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Rho/ROCK信号通路在糖尿病视网膜病变中的研究进展
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作者 唐云华 刘春梦 +2 位作者 邢尧 柯殷雨 张富文 《中医眼耳鼻喉杂志》 2024年第3期152-155,共4页
糖尿病视网膜病变(diabetic retinopathy,DR)是导致劳动年龄人群视力障碍的重要原因,其复杂的发病机制尚未完全明确。虽然一些新兴治疗方法在DR的治疗中取得显著疗效,但仍存在治疗效果欠佳及并发症等问题。Rho GTP酶是一种小分子蛋白,... 糖尿病视网膜病变(diabetic retinopathy,DR)是导致劳动年龄人群视力障碍的重要原因,其复杂的发病机制尚未完全明确。虽然一些新兴治疗方法在DR的治疗中取得显著疗效,但仍存在治疗效果欠佳及并发症等问题。Rho GTP酶是一种小分子蛋白,通过激活下游蛋白ROCK参与细胞骨架调节等多种细胞活动。在DR发病过程中Rho/ROCK信号通路被激活,抑制Rho/ROCK信号传导可能抑制DR的进展。本文从炎症、新生血管、血-视网膜屏障(blood retinal barrier,BRB)、晚期糖基化终末产物(advanced glycation end products,AGEs)、糖尿病视网膜神经变性(diabetic retinal neurodegeneration,DRN)等方面阐述Rho/ROCK信号通路在DR发病机制中的研究进展,以期为DR的治疗提供新思路。 展开更多
关键词 糖尿病视网膜病变 Rho GTP酶 rock RHO/rock信号通路 rock抑制剂
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Disturbance failure mechanism of highly stressed rock in deep excavation:Current status and prospects
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作者 Tao Wang Weiwei Ye +3 位作者 Liyuan Liu Kai Liu Naisheng Jiang Xianhui Feng 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第4期611-627,共17页
This article reviews the current status on the dynamic behavior of highly stressed rocks under disturbances.Firstly,the experimental apparatus,methods,and theories related to the disturbance dynamics of deep,high-stre... This article reviews the current status on the dynamic behavior of highly stressed rocks under disturbances.Firstly,the experimental apparatus,methods,and theories related to the disturbance dynamics of deep,high-stress rock are reviewed,followed by the introduction of scholars’research on deep rock deformation and failure from an energy perspective.Subsequently,with a backdrop of highstress phenomena in deep hard rock,such as rock bursts and core disking,we delve into the current state of research on rock microstructure analysis and residual stresses from the perspective of studying the energy storage mechanisms in rocks.Thereafter,the current state of research on the mechanical response and the energy dissipation of highly stressed rock formations is briefly retrospected.Finally,the insufficient aspects in the current research on the disturbance and failure mechanisms in deep,highly stressed rock formations are summarized,and prospects for future research are provided.This work provides new avenues for the research on the mechanical response and damage-fracture mechanisms of rocks under high-stress conditions. 展开更多
关键词 deep rock with high stress highly stressed rock rock failure residual stress energy release
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Coupled DEM-FDM analyses of the effects of falling rock’s shape and impact angle on response of granular cushion and rock shed
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作者 Jingyu Xue Chen Cao +2 位作者 Jianhua Yan Yaopeng Ji Jianping Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第8期3353-3364,共12页
Rock shed is an effective protection measure against rockfall.To investigate the influences of falling rock’s shape and impact angle on the impact effect of the cushioned rock shed,a modeling approach for a rock shed... Rock shed is an effective protection measure against rockfall.To investigate the influences of falling rock’s shape and impact angle on the impact effect of the cushioned rock shed,a modeling approach for a rock shed with a cushion layer using PFC-FLAC.The granular cushion is modeled as an aggregate of discrete non-cohesion particles,while the concrete plate and the beam are modeled as zones.The falling rock with different sphericities and impact angles is modeled as a rigid assembly.The numerical model is validated by comparing the simulation results with experimental and numerical results from previous literature.This model is applied to analyze the effects of rock shape and impact angle on the dynamic interaction effects between falling rock and cushioned rock shed,including the impact force,transmitted bottom force,penetration depth,and plate deflection.The numerical results show that the variation in the falling rock’s shape has different effects on the falling rock with different impact angles.These findings could support rock shed design by revealing the limitations of the assumptions in the past research,which may result in unsafe rock sheds for some rockfall cases. 展开更多
关键词 rockfall Coupled DEM-FDM analysis rock’s shape Impact angle Granular cushion rock shed
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Numerical modeling of blast-induced rock fragmentation in deep mining with 3D and 2D FEM method approaches
