Quantification of In Vivo Epidermal Keratinocyte Architecture Associated with the Signs of Skin Aging and the Skin Benefit Evaluation by Application of Galactomyces Ferment Filtrate (Pitera)-Containing Skin Care Product

Background: Aged skin exhibits visual alterations such as wrinkles, rough texture, pore dilation, and dull skin tone, as well as physiological aging, namely, decreased hydration and increased transepidermal water loss (TEWL). Recent advances in coherence tomography have also revealed that skin aging affects in vivo epidermal keratinocyte architecture. However, the interconnectivity between spatial architectural aging and visual/physiological aging parameters remains largely unknown. Purpose: To elucidate whether the tomographic keratinocyte architectural aging is correlated with visual and physiological skin aging parameters and to quantitatively evaluate the improvements of the architectural, visual, and physiological aging parameters by the daily treatment of the skin care formula containing Galactomyces Ferment Filtrate (GFF, 8X PiteraTM). Method: We measured the in vivo keratinocyte cellular architecture with two-photon stereoscopic tomography obtaining by-layer epidermal section images in 78 Asian females of various ages. Visual aging parameters were analyzed using a portable image capture system. Hydration and TEWL were also assessed. The anti-aging effects of GFF-containing skin moisturizer (SK-II LXP CreamTM) were also examined in two studies after twice-daily application for 2 (N = 35) and 4 (N = 32) weeks. Results: As for the keratinocyte cellular architecture, skin aging was significantly associated with decreased cell density and increased cell uniformity. These architectural aging parameters were significantly correlated with visual and physiological aging parameters, namely, rough texture, wrinkles, pore dilation, dull skin tone, dehydration, and increased TEWL. The strong interconnectivity allowed us to develop formulae to estimate the keratinocyte architecture from visual aging parameters. Moreover, twice-daily application of SK-II significantly improved the keratinocyte architecture associated with multiple skin aging visual and physiological parameters. Conclusion: Skin aging is a process involving mutual interconnections among epidermal keratinocyte cellular architecture, visual, and physiological parameters. The GFF-containing moisturizer SK-II effectively improves spatial architecture of keratinocytes in epidermis and these evaluated skin aging parameters in a new trajectory over the course of treatment. .

RGD修饰的肿瘤抑素19肽的合成与鉴定及其对肝癌SK-Hep-1细胞增殖、迁移和侵袭的抑制作用

目的:探讨精氨酸-甘氨酸-天冬氨酸(RGD)修饰对肿瘤抑素19肽(T-19)抗肝癌活性的影响,比较分析T-19及RGD修饰的T-19(RGD-T-19)对肝癌SK-Hep-1细胞增殖、侵袭和迁移能力的影响。方法:用Fmoc固相法合成T-19及RGD-T-19,用高效液相色谱仪和质谱进行分离、鉴定。常规培养SK-Hep-1细胞,用0、50、100、150、200、250μg/mL的T-19及RGD-T-19分别处理细胞,分为0μg/mL(对照)组、50μg/mL组、100μg/mL组、150μg/mL组、200μg/mL组、250μg/mL组。CCK-8法、克隆形成实验、划痕愈合实验和Tanswell小室实验、WB法和q PCR法分别检测SK-Hep-1细胞的增殖、迁移、侵袭能力,以及环氧合酶-2(COX-2)、基质金属蛋白酶-2(MMP-2)、MMP-9、组织基质金属蛋白酶抑制剂-1(TIMP-1)、TIMP-2蛋白和MMP-1、MMP-2 mRNA的表达。结果:经质谱鉴定,用Fmoc固相法合成的T-19及RGD-T-19纯度高。T-19和RGD-T-19均能显著抑制SK-Hep-1细胞的增殖、迁移、侵袭能力,抑制COX-2蛋白、MMP-2和MMP-9蛋白及mRNA的表达、促进TIMP-1、TIMP-2蛋白的表达(P <0.05, P <0.01, P <0.001),RGD-T-19的抑制或促进效应均明显强于T-19(均P <0.05)。结论:利用Fmoc固相法合成了纯度高、活性好的T-19及RGD-T-19,两种肽均能抑制SK-Hep-1细胞增殖、侵袭和迁移能力,RGD-T-19作用明显强于T-19。

