期刊文献+

二次检索

题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到2,261篇文章
< 1 2 114 >
每页显示 20 50 100
Subconjunctival trypsin injection for anterior chamber fibrin exudates in eyes with globe rupture following vitrectomy
1
作者 Shu-Wen Lu Hao-Yu Li +3 位作者 Xin-Min Li Chao Ma Xian Li Qiu-Ming Hu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第11期2037-2044,共8页
AIM:To compare the safety and clinical outcomes of subconjunctival trypsin and dexamethasone(DEX)injections in the treatment of anterior chamber fibrin exudates in eyes with globe rupture following primary wound repai... AIM:To compare the safety and clinical outcomes of subconjunctival trypsin and dexamethasone(DEX)injections in the treatment of anterior chamber fibrin exudates in eyes with globe rupture following primary wound repair and vitrectomy.METHODS:A retrospective analysis included 42 males and 10 females(mean age 46.0±6.0y,range 34 to 58y)who underwent primary wound sutures and vitrectomy for globe rupture.Patients with pupil-covered fibrinous exudate or/and membrane in the anterior chamber were treated.On the first postoperative day,subconjunctival injections of either 5000 units(0.4 mL)of trypsin solution(n=25)or 0.5 mL(1 mg)DEX(n=27)were administered to accelerate exudate absorption.Efficacy was assessed by observing break time and partial absorption of the fibrin exudate membrane.Safety and comfort were evaluated by monitoring intraocular pressure(IOP),allergy,pain,and foreign body sensation.RESULTS:Both groups achieved 1/3 absorption of the anterior chamber fibrin exudate membrane,but the trypsin group exhibited shorter break time and partial absorption time compared to the DEX group(P<0.05).Trypsin treatment was also less irritating to patients.No adverse reactions were reported,and IOP remained stable.Visual acuity improved in both groups without statistical difference.CONCLUSION:Compared to DEX,trypsin demonstrates a shorter absorption time for the fibrin exudate membrane with a more comfortable process in treating pupil-covered fibrinous exudate or/and membrane after vitrectomy for globe rupture. 展开更多
关键词 globe rupture VITRECTOMY exudative fibrin membrane TRYPSIN dexamethasone
下载PDF
Therapeutic Effectiveness of Leukocyte-and Platelet-rich Fibrin for Diabetic Foot Ulcers:A Retrospective Study
2
作者 Fen WANG Xiao-ling ZHANG +9 位作者 Jing ZHANG Song GONG Jing TAO Hui XIANG Xiao-qing FU Xu-na BIAN Xue-feng YU An-hui XU Cheng-la YI Shi-ying SHAO 《Current Medical Science》 SCIE CAS 2024年第3期568-577,共10页
Objective Diabetic foot ulcer(DFU)is one of the most serious complications of diabetes.Leukocyte-and platelet-rich fibrin(L-PRF)is a second-generation autologous platelet-rich plasma.This study aims to investigate the... Objective Diabetic foot ulcer(DFU)is one of the most serious complications of diabetes.Leukocyte-and platelet-rich fibrin(L-PRF)is a second-generation autologous platelet-rich plasma.This study aims to investigate the clinical effects of L-PRF in patients with diabetes in real clinical practice.Methods Patients with DFU who received L-PRF treatment and standard of care(SOC)from 2018 to 2019 in Tongji Hospital were enrolled.The clinical information including patient characteristics,wound evaluation(area,severity,infection,blood supply),SOC of DFU,and images of ulcers was retrospectively extracted and analyzed.L-PRF treatment was performed every 7±2 days until the ulcer exhibited complete epithelialization or an overall percent volume reduction(PVR)greater than 80%.Therapeutic effectiveness,including overall PVR and the overall and weekly healing rates,was evaluated.Results Totally,26 patients with DFU were enrolled,and they had an ulcer duration of 47.0(35.0,72.3)days.The severity and infection of ulcers varied,as indicated by the Site,Ischemia,Neuropathy,Bacterial Infection,and Depth(SINBAD)scores of 2–6,Wagner grades of 1–4,and the Perfusion,Extent,Depth,Infection and Sensation(PEDIS)scores of 2–4.The initial ulcer volume before L-PRF treatment was 4.94(1.50,13.83)cm3,and the final ulcer volume was 0.35(0.03,1.76)cm3.The median number of L-PRF doses was 3(2,5).A total of 11 patients achieved complete epithelialization after the fifth week of treatment,and 19 patients achieved at least an 80%volume reduction after the seventh week.The overall wound-healing rate was 1.47(0.63,3.29)cm3/week,and the healing rate was faster in the first 2 weeks than in the remaining weeks.Concurrent treatment did not change the percentage of complete epithelialization or healing rate.Conclusion Adding L-PRF to SOC significantly improved wound healing in patients with DFU independent of the ankle brachial index,SINBAD score,or Wagner grade,indicating that this method is appropriate for DFU treatment under different clinical conditions. 展开更多
