[Objective] The study aimed to evaluate the genetic variability and interspecific relationships among four species of groupers from South China Sea, including E. fario, E. merra, E. malabaricus and E. coioides. [Metho...[Objective] The study aimed to evaluate the genetic variability and interspecific relationships among four species of groupers from South China Sea, including E. fario, E. merra, E. malabaricus and E. coioides. [Method] Twenty one mircosatellite loci of groupers were selected from GenBank and eight high polymorphic loci were used to further genetic analysis. [Result] The mean number of alleles per locus （A）, effective number of alleles （Ne）, mean polymorphism information content （PIC）, observed heterozygosity （Ho） and expected heterozygosity （He） were 4.38±1.60, 3.69±0.86, 0.69±0.08, 0.67±0.08, 0.72±0.06 in E. malabaricus; 3.88±1.13, 3.55±1.04, 0.66±0.10, 0.68±0.21, 0.70±0.08 in E.coioides; 6.00±1.07, 4.68±0.65, 0.78±0.03, 0.73±0.25, 0.79±0.03 in E. fario; 5.50±1.07, 4.58±0.80, 0.76±0.05, 0.75±0.18, 0.78±0.04 in E. merra, respectively. [Conclusion] We compared the values above, the order of the genetic variability among these grouper species was E. fario E. merra E. malabaricus E. coioides. We found that the level of genetic variability of these groupers species was relatively higher than that of other marine fish, so their genetic status was good. In addition, the analyisis of genetic relationship showed that E. malabaricus and E. coioides was the closest and it was the farthest between E. merra and E. coioides.展开更多
A stochastic finite element computational methodology for probabilistic durability assessment of deteriorating reinforced concrete（RC） bridges by considering the time-and space-dependent variabilities is presented.F...A stochastic finite element computational methodology for probabilistic durability assessment of deteriorating reinforced concrete（RC） bridges by considering the time-and space-dependent variabilities is presented.First,finite element analysis with a smeared cracking approach is implemented.The time-dependent bond-slip relationship between steel and concrete,and the stress-strain relationship of corroded steel bars are considered.Secondly,a stochastic finite element-based computational framework for reliability assessment of deteriorating RC bridges is proposed.The spatial and temporal variability of several parameters affecting the reliability of RC bridges is considered.Based on the data reported by several researchers and from field investigations,the Monte Carlo simulation is used to account for the uncertainties in various parameters,including local and general corrosion in rebars,concrete cover depth,surface chloride concentration,chloride diffusion coefficient,and corrosion rate.Finally,the proposed probabilistic durability assessment approach and framework are applied to evaluate the time-dependent reliability of a girder of a RC bridge located on the Tianjin Binhai New Area in China.展开更多
The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (including 5.8S rRNA) of 15 Rhododendron, species, representing most sections of the genus, one Ledum species and Cassiope fastigiata were sequenc...The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (including 5.8S rRNA) of 15 Rhododendron, species, representing most sections of the genus, one Ledum species and Cassiope fastigiata were sequenced. Together with the ITS sequences of 13 selected Rhododendron species and Bejaria racemosa downloaded from GenBank, we explored the infrageneric and sectional relationships of this important North Temperate genus by employing maximum-parsimony analysis using PAUP software. C. fastigiata and B. racemosa were designated as outgroups. The ITS-based tree inferred that: (1) Rhododendron was a well-supported monophyletic group, while subg. Therorhodion was basal to the rest of the genus; (2) Ledum was a member of Rhododendron, and its close relationship with the lepidote rhododendron was confirmed; (3) the lepidote rhododendron plus Ledum formed a strongly-supported monophyletic clade which was sister to the rest of the elepidote rhododendron; (4) the elepidote rhododendron formed a weakly-supported clade within which the monophyly of subg. Hynwrianthes and subg. Tsutsusi were strongly supported, while subg. Pentanthera and subg. Azaleastrum were polyphyletic; and (5) the monophyly of sect. Choniastnini, (subg. Azaleastrum) was strongly-supported, while subg. Tsutsusi could be sister to a weakly-supported clade composed of two sampled species of sect. Azaleastrum (subg. Azaleastrum) together with R. sentibarbatum, of subg. Mumeazalea.展开更多
