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化瘀通络灸对血管性痴呆大鼠RhoA/ROCK轴突生长抑制性信号通路相关蛋白表达的影响
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作者 邱市生 王珊 唐友斌 《中医研究》 2025年第1期72-76,共5页
目的:观察化瘀通络灸对血管性痴呆大鼠Ras同源物基因组成员A(Ras homolog gene family member A,RhoA)/Rho激酶(Rho associated protein kinase, ROCK)轴突生长抑制性信号通路相关蛋白的影响,探讨其治疗血管性痴呆的机制。方法:使用Mor... 目的:观察化瘀通络灸对血管性痴呆大鼠Ras同源物基因组成员A(Ras homolog gene family member A,RhoA)/Rho激酶(Rho associated protein kinase, ROCK)轴突生长抑制性信号通路相关蛋白的影响,探讨其治疗血管性痴呆的机制。方法:使用Morris水迷宫筛除贴壁、原地旋转或溺水的Wistar大鼠,将符合条件大鼠分为假手术组、造模组。假手术组暴露大鼠颈总动脉;造模组用改良的双侧颈总动脉永久结扎术制备血管性痴呆大鼠模型,术后3 d用水迷宫对大鼠进行模型鉴定,将合格者随机分为模型组、艾灸组和西药组。于鉴定次日对大鼠进行治疗,艾灸组悬灸百会、大椎、神庭,20 min/次,1次/d, 1周为1疗程,2个疗程间休息1 d,共3个疗程;西药组用吡拉西坦溶液灌胃,2次/d;假手术组、模型组行灸架固定。治疗结束,取大鼠脑组织,用免疫荧光单染法、蛋白免疫印迹法检测其脑内目的蛋白RhoA、Rho相关螺旋卷曲蛋白激酶Ⅱ(Rho protein-related curl spiral kinase-Ⅱ,ROCKⅡ)、肌球蛋白轻链磷酸化(Phosphorylation of myosin light chain, P-MLC)的阳性表达。结果:模型组大鼠海马、皮质RhoA、ROCKⅡ、P-MLC蛋白表达高于假手术组(P<0.01);艾灸组、西药组海马和皮质RhoA、ROCKⅡ、P-MLC蛋白表达较模型组低(P<0.05);艾灸组皮质ROCKⅡ蛋白表达较西药组稍低(P<0.05);艾灸组与西药组海马ROCKⅡ、海马与皮质RhoA、P-MLC蛋白表达差异无统计学意义(P>0.05)。结论:化瘀通络灸可能是通过下调RhoA/ROCK轴突生长抑制性信号通路相关蛋白的表达提高血管性痴呆大鼠的记忆力,从而发挥治疗血管性痴呆的作用。 展开更多
关键词 血管性痴呆 化瘀通络灸 动物模型 大鼠 RhoA/rock信号通路 海马 皮质
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Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements
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作者 Peng Li Meifeng Cai +2 位作者 Shengjun Miao Yuan Li Yu Wang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS 2025年第1期39-48,共10页
Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress magnitude.To investigate the correlation between the rock mass properties and maximum horizontal stre... Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress magnitude.To investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution method.The results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with depth.There is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and messy.Moreover,there is no obvious relationship between the rock quality designation(RQD)and depth.The maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same depth.In addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data. 展开更多
关键词 overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification
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参附注射液通过NgR1/Lingo-1/p75NTR复合物及RhoA/ROCKⅡ信号通路减轻心肺复苏脑损伤机制的研究
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作者 肖佳佳 邓海霞 吴鑫宇 《中国中医急症》 2025年第1期33-36,50,共5页
目的观察参附注射液对心脏骤停后脑缺血再灌注损伤大鼠的神经保护作用,研究其是否通过调控NgR1/RhoA/ROCKⅡ信号通路及其上游因子Nogo受体相互作用蛋白1(Lingo-1)和p75神经营养因子受体(p75NTR),减轻脑损伤并促进神经轴突再生。方法制... 目的观察参附注射液对心脏骤停后脑缺血再灌注损伤大鼠的神经保护作用,研究其是否通过调控NgR1/RhoA/ROCKⅡ信号通路及其上游因子Nogo受体相互作用蛋白1(Lingo-1)和p75神经营养因子受体(p75NTR),减轻脑损伤并促进神经轴突再生。方法制备心脏骤停/心肺复苏大鼠模型,将大鼠分为模型组、参附组、拮抗剂组及参拮组。通过ELISA检测各组脑组织中NgR1、p75NTR、Lingo-1、RhoA和RockⅡ的表达水平,并通过HE染色观察脑组织损伤情况。结果ELISA结果显示,心脏骤停后大鼠脑皮层中,模型组的各指标表达在特定时间点显著升高,尤其在3 d时NgR1、p75NTR、RhoA和ROCKⅡ达到峰值,显著高于空白组(P<0.01);参附组、拮抗剂组及参拮组的各指标表达水平均显著低于模型组(P<0.01),其中参拮组在所有时间点均表现出较低的NgR1、p75NTR、Lingo-1、RhoA及ROCKⅡ水平,尤其在3 d和7 d的ROCKⅡ和RhoA表达方面,显著低于模型组(P<0.01),RhoA在7 d时显著低于空白组(P<0.01)。HE染色结果表明,参附组和参拮组在各时间点的脑组织结构恢复效果优于模型组,细胞排列改善,水肿减轻。结论参附注射液通过调控NgR1/RhoA/ROCKⅡ信号通路及其上游因子,减轻了心脏骤停后脑缺血再灌注损伤,促进了神经轴突再生与功能恢复。 展开更多
关键词 心肺复苏 脑损伤 参附注射液 NgR1/p75NTR/Lingo-1复合物 RhoA/rockⅡ信号通路 神经轴突再生
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Aluminum foam as buffer layer used in soft rock tunnel with large deformation
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作者 WU Faquan MIAO Binxin +2 位作者 TIAN Yun ZHANG Fang ZHANG Chaoxuan 《Journal of Mountain Science》 2025年第1期324-336,共13页
The squeezing deformation of surrounding rock is an important factor restricting the safe construction and long-term operation of tunnels when a tunnel passes through soft strata with high ground stress.Under such sof... The squeezing deformation of surrounding rock is an important factor restricting the safe construction and long-term operation of tunnels when a tunnel passes through soft strata with high ground stress.Under such soft rock geological conditions,the large deformation of the surrounding rock can easily lead to the failure of supporting structures,including shotcrete cracks,spalling,and steel arch distortion.To improve the lining support performance during the large deformation of squeezed surrounding rock,this work