Control of dust in underground coal mines is critical for mitigating both safety and health hazards.For decades,the National Institute of Occupational Safety and Health(NIOSH)has led research to evaluate the effective...Control of dust in underground coal mines is critical for mitigating both safety and health hazards.For decades,the National Institute of Occupational Safety and Health(NIOSH)has led research to evaluate the effectiveness of various dust control technologies in coal mines.Recent studies have included the evaluation of auxiliary scrubbers to reduce respirable dust downstream of active mining and the use of canopy air curtains(CACs)to reduce respirable dust in key operator positions.While detailed dust characterization was not a focus of such studies,this is a growing area of interest.Using preserved filter samples from three previous NIOSH studies,the current work aims to explore the effect of two different scrubbers(one wet and one dry)and a roof bolter CAC on respirable dust composition and particle size distribution.For this,the preserved filter samples were analyzed by thermogravimetric analysis and/or scanning electron microscopy with energy dispersive X-ray.Results indicate that dust composition was not appreciably affected by either scrubber or the CAC.However,the wet scrubber and CAC appeared to decrease the overall particle size distribution.Such an effect of the dry scrubber was not consistently observed,but this is probably related to the particular sampling location downstream of the scrubber which allowed for significant mixing of the scrubber exhaust and other return air.Aside from the insights gained with respect to the three specific dust control case studies revisited here,this work demonstrates the value of preserved dust samples for follow-up investigation more broadly.展开更多
Exposure to respirable coal mine dust(RCMD)can cause chronic and debilitating lung diseases.Real-time monitoring capabilities are sought which can enable a better understanding of dust components and sources.In many u...Exposure to respirable coal mine dust(RCMD)can cause chronic and debilitating lung diseases.Real-time monitoring capabilities are sought which can enable a better understanding of dust components and sources.In many underground mines,RCMD includes three primary components which can be loosely associated with three major dust sources:coal dust from the coal seam itself,silicates from the surrounding rock strata,and carbonates from the inert‘rock dust’products that are applied to mitigate explosion hazards.A monitor which can reliably partition RCMD between these three components could thus allow source apportionment.And tracking silicates,specifically,could be valuable since the most serious health risks are typically associated with this component-particularly if abundant in crystalline silica.Envisioning a monitoring concept based on field microscopy,and following up on prior research using polarized light,the aim of the current study was to build and test a model to classify respirable-sized particles as either coal,silicates,or carbonates.For model development,composite dust samples were generated in the laboratory by successively depositing dust from high-purity materials onto a sticky transparent substrate,and imaging after each deposition event such that the identity of each particle was known a priori.Model testing followed a similar approach,except that real geologic materials were used as the source for each dust component.Results showed that the model had an overall accuracy of 86.5%,indicating that a field-microscopy based moni-tor could support RCMD source apportionment and silicates tracking in some coal mines.展开更多
Cumulative inhalation of respirable coal mine dust(RCMD)can lead to severe lung diseases,including coal worker's pneu-moconiosis(CWP),silicosis,mixed dust pneumoconiosis,dust-related diffuse fibrosis(DDF),and prog...Cumulative inhalation of respirable coal mine dust(RCMD)can lead to severe lung diseases,including coal worker's pneu-moconiosis(CWP),silicosis,mixed dust pneumoconiosis,dust-related diffuse fibrosis(DDF),and progressive massive fibrosis(PMF).Statistics from the number of reported cases showed a significant decrease in the progression of respiratory diseases in the 1990s.However,an unexpected increase in the number of CWP cases was reported in the late 1990s.To date,there has been no comprehensive systematic review to assess all contributing factors to the resurgence of CWP cases.This study aims to investigate the effects of various mining parameters on the prevalence of CWP in coal mines.A systematic review using the preferred reporting items for systematic reviews and meta-analysis(PRISMA)method was conducted to investigate the health effects of RCMD exposure and identify the factors that may contribute to the recent resurgence of CWP cases.The systematic review yielded a total of 401 papers,which were added to the database.The total number of 148 and 208 papers were excluded from the database in the process of screening and eligibility,respectively.Then,18 papers were considered for data selection and full-text assessment.The review revealed that factors including geographic location,mine size,mining operation type,coal-seam thickness,coal rank,changes in mining practices,technology advancement,and engi-neering dust control practices are contributing to the recent resurgence of CWP among coal workers.However,the evidence for root causes is limited owing to the methodological constraints of the studies;therefore,further detailed studies are needed.展开更多
In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism....In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism.The research shows that coal dust surface structures,chemical and element compositions are changed in the refining process of coal dust.Compared with large particle coal dust,respirable coal dust is a weak hydrophilic and strong hydrophobic substance.The reason:the smaller the particle size of coal dust,the more unsaturated its surface.The absorption of air is strong and it is easy to form an air film on coal dust surfaces. Coal dust contact with water is actually a compound contact of liquid in contact with a solid and gas in contact with a solid. The smaller the particle size,the larger the pore volume and the higher the degree of surface roughness,the larger the area of gas in contact with a solid and the worse the coal dust wettability.Another reason is that the changes in the composition of atoms on the dust surface and structures affect coal dust wettability.The results of this study establish a theoretical basis for the invention of effective reduced-dust technology.展开更多
Testing was completed on an earlier roof bolter CAC that used slots to provide a perimeter airflow. NIOSH tested it due to its unique design that differed from canopies that provided uniform airflow. Based upon NIOSH ...Testing was completed on an earlier roof bolter CAC that used slots to provide a perimeter airflow. NIOSH tested it due to its unique design that differed from canopies that provided uniform airflow. Based upon NIOSH recommendations from the earlier testing, a 3rd generation roof bolter CAC has been developed by J.H. Fletcher & Co. The changes to this CAC involve design modifications to the plenum outlets, using a single row of outlets on the perimeter and a different material for the plenum. This laboratory testing was a continuation of the original perimeter slotted CAC design. Using gravimetric and instantaneous sampling of respirable dust concentrations underneath and outside of the CAC. the laboratory testing was completed using three different blower fans that delivered differing airflows. The maximum plenum airflow velocities ranged from 2.34 to 3.64 m/s (460-716 fpm). Results showed plenum respirable dust concentrations ranging from 34.6% to 49.3% lower than respirable dust concentrations outside the plenum protection zone, thus showing an improvement in protection for the roof bolter operators.展开更多