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作者 MichałKucewicz MazurkiewiczŁukasz +4 位作者 PawełBaranowski Jerzy Małachowski Krzysztof Fuławka Piotr Mertuszka Marcin Szumny 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第11期4532-4553,共22页
To optimize the excavation of rock using underground blasting techniques,a reliable and simplified approach for modeling rock fragmentation is desired.This paper presents a multistep experimentalnumerical methodology ... To optimize the excavation of rock using underground blasting techniques,a reliable and simplified approach for modeling rock fragmentation is desired.This paper presents a multistep experimentalnumerical methodology for simplifying the three-dimensional(3D)to two-dimensional(2D)quasiplane-strain problem and reducing computational costs by more than 100-fold.First,in situ tests were conducted involving single-hole and free-face blasting of a dolomite rock mass in a 1050-m-deep mine.The results were validated by laser scanning.The craters were then compared with four analytical models to calculate the radius of the crushing zone.Next,a full 3D model for single-hole blasting was prepared and validated by simulating the crack length and the radius of the crushing zone.Based on the stable crack propagation zones observed in the 3D model and experiments,a 2D model was prepared.The properties of the high explosive(HE)were slightly reduced to match the shape and number of radial cracks and crushing zone radius between the 3D and 2D models.The final methodology was used to reproduce various cut-hole blasting scenarios and observe the effects of residual cracks in the rock mass on further fragmentation.The presence of preexisting cracks was found to be crucial for fragmentation,particularly when the borehole was situated near a free rock face.Finally,an optimization study was performed to determine the possibility of losing rock continuity at different positions within the well in relation to the free rock face. 展开更多
关键词 Dolomite rock Two-dimensional(2D)rock modeling rock fragmentation Cut-hole blasting
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SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能 被引量:1
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作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p rock1
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Impact resistance performance and optimization of the sand-EPE composite cushion in rock sheds 被引量:2
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作者 YU Bingxin ZHOU Xiaojun +2 位作者 TANG Jianhui ZHANG Yujin ZHANG Yuefeng 《Journal of Mountain Science》 SCIE CSCD 2024年第2期676-689,共14页
Rock sheds are widely used to prevent rockfall disasters along roads in mountainous areas.To improve the capacity of rock sheds for resisting rockfall impact,a sand and expandable polyethylene(EPE)composite cushion wa... Rock sheds are widely used to prevent rockfall disasters along roads in mountainous areas.To improve the capacity of rock sheds for resisting rockfall impact,a sand and expandable polyethylene(EPE)composite cushion was proposed.A series of model experiments of rockfall impact on rock sheds were conducted,and the buried depth of the EPE foam board in the sand layer was considered.The impact load and dynamic response of the rock shed were investigated.The results show that the maximum impact load and dynamic response of the rock shed roof are all significantly less than those of the sand cushion.Moreover,as the distance between the EPE foam board and rock shed roof decreases,the maximum rockfall impact force and impact pressure gradually decrease,and the maximum displacement,acceleration and strain of the rock shed first decrease and then change little.In addition,the vibration acceleration and vertical displacement of the rock shed roof decrease from the centre to the edge and decrease faster along the longitudinal direction than that along the transverse direction.In conclusion,the buffering effect of the sand-EPE composite cushion is better than that of the pure sand cushion,and the EPE foam board at a depth of 1/3 the thickness of the sand layer is appropriate. 展开更多
关键词 rockFALL rock shed Impact Composite cushion Buffering effect Dynamic response
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贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响
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作者 彭博文 冯立 覃剑 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第7期980-984,共5页
目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组... 目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组、贝母素乙(3.5 mg/kg)+LPA(RhoA激活剂,1 mg/kg)组,每组10只,另选10只大鼠为对照组。机械刺激法和热辐射法检测大鼠膝关节疼痛症状;检测大鼠关节肿胀度、膝关节宽度并以步态评分评测其关节功能;透射电镜检测大鼠膝关节软骨细胞超微结构;TUNEL染色检测大鼠关节软骨细胞凋亡比;酶联免疫吸附(ELISA)法检测大鼠血清和关节液促炎因子白细胞介素(IL)-18、单核细胞趋化蛋白1(MCP-1)水平;免疫印迹检测各组大鼠关节软骨组织凋亡(Cleaved caspase-3、Bax)和RhoA/ROCK通路相关蛋白表达。结果 贝母素乙可降低KOA大鼠关节肿胀度、膝关节宽度、步态评分、软骨细胞凋亡比、血清及关节液IL-18、MCP-1水平、软骨组织Cleaved caspase-3、Bax、RhoA、ROCK1、ROCK1蛋白表达,升高机械痛阈值、热痛阈值(P<0.05);LPA可减弱贝母素乙对KOA大鼠关节软骨损伤的改善作用。结论 贝母素乙可通过抑制RhoA/ROCK信号激活而抑制KOA大鼠关节炎症反应和关节软骨细胞凋亡,减轻软骨损伤。 展开更多