selects aluminum foam with densities of 0.25 g/cm3,0.42 g/cm3 and 0.61 g/cm3 as the buffer layer material and carries out uniaxial confined compression tests.Through the evaluation and analysis of energy absorption and the comparison of the yield pressure of aluminum foam with those of other cushioning materials and yield pressure support systems,the strength,deformation and energy absorption of aluminum foam with a density of 0.25 g/cm3 meet the yield pressure performance requirements.The numerical model of the buffer layer yielding support system is then established via the finite element analysis software ABAQUS,and the influence of the buffer layer setting on the lining support is analyzed.Compared with the conventional support scheme,the addition of an aluminum foam buffer layer can reduce the stress and deformation of the primary support and secondary lining.The maximum and minimum principal stresses of the primary support are reduced by 13%and 15%,respectively.The maximum and minimum principal stresses of the secondary lining are reduced by 15%and 12%,respectively,and the displacement deformation of the secondary lining position is reduced by 15%.In summary,the application of aluminum foam buffer layer can reduce the stress and deformation of the primary support and secondary lining,improve the stress safety of the support and reduce the deformation of the support. 展开更多
关键词 Soft rock tunnel Uniaxial confined compression Aluminum foam Buffer layer Yielding support
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Effect of surface crack on the bearing capacity of strip footing placed on rock mass
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作者 Avneet LAHARIYA Debarghya CHAKRABORTY 《Journal of Mountain Science》 2025年第1期337-353,共17页
In hilly regions,the existence of surface cracks in rock mass induces a potential threat to structural stability.Thus,the present research aims to explore the impact of surface cracks on the loadbearing capacity of st... In hilly regions,the existence of surface cracks in rock mass induces a potential threat to structural stability.Thus,the present research aims to explore the impact of surface cracks on the loadbearing capacity of strip footing placed on the rock mass.By taking into account the various boundary constraints across the surface of crack edges,the study investigates the presence of two categories of surface cracks,namely(1)separated crack,and(2)fine crack.The lower bound limit analysis is employed in conjunction with the finite element method(LBFELA)to conduct the numerical analysis.In order to evaluate rock mass yielding,the power conic programming(PCP)method is utilized to implement the generalized Hoek-Brown(GHB)failure criterion.The stability of the strip footing is analyzed by determining the bearing capacity factor(Nσγ),which is presented in the form of design charts by varying the strength parameters of rock,including the Geological Strength Index(GSI),Hoek-Brown material parameter(mi),Disturbance factor(D),and Normalised Uniaxial Compressive Strength(σci/γB),whereγis the unit weight of rock mass,and B is the width of strip footing.The study also investigates the impact of cracks on strip footings,considering different positions of the crack(LC)and depths of the crack(DC).The results demonstrate that the influence of the fine crack is only noticeable until the LC/B ratio reaches 6.However,for the separated crack,its impact remains significant even when the LC/B ratio exceeds 16.The appearance of fine crack at the edge of the footing results in a decrease in the magnitude Nσγof up to 45%,indicating a substantial reduction in the stability of the footing.The failure patterns are presented and discussed in detail for various cases in this study to examine the effect of surface cracks on the strip footing and to address the extent of the plastic collapse. 展开更多
关键词 rock mass Surface cracks Strip footing Power cone programming GHB failure criterion Limit analysis
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Disturbance failure mechanism of highly stressed rock in deep excavation:Current status and prospects 被引量:3
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作者 Tao Wang Weiwei Ye +3 位作者 Liyuan Liu Kai Liu Naisheng Jiang Xianhui Feng 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第4期611-627,共17页