Dust suppression in coal mines is a worldwide problem which has not been solved effectively. The applica-tion of negative pressure secondary dust removal (NPSDR) is a breakthrough in the coal mine safety field. In thi...Dust suppression in coal mines is a worldwide problem which has not been solved effectively. The applica-tion of negative pressure secondary dust removal (NPSDR) is a breakthrough in the coal mine safety field. In this paper,NPSDR technology and ultrasonic dust suppression systems are introduced. High pressure water is supplied to the NPSDR device which is mounted on the shearer. A negative pressure field is formed in the device. At the same time,the dusty air around the shearer drum will be sucked into,and purged from,the NPSDR device by the negative pressure field. An ultrasonic dust suppression system uses water and compressed air to produce micron sized droplets which suppress respirable coal dust effectively. The NPSDR technology can be used for shearer dust suppression while ultra-sonic dust suppression can be applied in areas such as the transportation positions. These dust suppression methods have the following advantages: high efficiency,wide applicability,simple structure,high reliability and low cost.展开更多
Dust generated from bolt hole drilling in roof bolting operation could have high quartz content. As a dust control measure, vacuum drilling is employed on most of the roof bolters in US underground mines. However, fin...Dust generated from bolt hole drilling in roof bolting operation could have high quartz content. As a dust control measure, vacuum drilling is employed on most of the roof bolters in US underground mines. However, fine rock partic- ulates from drilling could escape from the dust collection system and become airborne under some circumstances causing the roof bolter operators expose to quartz-rich respirable dust. A previous research shows that drilling can be controlled through properly selected penetration and rotational rates to reduce the specific energy of drilling. Less specific energy means less energy is wasted on generating noise, heat and over-breakage of rock. It implies that proper control of drilling has a great potential to generate significantly less fine rock dust during drilling. The drilling experiments have been conducted to study the effect of controlling drilling on reducing respirable dust. The preliminary results show that the size distributions of respirable dust were different when controlling drilling in different bite depths. This paper presents the findings from laboratory experimental studies.展开更多
A 3rd generation roof bolter canopy air curtain(CAC)has been developed and constructed by J.H.Fletcher&Co.,Inc.As with the previous generation of the CAC,this design uses the principle of providing uniform airflow...A 3rd generation roof bolter canopy air curtain(CAC)has been developed and constructed by J.H.Fletcher&Co.,Inc.As with the previous generation of the CAC,this design uses the principle of providing uniform airflow across the canopy area as recommended by the National Institute for Occupational Safety and Health.The new modifications include a plenum that is constructed of a single flat aluminum plate,smaller-diameter airflow openings,and a single row of perimeter nozzles designed to prevent mine air contaminated by respirable dust from entering the CAC protection zone.Field testing was conducted on this new 3rd generation design showing reductions in coal mine respirable dust exposure for roof bolter operators.Dust control efficiencies for the CAC for the left bolter operator(intake side)ranged from approximately 26%–60%,while the efficiencies for the CAC for the right bolter operator(return side)ranged from 3%to 47%.展开更多
A 2nd generation roof bolter canopy air curtain(CAC)design was tested by National Institute for Occupational Safety and Health(NIOSH)at a Midwestern underground coal mine.During the study,the roof bolter never operate...A 2nd generation roof bolter canopy air curtain(CAC)design was tested by National Institute for Occupational Safety and Health(NIOSH)at a Midwestern underground coal mine.During the study,the roof bolter never operated downwind of the continuous miner.Using a combination of personal Data Rams(pDR)and gravimetric samplers,the dust control efficiency of the roof bolter CAC was ascertained.Performance evaluation was determined using three methods:(1)comparing roof bolter operator concentrations underneath the CAC to roof bolter concentrations outside the CAC,(2)comparing roof bolter operator concentrations underneath the CAC to the concentrations at the rear of the bolter,and finally,(3)using the gravimetric data directly underneath the CAC to correct roof bolter operator concentrations underneath the CAC and comparing them to the concentrations at the rear of the bolter.Method 1 dust control efficiencies ranged from 53.9%to 60.4%.Method 2 efficiencies ranged from 150.5%to 52.2%,and Method 3 efficiencies ranged from 40.7%to 91%.Reasons for negative and low dust control efficiencies are provided in this paper and include:incorrect sampling locations,large distance between CAC and operator,and contamination of intake air from line curtain.Low dust concentrations encountered during the testing made it difficult to discern whether differences in concentrations were due to the CAC or due to variances inherent in experimental dust measurement.However,the analyses,especially the Method 3 analysis,show that the CAC can be an effective dust control device.展开更多
Despite international efforts to limit worker exposure to coal dust,it continues to impact the health of thousands of miners across Europe.Airborne coal dust has been studied to improve risk models and its control to ...Despite international efforts to limit worker exposure to coal dust,it continues to impact the health of thousands of miners across Europe.Airborne coal dust has been studied to improve risk models and its control to protect workers.Particle size distribution analyses shows that using spraying systems to suppress airborne dusts can reduce particulate matter concentrations and that coals with higher ash yields produce finer dust.There are marked chemical differences between parent coals and relatively coarse deposited dusts(up to _(500)μm,DD_(500)).Enrichments in Ca,K,Ba,Se,Pb,Cr,Mo,Ni and especially As,Sn,Cu,Zn and Sb in the finest respirable dust fractions could originate from:(i)mechanical machinery wear;(ii)variations in coal mineralogy;(iii)coal fly ash used in shotcrete,and carbonates used to reduce the risk of explosions.Unusual enrichments in Ca in mine dusts are attributed to the use of such concrete,and elevated K to raised levels of phyllosilicate mineral matter.Sulphur concentrations are higher in the parent coal than in the DD_(500),probably due to relatively lower levels of organic matter.Mass concentrations of all elements observed in this study remained below occupational exposure limits.展开更多