新疆TASTE台阵拾震器方位角标定及其对SKS波分裂测量的影响

在对塔里木岩石圈地震台阵(TASTE)的66个宽频带流动台站数据进行严格约束基础上,利用3种国际上常用的方法——P波主成分分析法、P波切向最小能量法和Rayleigh波相关系数法,实现地震仪的拾震器方位角标定。3种方法得出的结果具有较高的一致性(相关系数介于0.64~0.91之间),通过综合分析确定最终的地震仪拾震器方位角。其中62个台站地震仪的拾震器方向较稳定,且所有台站均没有出现南北反向、拾震器方位角随时间推移而发生系统性变化的情况。通过与磁偏角对比,发现有40个台站地震仪的拾震器方位角位于磁偏角的±3°范围内,说明TASTE台阵受到的外部干扰较小,台站部署质量较高。通过两个SKS波分裂实例,评估拾震器方位角对SKS波分裂测量产生的影响,论证了在地震学中进行拾震器方位角标定的必要性。

SK扰流器液氮泡沫发生装置混合换热性能研究

为提高液氮泡沫发生装置混合发泡性能、缓解冰堵,探究SK扰流器混合换热特性,开展数值模拟并进行实验验证。通过锥形、SK扰流器对不同液氮流速、间距、形状参数的数值仿真对比分析出口氮气体积分数、变异系数以及沿程温度,并通过因子效应极差分析对比长径比、单元数、间距对混合均匀性的影响效应。研究结果表明:SK扰流器相比于锥形扰流器,通过引入旋流破坏流动分层,变异系数下降83%,气液混合和换热性能大幅提高。此外,随着液氮流量增大,管内局部低于冰点容易形成冰堵,建议最大流量比位于1∶33~1∶50之间。随着液氮射流与扰流器间距增大,液氮汽化率下降,出口氮气体积分数和速度相应降低,10 mm间距效果更优。在300 mm扰流器长度下,随着扰流器长径比减小,混合均匀性先提高后下降,压力损失逐渐增大;综合考虑压力损失和混合均匀度,最优长径比约为0.75;长径比因子对变异系数影响较大。研究结果可为液氮泡沫发生装置设计提供指导,提高泡沫发泡性能。

ZK-2井SK-DLS 2000录井系统应用

ZK-2井是位于临汾盆地西部罗云山断裂东部的一口地质钻探井,实际完钻井深1413.95m,井身结构为小井眼三开结构(Ф153mm×825.43m/Ф139.7mm×825m+Ф122mm×1158.37m/Ф114mm×1158m+Ф98mm×1413.95m),其中二开三开段为绳索取芯,因此,准确进行现场常规钻井参数录测对于现场的施工作业以及各层段油气发现具有十分重要的意义。为此,ZK-2井积极引入了神开公司SK-DLS 2000录井系统,通过对现场钻探施工钻时参数、井口返出泥浆的气测参数、迟到时间参数、钻具组合参数等参数的实时测量,产生了基于ZK-2井施工的大量现场数据,通过现场录井相关参数对比和拟合发现,SK-DLS 2000实时生成的录井数据能指导现场钻井参数优选,能辅助发现现场井漏等钻井复杂,同时SK3Q04色谱仪精度高,基线漂移量小,在地质岩芯钻探录井方面有广阔应用空间。

SK5钢闪光对焊工艺研究与应用

针对SK5钢开展闪光对焊试验,对其焊接接头的显微组织、拉伸性能、显微硬度、弯曲性能进行分析,探索合适的SK5钢闪光对焊焊接生产工艺。结果表明,采用合适的焊接工艺参数,可以获得优质的闪光对焊焊接接头,焊缝区组织由针状和片状马氏体+残余奥氏体组成,热影响区组织由针状和片状马氏体+少量铁素体组成,生成硬脆的片状马氏体组织,组织脆化;焊后进行200℃低温回火处理后焊缝区生成低碳马氏体组织,热影响区生成低碳马氏体和回火马氏体组织,焊接接头组织得到改善,同时其拉伸性能及弯曲性能得到提升。采用合适的闪光焊工艺条件,焊后200℃低温回火处理,能够满足SK5耐磨环焊接应用要求。