关键词 贝母素乙 RhoA/rock 膝骨关节炎 软骨损伤 信号通路
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桃叶珊瑚苷调节RhoA/ROCK信号通路对胶质母细胞瘤细胞活力和上皮间质转化的影响
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作者 李娟 石海平 李维民 《天津中医药》 CAS 2024年第3期386-392,共7页
[目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(C... [目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(CCK-8)法检测细胞活力,流式细胞术检测细胞凋亡,Transwell小室实验检测细胞迁移和侵袭,蛋白免疫印迹法(Western Blot)检测基质金属蛋白酶(MMP)2、MMP9、波形蛋白(Vimentin)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。构建GBM裸鼠模型,随机分为裸鼠对照组、AU组、Y-27632组、AU+Y-27632组,测量肿瘤质量与体积,免疫组化法检测移植瘤组织RhoA、ROCK1、ROCK2蛋白表达。[结果]GBM细胞活力随着AU浓度的升高而逐渐降低(P<0.05),选择U87作为后续实验细胞,选择10、25、50μmol/L浓度作为AU后续实验浓度。与对照组比较,AU低、中、高浓度组和Y-27632组细胞活力、迁移和侵袭细胞数、MMP2、MMP9、N-cadherin、Vimentin、RhoA、ROCK1、ROCK2表达显著下降,凋亡率、E-cadherin表达显著升高(P<0.05),其中高浓度AU和Y-27632组共同处理的细胞变化更显著(P<0.05)。AU和Y-27632均能抑制移植瘤质量和体积,降低RhoA/ROCK信号通路蛋白表达(P<0.05)。[结论]AU能抑制GBM细胞活力、迁移侵袭和EMT,促进细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 胶质母细胞瘤 细胞活力 上皮间质转化 桃叶珊瑚苷 RhoA/rock信号通路
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Discontinuity development patterns and the challenges for 3D discrete fracture network modeling on complicated exposed rock surfaces 被引量:1
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作者 Wen Zhang Ming Wei +8 位作者 Ying Zhang Tengyue Li Qing Wang Chen Cao Chun Zhu Zhengwei Li Zhenbang Nie Shuonan Wang Han Yin 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2154-2171,共18页
Natural slopes usually display complicated exposed rock surfaces that are characterized by complex and substantial terrain undulation and ubiquitous undesirable phenomena such as vegetation cover and rockfalls.This st... Natural slopes usually display complicated exposed rock surfaces that are characterized by complex and substantial terrain undulation and ubiquitous undesirable phenomena such as vegetation cover and rockfalls.This study presents a systematic outcrop research of fracture pattern variations in a complicated rock slope,and the qualitative and quantitative study of the complex phenomena impact on threedimensional(3D)discrete fracture network(DFN)modeling.As the studies of the outcrop fracture pattern have been so far focused on local variations,thus,we put forward a statistical analysis of global variations.The entire outcrop is partitioned into several subzones,and the subzone-scale variability of fracture geometric properties is analyzed(including the orientation,the density,and the trace length).The results reveal significant variations in fracture characteristics(such as the concentrative degree,the average orientation,the density,and the trace length)among different subzones.Moreover,the density of fracture sets,which is approximately parallel to the slope surface,exhibits a notably higher value compared to other fracture sets across all subzones.To improve the accuracy of the DFN modeling,the effects of three common phenomena resulting from vegetation and rockfalls are qualitatively analyzed and the corresponding quantitative data processing solutions are proposed.Subsequently,the 3D fracture geometric parameters are determined for different areas of the high-steep rock slope in terms of the subzone dimensions.The results show significant variations in the same set of 3D fracture parameters across different regions with density differing by up to tenfold and mean trace length exhibiting differences of 3e4 times.The study results present precise geological structural information,improve modeling accuracy,and provide practical solutions for addressing complex outcrop issues. 展开更多
关键词 Complicated exposed rock surfaces Discontinuity characteristic variation Three-dimensional discrete fracture network modeling Outcrop study Vegetation cover and rockfalls
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川芎嗪抑制ROCK的表达降低模拟失重大鼠血管Ca^(2+)敏感性
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作者 王慧平 白晓镯 +4 位作者 赵晶 赵省心 刘朕印 党凯 高云芳 《西北大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第5期919-928,共10页
研究失重条件下血管平滑肌收缩性、Ca^(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)... 研究失重条件下血管平滑肌收缩性、Ca^(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)或KCl诱发的血管收缩性和Ca^(2+)敏感性增强,RhoA激酶2(ROCK II)的表达、肌球蛋白磷酸酶靶亚基1(MYPT1)和肌球蛋白调节轻链(MLC)的磷酸化水平均上升,血管孵育Y-27632(ROCK特异性抑制剂)后可降低以上变化。模拟失重大鼠灌饲川芎嗪亦可降低以上变化。模拟失重后,大鼠肠系膜上动脉的收缩性和Ca^(2+)敏感性、ROCK II的表达、MYPT1和MLC的磷酸化水平降低,血管孵育Y-27632对以上变化无明显作用。模拟失重大鼠灌饲川芎嗪亦对以上变化无明显作用。结果表明,由RhoA-ROCK调控的血管平滑肌Ca^(2+)敏感性的变化可能是失重条件下机体前后部血管收缩性发生区域性重塑的关键因素。川芎嗪可抑制ROCK蛋白的表达,降低血管平滑肌升高的Ca^(2+)敏感性,从而纠正失重条件下机体前部血管收缩性的增强,但对失重条件下机体后部血管收缩性的减弱无恢复作用。 展开更多