The drilling operation in the roof bolting process,especially in hard rock,generates excessive respirable coal and quartz dusts,which could expose the roof bolting operator to continued health risks.Previous research ...The drilling operation in the roof bolting process,especially in hard rock,generates excessive respirable coal and quartz dusts,which could expose the roof bolting operator to continued health risks.Previous research has shown that the amount of respirable dust produced is dependent on the main drilling parameters,specifically the drilling rotational and penetration rate.In this paper,a roof bolter drilling control process was proposed to reduce the generation of respirable dust.Based on the analysis of laboratory drilling test results,a rational drilling control process(adjusting rotational and penetration rates)to achieve the optimal drilling parameter for different rock types was proposed.In this process,the ratio between specific energy and rock uniaxial compressive strength was used as the index to determine the optimal operation point.The recommended drilling operation range for the rock type used in the experiment was provided,and the reduction in respirable dust generation was demonstrated.By following this control process,the drilling efficiency can be monitored in real time,so the system can stay in a relatively high-energy efficiency with less respirable dust production from the drilling source.This algorithm is targeted to be incorporated into the current roof bolter drilling control system for drilling automation so that a safe and productive drilling operation can be conducted in a healthy working environment.展开更多
The aerodynamic diameter of an air borne particle is the key property in determining its respiratory deposition. The study aim to assess PM2.5 level and its size distribution at 5 traffic junctions located along the l...The aerodynamic diameter of an air borne particle is the key property in determining its respiratory deposition. The study aim to assess PM2.5 level and its size distribution at 5 traffic junctions located along the lateral highway connected to NH-202. A cascade impactor has been used to measure the size function range of PM2.5 apart from PM10 of atmospheric dust particles in air being PM2.5 is concern with respect to effect on human health and is able to tend deeply into the respiratory tract reaching the lungs. It is observed that weight % of PM2.5 values are in the range of 40% - 60% of PM10 and few values of PM2.5 are exceeding the standards prescribed by CPCB. It is concluded that free flow of traffic is main concern and maintenance of road should be carried out during low traffic hours.展开更多
The paper analyzed the concentrations of trace metals in fine and respirable particulates (fine-PM<sub>1</sub> and PM<sub>2.5</sub>;respirable-PM<sub>10</sub>) to determine baseline...The paper analyzed the concentrations of trace metals in fine and respirable particulates (fine-PM<sub>1</sub> and PM<sub>2.5</sub>;respirable-PM<sub>10</sub>) to determine baseline concentrations in the ambient air and the factors impacting its distribution such as land use and time of year when levels may be concerning to public health. Measurements of particulates along with meteorological parameters were made at four sites over the heavily populated west coast of Trinidad (10<span style="font-size:10.0pt;font-family:;" "=""><span style="color:#4F4F4F;font-family:"font-size:14px;white-space:normal;background-color:#FFFFFF;">°</span></span>32'N, 61<span style="font-size:10.0pt;font-family:;" "=""><span style="color:#4F4F4F;font-family:"font-size:14px;white-space:normal;background-color:#FFFFFF;">°</span></span>18'W) during March ’15-May ’16, representing rural, urban, mixed background and industrial land uses. The study found mean levels of trace metals to be highest at the industrial and urban stations. Public health exceedances (referenced to the Canadian AAQ public health standards <a href="#ref23">(Ontario-MoE, 2012)</a>) were measured for beryllium, cadmium, chromium, iron, manganese and nickel (in PM<sub>10</sub>). Iron, manganese and nickel, most associated with particulates at the industrial station, were in frequent exceedance. Beryllium—concentrated in coarse PM (PM<sub>2.5-10</sub>) with only a single measured exceedance at the mixed background station likely poses minimal threat to the health of the nearby population. Cadmium—concentrated in fine PM which peaked once only at the rural station was likely due to an irregular event within a narrow timeframe during the time of sampling. Iron and manganese were frequently above the Canadian public health threshold, but predominated in the coarse PM fraction, suggesting localised sources. Nickel, concentrated in the fine PM fraction, was frequently in exceedance particularly at the industrial station. Cadmium and nickel are genotoxic and should be regulated in order to reduce the burden of toxic carcinogens to which the population can be exposed.展开更多
The concentration of respirable dust at seven sampling stations in Jos metropolitan area, was measured weekly for three consecutive months (October 2012 to December 2012). Characterization of the dust particles shows ...The concentration of respirable dust at seven sampling stations in Jos metropolitan area, was measured weekly for three consecutive months (October 2012 to December 2012). Characterization of the dust particles shows that the concentration of fine dust ( μm) ranged from 55.0 - 124.9 μg/m3 while the coarse dust (2.5 - 10 μm) varied from 20.0 - 124.4 μg/m3. FAAS analysis of the respirable dust shows that the concentrations of heavy metals ranged from 0.121 - 0.832 mg/kg Pb, 0.019 - 0.111 mg/kg Cr, 0.171 - 1.081 mg/kg Fe, 0.002 - 0.056 mg/kg Mn, 0.002 - 0.438 mg/kg Cd, 0.696 - 1.712 mg/kg Zn, 0.025 - 0.571 mg/kg Cu, and 0.021 - 0. 478 mg/kg Ni, across the sites studied. The main sources of these heavy metals in the sampled area could be attributed to anthropogenic activities like open incineration of waste and vehicular traffic. The concentrations of Cd, Mn and Ni were found to be far above the standard limits prescribed by the WHO for respirable dust. These results convey the health risk the inhabitants in the study area are exposed to. We therefore recommend that measures be taken to regulate these anthropogenic展开更多
In order to understand the migration law of respirable dust and gain reasonable design parameters for dust control on a super-long double-shearer fully mechanized working face, this paper describes research carried ou...In order to understand the migration law of respirable dust and gain reasonable design parameters for dust control on a super-long double-shearer fully mechanized working face, this paper describes research carried out using a numerical simulation package(Fluent) based on gas-solid coupling dispersed multiphase flow model and field measurement to research different technology modes, dust distribution law at different intervals where shearers work in opposite directions on the lower 9303 face, No. 2 Jining Mine,Yankuang Coal Mining Co. Results show that the concentration of dust 3–6 m away from the shearers working in the same directions was large, while the impact area of respirable dust near the shearer increased significantly to 5–6 m with the distance between two shearers working in opposite directions.The concentration of dust on a double-shearer face was considerably higher than that of a face with one shear under the combined effect of wind speed on the face and disturbed wind around the shearer, while the dust concentration near the shearer on the return side was considerably higher than that on the inlet side. The concentration of dust on a double-shearer face along the airflow declined slowly so that dust was hard to control. Simulation results confirmed the results of field measurement, which could provide reference for dust prevention design.展开更多