Genetic relationships of eight species of genus Betula were evaluated using ISSR marks. A total of 236 loci were generated from 17 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93 to 19.92. The hig...Genetic relationships of eight species of genus Betula were evaluated using ISSR marks. A total of 236 loci were generated from 17 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93 to 19.92. The highest and the lowest level of genetic differentiation were detected in B. ovalifolia and B. maximowicziana Regel respectively. In these eight species, genetic diversity of birch (HT) was 24.38 %, and the genetic variation (GST ) interspecies was accounting for 79.36% of total genetic variation. According to the cluster results of genetic distance, the eight species were classified into three groups as B. davurica, B. ovalifolia, B. platyphylla and B. pendula for one group; B. schmidtii, B. costata and B. ermanii Cham. var. communis for one group, and B. maximowicziana Regel for another group. The result of cluster is consistent with traditional morphological classification.展开更多
To extend the traditional generalized grey incidence model, a novel grey incidence model based on inter- val grey numbers is constructed. Considering the numerical information of indexes cannot be accurately obtained ...To extend the traditional generalized grey incidence model, a novel grey incidence model based on inter- val grey numbers is constructed. Considering the numerical information of indexes cannot be accurately obtained and can be defined as interval grey numbers, the interval grey numbers are defined as standard interval grey num- bers which are split in white part and grey part. The absolute degree of incidence and relative degree of incidence based on the interval grey numbers are constructed and their arithmetic are given. Finally, an example about commercial aircraft index selection illuminates the effectiveness of the model. The results show that the model can sort indexes better and can extend the grey incidence models significantly.展开更多
To study the congestion of interrupted flow on urban roads, a comprehensive evaluation method is proposed. First, based on the results of correlation analysis between different parameters of interrupted flow, the traf...To study the congestion of interrupted flow on urban roads, a comprehensive evaluation method is proposed. First, based on the results of correlation analysis between different parameters of interrupted flow, the traffic parameters of interrupted traffic flow are divided into two categories： the basic parameters and the operation parameters. Polynomial regression is used to formulize the nonlinear relationships between the basic parameters and the operation parameters. Then, the congestion model incorporating both operational and volume characteristics of traffic flow is proposed. The inputs of the model are the basic parameters, while the output is a dimensionless index value between 0 and 1. Finally, the proposed methods are compared with existing evaluation measures of congestion. Results show that the proposed indices can capture the variation of both the basic parameters and the operation parameters, which is more balanced compared with the existing evaluation measures.展开更多
Pinus squamata X. W. Li, a recently-described species of the Pinaceae, is an extremely endangered pine with only 32 individuals in the wild. This species was thought to be intermediate between P. bungeana Zuec. ex End...Pinus squamata X. W. Li, a recently-described species of the Pinaceae, is an extremely endangered pine with only 32 individuals in the wild. This species was thought to be intermediate between P. bungeana Zuec. ex Endl. of subgen. Strobus and P. yunnanensis Franch. of subgen. Pinus by the original author. We made an effort to address the question of the phylogenetic relationship of this peculiar and important species in the context of the major groups of the genus Pinus by using sequences of the rbcL, matK,genes, rpl20-rps18 spacer, trnV intron of the chloroplast genome and the nuclear ribosomal ITS region. The results of the separated analysis and the combined analysis of the four cpDNA sequences and ITS sequence indicated that P. squamata was a stable member of subsect. Gerardianae and P. gerardiana Wall. was the closest species of P. squamata phylogenetically. As a result, the causes of the distribution pattern of subsect. Gerardianae were also discussed.展开更多