SK505-Ⅱ高效气液分离装置试验研究分析

在双碳目标下,针对钢铁行业及燃煤电厂的烟尘、二氧化硫排放提出了“超净排放”、“近零排放”的更高要求。基于此,对SK505-Ⅱ高效气液分离装置进行了改进研究。采用控制变量法对SK505-Ⅱ气液分离装置的两种筒径(φ800、φ500)进行对比分析,得到该高效气液分离装置的最佳流速及对应的阻力范围、气液分离效率及除雾除尘能力等。研究结果为该高效气液分离装置后续应用在不同工况下进一步实现节能减排提供了参考依据和理论支持。

基于UG模型的SK40刀柄预平衡设计

主要讨论了由SK40刀柄不对称结构设计引起的动不平衡问题。通过UG软件建立SK40刀柄预平衡模型,并计算了不平衡量。通过动平衡试验对建模进行验证,试验结果符合理论设计。

Six Amino Acids among Natural Moisturizing Factors Responsible for Skin Hydration: Improvement and Anti-Aging of Skin by Galactomyces Ferment Filtrate-PiteraTM Containing Skin Moisturizer

Background: Natural moisturizing factors (NMFs) are filaggrin-derived components in the cornified layer that are critical for maintaining healthy skin moisturization and barrier function. However, studies have reported conflicting findings on the relationship between NMF levels and aging, while few studies have investigated this relationship clinically. To fill this research gap, we determined the levels of major NMF components such as free amino acids, pyrrolidone carboxylic acid, and urocanic acids, and individually verified their relationships with skin hydration, barrier function, age, and skin aging. Purpose: The objective of this study was to clinically investigate the relationship between NMF components levels and skin aging in facial skin. The main NMF components were obtained from facial skin and quantified. We then selected NMF components showing strong relationships to skin hydration, and analyzed the relationships of the levels of these selected NMF components with signs of skin aging, namely, texture, pores, wrinkles, and dullness (L-value). We also examined the efficacy of treatment with a skin care formula (SK-II Facial Treatment Essence, called SK-II FTE hereafter) including Galactomyces ferment filtrate (GFF, PiteraTM) on the selected NMF component levels associated with skin hydration and barrier function, and the signs of skin aging of texture, pores, wrinkles, and dullness (L-value). Method: We conducted two clinical trials in this research. In Study 1, we measured 23 NMF components using tape-stripped cornified layer to quantify them via an HPLC method in 196 Asian females aged 20 to 59 (mean S.D., 38.6 9.4). Facial visual aging parameters [texture, pores, wrinkles, and dullness (L-value)], as well as elasticity (R7), skin hydration, and TEWL, were quantified using facial skin imaging and skin physical property measurement devices. Study 2 was performed to evaluate whether the facial application of SK-II FTE affects the NMF levels and skin aging parameters in 63 Asian female volunteers aged 20 to 55 (38.4 9.03). During the course of Study 2, 0.6 mL of SK-II FTE was applied to the face twice daily in the morning and afternoon. Skin measurements were performed at the start of the day (baseline) and at week 8. Results: In Study 1, we examined the stratum corneum levels of 23 NMF components comparing to the skin hydration status in 196 female subjects. The subjects were divided into two groups using the median of each measured NMF component. Skin hydration values were compared between the two groups defined for each NMF component. The results showed that subjects with higher levels of six amino acids, alanine, arginine, asparagine, glutamine, glycine, and histidine, exhibited significantly higher skin hydration than those with lower amino acid levels. No significant differences in skin hydration values were found for the other 17 NMF components. We then analyzed whether the sum of these six amino acid NMF components (called 6-AA-NMFs, hereafter) is affected by aging. The 6-AA-NMF level peaked in the subjects aged 25-29, and then gradually and significantly decreased with age. Interestingly, the 6-AA-NMF level was significantly correlated with the skin hydration value, but not with TEWL. In addition, the 6-AA-NMF level demonstrated significant correlations with the signs of skin aging of texture, pores, wrinkles, and dullness (L-value). Then, in Study 2, we examined whether the daily application of SK-II FTE affects the 6-AA-NMF level and visual aging parameters in 63 females. SK-II FTE demonstrated significant increases of the levels of 6-AA-NMFs and each of its components associated with hydration and barrier function, and improvements of skin texture, pores, wrinkles, and dullness (L-value) during the 8 weeks of treatment of facial skin. Conclusion: These clinical studies with large numbers of subjects across a wide age range revealed that six amino acids as NMF components were highly correlated with facial skin hydration in the stratum corneum. The levels of these six NMF components were also found to decrease at ages after the 30 s and were significantly correlated with major signs of skin aging. Notably, these six NMF components (6-AA-NMFs) were increased by SK-II FTE treatment associated with improvements of skin hydration and signs of skin aging, namely, texture, pores, wrinkles, and dullness (L-value). These studies were limited by the lack of investigation of why some NMF components were not associated with skin hydration. More clinical trials examining various NMF components and their relationship with aging are anticipated.