Introduction: Prosperity in the mining industry in Mongolia has led to an increase in the number of mining workers, resulting in an increase in occupational exposures and the prevalence of occupational diseases and di...Introduction: Prosperity in the mining industry in Mongolia has led to an increase in the number of mining workers, resulting in an increase in occupational exposures and the prevalence of occupational diseases and disabilities. The aim of this study was to assess the personal exposure of workers to respirable dust (RD) and respirable crystalline silica (RCS). Method: A cross-sectional study was conducted at the Erdenet Copper Ore Mining and included 581 personal samples of RD and 324 samples of RCS, collected in 2018-2020. Results: The study results revealed that the geometric mean (GM) of RD was 0.35 mg/m<sup>3</sup> (95% CI 0.32 - 0.39). The RD concentration in 5.9% (n = 34) of all the samples exceeded the permissible exposure level (PEL) (3 mg/mg<sup>3</sup>). The GM of RCS was 0.012 mg/m<sup>3</sup> (95% CI 0.010 - 0.016). In total, 12.0% (n = 39) of all RCS sample concentrations exceeded the PEL (0.1 mg/m<sup>3</sup>). Conclusion: Steel smelters, blasters, welders, and maintenance laborers were exposed to RD, while crushers, operators, drilling machinists, and maintenance laborers were exposed to RCS. Therefore, regular monitoring of the work environment, risk-based health examinations for dust-exposed workers, efficient support for dust mitigation, reduction measures at the source, and engineering controls are needed.展开更多
Airborne rock dust poses serious long-term health effects to workers in mining and tunneling underground rock environments.When inhaled,respirable crystalline silica particles commonly found in quartz and other minera...Airborne rock dust poses serious long-term health effects to workers in mining and tunneling underground rock environments.When inhaled,respirable crystalline silica particles commonly found in quartz and other minerals will scar sensitive lung tissue and cause irreversible lung diseases.Characteristics such as concentration,type of mineral,particle size,and particle shape can harm workers to various extents.Therefore,this study characterizes airborne rock dust particles that are released from mechanically cutting rock.Laboratory full scale linear cutting tests on samples of potash rock were performed with radial picks to generate dust and were collected with various instruments,including Dorr-Oliver cyclones.Three stages of pick wear were tested:new,moderately worn,and severely worn.Comparisons between different stages of pick wear to dust concentration,size distribution,and particle shape characteristics are drawn from this preliminary study using analytical methods,field-emission scanning electron microscope image capture techniques,and laser diffraction.Although further testing needs to be conducted to make viable and concrete conclusions,a review of the test results reveals a strong tendency in generated airborne and deposited dust to be linked to the bit tip wear,which was influenced by tip surface area geometry,such as a sharp tip,blunt tip,or undulating sharp tips.The moderately worn pick,or the pick with the bluntest tip,in these experiments released the highest concentration of dust.The moderately worn(bluntest)pick also generated particle shapes with the highest aspect ratio compared to the other two picks.Additionally,in terms of the particle size distributions,all the picks generated airborne particle size mean values between 0.7 and 1.2µm in aerodynamic diameter.As for deposited particle size distributions,all the picks generated particles with the mode of particle aerodynamic diameter sizes at 13µm.In the end,the results of this preliminary study paired with future testing can confirm and eventually provide the basis for optimum bit management and maintenance systems to control airborne dust exposures.展开更多
Apparel processing is an essential industry in providing clothing needs for the population. The Export Processing Zone (EPZ) in Kenya employs many employees. Garment processing releases respirable dust particles, thus...Apparel processing is an essential industry in providing clothing needs for the population. The Export Processing Zone (EPZ) in Kenya employs many employees. Garment processing releases respirable dust particles, thus exposing workers to risks to the respiratory system. The study determined the respirable dust health concerns among workers in Apparel Processing Companies (APCs) in EPZ in Machakos County, Kenya. A cross-sectional descriptive design was employed where four companies were studied. Three hundred and sixty-seven participants were selected through systematic random sampling. Data was collected using questionnaires and Interview guides. The study established that workers were exposed to respirable dust PM<sub>2.5</sub> ranging from 40.89 ± 24.0 μg·m<sup>−3</sup> to 87.49 ± 45.2 μg·m<sup>−3</sup> with a mean of 65.61 ± 31.5 μg·m<sup>−3</sup>. While PM<sub>2.5</sub> ranged from 63.59 ± 21.2 μg·m<sup>−3</sup> to 313.41 ± 468.0 μg·m<sup>−3</sup>. With a mean of 104.02 ± 26.0 μg·m<sup>−3</sup>. Workers complained of different respirable dust-related diseases. The most prevalent conditions were sneezing and coughing (86.4%), chest pains (41.1%), blocked chests (36.8%), and allergic reactions to dust (18.3%). The APC should develop an OSH management system that includes;a dust management policy, dust monitoring, Risk Assessments, Engineering controls installations, medical examination, Training on dust management, PPE provision, and use enforcement.展开更多
Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental changes.However,as a principal silvicult...Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental changes.However,as a principal silvicultural practice,the long-term impacts of thinning on R_(S) and its heterotrophic and autotrophic respiration components(R_(h) and Ra,respectively)in subalpine plantations are poorly understood,espe-cially in winter.A 3-year field observation was carried out with consideration of winter CO_(2) efflux in middle-aged sub-alpine spruce plantations in northwestern China.A trench-ing method was used to explore the long-term impacts of thinning on Rs,Rn and R_(a).Seventeen years after thinning,mean annual Rs,Rn and R_(a) increased,while the contribu-tion of R_(h) to R_(s) decreased with thinning intensity.Thinning significantly decreased winter R,because of the reduction in R_(n) but had no significant effect on Ra.The temperature sensitivity(Q_(10))of R_(h) and R_(a) also increased with thinning intensity,with lower Q_(10) values for R_(h)(2.1-2.6)than for Ra(2.4-2.8).The results revealed the explanatory variables and pathways related to R_(n) and R_(a) dynamics.Thinning increased soil moisture and nitrate nitrogen(NO_(3)^(-)-N),and the enhanced nitrogen and water availability promoted R_(h) and R_(a) by improving fine root biomass and microbial activity.Our results highlight the positive roles of NO_(3)^(-)-N in stimulating R_(s) components following long-term thinning.Therefore,applications of nitrogen fertilizer are not recommended while thinning subalpine spruce plantations from the perspective of reducing soil CO_(2) emissions.The increased Q_(10) values of R_(s) components indicate that a large increase in soil CO_(2) emissions would be expected following thinning because of more pronounced climate warming in alpineregions.展开更多