Spatial structure of overlying strata existed and evolved dynamically with the exploitation scope （boundary conditions） changes in coal mines and to induce rockburst. Based on the“key strata”theory, the integrated...Spatial structure of overlying strata existed and evolved dynamically with the exploitation scope （boundary conditions） changes in coal mines and to induce rockburst. Based on the“key strata”theory, the integrated spatial structure of overlying strata was put forward, which was composed of “O-X” structure in the plane section and “F” structure in the vertical section. The formation and ongoing instability of the“O-X”and“F”structures were called as dynamic evolution cycle of the overlying strata. Three basic categories of “O-X”, “F” and “T” structures were defined, and the strata behaviors of each spatial structure were analyzed. According to energy theory, mechanism of rockburst induced by spatial structure instability was discussed. The research expanded the scope of traditional ground pressure theory and provided a guide for the prevention of rockburst and mining tremors induced by structure instability of overlying展开更多
A positioning volume ellipsoid method is proposed to represent tumor volume in the workspace of a high-intensity-focused ultrasound (HIFU) surgery platform during the platform's positioning motion. To this simplif...A positioning volume ellipsoid method is proposed to represent tumor volume in the workspace of a high-intensity-focused ultrasound (HIFU) surgery platform during the platform's positioning motion. To this simplified tumor model, a nearest neighbor search method is used to determine intermediate configuration and goal configuration, which the treatment head and ultrasound focus must pass in their localization to target volume. Based on the decided configurations, the continuity condition of combined Bezier curve in Euclidean space and De Casteljau algorithm on Lie group SO(3) are integrated to construct a combined Bezier positioning path of C2 continuity at junctional configuration, and an illustration of different positioning path planning is analyzed based on a liver tumor case.展开更多
A constrained partial permutation strategy is proposed for matching spatial relation graph (SRG), which is used in our sketch input and recognition system Smart Sketchpad for representing the spatial relationship amon...A constrained partial permutation strategy is proposed for matching spatial relation graph (SRG), which is used in our sketch input and recognition system Smart Sketchpad for representing the spatial relationship among the components of a graphic object. Using two kinds of matching constraints dynamically generated in the matching process, the proposed approach can prune most improper mappings between SRGs during the matching process. According to our theoretical analysis in this paper, the time complexity of our approach is O(n 2) in the best case, and O(n!) in the worst case, which occurs infrequently. The spatial complexity is always O(n) for all cases. Implemented in Smart Sketchpad, our proposed strategy is of good performance.展开更多
基金Supported by the National High Technology Research and Development Program of China(863Program,No.2007AA091404)the National Key Technology Research and Development Program(2007BAD29B03)A Project Funded by the Academic Program Development of Jiangsu Higher Education Institutions(PAPD)~~
文摘[Objective] The study aimed to evaluate the genetic variability and interspecific relationships among four species of groupers from South China Sea, including E. fario, E. merra, E. malabaricus and E. coioides. [Method] Twenty one mircosatellite loci of groupers were selected from GenBank and eight high polymorphic loci were used to further genetic analysis. [Result] The mean number of alleles per locus （A）, effective number of alleles （Ne）, mean polymorphism information content （PIC）, observed heterozygosity （Ho） and expected heterozygosity （He） were 4.38±1.60, 3.69±0.86, 0.69±0.08, 0.67±0.08, 0.72±0.06 in E. malabaricus; 3.88±1.13, 3.55±1.04, 0.66±0.10, 0.68±0.21, 0.70±0.08 in E.coioides; 6.00±1.07, 4.68±0.65, 0.78±0.03, 0.73±0.25, 0.79±0.03 in E. fario; 5.50±1.07, 4.58±0.80, 0.76±0.05, 0.75±0.18, 0.78±0.04 in E. merra, respectively. [Conclusion] We compared the values above, the order of the genetic variability among these grouper species was E. fario E. merra E. malabaricus E. coioides. We found that the level of genetic variability of these groupers species was relatively higher than that of other marine fish, so their genetic status was good. In addition, the analyisis of genetic relationship showed that E. malabaricus and E. coioides was the closest and it was the farthest between E. merra and E. coioides.