This article reviews the current status on the dynamic behavior of highly stressed rocks under disturbances.Firstly,the experimental apparatus,methods,and theories related to the disturbance dynamics of deep,high-stre... This article reviews the current status on the dynamic behavior of highly stressed rocks under disturbances.Firstly,the experimental apparatus,methods,and theories related to the disturbance dynamics of deep,high-stress rock are reviewed,followed by the introduction of scholars’research on deep rock deformation and failure from an energy perspective.Subsequently,with a backdrop of highstress phenomena in deep hard rock,such as rock bursts and core disking,we delve into the current state of research on rock microstructure analysis and residual stresses from the perspective of studying the energy storage mechanisms in rocks.Thereafter,the current state of research on the mechanical response and the energy dissipation of highly stressed rock formations is briefly retrospected.Finally,the insufficient aspects in the current research on the disturbance and failure mechanisms in deep,highly stressed rock formations are summarized,and prospects for future research are provided.This work provides new avenues for the research on the mechanical response and damage-fracture mechanisms of rocks under high-stress conditions. 展开更多
关键词 deep rock with high stress highly stressed rock rock failure residual stress energy release
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Rho/ROCK信号通路在糖尿病视网膜病变中的研究进展
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作者 唐云华 刘春梦 +2 位作者 邢尧 柯殷雨 张富文 《中医眼耳鼻喉杂志》 2024年第3期152-155,共4页
糖尿病视网膜病变(diabetic retinopathy,DR)是导致劳动年龄人群视力障碍的重要原因,其复杂的发病机制尚未完全明确。虽然一些新兴治疗方法在DR的治疗中取得显著疗效,但仍存在治疗效果欠佳及并发症等问题。Rho GTP酶是一种小分子蛋白,... 糖尿病视网膜病变(diabetic retinopathy,DR)是导致劳动年龄人群视力障碍的重要原因,其复杂的发病机制尚未完全明确。虽然一些新兴治疗方法在DR的治疗中取得显著疗效,但仍存在治疗效果欠佳及并发症等问题。Rho GTP酶是一种小分子蛋白,通过激活下游蛋白ROCK参与细胞骨架调节等多种细胞活动。在DR发病过程中Rho/ROCK信号通路被激活,抑制Rho/ROCK信号传导可能抑制DR的进展。本文从炎症、新生血管、血-视网膜屏障(blood retinal barrier,BRB)、晚期糖基化终末产物(advanced glycation end products,AGEs)、糖尿病视网膜神经变性(diabetic retinal neurodegeneration,DRN)等方面阐述Rho/ROCK信号通路在DR发病机制中的研究进展,以期为DR的治疗提供新思路。 展开更多
关键词 糖尿病视网膜病变 Rho GTP酶 rock RHO/rock信号通路 rock抑制剂
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Coupled DEM-FDM analyses of the effects of falling rock’s shape and impact angle on response of granular cushion and rock shed
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作者 Jingyu Xue Chen Cao +2 位作者 Jianhua Yan Yaopeng Ji Jianping Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第8期3353-3364,共12页
Rock shed is an effective protection measure against rockfall.To investigate the influences of falling rock’s shape and impact angle on the impact effect of the cushioned rock shed,a modeling approach for a rock shed... Rock shed is an effective protection measure against rockfall.To investigate the influences of falling rock’s shape and impact angle on the impact effect of the cushioned rock shed,a modeling approach for a rock shed with a cushion layer using PFC-FLAC.The granular cushion is modeled as an aggregate of discrete non-cohesion particles,while the concrete plate and the beam are modeled as zones.The falling rock with different sphericities and impact angles is modeled as a rigid assembly.The numerical model is validated by comparing the simulation results with experimental and numerical results from previous literature.This model is applied to analyze the effects of rock shape and impact angle on the dynamic interaction effects between falling rock and cushioned rock shed,including the impact force,transmitted bottom force,penetration depth,and plate deflection.The numerical results show that the variation in the falling rock’s shape has different effects on the falling rock with different impact angles.These findings could support rock shed design by revealing the limitations of the assumptions in the past research,which may result in unsafe rock sheds for some rockfall cases. 展开更多
关键词 rockfall Coupled DEM-FDM analysis rock’s shape Impact angle Granular cushion rock shed
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Numerical modeling of blast-induced rock fragmentation in deep mining with 3D and 2D FEM method approaches