The function of exogenous alanine(Ala)in regulating biomass accumulation,lipid production,photosynthesis,and respiration in Chlorella pyrenoidosa was studied.Result shows that the supplementation of Ala increased C.py...The function of exogenous alanine(Ala)in regulating biomass accumulation,lipid production,photosynthesis,and respiration in Chlorella pyrenoidosa was studied.Result shows that the supplementation of Ala increased C.pyrenoidosa biomass and lipid production in an 8-d batch culture.The concentration of 10 mmol/L of Ala was optimum and increased the microalgal cell biomass and lipid content by 39.3%and 21.4%,respectively,compared with that in the control(0-mmol/L Ala).Ala supplementation reduced photosynthetic activity while boosting respiratory activity and pyruvate levels,indicating that C.pyrenoidosa used exogenous Ala for biomass accumulation through the respiratory metabolic process.The accelerated respiratory metabolism due to Ala supplementation elevated the substrate pool and improved the lipogenic gene expression,promoting lipid production at last.This study provided a novel method for increasing biomass accumulation and lipid production and elucidated the role of Ala in regulating lipid production.展开更多
基金CDC/NIOSH for funding this research(75D30119C05529)。
文摘Control of dust in underground coal mines is critical for mitigating both safety and health hazards.For decades,the National Institute of Occupational Safety and Health(NIOSH)has led research to evaluate the effectiveness of various dust control technologies in coal mines.Recent studies have included the evaluation of auxiliary scrubbers to reduce respirable dust downstream of active mining and the use of canopy air curtains(CACs)to reduce respirable dust in key operator positions.While detailed dust characterization was not a focus of such studies,this is a growing area of interest.Using preserved filter samples from three previous NIOSH studies,the current work aims to explore the effect of two different scrubbers(one wet and one dry)and a roof bolter CAC on respirable dust composition and particle size distribution.For this,the preserved filter samples were analyzed by thermogravimetric analysis and/or scanning electron microscopy with energy dispersive X-ray.Results indicate that dust composition was not appreciably affected by either scrubber or the CAC.However,the wet scrubber and CAC appeared to decrease the overall particle size distribution.Such an effect of the dry scrubber was not consistently observed,but this is probably related to the particular sampling location downstream of the scrubber which allowed for significant mixing of the scrubber exhaust and other return air.Aside from the insights gained with respect to the three specific dust control case studies revisited here,this work demonstrates the value of preserved dust samples for follow-up investigation more broadly.
基金supported by the Alpha Foundation for the Improvement of Mine Safety and Health,grant number AFC316FO-84.
文摘Exposure to respirable coal mine dust(RCMD)can cause chronic and debilitating lung diseases.Real-time monitoring capabilities are sought which can enable a better understanding of dust components and sources.In many underground mines,RCMD includes three primary components which can be loosely associated with three major dust sources:coal dust from the coal seam itself,silicates from the surrounding rock strata,and carbonates from the inert‘rock dust’products that are applied to mitigate explosion hazards.A monitor which can reliably partition RCMD between these three components could thus allow source apportionment.And tracking silicates,specifically,could be valuable since the most serious health risks are typically associated with this component-particularly if abundant in crystalline silica.Envisioning a monitoring concept based on field microscopy,and following up on prior research using polarized light,the aim of the current study was to build and test a model to classify respirable-sized particles as either coal,silicates,or carbonates.For model development,composite dust samples were generated in the laboratory by successively depositing dust from high-purity materials onto a sticky transparent substrate,and imaging after each deposition event such that the identity of each particle was known a priori.Model testing followed a similar approach,except that real geologic materials were used as the source for each dust component.Results showed that the model had an overall accuracy of 86.5%,indicating that a field-microscopy based moni-tor could support RCMD source apportionment and silicates tracking in some coal mines.
基金funded by the National Institute for Occupational Safety and Health (NIOSH) (75D30119C06390).
文摘Cumulative inhalation of respirable coal mine dust(RCMD)can lead to severe lung diseases,including coal worker's pneu-moconiosis(CWP),silicosis,mixed dust pneumoconiosis,dust-related diffuse fibrosis(DDF),and progressive massive fibrosis(PMF).Statistics from the number of reported cases showed a significant decrease in the progression of respiratory diseases in the 1990s.However,an unexpected increase in the number of CWP cases was reported in the late 1990s.To date,there has been no comprehensive systematic review to assess all contributing factors to the resurgence of CWP cases.This study aims to investigate the effects of various mining parameters on the prevalence of CWP in coal mines.A systematic review using the preferred reporting items for systematic reviews and meta-analysis(PRISMA)method was conducted to investigate the health effects of RCMD exposure and identify the factors that may contribute to the recent resurgence of CWP cases.The systematic review yielded a total of 401 papers,which were added to the database.The total number of 148 and 208 papers were excluded from the database in the process of screening and eligibility,respectively.Then,18 papers were considered for data selection and full-text assessment.The review revealed that factors including geographic location,mine size,mining operation type,coal-seam thickness,coal rank,changes in mining practices,technology advancement,and engi-neering dust control practices are contributing to the recent resurgence of CWP among coal workers.However,the evidence for root causes is limited owing to the methodological constraints of the studies;therefore,further detailed studies are needed.
基金Financial support for this work,provided by the Science and Technology Project of the Education Department of Shandong Province(No.J08LC12),
文摘In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism.The research shows that coal dust surface structures,chemical and element compositions are changed in the refining process of coal dust.Compared with large particle coal dust,respirable coal dust is a weak hydrophilic and strong hydrophobic substance.The reason:the smaller the particle size of coal dust,the more unsaturated its surface.The absorption of air is strong and it is easy to form an air film on coal dust surfaces. Coal dust contact with water is actually a compound contact of liquid in contact with a solid and gas in contact with a solid. The smaller the particle size,the larger the pore volume and the higher the degree of surface roughness,the larger the area of gas in contact with a solid and the worse the coal dust wettability.Another reason is that the changes in the composition of atoms on the dust surface and structures affect coal dust wettability.The results of this study establish a theoretical basis for the invention of effective reduced-dust technology.