羊栖菜多糖通过miR-7抑制MPP+诱导的SK-N-SH损伤

目的羊栖菜多糖(SFPS)对1-甲基-4-苯基吡啶离子(MPP+)诱导的SK-N-SH损伤的影响及分子机制。方法将SK-N-SH细胞用0.3 mmol·L^(-1)的MPP+处理作为模型(Model)组;以正常培养的细胞作为对照(Con)组,用质量浓度分别为10、30、100 mg·L^(-1)的羊栖菜多糖和0.3 mmol·L^(-1)的MPP+处理作为羊栖菜多糖低、中、高浓度组;将SK-N-SH细胞再分为Model+miR-NC组、Mod-el+miR-7组、Model+SFPS-H+anti-miR-NC组、Model+SFPS-H+anti-miR-7组;实时荧光定量PCR(RT-qPCR)检测miR-7表达水平;流式细胞术检测细胞凋亡;蛋白质印迹(Western blot)法检测蛋白表达;试剂盒检测细胞MDA含量、SOD活性和培养液中LDH含量。结果不同浓度羊栖菜多糖处理后,MPP+诱导的SK-N-SH细胞中miR-7表达水平升高,细胞的凋亡率降低,Bax表达水平降低,Bcl-2表达水平升高,MDA含量和LDH水平降低,SOD活性升高。过表达miR-7后,MPP+诱导的SK-N-SH细胞的凋亡率降低,Bax表达水平降低,Bcl-2表达水平升高,MDA含量和LDH水平降低,SOD活性升高。抑制miR-7逆转了羊栖菜多糖对MPP+诱导的SK-N-SH细胞损伤的作用。结论羊栖菜多糖可能通过上调miR-7抑制MPP+诱导的SK-N-SH细胞损伤。