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作者 Michał Kucewicz Mazurkiewicz Łukasz +4 位作者 Paweł Baranowski Jerzy Małachowski Krzysztof Fuławka Piotr Mertuszka Marcin Szumny 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第11期4532-4553,共22页
To optimize the excavation of rock using underground blasting techniques,a reliable and simplified approach for modeling rock fragmentation is desired.This paper presents a multistep experimentalnumerical methodology ... To optimize the excavation of rock using underground blasting techniques,a reliable and simplified approach for modeling rock fragmentation is desired.This paper presents a multistep experimentalnumerical methodology for simplifying the three-dimensional(3D)to two-dimensional(2D)quasiplane-strain problem and reducing computational costs by more than 100-fold.First,in situ tests were conducted involving single-hole and free-face blasting of a dolomite rock mass in a 1050-m-deep mine.The results were validated by laser scanning.The craters were then compared with four analytical models to calculate the radius of the crushing zone.Next,a full 3D model for single-hole blasting was prepared and validated by simulating the crack length and the radius of the crushing zone.Based on the stable crack propagation zones observed in the 3D model and experiments,a 2D model was prepared.The properties of the high explosive(HE)were slightly reduced to match the shape and number of radial cracks and crushing zone radius between the 3D and 2D models.The final methodology was used to reproduce various cut-hole blasting scenarios and observe the effects of residual cracks in the rock mass on further fragmentation.The presence of preexisting cracks was found to be crucial for fragmentation,particularly when the borehole was situated near a free rock face.Finally,an optimization study was performed to determine the possibility of losing rock continuity at different positions within the well in relation to the free rock face. 展开更多
关键词 Dolomite rock Two-dimensional(2D)rock modeling rock fragmentation Cut-hole blasting
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Field observations and interpretation of extensional fracture in hard rock surrounding deep underground openings
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作者 Charlie C.Li 《Deep Resources Engineering》 2024年第1期1-12,共12页
Extensional fracturing often occurs in hard rock masses during excavation at depths,for example,>1000 m below the ground surface.Surface-parallel fractures are created in the surrounding rock mass,which is typicall... Extensional fracturing often occurs in hard rock masses during excavation at depths,for example,>1000 m below the ground surface.Surface-parallel fractures are created in the surrounding rock mass,which is typically subjected to stresses parallel to the free rock surfaces after excavation.These are called extensional fractures because the strains perpendicular to the fracture planes are extensional and the opposite surfaces of each fracture tend to separate from each other as soon as the fracture is created.These fractures predominantly propagate parallel to the maximum principal stressσ1 in the surrounding rock mass.This study analyses extensional fractures observed during excavations in cut-and-fill mining stopes in a deep metal mine.This analysis explores the process of extensional fracturing during excavation in an undisturbed rock mass.In general,intensive spalling occurred on the roof surfaces immediately after the excavation of the undisturbed rock mass.This spalling terminated after a certain depth of rock failure,while burst sounds intermediately emitted from the surrounding rock mass,indicating that rock fracturing was ongoing at depth.In the subsequent cutting slices,the spacing between the extensional fractures decreased with increasing mine-out space in the stope.An extensional fracturing criterion was proposed based on microscopic observations of microcrack development in the rock in response to applied stress.The crack initiation and extensional fracturing processes are associated with two critical extensional strains which are related to the secondary stress state in the position.In areas close to the free rock surface whereσ3=0,the stress for crack initiation is(σ1+σ2)=0.4σc,whereas the stress for extensional fracturing is(σ1+σ2)=0.8σc. 展开更多