基金The National Natural Science Foundation of China (No.50708065)the National High Technology Research and Development Program of China (863 Program) (No. 2007AA11Z113)Specialized Research Fund for the Doctoral Program of Higher Education (No. 20070056125)
文摘A stochastic finite element computational methodology for probabilistic durability assessment of deteriorating reinforced concrete（RC） bridges by considering the time-and space-dependent variabilities is presented.First,finite element analysis with a smeared cracking approach is implemented.The time-dependent bond-slip relationship between steel and concrete,and the stress-strain relationship of corroded steel bars are considered.Secondly,a stochastic finite element-based computational framework for reliability assessment of deteriorating RC bridges is proposed.The spatial and temporal variability of several parameters affecting the reliability of RC bridges is considered.Based on the data reported by several researchers and from field investigations,the Monte Carlo simulation is used to account for the uncertainties in various parameters,including local and general corrosion in rebars,concrete cover depth,surface chloride concentration,chloride diffusion coefficient,and corrosion rate.Finally,the proposed probabilistic durability assessment approach and framework are applied to evaluate the time-dependent reliability of a girder of a RC bridge located on the Tianjin Binhai New Area in China.
文摘The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (including 5.8S rRNA) of 15 Rhododendron, species, representing most sections of the genus, one Ledum species and Cassiope fastigiata were sequenced. Together with the ITS sequences of 13 selected Rhododendron species and Bejaria racemosa downloaded from GenBank, we explored the infrageneric and sectional relationships of this important North Temperate genus by employing maximum-parsimony analysis using PAUP software. C. fastigiata and B. racemosa were designated as outgroups. The ITS-based tree inferred that: (1) Rhododendron was a well-supported monophyletic group, while subg. Therorhodion was basal to the rest of the genus; (2) Ledum was a member of Rhododendron, and its close relationship with the lepidote rhododendron was confirmed; (3) the lepidote rhododendron plus Ledum formed a strongly-supported monophyletic clade which was sister to the rest of the elepidote rhododendron; (4) the elepidote rhododendron formed a weakly-supported clade within which the monophyly of subg. Hynwrianthes and subg. Tsutsusi were strongly supported, while subg. Pentanthera and subg. Azaleastrum were polyphyletic; and (5) the monophyly of sect. Choniastnini, (subg. Azaleastrum) was strongly-supported, while subg. Tsutsusi could be sister to a weakly-supported clade composed of two sampled species of sect. Azaleastrum (subg. Azaleastrum) together with R. sentibarbatum, of subg. Mumeazalea.
基金This paper was supported by Northeast Forestry University.
文摘Genetic relationships of eight species of genus Betula were evaluated using ISSR marks. A total of 236 loci were generated from 17 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93 to 19.92. The highest and the lowest level of genetic differentiation were detected in B. ovalifolia and B. maximowicziana Regel respectively. In these eight species, genetic diversity of birch (HT) was 24.38 %, and the genetic variation (GST ) interspecies was accounting for 79.36% of total genetic variation. According to the cluster results of genetic distance, the eight species were classified into three groups as B. davurica, B. ovalifolia, B. platyphylla and B. pendula for one group; B. schmidtii, B. costata and B. ermanii Cham. var. communis for one group, and B. maximowicziana Regel for another group. The result of cluster is consistent with traditional morphological classification.
基金Supported by the National Natural Science Foundation of China(70901041,71171113)the Joint Research Project of National Natural Science Foundation of China and Royal Society of UK(71111130211)+3 种基金the Major Program of National Funds of Social Science of Chinathe Doctoral Fund of Ministry of Education of China(20093218120032,200802870020)the Qinglan Project for Excellent Youth Teacher in Jiangsu Province(China)the Research Funding of Nanjing University of Aeronautics and Astronautics(NR2011002,NJ2011009)~~
文摘To extend the traditional generalized grey incidence model, a novel grey incidence model based on inter- val grey numbers is constructed. Considering the numerical information of indexes cannot be accurately obtained and can be defined as interval grey numbers, the interval grey numbers are defined as standard interval grey num- bers which are split in white part and grey part. The absolute degree of incidence and relative degree of incidence based on the interval grey numbers are constructed and their arithmetic are given. Finally, an example about commercial aircraft index selection illuminates the effectiveness of the model. The results show that the model can sort indexes better and can extend the grey incidence models significantly.