关键词 diabetic foot ulcer autologous platelet-rich plasma leukocyte-and platelet-rich fibrin standard of care percentage volume reduction
下载PDF
Current Research Progress of Central Venous Catheter-Associated Fibrin Sheath Prevention and Nursing Care
3
作者 Linzhi Xu Yue Li Hongjuan Wu 《Journal of Clinical and Nursing Research》 2024年第5期135-144,共10页
With the popularization of central venous catheterization in recent years,the problems arising from intravenous therapy have gradually increased.Fibrin sheath is the complication with the highest incidence rate in cen... With the popularization of central venous catheterization in recent years,the problems arising from intravenous therapy have gradually increased.Fibrin sheath is the complication with the highest incidence rate in central venous catheterization,which has always been a major problem in intravenous therapy.So the prevention and treatment of fibrin sheath has become a hot spot of research in recent years.Hence,this paper summarizes the research on fibrin sheath in recent years. 展开更多
关键词 Central venous catheterization fibrin sheath Clinical care
下载PDF
Adult-Onset Still's Disease Misdiagnosed as Acute Fibrinous and Organizing Pneumonia: A Case Report and Literature Review
4
作者 Chunhua Huang Chunting Lai 《Journal of Clinical and Nursing Research》 2024年第6期90-93,共4页
Adult-onset Stil's disease(AOSD)is a rare condition that lies between autoinflammatory syndrome and autoimmune disease.The main clinical manifestations include fever,chills,rash,joint swelling and pain,peripheral ... Adult-onset Stil's disease(AOSD)is a rare condition that lies between autoinflammatory syndrome and autoimmune disease.The main clinical manifestations include fever,chills,rash,joint swelling and pain,peripheral blood leukocytosis,splenomegaly,etc.It is a systemic disease affecting between 1 and 34 people per million.The average age of onset is 35 years old,with a slightly higher prevalence rate in women.Since AOSD lacks early specific symptoms and signs,non-specialist doctors have limited understanding of the disease,and patients are prone to clinical misdiagnosis,mistreatment,and delayed disease progression.This paper reports a patient whose AOSD was misdiagnosed as acute fibrinous and organizing pneumonia. 展开更多
关键词 Adult-onset Still's disease Acute fibrinous and organizing pneumonia Lung disease
下载PDF
Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane 被引量:4
5
作者 Shengzhong Ma Changliang Peng +2 位作者 Shiqing Wu Dongjin Wu Chunzheng Gao 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第36期3416-3422,共7页
Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration; however, the precise mechanism remains unclear, p75 neur... Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration; however, the precise mechanism remains unclear, p75 neurotrophin receptor (p75NTR) plays an important role in the regulation of peripheral nerve regeneration. We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75NTR expression. In this study, we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve. Through RT-PCR and western blot analysis, nerve growth factor-containing fibrin glue membrane significantly increased p75NTR mRNA and protein expression in the Schwann cells at the anastomotic site, in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture. These results indicate that nerve growth factor-containing fibrin glue membrane can promote peripheral nerve regeneration by up-regulating p75NTR expression in Schwann cells. 展开更多
关键词 neural regeneration nerve growth factor-containing fibrin gluereceptor Schwann cells peripheral nerve regeneration fibrin gluemembrane p75 neurotrophinnerve growth factor peripheralnerve injury sciatic nerve microenvironment grants-supported paper NEUROREGENERATION
下载PDF
Correlation of fibrinogen-like protein 2 with progression of acute pancreatitis in rats 被引量:4
6
作者 Xiao-Hua Ye Tan-Zhou Chen +6 位作者 Jia-Ping Huai Guang-Rong Lu Xiao-Ju Zhuge Ren-Pin Chen Wu-Jie Chen Chen Wang Zhi-Ming Huang 《World Journal of Gastroenterology》 SCIE CAS 2013年第16期2492-2500,共9页