关键词 Extensional fracture rock fracture Failure criterion rock burst Hard rock
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Rockburst risk control and mitigation in deep mining
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作者 Ming Cai 《Deep Resources Engineering》 2024年第2期46-67,共22页
It is important to understand and manage rockburst challenges in deep mining operations.This paper presents a systematic study of rockburst risk in underground mining,offering a detailed examination of influencing fac... It is important to understand and manage rockburst challenges in deep mining operations.This paper presents a systematic study of rockburst risk in underground mining,offering a detailed examination of influencing factors,risk assessment,and various control and mitigation methods.The complexities of rockburst phenomena are explained by examining factors that lead to the occurrence of rockbursts.A rockburst risk assessment using a bow-tie analysis is conducted,which provides insights into both risk evaluation and proactive control and mitigation systems.The core of the paper presents a comprehensive array of rockburst risk control and mitigation methods,which range from controls to reduce rockburst hazard,and excavation vulnerability,to controls and mitigations to reduce exposure.Strategic engineering control methods,including mine design and mining sequencing,are discussed.Tactical engineering control measures,such as ground pre-conditioning and rock support,are scrutinized,along with administrative controls like evacuation and re-entry protocols and the use of mechanized equipment.A multiple-line defense system is advocated for rockburst risk management to address the uncertainties involved in the process.Finally,emerging technologies and innovations as well as challenges are discussed,providing a roadmap for continued advancements in rockburst risk management in the future.This work serves as a valuable resource for mining professionals,researchers,and policymakers seeking a comprehensive understanding of rockburst risk management in deep mining. 展开更多
关键词 rockburst risk rockburst hazard Excavation vulnerability Exposure rock support Risk control Risk mitigation
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SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能 被引量:1
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作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p rock1
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Impact resistance performance and optimization of the sand-EPE composite cushion in rock sheds 被引量:2
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作者 YU Bingxin ZHOU Xiaojun +2 位作者 TANG Jianhui ZHANG Yujin ZHANG Yuefeng 《Journal of Mountain Science》 SCIE CSCD 2024年第2期676-689,共14页
Rock sheds are widely used to prevent rockfall disasters along roads in mountainous areas.To improve the capacity of rock sheds for resisting rockfall impact,a sand and expandable polyethylene(EPE)composite cushion wa... Rock sheds are widely used to prevent rockfall disasters along roads in mountainous areas.To improve the capacity of rock sheds for resisting rockfall impact,a sand and expandable polyethylene(EPE)composite cushion was proposed.A series of model experiments of rockfall impact on rock sheds were conducted,and the buried depth of the EPE foam board in the sand layer was considered.The impact load and dynamic response of the rock shed were investigated.The results show that the maximum impact load and dynamic response of the rock shed roof are all significantly less than those of the sand cushion.Moreover,as the distance between the EPE foam board and rock shed roof decreases,the maximum rockfall impact force and impact pressure gradually decrease,and the maximum displacement,acceleration and strain of the rock shed first decrease and then change little.In addition,the vibration acceleration and vertical displacement of the rock shed roof decrease from the centre to the edge and decrease faster along the longitudinal direction than that along the transverse direction.In conclusion,the buffering effect of the sand-EPE composite cushion is better than that of the pure sand cushion,and the EPE foam board at a depth of 1/3 the thickness of the sand layer is appropriate. 展开更多
