采用2×2实验设计,研究不同家系种质(G♂×S♀、S♂×S♀)和日粮蛋白含量(37%、40%)对黄颡鱼幼鱼生长性能及肝脏IGF-I m RNA表达水平的影响及其交互作用。各试验组设3个平行,养殖60 d。分析结果显示:日粮蛋白含量、家系两...采用2×2实验设计,研究不同家系种质(G♂×S♀、S♂×S♀)和日粮蛋白含量(37%、40%)对黄颡鱼幼鱼生长性能及肝脏IGF-I m RNA表达水平的影响及其交互作用。各试验组设3个平行,养殖60 d。分析结果显示:日粮蛋白含量、家系两因素对末重、增重率、特定生长率及IGF-I m RNA的相对表达量等指标均影响显著(P<0.05),两因素在对上述指标的影响上均不存在交互作用。P40/(G♂×S♀)获得最大末重、增重率和IGF-I基因表达量。结果表明黄颡鱼幼鱼可通过种质筛选和日粮蛋白营养调节达到更好的养殖效果,G♂×S♀家系在高蛋白日粮投喂下,获得最佳生长性能。展开更多
[ Objective] To detect the mRNA of muscle insulin-like growth factorl （IGF-I） in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growt...[ Objective] To detect the mRNA of muscle insulin-like growth factorl （IGF-I） in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [ Method] With real-time quan- titative PCR, the muscle IGF-I mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [ Result] The IGF-I mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-I expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old （ P 〉0.05）, but was lower than that of the latter with extremely significant difference （ P 〈0.01 ） from 30 to 60 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-I mRNA, but the expression level is different between these two species.展开更多
文摘采用2×2实验设计,研究不同家系种质(G♂×S♀、S♂×S♀)和日粮蛋白含量(37%、40%)对黄颡鱼幼鱼生长性能及肝脏IGF-I m RNA表达水平的影响及其交互作用。各试验组设3个平行,养殖60 d。分析结果显示:日粮蛋白含量、家系两因素对末重、增重率、特定生长率及IGF-I m RNA的相对表达量等指标均影响显著(P<0.05),两因素在对上述指标的影响上均不存在交互作用。P40/(G♂×S♀)获得最大末重、增重率和IGF-I基因表达量。结果表明黄颡鱼幼鱼可通过种质筛选和日粮蛋白营养调节达到更好的养殖效果,G♂×S♀家系在高蛋白日粮投喂下,获得最佳生长性能。
文摘[ Objective] To detect the mRNA of muscle insulin-like growth factorl （IGF-I） in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [ Method] With real-time quan- titative PCR, the muscle IGF-I mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [ Result] The IGF-I mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-I expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old （ P 〉0.05）, but was lower than that of the latter with extremely significant difference （ P 〈0.01 ） from 30 to 60 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-I mRNA, but the expression level is different between these two species.