关键词 Ca^(2+)敏感性 RhoA-rock 血管收缩性 模拟失重 川芎嗪
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Influence of Magmatic Intrusion on Abnormal Hydrocarbon Generation and Expulsion of Source Rock:A Case Study of the Dongying Sag,Bohai Bay Basin
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作者 LI Chuanming ZENG Jianhui +2 位作者 WANG Maoyun LONG Hui LIU Shuning 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2024年第5期1322-1337,共16页
How gabbro affects the generation and expulsion of hydrocarbons in muddy surrounding rocks is clarified by analyzing thin section,major and trace elements,total organic carbon(TOC),pyrolysis,extracts and vitrinite ref... How gabbro affects the generation and expulsion of hydrocarbons in muddy surrounding rocks is clarified by analyzing thin section,major and trace elements,total organic carbon(TOC),pyrolysis,extracts and vitrinite reflectance data from source rocks in the Chunxi area the Dongying Sag,Bohai Bay Basin,eastern China.The results show that a magma intrusion brings copious heat to the source rocks,which promotes abnormal maturation of organic matter(OM)and rapid hydrocarbon generation.The CH_(4)and H_(2)produced by gabbro alteration play a role in hydrocarbon generation of source rocks.The hydrothermal process during magma intrusion provides many different minerals to the source rock,resulting in carbonate-rich surrounding mudstone.The carbonate and clay minerals produced by volcanic mineral alteration jointly catalyze the hydrocarbon generation of the source rock.The high-temperature baking of the intrusion results in hydrothermal pressurization and hydrocarbon generation pressurization,causing many fractures in the surrounding rock.The generated oil and gas are discharged through the fractures under diffusion and pressure.Mantle-derived CO_(2)is also conducive to the expulsion of hydrocarbons because of its strong enrichment capacity for hydrocarbons. 展开更多
关键词 igneous rock hydrocarbon generation source rock high temperature CATALYSIS EOCENE Dongying Sag
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A review of rock macro-indentation:Theories,experiments,simulations,and applications
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作者 Weiqiang Xie Xiaoli Liu +2 位作者 Xiaoping Zhang Xinmei Yang Xiaoxiong Zhou 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2351-2374,共24页
Rock macro-indentation plays a fundamental role in mechanical rock breaking for various rock engineering application,such as drilling,tunneling,cutting,and sawing.Over the past decades,extensive research has been cond... Rock macro-indentation plays a fundamental role in mechanical rock breaking for various rock engineering application,such as drilling,tunneling,cutting,and sawing.Over the past decades,extensive research has been conducted to understand the indentation mechanisms and responses through various approaches.This review aims to provide an overview of the current status and recent advancements in theories,experiments,numerical simulations,and applications of macro-indentation in rock engineering.It starts with elaborating on the mechanisms of macro-indentation,followed by a discussion of the merits and limitations of commonly used models.Influence factors and their effects on indentation test results are then summarized.Various numerical simulation methods for rock macro-indentation are highlighted,along with their advantages and disadvantages.Subsequently,the applications of indentation tests and indentation indices in characterizing rock properties are explored.It reveals that compression-tension,compression-shear,and composite models are widely employed in rock macroindentation.While the compression-tension model is straightforward to use,it may overlook the anisotropic properties of rocks.On the other hand,the composite model provides a more comprehensive description of rock indentation but requires complex calculations.Additionally,factors,such as indentation rate,indenter geometry,rock type,specimen size,and confining pressure,can significantly influence the indentation results.Simulation methods for macro-indentation encompass continuous medium,discontinuous medium,and continuous-discontinuous medium methods,with selection based on their differences in principle.Furthermore,rock macro-indentation can be practically applied to mining engineering,tunneling engineering,and petroleum drilling engineering.Indentation indices serve as valuable tools for characterizing rock strength,brittleness,and drillability.This review sheds light on the development of rock macro-indentation and its extensive application in engineering practice.Specialists in the field can gain a comprehensive understanding of the indentation process and its potential in various rock engineering endeavors. 展开更多
