52例肝癌患者手术后进行动脉血酮体比率(K B R)及常规肝功能测定。其中A组(K B R 0.7以上)术后并发症3%,B组( KB R低达0.4～0.7)并发症33.3%,C组(K B R逐渐下降到0.4),并发多种严重并发症,最终死于M O F。常规肝功能各项指标在上述三组中的变化无明显差异,提示常规肝功能并不能定量反映肝功能损害的程度,而K B R可反映肝脏线粒体氧化磷酸化状态,可直接反映术后肝衰的变化。