文摘Testing was completed on an earlier roof bolter CAC that used slots to provide a perimeter airflow. NIOSH tested it due to its unique design that differed from canopies that provided uniform airflow. Based upon NIOSH recommendations from the earlier testing, a 3rd generation roof bolter CAC has been developed by J.H. Fletcher & Co. The changes to this CAC involve design modifications to the plenum outlets, using a single row of outlets on the perimeter and a different material for the plenum. This laboratory testing was a continuation of the original perimeter slotted CAC design. Using gravimetric and instantaneous sampling of respirable dust concentrations underneath and outside of the CAC. the laboratory testing was completed using three different blower fans that delivered differing airflows. The maximum plenum airflow velocities ranged from 2.34 to 3.64 m/s (460-716 fpm). Results showed plenum respirable dust concentrations ranging from 34.6% to 49.3% lower than respirable dust concentrations outside the plenum protection zone, thus showing an improvement in protection for the roof bolter operators.
文摘Dust suppression in coal mines is a worldwide problem which has not been solved effectively. The applica-tion of negative pressure secondary dust removal (NPSDR) is a breakthrough in the coal mine safety field. In this paper,NPSDR technology and ultrasonic dust suppression systems are introduced. High pressure water is supplied to the NPSDR device which is mounted on the shearer. A negative pressure field is formed in the device. At the same time,the dusty air around the shearer drum will be sucked into,and purged from,the NPSDR device by the negative pressure field. An ultrasonic dust suppression system uses water and compressed air to produce micron sized droplets which suppress respirable coal dust effectively. The NPSDR technology can be used for shearer dust suppression while ultra-sonic dust suppression can be applied in areas such as the transportation positions. These dust suppression methods have the following advantages: high efficiency,wide applicability,simple structure,high reliability and low cost.
文摘Dust generated from bolt hole drilling in roof bolting operation could have high quartz content. As a dust control measure, vacuum drilling is employed on most of the roof bolters in US underground mines. However, fine rock partic- ulates from drilling could escape from the dust collection system and become airborne under some circumstances causing the roof bolter operators expose to quartz-rich respirable dust. A previous research shows that drilling can be controlled through properly selected penetration and rotational rates to reduce the specific energy of drilling. Less specific energy means less energy is wasted on generating noise, heat and over-breakage of rock. It implies that proper control of drilling has a great potential to generate significantly less fine rock dust during drilling. The drilling experiments have been conducted to study the effect of controlling drilling on reducing respirable dust. The preliminary results show that the size distributions of respirable dust were different when controlling drilling in different bite depths. This paper presents the findings from laboratory experimental studies.
文摘A 3rd generation roof bolter canopy air curtain(CAC)has been developed and constructed by J.H.Fletcher&Co.,Inc.As with the previous generation of the CAC,this design uses the principle of providing uniform airflow across the canopy area as recommended by the National Institute for Occupational Safety and Health.The new modifications include a plenum that is constructed of a single flat aluminum plate,smaller-diameter airflow openings,and a single row of perimeter nozzles designed to prevent mine air contaminated by respirable dust from entering the CAC protection zone.Field testing was conducted on this new 3rd generation design showing reductions in coal mine respirable dust exposure for roof bolter operators.Dust control efficiencies for the CAC for the left bolter operator(intake side)ranged from approximately 26%–60%,while the efficiencies for the CAC for the right bolter operator(return side)ranged from 3%to 47%.
文摘A 2nd generation roof bolter canopy air curtain(CAC)design was tested by National Institute for Occupational Safety and Health(NIOSH)at a Midwestern underground coal mine.During the study,the roof bolter never operated downwind of the continuous miner.Using a combination of personal Data Rams(pDR)and gravimetric samplers,the dust control efficiency of the roof bolter CAC was ascertained.Performance evaluation was determined using three methods:(1)comparing roof bolter operator concentrations underneath the CAC to roof bolter concentrations outside the CAC,(2)comparing roof bolter operator concentrations underneath the CAC to the concentrations at the rear of the bolter,and finally,(3)using the gravimetric data directly underneath the CAC to correct roof bolter operator concentrations underneath the CAC and comparing them to the concentrations at the rear of the bolter.Method 1 dust control efficiencies ranged from 53.9%to 60.4%.Method 2 efficiencies ranged from 150.5%to 52.2%,and Method 3 efficiencies ranged from 40.7%to 91%.Reasons for negative and low dust control efficiencies are provided in this paper and include:incorrect sampling locations,large distance between CAC and operator,and contamination of intake air from line curtain.Low dust concentrations encountered during the testing made it difficult to discern whether differences in concentrations were due to the CAC or due to variances inherent in experimental dust measurement.However,the analyses,especially the Method 3 analysis,show that the CAC can be an effective dust control device.
基金European Commission Research Fund for Coal and Steel(Grant Agreement Number–754205)Generalitat de Catalunya(SGR41).Centre of Excellence Severo Ochoa—Spanish Ministry of Science and Innovation(Project CEX2018-000794-S).
文摘Despite international efforts to limit worker exposure to coal dust,it continues to impact the health of thousands of miners across Europe.Airborne coal dust has been studied to improve risk models and its control to protect workers.Particle size distribution analyses shows that using spraying systems to suppress airborne dusts can reduce particulate matter concentrations and that coals with higher ash yields produce finer dust.There are marked chemical differences between parent coals and relatively coarse deposited dusts(up to _(500)μm,DD_(500)).Enrichments in Ca,K,Ba,Se,Pb,Cr,Mo,Ni and especially As,Sn,Cu,Zn and Sb in the finest respirable dust fractions could originate from:(i)mechanical machinery wear;(ii)variations in coal mineralogy;(iii)coal fly ash used in shotcrete,and carbonates used to reduce the risk of explosions.Unusual enrichments in Ca in mine dusts are attributed to the use of such concrete,and elevated K to raised levels of phyllosilicate mineral matter.Sulphur concentrations are higher in the parent coal than in the DD_(500),probably due to relatively lower levels of organic matter.Mass concentrations of all elements observed in this study remained below occupational exposure limits.
文摘The drilling operation in the roof bolting process,especially in hard rock,generates excessive respirable coal and quartz dusts,which could expose the roof bolting operator to continued health risks.Previous research has shown that the amount of respirable dust produced is dependent on the main drilling parameters,specifically the drilling rotational and penetration rate.In this paper,a roof bolter drilling control process was proposed to reduce the generation of respirable dust.Based on the analysis of laboratory drilling test results,a rational drilling control process(adjusting rotational and penetration rates)to achieve the optimal drilling parameter for different rock types was proposed.In this process,the ratio between specific energy and rock uniaxial compressive strength was used as the index to determine the optimal operation point.The recommended drilling operation range for the rock type used in the experiment was provided,and the reduction in respirable dust generation was demonstrated.By following this control process,the drilling efficiency can be monitored in real time,so the system can stay in a relatively high-energy efficiency with less respirable dust production from the drilling source.This algorithm is targeted to be incorporated into the current roof bolter drilling control system for drilling automation so that a safe and productive drilling operation can be conducted in a healthy working environment.