关键词 rock macro-indentation Indentation test Indentation indices MECHANISM rock breaking
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A statistical damage-based constitutive model for shearing of rock joints in brittle drop mode
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作者 Xinrong Liu Peiyao Li +5 位作者 Xueyan Guo Xinyang Luo Xiaohan Zhou Luli Miao Fuchuan Zhou Hao Wang 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第8期1041-1058,共18页
Some rock joints exhibit significant brittleness,characterized by a sharp decrease in shear stress upon reaching the peak strength.However,existing models often fail to accurately represent this behavior and are encum... Some rock joints exhibit significant brittleness,characterized by a sharp decrease in shear stress upon reaching the peak strength.However,existing models often fail to accurately represent this behavior and are encumbered by numerous parameters lacking clear mechanical significance.This study presents a new statistical damage constitutive model rooted in both damage mechanics and statistics,containing only three model parameters.The proposed model encompasses all stages of joint shearing,including the compaction stage,linear stage,plastic yielding stage,drop stage,strain softening stage,and residual strength stage.To derive the analytical expression of the constitutive model,three boundary conditions are introduced.Experimental data from both natural and artificial rock joints is utilized to validate the model,resulting in average absolute relative errors ranging from 3%to 8%.Moreover,a comparative analysis with established models illustrates that the proposed model captures stress drop and post-peak strain softening more effectively,with model parameters possessing clearer mechanical interpretations.Furthermore,parameter analysis is conducted to investigate the impacts of model parameters on the curves and unveil the relationship between these parameters and the mechanical properties of rock joints.Importantly,the proposed model is straightforward in form,and all model parameters can be obtained from direct shear tests,thus facilitating the utilization in numerical simulations. 展开更多
关键词 rock joints Brittle rock Direct shear test Damage-based constitutive model Parameters analysis
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Predicting the probability distribution of Martian rocks mechanical property based on microscale rock mechanical experiments and accurate grain-based modeling
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作者 Shuohui Yin Yingjie Wang Jingang Liu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第9期1327-1339,共13页
The exploration of Mars would heavily rely on Martian rocks mechanics and engineering technology.As the mechanical property of Martian rocks is uncertain,it is of utmost importance to predict the probability distribut... The exploration of Mars would heavily rely on Martian rocks mechanics and engineering technology.As the mechanical property of Martian rocks is uncertain,it is of utmost importance to predict the probability distribution of Martian rocks mechanical property for the success of Mars exploration.In this paper,a fast and accurate probability distribution method for predicting the macroscale elastic modulus of Martian rocks was proposed by integrating the microscale rock mechanical experiments(micro-RME),accurate grain-based modeling(AGBM)and upscaling methods based on reliability principles.Firstly,the microstructure of NWA12564 Martian sample and elastic modulus of each mineral were obtained by micro-RME with TESCAN integrated mineral analyzer(TIMA)and nanoindentation.The best probability distribution function of the minerals was determined by Kolmogorov-Smirnov(K-S)test.Secondly,based on best distribution function of each mineral,the Monte Carlo simulations(MCS)and upscaling methods were implemented to obtain the probability distribution of upscaled elastic modulus.Thirdly,the correlation between the upscaled elastic modulus and macroscale elastic modulus obtained by AGBM was established.The accurate probability distribution of the macroscale elastic modulus was obtained by this correlation relationship.The proposed method can predict the probability distribution of Martian rocks mechanical property with any size and shape samples. 展开更多
关键词 Probability distribution Martian rocks Microscale rock mechanic experiment Nanoindentation Accurate grain-based modeling
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Theory,technology and application of grouted bolting in soft rock roadways of deep coal mines
