亲水性ObeliscR液相色谱-串联质谱法测定猪肉样品中氨基糖苷类药物残留

建立了高效液相色谱-串联质谱法测定猪肉中9种氨基糖苷类药物(壮观霉素、潮霉素B、双氢链霉素、链霉素、阿米卡星、卡那霉素、安普霉素、妥布霉素、庆大霉素)残留量。待测样品中的氨基糖苷类药物经磷酸盐缓冲溶液提取,调节pH值后,经SupelMIP~ SPE-Aminoglycosides分子印迹固相萃取柱净化、浓缩、定容,然后采用Obelisc R高效液相色谱柱分离,以串联质谱法正离子模式检测。该方法在20~1 000μg/L范围内线性关系良好。在线性范围内,分别进行高、中、低3个浓度的添加实验,样品的回收率在76.9%~89.4%之间,相对标准偏差在3.56%~11.4%之间。该方法简便、快速、准确,可以满足猪肉中氨基糖苷类药物残留的测定需求。

人血浆中非那雄胺的HPLC-MS法测定

目的 建立人血浆中非那雄胺的HPLC MS测定法 ,以测定志愿者口服非那雄胺片剂后的血药浓度 ,并对供试制剂和参比制剂的生物等效性进行评价。方法 血样经 0 1mol·L-1 NaOH碱化后用重蒸乙酸乙酯提取 ,进行HPLC MS分析 ,色谱柱为HypersilODS (5μm ,2 50mm× 4 6mm) ,流动相为甲醇 水 (85∶1 5) ,内标为甲地孕酮 ,检测离子为m z 395(非那雄胺 )、m z 40 7(内标 ) ,裂解电压为 1 2 0V。 2 0名健康志愿者交叉口服供试片和参比片 ,计算主要药代动力学参数及相对生物利用度 ,以判断生物等效性。在 1～ 2 0 0 μg·L-1 非那雄胺与内标峰面积比值与浓度线性关系良好 (r=0 9986) ,检测限为 0 0 5μg·L-1 ,回收率为 85 9%～ 98 7%。以AUC0 -2 4计算的非那雄胺片剂的相对生物利用度为 (1 0 0± 1 3) %。结论 建立的分析方法灵敏、准确、简便。统计学结果表明两种制剂生物等效。

Tyrosine promotes anthocyanin biosynthesis in pansy(Viola×wittrockiana)by inducing ABA synthesis

Viola×wittrockiana(pansy)is an important ornamental plant,particularly during winter and spring.In previous studies,we found that the tyrosine decarboxylase gene of pansy(VwTYDC)was expressed differently in blotched and non-blotched areas of pansy petals,suggesting that tyrosine may have a role in anthocyanin biosynthesis.In this study,we found that virus-induced gene silencing of VwTYDC caused an accumulation of pink pigmentation in pansy petals.Likewise,exogenous tyrosine treatment(TYRT)induced the formation of black stripes in nonblotched petal areas.Metabolome analysis indicated that the contents of two anthocyanins,cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside,increased significantly in the TYRT areas.RT-qPCR results revealed that the anthocyanin-related genes VwHCT,VwC3′H,VwCHS,and VwUGT were upregulated in the same areas.Transcriptome analysis revealed that four genes involved in the abscisic acid(ABA)biosynthesis pathway(VwNCED,VwABA2,VwAAO3,and VwCYP707A)were significantly upregulated in the same TYRT areas.ABA content was measured by ESI-HPLCMS/MS,and ABA content was significantly higher in TYRT areas than in control areas.In addition,when exogenous ABA was spread onto nonblotched petal areas,anthocyanin biosynthesis genes were upregulated just as with tyrosine.Thus,transcriptome and metabolite analyses revealed a possible novel regulatory network for anthocyanin biosynthesis in which tyrosine induces ABA synthesis and ABA then promotes anthocyanin biosynthesis in pansy petals.

快速、可靠地定量分析生物毒素 UPLC／MS／MS法分析贝类中亲脂性海洋生物毒素

本文采用UPLC／MS／MS法对海洋中生物毒素进行定量分析。这是一种能够快速、可靠地分析贝类中管制和非管制亲脂性海洋生物毒素的方法。与传统的HPLC／MS／MS方法相比．其分析速度提高了4倍，单次分析时间为5min.可用于代替传统的小鼠生物检测法。