The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum(LCDV-rc) was amplified and analysed.The 457bp DNA core fragment was amplified with the degenerate primers designed according to...The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum(LCDV-rc) was amplified and analysed.The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses,then the flaking sequences adjacent to the core region were amplified by inverse PCR,and the complete sequence was obtained by combining all of them.The open reading frame of the gene is 1380bp in length,encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87.Constructing the phylogenetic tree for comparing the MCP amino acid of iridoviruses,the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch.Accordingly LCDV-rc is identified as Lymphocystivirus.展开更多
[目的]分析了解乌鳢结节病的病原。[方法]以患病乌鳢为研究材料,通过组织病理学观察和人工感染试验,确定乌鳢结节病的病原性质;通过基本生理生化特性、16 S rRNA基因部分序列和药物敏感性的测定,对乌鳢结节病的病原进行鉴定。[结果]组...[目的]分析了解乌鳢结节病的病原。[方法]以患病乌鳢为研究材料,通过组织病理学观察和人工感染试验,确定乌鳢结节病的病原性质;通过基本生理生化特性、16 S rRNA基因部分序列和药物敏感性的测定,对乌鳢结节病的病原进行鉴定。[结果]组织病理学观察显示典型结节的中央为干酪样坏死,内含坏死的组织细胞和白细胞,其间有大量呈长杆状或分枝状菌丝;从病鱼的肾脏、肝脏等多个组织及腹水中均分离培养出细菌,用分离菌株作回归感染,证实分离菌株为乌鳢结节病的病原菌;通过对病原菌的基本生理生化特性测定和16 S rRNA基因部分序列分析,初步确定乌鳢结节病的病原为诺卡氏菌属的鰤诺卡氏菌(Nocardia seriolae)。[结论]该研究为乌鳢结节病的进一步研究奠定了基础。展开更多
文摘The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum(LCDV-rc) was amplified and analysed.The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses,then the flaking sequences adjacent to the core region were amplified by inverse PCR,and the complete sequence was obtained by combining all of them.The open reading frame of the gene is 1380bp in length,encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87.Constructing the phylogenetic tree for comparing the MCP amino acid of iridoviruses,the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch.Accordingly LCDV-rc is identified as Lymphocystivirus.
文摘[目的]分析了解乌鳢结节病的病原。[方法]以患病乌鳢为研究材料,通过组织病理学观察和人工感染试验,确定乌鳢结节病的病原性质;通过基本生理生化特性、16 S rRNA基因部分序列和药物敏感性的测定,对乌鳢结节病的病原进行鉴定。[结果]组织病理学观察显示典型结节的中央为干酪样坏死,内含坏死的组织细胞和白细胞,其间有大量呈长杆状或分枝状菌丝;从病鱼的肾脏、肝脏等多个组织及腹水中均分离培养出细菌,用分离菌株作回归感染,证实分离菌株为乌鳢结节病的病原菌;通过对病原菌的基本生理生化特性测定和16 S rRNA基因部分序列分析,初步确定乌鳢结节病的病原为诺卡氏菌属的鰤诺卡氏菌(Nocardia seriolae)。[结论]该研究为乌鳢结节病的进一步研究奠定了基础。