基金The National High Technology Research and Development Program of China(863 Program)(No.2011AA110302-01)
文摘To study the congestion of interrupted flow on urban roads, a comprehensive evaluation method is proposed. First, based on the results of correlation analysis between different parameters of interrupted flow, the traffic parameters of interrupted traffic flow are divided into two categories： the basic parameters and the operation parameters. Polynomial regression is used to formulize the nonlinear relationships between the basic parameters and the operation parameters. Then, the congestion model incorporating both operational and volume characteristics of traffic flow is proposed. The inputs of the model are the basic parameters, while the output is a dimensionless index value between 0 and 1. Finally, the proposed methods are compared with existing evaluation measures of congestion. Results show that the proposed indices can capture the variation of both the basic parameters and the operation parameters, which is more balanced compared with the existing evaluation measures.
文摘Pinus squamata X. W. Li, a recently-described species of the Pinaceae, is an extremely endangered pine with only 32 individuals in the wild. This species was thought to be intermediate between P. bungeana Zuec. ex Endl. of subgen. Strobus and P. yunnanensis Franch. of subgen. Pinus by the original author. We made an effort to address the question of the phylogenetic relationship of this peculiar and important species in the context of the major groups of the genus Pinus by using sequences of the rbcL, matK,genes, rpl20-rps18 spacer, trnV intron of the chloroplast genome and the nuclear ribosomal ITS region. The results of the separated analysis and the combined analysis of the four cpDNA sequences and ITS sequence indicated that P. squamata was a stable member of subsect. Gerardianae and P. gerardiana Wall. was the closest species of P. squamata phylogenetically. As a result, the causes of the distribution pattern of subsect. Gerardianae were also discussed.
基金Project (2013QNB30) supported by the Fundamental Research Funds for Central Universities,ChinaProject (2010CB226805) supported by the National Basic Research Program of China+3 种基金Project (51174285) supported by the National Natural Science Foundation of ChinaProject (2012BAK09B01) supported by the Twelfth Five-Year National Key Technology R&D Program,ChinaProject (SZBF2011-6-B35) supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions,ChinaProject (SKLCRSM10X05) supported by the Independent Foundation of State Key Laboratory of Coal Resources and Safe Mining,China
文摘Spatial structure of overlying strata existed and evolved dynamically with the exploitation scope （boundary conditions） changes in coal mines and to induce rockburst. Based on the“key strata”theory, the integrated spatial structure of overlying strata was put forward, which was composed of “O-X” structure in the plane section and “F” structure in the vertical section. The formation and ongoing instability of the“O-X”and“F”structures were called as dynamic evolution cycle of the overlying strata. Three basic categories of “O-X”, “F” and “T” structures were defined, and the strata behaviors of each spatial structure were analyzed. According to energy theory, mechanism of rockburst induced by spatial structure instability was discussed. The research expanded the scope of traditional ground pressure theory and provided a guide for the prevention of rockburst and mining tremors induced by structure instability of overlying
基金TheDevelopingFoundationofShanghaiScienceandTechnologyCommittee (No .9944 190 2 7) .
文摘A positioning volume ellipsoid method is proposed to represent tumor volume in the workspace of a high-intensity-focused ultrasound (HIFU) surgery platform during the platform's positioning motion. To this simplified tumor model, a nearest neighbor search method is used to determine intermediate configuration and goal configuration, which the treatment head and ultrasound focus must pass in their localization to target volume. Based on the decided configurations, the continuity condition of combined Bezier curve in Euclidean space and De Casteljau algorithm on Lie group SO(3) are integrated to construct a combined Bezier positioning path of C2 continuity at junctional configuration, and an illustration of different positioning path planning is analyzed based on a liver tumor case.
文摘A constrained partial permutation strategy is proposed for matching spatial relation graph (SRG), which is used in our sketch input and recognition system Smart Sketchpad for representing the spatial relationship among the components of a graphic object. Using two kinds of matching constraints dynamically generated in the matching process, the proposed approach can prune most improper mappings between SRGs during the matching process. According to our theoretical analysis in this paper, the time complexity of our approach is O(n 2) in the best case, and O(n!) in the worst case, which occurs infrequently. The spatial complexity is always O(n) for all cases. Implemented in Smart Sketchpad, our proposed strategy is of good performance.