AIM: To examine fibrinogen-like protein 2 (fgl2) expression during taurocholate-induced acute pancreatitis progression in rats and its correlation with pancreatic injury severity. METHODS: Forty-eight male Sprague-Daw... AIM: To examine fibrinogen-like protein 2 (fgl2) expression during taurocholate-induced acute pancreatitis progression in rats and its correlation with pancreatic injury severity. METHODS: Forty-eight male Sprague-Dawley rats were randomly divided into the severe acute pancreatitis (SAP) group (n = 24) and the sham operation (SO) group (n = 24). Sodium taurocholate (4% at doses of 1 mL/kg body weight) was retrogradely injected into the biliopancreatic ducts of the rats to induce SAP. Pancreatic tissues were prepared immediately after sacrifice. At the time of sacrifice, blood was obtained for determination of serum amylase activity and isolation of peripheral blood mononuclear cells (PBMCs). Pancreatic tissue specimens were obtained for routine light microscopy including hematoxylin and eosin staining, and the severity of pancreatic injury was evaluated 1, 4 and 8 h after induction. Expression of fgl2 mRNA was measured in the pancreas and PBMCs using reverse transcription polymerase chain reaction. Expression of fgl2 protein was evaluated in pancreatic tissues using Western blotting and immunohistochemical staining. Masson staining was also performed to observe microthrombosis. RESULTS: At each time point, levels of fgl2 mRNAs in pancreatic tissues and PBMCs were higher (P < 0.05) in the SAP group than in the SO group. For pancreatic tissue in SAP vs SO, the levels were: after 1 h, 3.911 ± 1.277 vs 1.000 ± 0.673; after 4 h, 9.850 ± 3.095 vs 1.136 ± 0.609; and after 8 h, 12.870 ± 3.046 vs 1.177 ± 0.458. For PBMCs in SAP vs SO, the levels were: after 1 h, 2.678 ± 1.509 vs 1.000 ± 0.965; after 4 h, 6.922 ± 1.984 vs 1.051 ± 0.781; and after 8 h, 13.533 ± 6.575 vs 1.306 ± 1.179. Levels of fgl2 protein expression as determined by Western blotting and immunohistochemical staining were markedly up-regulated (P < 0.001) in the SAP group compared with those in the SO group. For Western blotting in SAP vs SO, the results were: after 1 h, 2.183 ± 0.115 vs 1.110 ± 0.158; after 4 h, 2.697 ± 0.090 vs 0.947 ± 0.361; and after 8 h, 3.258 ± 0.094 vs 1.208 ± 0.082. For immunohistochemical staining in SAP vs SO, the results were: after 1 h, 1.793 ± 0.463 vs 0.808 ± 0.252; after 4 h, 4.535 ± 0.550 vs 0.871 ± 0.318; and after 8 h, 6.071 ± 0.941 vs 1.020 ± 0.406. Moreover, we observed a positive correlation in the pancreas (r = 0.852, P < 0.001) and PBMCs (r = 0.735, P < 0.001) between fgl2 expression and the severity of pancreatic injury. Masson staining showed that microthrombosis (%) in rats with SAP was increased (P < 0.001) compared with that in the SO group and it was closely correlated with fgl2 expression in the pancreas (r = 0.842, P < 0.001). For Masson staining in SAP vs SO, the results were: after 1 h, 26.880 ± 9.031 vs 8.630 ± 3.739; after 4 h, 53.750 ± 19.039 vs 8.500 ± 4.472; and after 8 h, 80.250 ± 12.915 vs 10.630 ± 7.003.CONCLUSION: Microthrombosis due to fgl2 overexpression contributes to pancreatic impairment in rats with SAP, and fgl2 level may serve as a biomarker during early stages of disease. 展开更多
关键词 fibrinogen-like PROTEIN 2 MICROTHROMBOSIS fibrin Severe acute pancreatitis Peripheral blood MONONUCLEAR cell
下载PDF
VASCULAR ENDOTHELIAL INJURIES AND CHANGES OF BLOOD COAGULATION AND FIBRINOLYSIS INDEXES IN PATIENTS WITH ACUTE RESPIRATORY DISTRESS SYNDROME 被引量:4
7
作者 Xiao-linHe ZhiLiu Shu-yueXia 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第4期252-256,共5页
Objective To study endothelial damage by observing changes of circulating endothelial cells (CECs) in blood, coagula-tion and fibrinolysis index in patients with acute respiratory distress syndrome. Methods CECs were ... Objective To study endothelial damage by observing changes of circulating endothelial cells (CECs) in blood, coagula-tion and fibrinolysis index in patients with acute respiratory distress syndrome. Methods CECs were separated by isopycnic centrifugation method in 14 patients with acute lung injury (ALI), 7 patients with acute respiratory distress syndrome (ARDS), 10 intensive care unit (ICU) controls, and 15 healthy controls. Plasma prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FG), fibrin degradation products (FDP), and D-dimer were examined simultaneously. Acute physiology and chronic health evaluation (APACHE)Ⅱand lung injury score (LIS) were recorded to evaluate severity of illness and lung injury. Results (1) The number of CECs in ALI (10.4 ±2.3) and ARDS groups (16.1 ±2.7) was higher than that in the healthy (1.9 ±0.5) (P< 0.01). In both ALI and ARDS, the number of CECs correlated with APACHEⅡ(r=0.55, P< 0.05 and r=0.62, P< 0.05, respectively)and LIS (r=0.60, P< 0.05 and r=0.53, P< 0.05, respectively). CEC number was negatively correlated with PaO 2 in ALI and ARDS (r=-0.49, P< 0.05 and r=-0.64, P< 0.05, respectively). (2) The level of FDP and D-dimer were higher in ALI and ARDS patients than that in ICU and healthy control groups (P< 0.05). The level of FG in ARDS group was significantly higher than in the ICU and healthy control groups (P< 0.05). But in ALI group, the level of FG was significantly higher than only healthy control group (P< 0.05). Conclusions Endothelial cell damage occurs in ARDS patients, which may play a major role in the pathophysiology of ARDS. Changes of endothelial cell activation and damage markers, such as CECs, plasma coagulation and fibrinolysis index, to some extent reflect severity of illness and lung injury in ARDS. 展开更多