紫草素通过Keap1/Nrf2信号通路介导的自噬对卵巢癌SK-OV-3细胞的影响

目的探讨紫草素通过Keap1/Nrf2信号通路介导的自噬对卵巢癌SK-OV-3细胞的影响。方法采用MTT法检测细胞增殖情况,计算顺铂、紫草素及在紫草素作用下顺铂的半数抑制浓度(IC50),以确定后续干预浓度。采用pcDNA3-EGFP-C4-Nrf2质粒及其阴性对照对SK-OV-3细胞进行转染,分别记为OE-Nrf2组、NC-Nrf2组。将SK-OV-3细胞分为对照组、顺铂(14μmol·L^(-1))组、顺铂(14μmol·L^(-1))+紫草素(9μmol·L^(-1))组,以及将转染后细胞分为顺铂(14μmol·L^(-1))+紫草素(9μmol·L^(-1))+NC-Nrf2组、顺铂(14μmol·L^(-1))+紫草素(9μmol·L^(-1))+OE-Nrf2组;培养48 h后,采用MTT法检测细胞的增殖抑制情况;RT-qPCR法检测细胞Nrf2mRNA表达情况;Transwell实验检测细胞侵袭能力;AO染色法检测细胞自噬情况;Western Blot法检测细胞增殖、侵袭及自噬相关蛋白(CyclinD1、PCNA、E-cadherin、N-cadherin、Vimentin、Beclin-1、LC3II/I、ULK、Keap1、Nrf2、p62)表达情况。结果(1)与对照组比较,紫草素4~20μmol·L^(-1)浓度组的SK-OV-3细胞活力明显降低(P<0.05),紫草素对SK-OV-3细胞的IC50为9.04μmol·L^(-1);单用顺铂10~80μmol·L^(-1)浓度组及紫草素(9μmol·L^(-1))+顺铂10~80μmol·L^(-1)浓度组的SK-OV-3细胞活力均明显降低(P<0.05)。与单用顺铂组比较,紫草素(9μmol·L^(-1))+顺铂10~80μmol·L^(-1)浓度组的SK-OV-3细胞活力均明显降低(P<0.05)。单用顺铂对SK-OV-3细胞的IC50为28.49μmol·L^(-1);与紫草素(9μmol·L^(-1))联用时,顺铂对SK-OV-3细胞的IC50为14.00μmol·L^(-1)。(2)与NC-Nrf2组比较,OE-Nrf2组SK-OV-3细胞的Nrf2 mRNA表达水平明显升高(P<0.05)。与对照组比较,顺铂组SK-OV-3细胞的Nrf2 mRNA及蛋白表达水平明显升高(P<0.05),Keap1蛋白表达水平明显降低(P<0.05);顺铂组、顺铂+紫草素组SK-OV-3细胞的增殖抑制率明显升高(P<0.05),CyclinD1、PCNA蛋白表达明显下调(P<0.05);单个视野细胞侵袭数目及N-cadherin、Vimentin蛋白表达水平明显降低(P<0.05),E-cadherin蛋白表达水平明显升高(P<0.05);细胞的红色荧光自噬囊泡明显增多,Beclin-1、LC3II/I、ULK1、p62蛋白表达水平明显升高(P<0.05)。与顺铂组比较,顺铂+紫草素组SK-OV-3细胞的Nrf2 mRNA及蛋白表达水平明显降低(P<0.05),Keap1蛋白表达水平明显升高(P<0.05);细胞的增殖抑制率明显升高(P<0.05),CyclinD1、PCNA蛋白表达明显下调(P<0.05);单个视野细胞侵袭数目及N-cadherin、Vimentin蛋白表达水平明显降低(P<0.05),E-cadherin蛋白表达水平明显升高(P<0.05);细胞的红色荧光自噬囊泡减少,Beclin-1、LC3II/I、ULK1、p62蛋白表达水平明显降低(P<0.05)。与顺铂+紫草素+NC-Nrf2组比较,顺铂+紫草素+OE-Nrf2组SK-OV-3细胞的增殖抑制率明显降低(P<0.05),CyclinD1、PCNA蛋白表达明显上调(P<0.05);单个视野细胞侵袭数目及N-cadherin、Vimentin蛋白表达水平明显升高(P<0.05),E-cadherin蛋白表达水平明显降低(P<0.05);细胞的红色荧光自噬囊泡增多,Beclin-1、LC3II/I、ULK1、p62蛋白表达水平明显升高(P<0.05)。结论紫草素可降低顺铂对卵巢癌SK-OV-3细胞的IC50,提高其对顺铂的敏感性,并抑制其增殖、侵袭能力,可能与其抑制Keap1/Nrf2通路介导的自噬有关。

FHL2通过SK-1/S1P通路改善高糖诱导内皮细胞功能障碍的机制研究

目的探讨四个半LIM结构域蛋白2(FHL2)对高糖引起内皮细胞功能障碍的影响及其作用机制。方法采用MTT检测含不同浓度葡萄糖培养基培养下的人脐静脉内皮细胞(HUVECs)的细胞活力。将HUVECs分为对照组(5 mmol/L葡萄糖)、高糖组(30 mmol/L葡萄糖)、siNC组(30 mmol/L葡萄糖+siNC)和siFHL2组(30 mmol/L葡萄糖+siFHL2)。利用Western blot检测对照组和高糖组的HUVECs中FHL2蛋白表达水平。采用实时定量PCR法检测转染siFHL2后FHL2的表达水平。运用Annexin V-FITC/PI双染检测各组细胞凋亡情况。利用Western blot检测凋亡相关蛋白FHL2、B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、cleaved caspase 3表达水平。采用成管实验检测各组HUVECs的成管能力。利用Western blot检测各组SK-1/1-磷酸鞘氨醇(SK-1/S1P)通路中的蛋白表达水平,酶联免疫吸附(ELISA)检测S1P表达水平变化。结果与对照组相比,高糖组HUVECs内FHL2表达上调(t=4.407,P<0.05)。与对照组相比,高糖组HUVECs凋亡水平升高,Bax和cleaved caspase 3的表达也较对照组显著上调,而下调FHL2表达后,细胞凋亡减少,凋亡相关蛋白Bax和cleaved caspase 3的表达下调(P<0.05)。此外,FHL2表达下调改善了HUVECs的成管能力。分子机制分析显示,与对照组比较,高糖组p-SK-1表达显著下调(P<0.05),而下调FHL2后p-SK-1水平显著上调(P<0.05),S1P水平也显著上调(P<0.05)。结论FHL2可改善高糖引起的内皮细胞功能障碍,其作用机制可能与SK-1/S1P信号通路有关。