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作者 Hongpu Kang Jianwei Yang +4 位作者 Pengfei Jiang Fuqiang Gao Wenzhou Li Jiafeng Li Huiyuan Chen 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第7期1463-1479,共17页
The grouted bolt,combining rock bolting with grouting techniques,provides an effective solution for controlling the surrounding rock in deep soft rock and fractured roadways.It has been extensively applied in numerous... The grouted bolt,combining rock bolting with grouting techniques,provides an effective solution for controlling the surrounding rock in deep soft rock and fractured roadways.It has been extensively applied in numerous deep mining areas characterized by soft rock roadways,where it has demonstrated remarkable control results.This article systematically explores the evolution of grouted bolting,covering its theoretical foundations,design methods,materials,construction processes,monitoring measures,and methods for assessing its effectiveness.The overview encompassed several key elements,delving into anchoring theory and grouting reinforcement theory.The new principle of high pretensioned high-pressure splitting grouted bolting collaborative active control is introduced.A fresh method for dynamic information design is also highlighted.The discussion touches on both conventional grouting rock bolts and cable bolts,as well as innovative grouted rock bolts and cables characterized by their high pretension,strength,and sealing hole pressure.An examination of the merits and demerits of standard inorganic and organic grouting materials versus the new inorganic–organic composite materials,including their specific application conditions,was conducted.Additionally,the article presents various methods and instruments to assess the support effect of grouting rock bolts,cable bolts,and grouting reinforcement.Furthermore,it provides a foundation for understanding the factors influencing decisions on grouted bolting timing,the sequence of grouting,the pressure applied,the volume of grout used,and the strategic arrangement of grouted rock bolts and cable bolts.The application of the high pretensioned high-pressure splitting grouted bolting collaborative control technology in a typical kilometer-deep soft rock mine in China—the soft coal seam and soft rock roadway in the Kouzidong coal mine,Huainan coal mining area,was introduced.Finally,the existing problems in grouted bolting control technology for deep soft rock roadways are analyzed,and the future development trend of grouted bolting control technology is anticipated. 展开更多
关键词 deep coal mine soft rock roadway grouted bolting rock bolt and cable grouting material high-pressure splitting grouting collaborative control technology
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Rock strength weakening subject to principal stress rotation:Experimental and numerical investigations
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作者 Huandui Liu Guibin Wang +2 位作者 Chunhe Yang Junyue Zhang Shiwan Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第9期3544-3557,共14页
During the construction and operation of gas storage reservoirs,changes in the principal stress direction can induce fracture propagation under conditions of lower differential stress,potentially leading to failure in... During the construction and operation of gas storage reservoirs,changes in the principal stress direction can induce fracture propagation under conditions of lower differential stress,potentially leading to failure in the surrounding rock.However,the weakening of strength due to pure stress rotation has not yet been investigated.Based on fracture mechanics,an enhanced Mohr-Coulomb strength criterion considering stress rotation is proposed and verified with experimental and numerical simulations.The micro-damage state and the evolution of the rock under the pure stress-rotation condition are analyzed.The findings indicate that differential stress exceeding the crack initiation stress is a prerequisite for stress rotation to promote the development of rock damage.As the differential stress increases,stress rotation is more likely to induce rock damage,leading to a transition from brittle to plastic failure,characterized by wider fractures and a more complex fracture network.Overall,a negative exponential relationship exists between the stress rotation angle required for rock failure and the differential stress.The feasibility of applying the enhanced criterion to practical engineering is discussed using monitoring data obtained from a mine-by tunnel.This study introduces new concepts for understanding the damage evolution of the surrounding rock under complex stress paths and offers a new theoretical basis for predicting the damage of gas storage reservoirs. 展开更多