关键词 acute respiratory distress syndrome endothelial cell fibrinOGEN fibrin D-DIMER
下载PDF
独活寄生汤含药血清对PLGA/Fibrin复合支架培养兔髓核细胞代谢影响的研究 被引量:2
8
作者 杨洋 徐卫星 +5 位作者 俞雷钧 王健 宋红浦 刘建 范顺武 辛龙 《现代实用医学》 2019年第7期910-911,992,I0002,共4页
目的探讨独活寄生汤含药血清对兔髓核细胞在PLGA/Fibrin复合支架培养代谢的影响。方法应用冻干法制备PLGA/Fibrin支架培养兔髓核细胞,按照不同浓度独活寄生汤含药血清分为A组(100μg/ml)、B组(200μg/ml)、C组(500μg/ml)及D组(空白对照... 目的探讨独活寄生汤含药血清对兔髓核细胞在PLGA/Fibrin复合支架培养代谢的影响。方法应用冻干法制备PLGA/Fibrin支架培养兔髓核细胞,按照不同浓度独活寄生汤含药血清分为A组(100μg/ml)、B组(200μg/ml)、C组(500μg/ml)及D组(空白对照)。通过MTT分析细胞增殖分化,DMMB法检测硫酸糖胺多糖(GAG),qRT-PCR检测COLⅡ、aggrecan差异,组织学观察髓核细胞在PLGA/Fibrin复合支架微孔环境生长形态学改变。结果培养1、7及14d后A、B、C3组髓核细胞活性、GAG水平、COLⅡ及Aggrecan mRNA表达均高于D组,且随着独活寄生汤含药血清浓度递增,细胞活性及基质表达有显著增加。培养2周后HE染色显示A、B、C3组较D组微孔支架吸附、积聚更多髓核细胞生长。结论PLGA/Fibrin复合支架能满足兔髓核细胞的黏附、伸展及繁殖要求,并为维持细胞的体外增殖和代谢活动提供适宜的微环境。独活寄生汤能够更好提高髓核细胞分化增殖能力,促进细胞基质分泌,从而减缓了椎间盘的退变。 展开更多
关键词 独活寄生汤 PLGA/fibrin复合支架 兔髓核细胞 细胞培养
下载PDF
Using antifibrinolytics to tackle neuroinflammation 被引量:1
9
作者 Stanimir Atsev Nikola Tomov 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第12期2203-2206,共4页
Plasmin is generally known as a promotor of inflammation.Recent advancement suggests that it has a complex role as immunity modulator.Pharmacological inhibition of plasmin production and activity has been proven to im... Plasmin is generally known as a promotor of inflammation.Recent advancement suggests that it has a complex role as immunity modulator.Pharmacological inhibition of plasmin production and activity has been proven to improve neurological outcomes in traumatic brain injury and subarachnoid hemorrhage,most probably by preventing re-bleeding.The immune-modulatory properties of antifibrinolytics,however,suggest that they probably have effects unrelated to fibrinolysis inhibition,which are currently not adequately harnessed.The present work aims to give an account of the existing data regarding antifibrinolytics as agents influencing neuroinflammation.Preclinical and clinical studies on the possible influence of antifibrinolytics on neuroinflammation are scarce.However,the emerging evidence suggests that inhibition of plasmin(ogen)activity can ameliorate neuroinflammation to some extent.This data demonstrate that plasmin(ogen)is not exclusively involved in fibrinolysis,but also has other substrates and can precipitate in inflammatory processes.Investigation on the role of plasmin as the factor for the development of neuroinflammation shows the significant potential of antifibrinolytics as pharmacotherapy of neuroinflammationm,which is worthy of further exploration. 展开更多
关键词 antifibrionolytics fibrin fibrinogen NEUROINFLAMMATION PLASMIN PLASMINOGEN tranexamic acid
下载PDF
PREPARATION AND CHARACTERIZATION OF TWO MONOCLONAL ANTIBODIES AGAINST A_α CHAIN'S C TERMINUS OF FIBRINOGEN
10
作者 阮长耿 《中国实验血液学杂志》 CAS CSCD 1995年第1期91-95,共5页
Two monoclonal antibodies (McAbs) against Aα chain's C terminus offibrinogen (Fg) have been prepared and designated SZ-78 and SZ-79. Both theantigens in binding assay and immunoblot analysis showed that the two M... Two monoclonal antibodies (McAbs) against Aα chain's C terminus offibrinogen (Fg) have been prepared and designated SZ-78 and SZ-79. Both theantigens in binding assay and immunoblot analysis showed that the two McAbs recognized the epitopes located in residues 549-560 of the Aαchain. The two McAbs couldaccelerate rate of fibrin polymer assembly both in the purified system and in the humanplasma. From the pictures of transmission electronmicroscope, the average diametersof the fibers increase significantly to an average diameters of 375 nm after incubationwith the McAbs, while it was only 75nm without addition of the McAbs. There were al-so more branchings of fibers with addition of McAbs. These observations demonstratethat the amino acid sequences ofα 549-560 in the COOH terminus of the Aα chain mayplay an important role in the assembly of a fibrin clot, presumably being involved in lat-eral aggregation of protofibrils. The preparation of the McAbs supplies a usuful probe for the investigation of the 展开更多
关键词 MONOCLONAL antibody polymerization fibrin Aαchain
下载PDF
Two methods of lung biopsy for histological confirmation of acute fibrinous and organizing pneumonia:A case report
11
作者 Wen-Juan Liu Shuang Zhou Yan-Xia Li 《World Journal of Clinical Cases》 SCIE 2022年第36期13381-13387,共7页