关键词 rockFALL rock shed Impact Composite cushion Buffering effect Dynamic response
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A statistical damage-based constitutive model for shearing of rock joints in brittle drop mode 被引量:1
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作者 Xinrong Liu Peiyao Li +5 位作者 Xueyan Guo Xinyang Luo Xiaohan Zhou Luli Miao Fuchuan Zhou Hao Wang 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第8期1041-1058,共18页
Some rock joints exhibit significant brittleness,characterized by a sharp decrease in shear stress upon reaching the peak strength.However,existing models often fail to accurately represent this behavior and are encum... Some rock joints exhibit significant brittleness,characterized by a sharp decrease in shear stress upon reaching the peak strength.However,existing models often fail to accurately represent this behavior and are encumbered by numerous parameters lacking clear mechanical significance.This study presents a new statistical damage constitutive model rooted in both damage mechanics and statistics,containing only three model parameters.The proposed model encompasses all stages of joint shearing,including the compaction stage,linear stage,plastic yielding stage,drop stage,strain softening stage,and residual strength stage.To derive the analytical expression of the constitutive model,three boundary conditions are introduced.Experimental data from both natural and artificial rock joints is utilized to validate the model,resulting in average absolute relative errors ranging from 3%to 8%.Moreover,a comparative analysis with established models illustrates that the proposed model captures stress drop and post-peak strain softening more effectively,with model parameters possessing clearer mechanical interpretations.Furthermore,parameter analysis is conducted to investigate the impacts of model parameters on the curves and unveil the relationship between these parameters and the mechanical properties of rock joints.Importantly,the proposed model is straightforward in form,and all model parameters can be obtained from direct shear tests,thus facilitating the utilization in numerical simulations. 展开更多
关键词 rock joints Brittle rock Direct shear test Damage-based constitutive model Parameters analysis
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Predicting the probability distribution of Martian rocks mechanical property based on microscale rock mechanical experiments and accurate grain-based modeling 被引量:1
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作者 Shuohui Yin Yingjie Wang Jingang Liu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第9期1327-1339,共13页
The exploration of Mars would heavily rely on Martian rocks mechanics and engineering technology.As the mechanical property of Martian rocks is uncertain,it is of utmost importance to predict the probability distribut... The exploration of Mars would heavily rely on Martian rocks mechanics and engineering technology.As the mechanical property of Martian rocks is uncertain,it is of utmost importance to predict the probability distribution of Martian rocks mechanical property for the success of Mars exploration.In this paper,a fast and accurate probability distribution method for predicting the macroscale elastic modulus of Martian rocks was proposed by integrating the microscale rock mechanical experiments(micro-RME),accurate grain-based modeling(AGBM)and upscaling methods based on reliability principles.Firstly,the microstructure of NWA12564 Martian sample and elastic modulus of each mineral were obtained by micro-RME with TESCAN integrated mineral analyzer(TIMA)and nanoindentation.The best probability distribution function of the minerals was determined by Kolmogorov-Smirnov(K-S)test.Secondly,based on best distribution function of each mineral,the Monte Carlo simulations(MCS)and upscaling methods were implemented to obtain the probability distribution of upscaled elastic modulus.Thirdly,the correlation between the upscaled elastic modulus and macroscale elastic modulus obtained by AGBM was established.The accurate probability distribution of the macroscale elastic modulus was obtained by this correlation relationship.The proposed method can predict the probability distribution of Martian rocks mechanical property with any size and shape samples. 展开更多
