[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转...[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转录组测序,测序数据经短时间序列表达模式(STEM)趋势分析、功能富集分析和互作网络分析。[结果]40 kg vs 80 kg、80 kg vs 120 kg和40 kg vs 120 kg分别筛选到1517、486和1752个显著差异基因;差异基因STEM分析显示:模块4和1的基因先显著下调后基本不变,模块15、12和11的基因随体质量增加而显著上调,模块0的基因随体质量增加而显著下调;WNT10B、CPT1B和C5AR2位于模块4和1的基因网络核心,PLA2G7、WWTR1、SPP1、SERPINE1和PTPN11位于模块15、12和11的基因网络核心,ADIPOQ、CH25H和IL10位于模块0的基因网络核心;WNT10B和CPT1B等11个基因的qPCR验证结果与转录组测序结果一致。[结论]WNT10B和PTPN11等11个核心基因以协作方式参与大型迪庆藏猪腹膜脂肪代谢调控,结果可为迪庆藏猪的遗传改良提供参考。展开更多
To assess the genetic diversity between randomly and selectively bred populations,we sequenced 438 bp of the mitochondrial DNA control region from 102 pigs.These samples represented four native pig breeds,one nucleus ...To assess the genetic diversity between randomly and selectively bred populations,we sequenced 438 bp of the mitochondrial DNA control region from 102 pigs.These samples represented four native pig breeds,one nucleus and one conservation herd from Yunnan,China.Twenty haplotypes with sixteen polymorphic sites were identified.The number of haplotypes in the nucleus herd of Saba pig and the conservation herd of Banna miniature pig were restricted to three and one,respectively,while the randomly bred pig populations exhibited over six haplotypes.Notably,haplotype diversity in randomly bred populations was significantly greater than the selectively bred populations（h=0.732 vs.0.425 and 0,exact test,P≤0.0036）.These findings demonstrate that selective breeding generated low genetic diversity compared to randomly bred pig breeds.A timely intervention and well programmed breeding approach would stop further genetic diversity reduction in the nucleus and conservation herds of native pig breeds.Otherwise,selective breeding would dramatically reduce genetic diversity in only several years,indicating that sharp contradictions exist between breeding,conservation and genetic diversity.Genetic relationships are discussed based on net genetic distances among pig populations.展开更多
文摘[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转录组测序,测序数据经短时间序列表达模式(STEM)趋势分析、功能富集分析和互作网络分析。[结果]40 kg vs 80 kg、80 kg vs 120 kg和40 kg vs 120 kg分别筛选到1517、486和1752个显著差异基因;差异基因STEM分析显示:模块4和1的基因先显著下调后基本不变,模块15、12和11的基因随体质量增加而显著上调,模块0的基因随体质量增加而显著下调;WNT10B、CPT1B和C5AR2位于模块4和1的基因网络核心,PLA2G7、WWTR1、SPP1、SERPINE1和PTPN11位于模块15、12和11的基因网络核心,ADIPOQ、CH25H和IL10位于模块0的基因网络核心;WNT10B和CPT1B等11个基因的qPCR验证结果与转录组测序结果一致。[结论]WNT10B和PTPN11等11个核心基因以协作方式参与大型迪庆藏猪腹膜脂肪代谢调控,结果可为迪庆藏猪的遗传改良提供参考。
基金supported by research grants of the National Basic Research Program of China (2007CB815700 2006CB102100)Bureau of Science and Technology of Yunnan Province, and Natural Science Foundation of China (30621092)
文摘To assess the genetic diversity between randomly and selectively bred populations,we sequenced 438 bp of the mitochondrial DNA control region from 102 pigs.These samples represented four native pig breeds,one nucleus and one conservation herd from Yunnan,China.Twenty haplotypes with sixteen polymorphic sites were identified.The number of haplotypes in the nucleus herd of Saba pig and the conservation herd of Banna miniature pig were restricted to three and one,respectively,while the randomly bred pig populations exhibited over six haplotypes.Notably,haplotype diversity in randomly bred populations was significantly greater than the selectively bred populations（h=0.732 vs.0.425 and 0,exact test,P≤0.0036）.These findings demonstrate that selective breeding generated low genetic diversity compared to randomly bred pig breeds.A timely intervention and well programmed breeding approach would stop further genetic diversity reduction in the nucleus and conservation herds of native pig breeds.Otherwise,selective breeding would dramatically reduce genetic diversity in only several years,indicating that sharp contradictions exist between breeding,conservation and genetic diversity.Genetic relationships are discussed based on net genetic distances among pig populations.
