目的:考察骨钙素(BGP)Hind III基因多态性是否与中国绝经前妇女空腹血糖(FPG)变异间存在关联;检测BGP基因型和体重指数(BMI)间的关联是否可以部分地解释BGP基因型与FPG间的关联。方法:研究对象是328名中国绝经前妇女,年龄21岁以上[(33.2...目的:考察骨钙素(BGP)Hind III基因多态性是否与中国绝经前妇女空腹血糖(FPG)变异间存在关联;检测BGP基因型和体重指数(BMI)间的关联是否可以部分地解释BGP基因型与FPG间的关联。方法:研究对象是328名中国绝经前妇女,年龄21岁以上[(33.2±5.9)岁],FPG值平均为4.92 mmol/L(标准差,0.81),采用聚合酶链反应(PCR)-限制性片段长度多态(RFLP)法对所有研究对象的BGP Hind III位点进行基因分型。结果:BGP Hind III多态性与校正年龄的FPG以及未用年龄校正的FPG间均存在显著的关联(P值分别为0.028和0.041),且BGP基因可以解释3.32%的FPG变异。但BGP Hind III多态性与年龄和BMI校正的FPG间无显著关联(P=0.517)。此外,BGP Hind III多态性与未校正的BMI(P=0.002)或年龄校正的BMI(P=0.003)都有显著的关联,且BMI与未校正的FPG也存在显著相关(r=0.424,P<0.01),BMI可以解释20.15%的FPG变异。结论:BMI可影响BGP Hind III多态性与中国绝经前妇女FPG间的关联。该研究结果提示协变量如BMI在FPG的遗传关联研究中的重要性。展开更多
Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and eluci...Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and elucidated, except for inflammatory reaction. Here we analyzed the proteomics of kidney of rats after adenine overloading using LS-MS/MS assay, and observed the role of anemoside B4(B4). The results showed that adenine could down-regulate 285 proteins and up-regulate 164 proteins in rat kidney tissue compared with the normal group. Down-regulated proteins mainly affected related pathways, such as energy metabolism, while up-regulated proteins affected inflammatory response pathways and metabolic pathways. B4 could significantly reverse the down-regulation of about 40 proteins, which were involved in mitochondria, redox processes, extracellular exosomes, acetylation and other signaling pathways. Simultaneously, B4 could inhibit the up-regulation of five proteins caused by adenine, which were involved in cell cycle, oocyte meiosis, PI3 K-Akt and other signaling pathways. Further experimental results of mRNA expression using real-time PCR assay supported the proteomic analysis. Therefore, we proposed that the damage of rat kidney caused by adenine was more complicated, not only with an inflammatory reaction, but also with extensive effects to various metabolic processes in the body. This work provided a valuable clue for comprehensive understanding of adenine-induced renal damage.展开更多
文摘目的:考察骨钙素(BGP)Hind III基因多态性是否与中国绝经前妇女空腹血糖(FPG)变异间存在关联;检测BGP基因型和体重指数(BMI)间的关联是否可以部分地解释BGP基因型与FPG间的关联。方法:研究对象是328名中国绝经前妇女,年龄21岁以上[(33.2±5.9)岁],FPG值平均为4.92 mmol/L(标准差,0.81),采用聚合酶链反应(PCR)-限制性片段长度多态(RFLP)法对所有研究对象的BGP Hind III位点进行基因分型。结果:BGP Hind III多态性与校正年龄的FPG以及未用年龄校正的FPG间均存在显著的关联(P值分别为0.028和0.041),且BGP基因可以解释3.32%的FPG变异。但BGP Hind III多态性与年龄和BMI校正的FPG间无显著关联(P=0.517)。此外,BGP Hind III多态性与未校正的BMI(P=0.002)或年龄校正的BMI(P=0.003)都有显著的关联,且BMI与未校正的FPG也存在显著相关(r=0.424,P<0.01),BMI可以解释20.15%的FPG变异。结论:BMI可影响BGP Hind III多态性与中国绝经前妇女FPG间的关联。该研究结果提示协变量如BMI在FPG的遗传关联研究中的重要性。
基金National Innovative Drugs 13th Five-Year Major Special Project of China(Grant No.2018ZX09301030-002)
文摘Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and elucidated, except for inflammatory reaction. Here we analyzed the proteomics of kidney of rats after adenine overloading using LS-MS/MS assay, and observed the role of anemoside B4(B4). The results showed that adenine could down-regulate 285 proteins and up-regulate 164 proteins in rat kidney tissue compared with the normal group. Down-regulated proteins mainly affected related pathways, such as energy metabolism, while up-regulated proteins affected inflammatory response pathways and metabolic pathways. B4 could significantly reverse the down-regulation of about 40 proteins, which were involved in mitochondria, redox processes, extracellular exosomes, acetylation and other signaling pathways. Simultaneously, B4 could inhibit the up-regulation of five proteins caused by adenine, which were involved in cell cycle, oocyte meiosis, PI3 K-Akt and other signaling pathways. Further experimental results of mRNA expression using real-time PCR assay supported the proteomic analysis. Therefore, we proposed that the damage of rat kidney caused by adenine was more complicated, not only with an inflammatory reaction, but also with extensive effects to various metabolic processes in the body. This work provided a valuable clue for comprehensive understanding of adenine-induced renal damage.