关键词 Probability distribution Martian rocks Microscale rock mechanic experiment Nanoindentation Accurate grain-based modeling
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贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响
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作者 彭博文 冯立 覃剑 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第7期980-984,共5页
目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组... 目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组、贝母素乙(3.5 mg/kg)+LPA(RhoA激活剂,1 mg/kg)组,每组10只,另选10只大鼠为对照组。机械刺激法和热辐射法检测大鼠膝关节疼痛症状;检测大鼠关节肿胀度、膝关节宽度并以步态评分评测其关节功能;透射电镜检测大鼠膝关节软骨细胞超微结构;TUNEL染色检测大鼠关节软骨细胞凋亡比;酶联免疫吸附(ELISA)法检测大鼠血清和关节液促炎因子白细胞介素(IL)-18、单核细胞趋化蛋白1(MCP-1)水平;免疫印迹检测各组大鼠关节软骨组织凋亡(Cleaved caspase-3、Bax)和RhoA/ROCK通路相关蛋白表达。结果 贝母素乙可降低KOA大鼠关节肿胀度、膝关节宽度、步态评分、软骨细胞凋亡比、血清及关节液IL-18、MCP-1水平、软骨组织Cleaved caspase-3、Bax、RhoA、ROCK1、ROCK1蛋白表达,升高机械痛阈值、热痛阈值(P<0.05);LPA可减弱贝母素乙对KOA大鼠关节软骨损伤的改善作用。结论 贝母素乙可通过抑制RhoA/ROCK信号激活而抑制KOA大鼠关节炎症反应和关节软骨细胞凋亡,减轻软骨损伤。 展开更多
关键词 贝母素乙 RhoA/rock 膝骨关节炎 软骨损伤 信号通路
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益母草碱调节RhoA/ROCK信号通路对乙型肝炎大鼠的治疗作用
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作者 王维 穆宝龙 +3 位作者 张文双 吴清雷 张慧慧 曹智丽 《广州中医药大学学报》 CAS 2024年第12期3259-3265,共7页
【目的】观察益母草碱对乙型肝炎大鼠的治疗作用及机制。【方法】将60只大鼠随机分为6组,即正常组,模型组,益母草碱低、中、高剂量组,益母草碱高剂量+溶血磷脂酸(LPA)组。除正常组外,其余各组大鼠构建乙型肝炎病毒(HBV)感染模型。造模... 【目的】观察益母草碱对乙型肝炎大鼠的治疗作用及机制。【方法】将60只大鼠随机分为6组,即正常组,模型组,益母草碱低、中、高剂量组,益母草碱高剂量+溶血磷脂酸(LPA)组。除正常组外,其余各组大鼠构建乙型肝炎病毒(HBV)感染模型。造模成功后,各组对应干预。干预结束后,采用酶联免疫吸附分析(ELISA)检测血清乙型肝炎e抗原(HBeAg)、乙型肝炎表面抗原(HBsAg)及炎症因子白细胞介素(IL)-10、IL-6、肿瘤坏死因子α(TNF-α)水平,自动分析仪测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平,实时定量聚合酶链反应(RT-qPCR)法检测肝组织HBV DNA水平,苏木素-伊红(HE)染色法观察肝组织病理变化,免疫组织化学法检测肝组织乙型肝炎核心抗原(HBcAg)表达水平,Western Blot法检测肝组织Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达水平。【结果】与正常组比较,模型组大鼠肝小叶中心坏死,肝细胞水肿、变性,HBsAg、HBeAg、HBV DNA水平,HBcAg阳性细胞数,ALT、AST水平,IL-6、TNF-α、IL-10含量,RhoA、ROCK1、ROCK2表达水平均显著升高(均P<0.05);与模型组比较,益母草碱低、中、高剂量组大鼠肝损伤程度降低,肝细胞变性减轻,HBsAg、HBeAg、HBV DNA水平,HBcAg阳性细胞数,ALT、AST水平,IL-6、TNF-α含量,RhoA、ROCK1、ROCK2表达水平均显著降低,IL-10含量升高,差异均有统计学意义(P<0.05);与益母草碱高剂量组比较,益母草碱高剂量+LPA组肝损伤程度加重,HBsAg、HBeAg、HBV DNA水平,HBcAg阳性细胞数,ALT、AST水平,IL-6、TNF-α含量,RhoA、ROCK1、ROCK2表达水平升高,IL-10含量降低,差异均有统计学意义(P<0.05)。【结论】益母草碱能减轻乙型肝炎大鼠肝组织损伤,改善肝功能,减少炎症反应,其机制与抑制RhoA/ROCK通路有关。 展开更多
关键词 益母草碱 乙型肝炎 肝功能 炎症反应 RhoA/rock通路 大鼠
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桃叶珊瑚苷调节RhoA/ROCK信号通路对胶质母细胞瘤细胞活力和上皮间质转化的影响
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作者 李娟 石海平 李维民 《天津中医药》 CAS 2024年第3期386-392,共7页
[目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(C... [目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(CCK-8)法检测细胞活力,流式细胞术检测细胞凋亡,Transwell小室实验检测细胞迁移和侵袭,蛋白免疫印迹法(Western Blot)检测基质金属蛋白酶(MMP)2、MMP9、波形蛋白(Vimentin)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。构建GBM裸鼠模型,随机分为裸鼠对照组、AU组、Y-27632组、AU+Y-27632组,测量肿瘤质量与体积,免疫组化法检测移植瘤组织RhoA、ROCK1、ROCK2蛋白表达。[结果]GBM细胞活力随着AU浓度的升高而逐渐降低(P<0.05),选择U87作为后续实验细胞,选择10、25、50μmol/L浓度作为AU后续实验浓度。与对照组比较,AU低、中、高浓度组和Y-27632组细胞活力、迁移和侵袭细胞数、MMP2、MMP9、N-cadherin、Vimentin、RhoA、ROCK1、ROCK2表达显著下降,凋亡率、E-cadherin表达显著升高(P<0.05),其中高浓度AU和Y-27632组共同处理的细胞变化更显著(P<0.05)。AU和Y-27632均能抑制移植瘤质量和体积,降低RhoA/ROCK信号通路蛋白表达(P<0.05)。[结论]AU能抑制GBM细胞活力、迁移侵袭和EMT,促进细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 胶质母细胞瘤 细胞活力 上皮间质转化 桃叶珊瑚苷 RhoA/rock信号通路
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Discontinuity development patterns and the challenges for 3D discrete fracture network modeling on complicated exposed rock surfaces 被引量:1
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作者 Wen Zhang Ming Wei +8 位作者 Ying Zhang Tengyue Li Qing Wang Chen Cao Chun Zhu Zhengwei Li Zhenbang Nie Shuonan Wang Han Yin 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2154-2171,共18页