陆地棉SKS基因家族全基因组特征分析与GhSKS13抗黄萎病功能解析

鉴定陆地棉SKS基因家族成员,解析其演化规律,为棉花抗性育种提供新的候选基因。基于已公布的陆地棉遗传标准系TM-1基因组数据,以陆地棉品种中棉-14为试验材料。通过生物信息学对陆地棉SKS家族成员进行全基因组鉴定,并对其家族成员的染色体分布、进化关系、基因结构、共线性关系等进行预测分析。通过实时荧光定量聚合酶链式反应分析GhSKS13的表达模式,并利用病毒诱导的基因沉默技术初步探究陆地棉SKS13在棉花抵御大丽轮枝菌过程中的功能。共鉴定到48个陆地棉SKS基因,不均匀分布于陆地棉19条染色体上,聚类分析分为5个亚组,基因序列具有较高保守性,共线性关系分析显示,陆地棉SKS基因家族受到纯化选择。组织模式表达分析显示,GhSKS13在陆地棉根组织中优势表达,并且受大丽轮枝菌诱导显著上调表达。GhSKS13沉默植株对大丽轮枝菌抗性减弱,同时抑制病程相关基因GhPR1、GhPR2、GhPR3和GhPR5表达。大丽轮枝菌入侵GhSKS13沉默植株,其过氧化氢(H 2 O 2)沉积明显低于对照,暗示GhSKS13促进活性氧(ROS)的形成。总之,明确了陆地棉SKS家族成员的系统进化关系、染色体分布特征和基因结构特征;并阐明了GhSKS13参与棉花对大丽轮枝菌抗性反应。

基于BSRes_SK_GRU的雷达信号调制样式识别

针对基于图像识别的雷达信号调制样式识别方法生成图像耗时长,收敛速度慢,且在低信噪比条件下识别准确率低的问题,提出了一种新的雷达信号调制样式识别模型。此模型将雷达信号时间序列经简单预处理后直接作为网络输入,避免了将信号转换为图像的复杂过程;网络能够自主提取雷达信号空间和时间两个方面特征,完善了特征提取的方式;同时,对网络结构进行了优化,并引入了选择性核网络,以获取特征在不同尺度上的重要信息。实验结果表明,此模型在低信噪比条件下具有较快的训练速度和较高的识别准确率。

SK演绎“幸福经营”的ESG故事

韩国三大跨国企业集团之一SK集团(简称SK)倡导企业积极主动创造社会价值,摆脱被动履行社会责任,通过创建并实践ESG经营核心指标体系、构建碳减排九大生态系统、向碳中和业务转型和治理模式变革,持续演绎“幸福经营”的ESG故事,为利益相关方的幸福和社会可持续发展做出贡献。

生科SK-800全自动血型分析仪的输血相容性检测性能评价

目的 评价使用生科SK-800全自动血型分析仪进行ABO/Rh(D)血型鉴定、交叉配血和不规则抗体筛查检测的性能。方法 留取室间质评样本和临床样本,从准确度、稳定性、抗体检出限及干扰试验4个方面进行综合评价。结果 生科SK-800全自动血型分析仪在ABO/Rh(D)血型鉴定、交叉配血和不规则抗体筛查等项目的一致率及重复率均≥80%;抗体效价检测的灵敏度优于手工试管法;轻度溶血、脂血及黄疸样本对血型鉴定结果无干扰。结论 生科SK-800全自动血型分析仪检测性能良好,具备无携带污染和检测速度快等优势,可有效提高工作效率,具有较高的临床应用价值。