BACKGROUND Acute fibrinous and organizing pneumonia(AFOP)is a rare,noninfective lung disease,histologically characterized by a patchy distribution of intra-alveolar fibrin“balls”and organizing pneumonia.The clinical... BACKGROUND Acute fibrinous and organizing pneumonia(AFOP)is a rare,noninfective lung disease,histologically characterized by a patchy distribution of intra-alveolar fibrin“balls”and organizing pneumonia.The clinical manifestations of AFOP are nonspecific.Diagnosis depends on pathology.Surgical lung biopsy is optimal for tissue sampling to diagnose AFOP.However,many patients have no tolerance to the operation,including mentally and physically.There is still no standard therapy for AFOP and the methods remain controversial.Therefore,further clinical attention and discussion are warranted.CASE SUMMARY A 53-year-old woman presented with fever,cough and dyspnea for 15 d.Antiinfective therapy was ineffective.Chest computed tomography showed bilateral patchy consolidation,especially in the lower lobes.We performed both ultrasound-guided transbronchial lung biopsy and ultrasound-guided percutaneous fine needle puncture at different lung lesion locations.Both samples supported the diagnosis of AFOP.The patient had a good clinical course after treatment with methylprednisolone,and no side effects of steroids.CONCLUSION Percutaneous needle biopsy combined with transbronchial lung biopsies may be a good choice in the absence of surgical biopsy.Methylprednisolone alone is effective in the treatment of idiopathic AFOP. 展开更多
关键词 Acute fibrinous and organizing pneumonia fibrin balls Percutaneous needle biopsy Transbronchial lung biopsies METHYLPREDNISOLONE Case report
下载PDF
PLGA-Fibrin杂合支架构建及与脂肪源性干细胞复合成软骨的研究 被引量:4
12
作者 王君 周琦 +2 位作者 邓廉夫 胡蕴玉 魏义勇 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2010年第8期758-760,共3页
目的:探讨在特定的软骨细胞诱导培养液中,由纤维蛋白(fibrin)修饰的聚乳酸/聚羟基乙酸共聚物(PLGA)杂合支架材料对脂肪源性干细胞成软骨的影响。方法:PLGA支架材料采用快速成形技术制备;PLGA-Fibrin杂和支架材料采用低温冷冻... 目的:探讨在特定的软骨细胞诱导培养液中,由纤维蛋白(fibrin)修饰的聚乳酸/聚羟基乙酸共聚物(PLGA)杂合支架材料对脂肪源性干细胞成软骨的影响。方法:PLGA支架材料采用快速成形技术制备;PLGA-Fibrin杂和支架材料采用低温冷冻的方法制备。分离兔脂肪源性干细胞,分别种植在未修饰的PLGA支架材料和Fbirin修饰的PLGA上,并在特定软骨诱导液中培养至14d,分别应用SEM观察杂合支架材料的性能;生化方法检测硫酸软骨素的含量;支架材料的亲水性和细胞黏附能力也被检测。通过体内实验对两种支架材料与干细胞复合修复软骨缺损的性能进行形态学染色和评分。结果:体外研究表明,与未修饰的PLGA支架材料相比,Fibfn修饰的PLGA亲水性明显被提高,同时也提高了干细胞在杂合支架材料上的黏附性能和硫酸软骨素的含量。软骨损伤修复研究表明,脂肪源性干细胞与Fibirn修饰的PLGA支架材料复合后,具有良好的修复软骨缺损的功能,其性能明显优于单纯的PLGA材料复合干细胞。结论:Fibim修饰的PLGA支架材料可提高脂肪源性干细胞向软骨细胞分化的能力,促进缺损软骨的再生。 展开更多
关键词 脂肪源性干细胞 软骨 纤维蛋白 PLGA
下载PDF
Platelet-rich fibrin-induced bone marrow mesenchymal stem cell differentiation into osteoblast-like cells and neural cells 被引量:12
13
作者 Qi Li Yajun Geng +3 位作者 Lei Lu Tingting Yang Mingrui Zhang Yanmin Zhou 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第31期2419-2423,共5页
Bone marrow mesenchymal stem cells were allowed to develop for 14 days in a platelet-rich fibrin environment.Results demonstrated that platelet-rich fibrin significantly promoted bone marrow mesenchymal stem cell prol... Bone marrow mesenchymal stem cells were allowed to develop for 14 days in a platelet-rich fibrin environment.Results demonstrated that platelet-rich fibrin significantly promoted bone marrow mesenchymal stem cell proliferation.In addition,there was a dose-dependent increase in Runt-related transcription factor-2 and bone morphogenetic protein-2 mRNA expression,as well as neuron-specific enolase and glial acidic protein.Results showed that platelet-rich fibrin promoted bone marrow mesenchymal stem cell proliferation and differentiation of osteoblast-like cells and neural cells in a dose-dependent manner. 展开更多
关键词 alkaline phosphatase bone marrow mesenchymal stem cells glial fibrillary acidic protein neural regeneration neuron-specific enolase platelet-rich fibrin
下载PDF
Reduction of the closure time of postoperative enterocutaneous fistulas with fibrin sealant 被引量:10
14
作者 Jorge Avalos-González Eliseo Portilla-deBuen +7 位作者 Caridad Aurea Leal-Cortés Abel Orozco-Mosqueda María del Carmen Estrada-Aguilar Gabriela Abigail Velázquez-Ramírez Gabriela Ambriz-González Clotilde Fuentes-Orozco Aldo Emmerson Guzmán-Gurrola Alejandro González-Ojeda 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第22期2793-2800,共8页
AIM: To assess whether the use of fibrin sealantshortens the closure time of postoperative enterocutaneous fistulas (ECFs). METHODS: The prospective case-control study included 70 patients with postoperative ECFs with... AIM: To assess whether the use of fibrin sealantshortens the closure time of postoperative enterocutaneous fistulas (ECFs). METHODS: The prospective case-control study included 70 patients with postoperative ECFs with an output of < 500 mL/d, a fistulous tract of > 2 cm and without any local complication. They were divided into study (n = 23) and control groups (n = 47). Esophageal, gastric and colocutaneous fistulas were monitored under endoscopic visualization, which also allowed fibrin glue application directly through the external hole. Outcome variables included closure time, time to resume oral feeding and morbidity related to nutritional support. RESULTS: There were no differences in mean age, fistula output, and follow-up. Closure-time for all patients of the study group was 12.5 ± 14.2 d and 32.5 ± 17.9 d for the control group (P < 0.001), and morbidity related to nutritional support was 8.6% and 42.5%, respectively (P < 0.01). In patients with colonic fistulas, complete closure occurred 23.5 ± 19.5 d after the first application of fibrin glue, and spontaneous closure was observed after 36.2 ± 22.8 d in the control group (P = 0.36). Recurrences were observed in 2 patients because of residual disease. One patient of each group died during follow-up as a consequence of septic complications related to parenteral nutrition. CONCLUSION: Closure time was significantly reduced with the use of fibrin sealant, and oral feeding was resumed faster. We suggest the use of fibrin sealant for the management of stable enterocutaneous fistulas. 展开更多
关键词 Enterocutaneous fistulas fibrin sealant Spontaneous closure
下载PDF
Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation 被引量:15
15
作者 Mengge Feng Yulan Wang +5 位作者 Peng Zhang Qin Zhao Shimin Yu Kailun Shen Richard J.Miron Yufeng Zhang 《International Journal of Oral Science》 SCIE CAS CSCD 2020年第4期309-316,共8页
Platelet-rich fibrin(PRF)has been widely used owing to its ability to stimulate tissue regeneration.To date,few studies have described the antibacterial properties of PRF.Previously,PRF prepared by horizontal centrifu... Platelet-rich fibrin(PRF)has been widely used owing to its ability to stimulate tissue regeneration.To date,few studies have described the antibacterial properties of PRF.Previously,PRF prepared by horizontal centrifugation(H-PRF)was shown to contain more immune cells than leukocyte-and platelet-rich fibrin(L-PRF).This study aimed to compare the antimicrobial effects of PRFs against Staphylococcus aureus and Escherichia coli in vitro and to determine whether the antibacterial effects correlated with the number of immune cells.Blood samples were obtained from eight healthy donors to prepare L-PRF and H-PRF.The sizes and weights of L-PRF and H-PRF were first evaluated,and their antibacterial effects against S.aureus and E.coli were then tested in vitro using the inhibition ring and plate-counting test methods.Flow-cytometric analysis of the cell components of L-PRF and H-PRF was also performed.No significant differences in size or weight were observed between the L-PRF and H-PRF groups.The H-PRF group contained more leukocytes than the L-PRF group.While both PRFs had notable antimicrobial activity against S.aureus and E.coli,H-PRF demonstrated a significantly better antibacterial effect than L-PRF.Furthermore,the antimicrobial ability of the PRF solid was less efficient than that of wet PRF.In conclusion,H-PRF exhibited better antibacterial activity than L-PRF,which might have been attributed to having more immune cells. 展开更多
关键词 fibrin BLOOD PLATELET
下载PDF
Effect of implanting fibrin sealant with ropivacaine on pain after laparoscopic cholecystectomy 被引量:8
16
作者 Jian-Zhu Fu Jie Li Ze-Li Yu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第46期5851-5854,共4页
AIM:To investigate the safety and efficacy of implant-ing fibrin sealant with sustained-release ropivacaine in the gallbladder bed for pain after laparoscopic chole-cystectomy(LC).METHODS:Sixty patients(American Socie... AIM:To investigate the safety and efficacy of implant-ing fibrin sealant with sustained-release ropivacaine in the gallbladder bed for pain after laparoscopic chole-cystectomy(LC).METHODS:Sixty patients(American Society of Anes-thesiologists physical status wasⅠorⅡand underwent LC)were randomly divided into three equal groups:group A(implantation of fibrin sealant in the gallbladder bed),group B(implantation of fibrin sealant carrying ropivacaine in the gallbladder bed),and group C(normal saline in the gallbladder bed).Postoperative pain was evaluated,and pain relief was assessed by visual analog scale(VAS)scoring.RESULTS:The findings showed that 81.7%of patients had visceral pain,50%experienced parietal,and 26.7% reported shoulder pain after LC.Visceral pain was significantly less in group B patients than in the other groups(P<0.05),and only one patient in this group experienced shoulder pain.The mean VAS score in group B patients was lower than that in the other groups.CONCLUSION:Visceral pain is prominent after LC and can be effectively controlled by implanting fibrin sealant combined with ropivacaine in the gallbladder bed. 展开更多
关键词 ANALGESIA fibrin sealant Laparoscopiccholecystectomy PAIN ROPIVACAINE
下载PDF
Fibrin sealant for closure of mucosal penetration at the cardia during peroral endoscopic myotomy: A retrospective study at a single center 被引量:11
17
作者 Wen-Gang Zhang En-Qiang Linghu Hui-Kai Li 《World Journal of Gastroenterology》 SCIE CAS 2017年第9期1637-1644,共8页