Natural slopes usually display complicated exposed rock surfaces that are characterized by complex and substantial terrain undulation and ubiquitous undesirable phenomena such as vegetation cover and rockfalls.This st... Natural slopes usually display complicated exposed rock surfaces that are characterized by complex and substantial terrain undulation and ubiquitous undesirable phenomena such as vegetation cover and rockfalls.This study presents a systematic outcrop research of fracture pattern variations in a complicated rock slope,and the qualitative and quantitative study of the complex phenomena impact on threedimensional(3D)discrete fracture network(DFN)modeling.As the studies of the outcrop fracture pattern have been so far focused on local variations,thus,we put forward a statistical analysis of global variations.The entire outcrop is partitioned into several subzones,and the subzone-scale variability of fracture geometric properties is analyzed(including the orientation,the density,and the trace length).The results reveal significant variations in fracture characteristics(such as the concentrative degree,the average orientation,the density,and the trace length)among different subzones.Moreover,the density of fracture sets,which is approximately parallel to the slope surface,exhibits a notably higher value compared to other fracture sets across all subzones.To improve the accuracy of the DFN modeling,the effects of three common phenomena resulting from vegetation and rockfalls are qualitatively analyzed and the corresponding quantitative data processing solutions are proposed.Subsequently,the 3D fracture geometric parameters are determined for different areas of the high-steep rock slope in terms of the subzone dimensions.The results show significant variations in the same set of 3D fracture parameters across different regions with density differing by up to tenfold and mean trace length exhibiting differences of 3e4 times.The study results present precise geological structural information,improve modeling accuracy,and provide practical solutions for addressing complex outcrop issues. 展开更多
关键词 Complicated exposed rock surfaces Discontinuity characteristic variation Three-dimensional discrete fracture network modeling Outcrop study Vegetation cover and rockfalls
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Rockburst proneness criteria for rock materials:Review and new insights 被引量:52
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作者 GONG Feng-qiang WANG Yun-liang LUO Song 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第10期2793-2821,共29页
To review the rockburst proneness(or tendency)criteria of rock materials and compare the judgment accuracy of them,twenty criteria were summarized,and their judgment accuracy was evaluated and compared based on the la... To review the rockburst proneness(or tendency)criteria of rock materials and compare the judgment accuracy of them,twenty criteria were summarized,and their judgment accuracy was evaluated and compared based on the laboratory tests on fourteen types of rocks.This study begins firstly by introducing the twenty rockburst proneness criteria,and their origins,definitions,calculation methods and grading standards were summarized in detail.Subsequently,to evaluate and compare the judgment accuracy of the twenty criteria,a series of laboratory tests were carried out on fourteen types of rocks,and the rockburst proneness judgment results of the twenty criteria for the fourteen types of rocks were obtained accordingly.Moreover,to provide a unified basis for the judgment accuracy evaluation of above criteria,a classification standard(obtained according to the actual failure results and phenomena of rock specimen)of rockburst proneness in laboratory tests was introduced.The judgment results of the twenty criteria were compared with the judgment results of this classification standard.The results show that the judgment results of the criterion based on residual elastic energy(REE)index are completely consistent with the actual rockburst proneness,and the other criteria have some inconsistent situations more or less.Moreover,the REE index is based on the linear energy storage law and defined in form of a difference value and considered the whole failure process,and these superior characteristics ensure its accuracy.It is believed that the criterion based on REE index is comparatively more accurate and scientific than other criteria,and it can be recommended to be applied to judge the rockburst proneness of rock materials. 展开更多
关键词 deep rock rockBURST rockburst proneness rockburst proneness criterion rock mechanics
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