吴茱萸碱对神经母细胞瘤SK-N-SH细胞增殖、迁移及侵袭的影响

目的:探讨吴茱萸碱(Evo)是否通过调控lncRNA LINC00858表达调控神经母细胞瘤SK-N-SH细胞的增殖、迁移及侵袭。方法:在体外以3、6、12μmol/L Evo处理人神经母细胞瘤SK-N-SH细胞,利用RNA干扰技术分别将si-NC、si-LINC00858转染至SK-N-SH细胞,将pcDNA、pcDNA-LINC00858转染至SK-N-SH细胞并经12μmol/L Evo处理,实验分为对照组、Evo低剂量组、Evo中剂量组、Evo高剂量组、si-NC组、si-LINC00858组、Evo+pcDNA组、Evo+pcDNA-LINC00858组。采用q PCR法检测各组细胞LINC00858的表达量,MTT、Transwell实验分别检测细胞的增殖、迁移、侵袭能力,WB法检测细胞中cyclinD1、MMP-2、MMP-9和p21蛋白的表达。结果:与对照组相比,Evo低、中、高剂量组SK-N-SH细胞中LINC00858表达均显著降低(均P<0.05),细胞增殖抑制率显著升高、迁移及侵袭细胞数显著减少(均P<0.01),cyclinD1、MMP-2、MMP-9蛋白表达降低、p21蛋白表达升高(均P<0.01)。与si-NC组相比,si-LINC00858组细胞的增殖抑制率、迁移和侵袭细胞数及相关蛋白表达变化同Evo低、中、高剂量组。与Evo+pcDNA组相比,Evo+pcDNA-LINC00858组细胞的增殖抑制率显著降低、迁移及侵袭细胞数均显著增多(均P<0.01),cyclinD1、MMP-2、MMP-9蛋白表达升高、p21蛋白表达降低(均P<0.05)。结论:Evo通过下调LINC00858表达抑制神经母细胞瘤SK-N-SH细胞的增殖、迁移及侵袭。

胃癌临床诊断中血清外泌体长链非编码RNA?RN7SK的作用分析

围绕经确诊的胃癌患者,对其血清外泌体长链非编码RNA RN7SK(lnc RNA RN7SK)进行检测,评定其在诊断此病中的价值。方法 别对原发胃癌患者、健康对照者各10例进行选取,收集其血清,对外泌体展开相应提取。采用定量逆转录聚合酶链式反应(qRT-PCR)法对lnc RNA RN7SK的表达实施检测,深入剖析其与胃癌病理学特征之间所存在的关系;用电化学发光法对癌胚抗原(CEA)水平进行测定,进行ROC曲线的绘制,探讨其实际诊断价值。结果 针对血清外泌体lnc RNA RN7SK,其在胃癌患者当中的表达,与健康对照者相比,明显偏高(P<0.05)。血清外泌体lnc RNA RN7SK的表达水平密切相关于胃癌患者的淋巴结转移、肿瘤直径、TNM分期、分化程度、远处转移。外泌体lnc RNA RN7SK相对应的ROC曲线下面积(AUCROC)是0.830,其在具体的检验效能上,较CEA(0.579)优。将两者进行联合的AUCROC是0.853,相比单纯CEA、lnc RNA RN7SK,明显偏优。结论 针对胃癌患者,在其血清外泌体当中,lnc RNA RN7SK存在高表达情况,且与TNM 分期、淋巴结转移、肿瘤直径、远处转移、浸润深度之间存在紧密的相关性,诊断效能突出,可当作诊断此病的潜在分子标志物。

团结经济视角下乡村亲子游服务探索--以SK村为例

为了进一步探索寓教于乐、促进孩子健康成长的亲子游模式,通过个案研究法,以SK村为例反思当前亲子旅游市场存在的活动形式单一、商业化过浓、缺乏教育性等问题,梳理出城乡互为主体的团结经济视角下新型乡村亲子游模式,总结出“因地创造—共创生产—公平消费—民主管理—盈余分配”的循环实务流程。

乙酰胆碱及其拮抗剂对人SK-N-SH细胞增殖及mAchR1表达的影响

用CCK-8比色法和流式细胞术,检测乙酰胆碱(acetylcholine,Ach)及拮抗剂阿托品(atropine,Atro)对SK-N-SH细胞增殖活性和周期分布的调节作用;进一步用荧光定量PCR、免疫印迹和流式细胞间接免疫荧光技术,分析SK-N-SH细胞毒蕈碱受体亚型型(mAchR1)和c-fos的表达差异。结果表明,1mmol/L Ach对SK-N-SH细胞有明显促增殖作用,而1mmol/L Atro阻滞细胞从S期向G2/M期移行;1mmol/L Ach与1mmol/L Atro均反馈调节mAchR1的蛋白水平,但mAchR1mRNA的表达不受影响;1mmol/L Ach显著上调c-fos mRNA和Fos蛋白的表达,但这种作用可被Atro逆转。提示胆碱类受体参与配基对肿瘤细胞的促增殖作用。