AIM To assess the efficacy and safety of fibrin sealant for closure of mucosal penetration at the cardia during peroral endoscopic myotomy(POEM).METHODS Twenty-four patients who underwent POEM and experienced mucosal ... AIM To assess the efficacy and safety of fibrin sealant for closure of mucosal penetration at the cardia during peroral endoscopic myotomy(POEM).METHODS Twenty-four patients who underwent POEM and experienced mucosal injury of the cardia during the procedure were retrospectively identified. Of the 24 patients, 21 had mucosal penetration and 3 had only slight mucosal damage without penetration. The 21 patients with mucosal penetration received fibrin sealant for closure at the site of penetration. Penetration-related characteristics, treatment, and recovery were reviewed for all 21 patients to assess the efficacy and safety of fibrin sealant for closure of mucosal penetration at the cardia. Clinical data, including general characteristics, procedure-related parameters, Eckardt scores, lower esophageal sphincter pressures (LESP), and esophagogastroduodenoscopy(EGD) results, were analyzed to determine their influence on treatment success after mucosal penetration during POEM.RESULTS All 21 patients had a solitary mucosal penetration in the cardia (12 in esophageal region of the cardia, 9 in the stomach region of the cardia, and 1 in both the esophageal and stomach regions). Twelve had ahole-like penetration and 9 had a linear penetration. For those with a hole-like penetration, the mean size was 0.14 cm2 (0.02-0.32 cm2). For those with a linear penetration, the median size was 0.37 cm (0.10-1.00 cm). Closure of the mucosal penetration using fibrin sealant was performed successfully in all 21 patients(two patients required 5 m L fibrin sealant, and the remaining 19 patients required 2.5 mL). Two patients had a nasogastric tube placed for five days after POEM; the remaining 19 patients were kept fasting for 3 d. All 21 patients were discharged after a median of 5 d(range: 5-7 d) postoperatively. During a median 42 mo (range: 9-62 mo) follow-up, all 21 patients with a mucosal penetration successfully healed without the occurrence of infection, ulcer, or esophagitis. Furthermore, the median LESP decreased from 31.9 mm Hg (range: 21.9-67.1 mm Hg) preoperatively to 20.3 mm Hg (range: 6.0-41.0 mm Hg) postoperatively(P < 0.05). The median preoperative and postoperative Eckardt scores were 5.0(range; 4-10) and 1.0(range: 0-4), respectively (P < 0.05). Of the 21 patients with mucosal penetration, symptom remission, which is defined as a postoperative Eckardt score ≤ 3, was achieved in 20 patients(95.2%) indicating that mucosal penetration did not influence the success of POEM treatment if closed successfully using fibrin sealant.CONCLUSION Fibrin sealant is safe and effective for closure of mucosal penetration during POEM. Mucosal penetrations do not appear to influence the treatment success of POEM if closed successfully using fibrin sealant. Additional studies regarding the feasibility, efficacy, and safety of fibrin sealant for closure of larger mucosal penetrations is warranted. 展开更多
关键词 fibrin sealant Mucosal penetration Peroral endoscopic myotomy EFFICACY Safety
下载PDF
Periodontally accelerated osteogenic orthodontics with platelet-rich fibrin in an adult patient with periodontal disease:A case report and review of literature 被引量:9
18
作者 Min Xu Xiao-Yu Sun Jian-Guang Xu 《World Journal of Clinical Cases》 SCIE 2021年第6期1367-1378,共12页
BACKGROUND In the clinical scenario,adult patients with periodontal diseases and dental malformation,characterized by dental crowding in lower anterior teeth with the thin biotype,often require orthodontic treatment.T... BACKGROUND In the clinical scenario,adult patients with periodontal diseases and dental malformation,characterized by dental crowding in lower anterior teeth with the thin biotype,often require orthodontic treatment.This case report aimed to evaluate the clinical and radiographic outcomes of periodontally accelerated osteogenic orthodontics(PAOO)combined with autologous platelet-rich fibrin(PRF)in an adult patient with class I malocclusion along with dental crowding,a thin periodontal biotype,and buccal plate deficiency.CASE SUMMARY A 32-year-old female complaining of dental crowding and gingival bleeding was referred to the orthodontic clinic.The patient underwent periodontal risk assessment prior to orthodontic treatment.She was diagnosed with a high risk of gingival recession due to dental crowding,root prominence,loss of buccal plates,and a thin gingival tissue biotype.The treatment regimen included PAOO combined with autologous PRF for alveolar augmentation and interproximal enamel reduction for moderate dental crowding.Clinically,PAOO-assisted orthodontic tooth movement in this case showed enhanced periodontium remodeling.Radiographic outcomes also showed statistically significant improvements(P<0.01)in the mandibular buccal alveolar bone.CONCLUSION This case report suggests the combination of autologous PRF with PAOO to enhance bone augmentation and long-term tissue support in adult orthodontic patients with periodontal disease. 展开更多