文摘The aerodynamic diameter of an air borne particle is the key property in determining its respiratory deposition. The study aim to assess PM2.5 level and its size distribution at 5 traffic junctions located along the lateral highway connected to NH-202. A cascade impactor has been used to measure the size function range of PM2.5 apart from PM10 of atmospheric dust particles in air being PM2.5 is concern with respect to effect on human health and is able to tend deeply into the respiratory tract reaching the lungs. It is observed that weight % of PM2.5 values are in the range of 40% - 60% of PM10 and few values of PM2.5 are exceeding the standards prescribed by CPCB. It is concluded that free flow of traffic is main concern and maintenance of road should be carried out during low traffic hours.
文摘The paper analyzed the concentrations of trace metals in fine and respirable particulates (fine-PM<sub>1</sub> and PM<sub>2.5</sub>;respirable-PM<sub>10</sub>) to determine baseline concentrations in the ambient air and the factors impacting its distribution such as land use and time of year when levels may be concerning to public health. Measurements of particulates along with meteorological parameters were made at four sites over the heavily populated west coast of Trinidad (10<span style="font-size:10.0pt;font-family:;" "=""><span style="color:#4F4F4F;font-family:"font-size:14px;white-space:normal;background-color:#FFFFFF;">°</span></span>32'N, 61<span style="font-size:10.0pt;font-family:;" "=""><span style="color:#4F4F4F;font-family:"font-size:14px;white-space:normal;background-color:#FFFFFF;">°</span></span>18'W) during March ’15-May ’16, representing rural, urban, mixed background and industrial land uses. The study found mean levels of trace metals to be highest at the industrial and urban stations. Public health exceedances (referenced to the Canadian AAQ public health standards <a href="#ref23">(Ontario-MoE, 2012)</a>) were measured for beryllium, cadmium, chromium, iron, manganese and nickel (in PM<sub>10</sub>). Iron, manganese and nickel, most associated with particulates at the industrial station, were in frequent exceedance. Beryllium—concentrated in coarse PM (PM<sub>2.5-10</sub>) with only a single measured exceedance at the mixed background station likely poses minimal threat to the health of the nearby population. Cadmium—concentrated in fine PM which peaked once only at the rural station was likely due to an irregular event within a narrow timeframe during the time of sampling. Iron and manganese were frequently above the Canadian public health threshold, but predominated in the coarse PM fraction, suggesting localised sources. Nickel, concentrated in the fine PM fraction, was frequently in exceedance particularly at the industrial station. Cadmium and nickel are genotoxic and should be regulated in order to reduce the burden of toxic carcinogens to which the population can be exposed.
文摘The concentration of respirable dust at seven sampling stations in Jos metropolitan area, was measured weekly for three consecutive months (October 2012 to December 2012). Characterization of the dust particles shows that the concentration of fine dust ( μm) ranged from 55.0 - 124.9 μg/m3 while the coarse dust (2.5 - 10 μm) varied from 20.0 - 124.4 μg/m3. FAAS analysis of the respirable dust shows that the concentrations of heavy metals ranged from 0.121 - 0.832 mg/kg Pb, 0.019 - 0.111 mg/kg Cr, 0.171 - 1.081 mg/kg Fe, 0.002 - 0.056 mg/kg Mn, 0.002 - 0.438 mg/kg Cd, 0.696 - 1.712 mg/kg Zn, 0.025 - 0.571 mg/kg Cu, and 0.021 - 0. 478 mg/kg Ni, across the sites studied. The main sources of these heavy metals in the sampled area could be attributed to anthropogenic activities like open incineration of waste and vehicular traffic. The concentrations of Cd, Mn and Ni were found to be far above the standard limits prescribed by the WHO for respirable dust. These results convey the health risk the inhabitants in the study area are exposed to. We therefore recommend that measures be taken to regulate these anthropogenic
基金the National Natural Science Foundation of China (No.51404249)the Basic Research Program of Jiangsu Province (No.BK20140201)the Priority Academic Program Development of Jiangsu Higher Education Institutions for financial support provided during this research
文摘In order to understand the migration law of respirable dust and gain reasonable design parameters for dust control on a super-long double-shearer fully mechanized working face, this paper describes research carried out using a numerical simulation package(Fluent) based on gas-solid coupling dispersed multiphase flow model and field measurement to research different technology modes, dust distribution law at different intervals where shearers work in opposite directions on the lower 9303 face, No. 2 Jining Mine,Yankuang Coal Mining Co. Results show that the concentration of dust 3–6 m away from the shearers working in the same directions was large, while the impact area of respirable dust near the shearer increased significantly to 5–6 m with the distance between two shearers working in opposite directions.The concentration of dust on a double-shearer face was considerably higher than that of a face with one shear under the combined effect of wind speed on the face and disturbed wind around the shearer, while the dust concentration near the shearer on the return side was considerably higher than that on the inlet side. The concentration of dust on a double-shearer face along the airflow declined slowly so that dust was hard to control. Simulation results confirmed the results of field measurement, which could provide reference for dust prevention design.
文摘Introduction: Prosperity in the mining industry in Mongolia has led to an increase in the number of mining workers, resulting in an increase in occupational exposures and the prevalence of occupational diseases and disabilities. The aim of this study was to assess the personal exposure of workers to respirable dust (RD) and respirable crystalline silica (RCS). Method: A cross-sectional study was conducted at the Erdenet Copper Ore Mining and included 581 personal samples of RD and 324 samples of RCS, collected in 2018-2020. Results: The study results revealed that the geometric mean (GM) of RD was 0.35 mg/m<sup>3</sup> (95% CI 0.32 - 0.39). The RD concentration in 5.9% (n = 34) of all the samples exceeded the permissible exposure level (PEL) (3 mg/mg<sup>3</sup>). The GM of RCS was 0.012 mg/m<sup>3</sup> (95% CI 0.010 - 0.016). In total, 12.0% (n = 39) of all RCS sample concentrations exceeded the PEL (0.1 mg/m<sup>3</sup>). Conclusion: Steel smelters, blasters, welders, and maintenance laborers were exposed to RD, while crushers, operators, drilling machinists, and maintenance laborers were exposed to RCS. Therefore, regular monitoring of the work environment, risk-based health examinations for dust-exposed workers, efficient support for dust mitigation, reduction measures at the source, and engineering controls are needed.