关键词 Principal stress rotation(PSR) Fracture mechanics Hollow cylinder torsional apparatus for rock (HCAR) Particle flow method rock strength
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Resilient performance of self-centering hybrid rocking walls with curved interface under pseudo-static loading
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作者 Su Xing Yan Shi +1 位作者 Sun Xianglei Wang Tao 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第1期65-85,共21页
Frame and rocking wall(FRW)structures have excellent resilient performance during earthquakes.However,the concrete at interfacial corners of rocking walls(RWs)is easily crushed due to local extreme compression during ... Frame and rocking wall(FRW)structures have excellent resilient performance during earthquakes.However,the concrete at interfacial corners of rocking walls(RWs)is easily crushed due to local extreme compression during the rocking process.An innovative RW with a curved interface is proposed to prevent interfacial corners from producing local damage,enhancing its earthquake resilient performance(ERP).The precast wall panel with a curved interface is assembled into an integral self-centering hybrid rocking wall(SCRW)by two post-tensioned unbonded prestressed tendons.Moreover,two ordinary energy dissipation steel rebars and two shear reinforcements are arranged to increase the energy dissipation capacity and lateral resistance.Two SCRW specimens and one monolithic reinforced concrete(RC)shear wall(SW)were tested under pseudo-static loading to compare the ERPs of the proposed SCRW and the SW,focusing on studying the effect of the curved interface on the SCRW.The key resilient performance of rocking effects,failure modes,and hysteretic properties of the SCRW were explored.The results show that nonlinear deformations of the SCRW are concentrated along the interface between the SCRW and the foundation,avoiding damage within the SCRW.The restoring force provided by the prestressed tendons can effectively realize self-centering capacity with small residual deformation,and the resilient performance of the SCRW is better than that of monolithic SW.In addition,the curved interface of the SCRW makes the rocking center change and move inward,partially relieving the stress concentration and crush of concrete.The rocking range of the rocking center is about 41.4%of the width of the SCRW. 展开更多
关键词 self-centering hybrid rocking wall(SCRW) monolithic shear wall(SW) earthquake resilient performance(ERP) curved interface rocking center
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Progressive fragmentation of granular assemblies within rockslides: Insights from discrete-continuous numerical modeling
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作者 JIANG Hui ZHOU Yuande +2 位作者 WANG Jinting DU Xiuli HUANG Hailong 《Journal of Mountain Science》 SCIE CSCD 2024年第4期1174-1189,共16页
Rock fragmentation plays a critical role in rock avalanches,yet conventional approaches such as classical granular flow models or the bonded particle model have limitations in accurately characterizing the progressive... Rock fragmentation plays a critical role in rock avalanches,yet conventional approaches such as classical granular flow models or the bonded particle model have limitations in accurately characterizing the progressive disintegration and kinematics of multi-deformable rock blocks during rockslides.The present study proposes a discrete-continuous numerical model,based on a cohesive zone model,to explicitly incorporate the progressive fragmentation and intricate interparticle interactions inherent in rockslides.Breakable rock granular assemblies are released along an inclined plane and flow onto a horizontal plane.The numerical scenarios are established to incorporate variations in slope angle,initial height,friction coefficient,and particle number.The evolutions of fragmentation,kinematic,runout and depositional characteristics are quantitatively analyzed and compared with experimental and field data.A positive linear relationship between the equivalent friction coefficient and the apparent friction coefficient is identified.In general,the granular mass predominantly exhibits characteristics of a dense granular flow,with the Savage number exhibiting a decreasing trend as the volume of mass increases.The process of particle breakage gradually occurs in a bottom-up manner,leading to a significant increase in the angular velocities of the rock blocks with increasing depth.The simulation results reproduce the field observations of inverse grading and source stratigraphy preservation in the deposit.We propose a disintegration index that incorporates factors such as drop height,rock mass volume,and rock strength.Our findings demonstrate a consistent linear relationship between this index and the fragmentation degree in all tested scenarios. 展开更多