关键词 Periodontally accelerated osteogenic orthodontics Orthodontic tooth movement Platelet-rich fibrin Bone augmentation Periodontal biotype Case report
下载PDF
possible role of soluble fibrin monomer complex after gastroenterological surgery 被引量:3
19
作者 Masatoshi Kochi Manabu Shimomura +6 位作者 Takao Hinoi Hiroyuki Egi Kazuaki Tanabe Yasuyo Ishizaki Tomohiro Adachi Hirotaka Tashiro Hideki Ohdan 《World Journal of Gastroenterology》 SCIE CAS 2017年第12期2209-2216,共8页
AIM To examine the role of soluble fibrin monomer complex(SFMC) in the prediction of hypercoagulable state after gastroenterological surgery.METHODS We collected data on the clinical risk factors and fibrin-related ma... AIM To examine the role of soluble fibrin monomer complex(SFMC) in the prediction of hypercoagulable state after gastroenterological surgery.METHODS We collected data on the clinical risk factors and fibrin-related makers from patients who underwent gastroenterological surgery at Hiroshima University Hospital between April 1, 2014 and March 31, 2015. We investigated the clinical significance of SFMC, which is known to reflect the early plasmatic activation of coagulation, in the view of these fibrin related markers.RESULTS A total of 123 patients were included in the present study. There were no patients with symptomatic VTE. Thirty-five(28%) patients received postoperative anticoagulant therapy. In the multivariate analysis, a high SFMC level on POD 1 was independently associated with D-dimer elevation on POD 7(OR = 4.31, 95%CI: 1.10-18.30, P = 0.03). The cutoff SFMC level was 3.8 μg/ml(AUC = 0.78, sensitivity, 63%, specificity, 89%). The D-dimer level on POD 7 was significantly reduced in high-SFMC patients who received anticoagulant therapy in comparison to highSFMC patients who did not.CONCLUSION The SFMC on POD 1 strongly predicted the hypercoagulable state after gastroenterological surgery than the clinical risk factors and the other fibrin related markers. 展开更多
关键词 Hypercoagulable state Gastroenterological surgery Soluble fibrin monomer complex Venous thromboembolism Anticoagulant therapy
下载PDF
Common bile duct injury by fibrin glue: Report of a rare complication 被引量:2
20
作者 Yu-Long Yang Cheng Zhang +6 位作者 Hong-Wei Zhang Ping Wu Yue-Feng Ma Mei-Ju Lin Li-Jun Shi Jing-Yi Li Mu Zhao 《World Journal of Gastroenterology》 SCIE CAS 2015年第9期2854-2857,共4页
Fibrin glue is widely used in clinical practice and plays an important role in reducing postoperative complications.We report a case of a 65-year-old man, whose common bile duct was injured by fibrin glue, with a hist... Fibrin glue is widely used in clinical practice and plays an important role in reducing postoperative complications.We report a case of a 65-year-old man, whose common bile duct was injured by fibrin glue, with a history of failed laparoscopic cholecystectomy and open operation for uncontrolled laparoscopic bleeding.In view of thepersistent liver dysfunction, xanthochromia and skin itching, the patient was admitted to us for further management.Ultrasound, computed tomography, and magnetic resonance cholangiopancreatography(MRCP) revealed multiple stones in the common bile duct, and liver function tests confirmed the presence of obstructive jaundice and liver damage.Endoscopic retrograde cholangiopancreatography was unsuccessfully performed to remove choledocholithiasis, but a small amount of tissue was removed and pathologically confirmed as calcified biliary mucosa.This was followed by open surgery for suspicious cholangiocarcinoma.There was no evidence of cholangiocarcinoma, but the common bile duct wall had a defect of 8 mm × 10 mm at Calot's triangle.A hard, grid-like foreign body was removed, which proved to be solid fibrin glue.Subsequently, the residual choledocholithiasis was removed by a choledochoscopic procedure, and the common bile duct deletion was repaired by liver round ligament with T-tube drainage.Six months later, endoscopy was performed through the T-tube fistula and showed a well-repaired bile duct wall.Eight months later, MRCP confirmed no bile duct stenosis.A review of reported cases showed that fibrin glue is widely used in surgery, but it can also cause organ damage.Its mechanism may be related to discharge reactions. 展开更多
关键词 fibrin GLUE LAPAROSCOPIC CHOLECYSTECTOMY Choledoch
下载PDF
上一页 1 2 114 下一页 到第
使用帮助 返回顶部