文摘Airborne rock dust poses serious long-term health effects to workers in mining and tunneling underground rock environments.When inhaled,respirable crystalline silica particles commonly found in quartz and other minerals will scar sensitive lung tissue and cause irreversible lung diseases.Characteristics such as concentration,type of mineral,particle size,and particle shape can harm workers to various extents.Therefore,this study characterizes airborne rock dust particles that are released from mechanically cutting rock.Laboratory full scale linear cutting tests on samples of potash rock were performed with radial picks to generate dust and were collected with various instruments,including Dorr-Oliver cyclones.Three stages of pick wear were tested:new,moderately worn,and severely worn.Comparisons between different stages of pick wear to dust concentration,size distribution,and particle shape characteristics are drawn from this preliminary study using analytical methods,field-emission scanning electron microscope image capture techniques,and laser diffraction.Although further testing needs to be conducted to make viable and concrete conclusions,a review of the test results reveals a strong tendency in generated airborne and deposited dust to be linked to the bit tip wear,which was influenced by tip surface area geometry,such as a sharp tip,blunt tip,or undulating sharp tips.The moderately worn pick,or the pick with the bluntest tip,in these experiments released the highest concentration of dust.The moderately worn(bluntest)pick also generated particle shapes with the highest aspect ratio compared to the other two picks.Additionally,in terms of the particle size distributions,all the picks generated airborne particle size mean values between 0.7 and 1.2µm in aerodynamic diameter.As for deposited particle size distributions,all the picks generated particles with the mode of particle aerodynamic diameter sizes at 13µm.In the end,the results of this preliminary study paired with future testing can confirm and eventually provide the basis for optimum bit management and maintenance systems to control airborne dust exposures.
文摘Apparel processing is an essential industry in providing clothing needs for the population. The Export Processing Zone (EPZ) in Kenya employs many employees. Garment processing releases respirable dust particles, thus exposing workers to risks to the respiratory system. The study determined the respirable dust health concerns among workers in Apparel Processing Companies (APCs) in EPZ in Machakos County, Kenya. A cross-sectional descriptive design was employed where four companies were studied. Three hundred and sixty-seven participants were selected through systematic random sampling. Data was collected using questionnaires and Interview guides. The study established that workers were exposed to respirable dust PM<sub>2.5</sub> ranging from 40.89 ± 24.0 μg·m<sup>−3</sup> to 87.49 ± 45.2 μg·m<sup>−3</sup> with a mean of 65.61 ± 31.5 μg·m<sup>−3</sup>. While PM<sub>2.5</sub> ranged from 63.59 ± 21.2 μg·m<sup>−3</sup> to 313.41 ± 468.0 μg·m<sup>−3</sup>. With a mean of 104.02 ± 26.0 μg·m<sup>−3</sup>. Workers complained of different respirable dust-related diseases. The most prevalent conditions were sneezing and coughing (86.4%), chest pains (41.1%), blocked chests (36.8%), and allergic reactions to dust (18.3%). The APC should develop an OSH management system that includes;a dust management policy, dust monitoring, Risk Assessments, Engineering controls installations, medical examination, Training on dust management, PPE provision, and use enforcement.
基金supported by the National Natural Science Foundation of China (Grant Nos.41701296 and 42277481)the Natural Science Foundation of Gansu Province (GrantNo.22JR5RA058)the Youth Science and Technology Fund Program of Gansu Province (Grant No.22JR5RA087).
文摘Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental changes.However,as a principal silvicultural practice,the long-term impacts of thinning on R_(S) and its heterotrophic and autotrophic respiration components(R_(h) and Ra,respectively)in subalpine plantations are poorly understood,espe-cially in winter.A 3-year field observation was carried out with consideration of winter CO_(2) efflux in middle-aged sub-alpine spruce plantations in northwestern China.A trench-ing method was used to explore the long-term impacts of thinning on Rs,Rn and R_(a).Seventeen years after thinning,mean annual Rs,Rn and R_(a) increased,while the contribu-tion of R_(h) to R_(s) decreased with thinning intensity.Thinning significantly decreased winter R,because of the reduction in R_(n) but had no significant effect on Ra.The temperature sensitivity(Q_(10))of R_(h) and R_(a) also increased with thinning intensity,with lower Q_(10) values for R_(h)(2.1-2.6)than for Ra(2.4-2.8).The results revealed the explanatory variables and pathways related to R_(n) and R_(a) dynamics.Thinning increased soil moisture and nitrate nitrogen(NO_(3)^(-)-N),and the enhanced nitrogen and water availability promoted R_(h) and R_(a) by improving fine root biomass and microbial activity.Our results highlight the positive roles of NO_(3)^(-)-N in stimulating R_(s) components following long-term thinning.Therefore,applications of nitrogen fertilizer are not recommended while thinning subalpine spruce plantations from the perspective of reducing soil CO_(2) emissions.The increased Q_(10) values of R_(s) components indicate that a large increase in soil CO_(2) emissions would be expected following thinning because of more pronounced climate warming in alpineregions.
基金Supported by the National Natural Science Foundation of China(Nos.32002411,42276189)the Innovation and Entrepreneurship Project for College Students of Hohai University(No.2022102941027)the Jiangsu Innovation Center for Marine Bioresources(No.822153216)。
文摘The function of exogenous alanine(Ala)in regulating biomass accumulation,lipid production,photosynthesis,and respiration in Chlorella pyrenoidosa was studied.Result shows that the supplementation of Ala increased C.pyrenoidosa biomass and lipid production in an 8-d batch culture.The concentration of 10 mmol/L of Ala was optimum and increased the microalgal cell biomass and lipid content by 39.3%and 21.4%,respectively,compared with that in the control(0-mmol/L Ala).Ala supplementation reduced photosynthetic activity while boosting respiratory activity and pyruvate levels,indicating that C.pyrenoidosa used exogenous Ala for biomass accumulation through the respiratory metabolic process.The accelerated respiratory metabolism due to Ala supplementation elevated the substrate pool and improved the lipogenic gene expression,promoting lipid production at last.This study provided a novel method for increasing biomass accumulation and lipid production and elucidated the role of Ala in regulating lipid production.