关键词 rock fragmentation rockSLIDE Numerical modelling Discrete-continuous modelling RUNOUT Cohesive zone model
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苦参碱调节RhoA-ROCK信号通路对冠心病模型大鼠Th17/Treg细胞平衡的影响 被引量:1
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作者 哈斯高娃 乌吉斯古楞 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第3期349-357,共9页
目的探讨苦参碱(Matrine)对冠心病(coronary heart disease,CHD)大鼠辅助T细胞17(helper T cell 17,Th17)/调节性T细胞(regulatory T cells,Treg)细胞平衡及Ras同源基因家族成员A(RhoA)-Rho相关的卷曲螺旋激酶(ROCK)信号通路的影响。方... 目的探讨苦参碱(Matrine)对冠心病(coronary heart disease,CHD)大鼠辅助T细胞17(helper T cell 17,Th17)/调节性T细胞(regulatory T cells,Treg)细胞平衡及Ras同源基因家族成员A(RhoA)-Rho相关的卷曲螺旋激酶(ROCK)信号通路的影响。方法建立冠心病模型,将实验大鼠分为对照组、模型组、苦参碱低剂量(50 mg·kg^(-1))组、苦参碱高剂量(200 mg·kg^(-1))组及苦参碱高剂量(200 mg·kg^(-1))+LPA组(10 mg·kg^(-1))。超声心动图进行大鼠心功能检测;酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法进行白细胞介素17(IL-17)、转化生长因子β(TGF-β)水平检测;流式细胞术检测Th17、Treg数量及Th17/Treg比值;免疫组化进行内皮型一氧化氮合酶(eNOS)、内皮素1(ET-1)蛋白表达水平检测;Masson染色进行大鼠心肌组织的病理形态变化观察;TTC染色检测各组大鼠心肌梗死情况;TUNEL染色进行心肌组织中细胞凋亡情况检测;试剂盒检测RhoA活性;Western Blot法进行半胱氨酸天冬氨酸蛋白酶3(Caspase-3)、B细胞淋巴瘤因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)、RhoA、ROCK1、ROCK2蛋白表达水平检测。结果与对照组比较,模型组心肌组织有大量蓝色胶原纤维沉积,左室舒张末期容积(left ventricular end-diastolic volume,LVEDV)、左室收缩末期容积(left ventricular end-systolic volume,LVESV)、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、TUNEL阳性率、Bax、Caspase-3、RhoA活性、RhoA、ROCK1、ROCK2表达水平明显升高,左室射血分数(left ventricular ejection fraction,LVEF)、左室缩短分数(left ventricular shortening fraction,LVFS)、TGF-β、Treg、eNOS、Bcl-2表达水平明显降低(P<0.05)。与模型组比较,Matrine-L组、苦参碱高剂量组心肌组织蓝色胶原纤维逐渐减少,LVEDV、LVESV、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、TUNEL阳性率、Bax、Caspase-3、RhoA活性、RhoA、ROCK1、ROCK2表达水平依次明显降低,LVEF、LVFS、TGF-β、Treg、eNOS、Bcl-2表达水平依次明显升高(P<0.05)。与苦参碱高剂量组比较,苦参碱高剂量+LPA组心肌组织蓝色胶原纤维增多,LVEDV、LVESV、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、TUNEL阳性率、Bax、Caspase-3、RhoA活性、RhoA、ROCK1、ROCK2表达水平明显升高,LVEF、LVFS、TGF-β、Treg、eNOS、Bcl-2表达水平明显降低(P<0.05)。结论苦参碱通过抑制RhoAROCK信号通路调节Th17/Treg细胞平衡,改善冠心病大鼠心肌损伤。 展开更多
关键词 苦参碱 冠心病 Ras同源基因家族成员A-Rho相关的卷曲螺旋激酶信号通路(RhoA-rock) 辅助T细胞17/调节性T细胞(Th17/Treg) 心肌损伤 大鼠
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Development and applications of the quasi‐dynamic triaxial apparatus for deep rocks
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作者 Jinzhi Luo Yanyan Cai +3 位作者 Jin Yu Jianzhi Zhang Yaoliang Zhu Yao Wei 《Deep Underground Science and Engineering》 2024年第1期70-90,共21页
The mechanical behaviors of deep rocks have always posed a challenge for the implementation and safe operation of major underground engineering projects.To this end,this study modified the existing mainstream rock mec... The mechanical behaviors of deep rocks have always posed a challenge for the implementation and safe operation of major underground engineering projects.To this end,this study modified the existing mainstream rock mechanics instruments equipped with a dynamic disturbance loading system and developed a second‐generation TFD‐2000/D triaxial instrument.The first‐generation device is equipped with an independent disturbance system and an advanced EDC‐580 all‐digital servo controller,which can apply disturbing load independently,implement the function of cyclic disturbance,and combine dynamic and static disturbances.The instrument was found to be reliable for use in analyzing the damage process of rocks in the disturbance test of marbles.The second‐generation instrument tackles three limitations of the first‐generation instrument:(i)it upgrades the strain measurement system and uses extensometers with linear variable differential transformers to accurately measure deformation;(ii)it uses the self‐balanced chamber to replace the Hoek–Franklin triaxial cell and auto‐balancing triaxial pressure chamber;and(iii)the loading rod is independently equipped with an EDC‐580 all‐digital servo controller,which measures precise loads.The experimental findings confirmed that the second‐generation instrument can be used for rock mechanics testing under cyclic disturbance loading,the disturbance–stress relaxation cycle,and the creep–fatigue cycle.In this sense,the second‐generation instrument can be a useful addition to deep rock mechanical instruments and provide a valuable reference. 展开更多
关键词 complex stress conditions deep rock disturbance loading modification of instrument rock mechanics
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