目的研究口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)在人结肠癌组织中的表达变化并探讨其对结肠癌细胞增殖能力、细胞周期及早期凋亡的影响。方法收集人结肠癌及癌旁组织标本,分别以qRT-PCR及Western blot检测ORA...目的研究口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)在人结肠癌组织中的表达变化并探讨其对结肠癌细胞增殖能力、细胞周期及早期凋亡的影响。方法收集人结肠癌及癌旁组织标本,分别以qRT-PCR及Western blot检测ORAOV1在mRNA及蛋白水平的表达变化情况;采用RNAi技术获得ORAOV1低表达的Lo Vo及SW480细胞株,并使用Western blot对干扰结果进行鉴定;采用CCK-8检测干扰ORAOV1表达后两种结肠癌细胞增殖能力的变化;并采用流式细胞术检测干扰ORAOV1表达对结肠癌细胞周期及早期凋亡的影响。结果 qRT-PCR及Western blot结果分别显示结肠癌组织中的ORAOV1 mRNA及蛋白水平高于癌旁组织(P<0.05);Western blot检测证实Lo Vo及SW480细胞中ORAOV1干扰成功;在这两种结肠癌细胞株中,CCK-8检测结果显示,干扰ORAOV1可抑制细胞增殖能力;流式细胞术结果显示,干扰ORAOV1可使处于S期比例增加(P<0.05)、早期凋亡比例增加(P<0.05)。结论结肠癌组织中的ORAOV1表达增加,干扰ORAOV1可降低结肠癌细胞的增殖能力,可能与S期阻滞及细胞早期凋亡增加有关。展开更多
磷肥利用率低,烟地土壤长期施用化肥导致磷肥效益低下和土壤肥力退化。为了合理施肥、提升肥效,改善土壤肥力和烟草品质,进行了肽肥供氮下,减施磷肥的土壤盆栽试验。以云烟87和多年种烟土壤为材料,在与烟草专用肥等氮的肽肥用量下,设置...磷肥利用率低,烟地土壤长期施用化肥导致磷肥效益低下和土壤肥力退化。为了合理施肥、提升肥效,改善土壤肥力和烟草品质,进行了肽肥供氮下,减施磷肥的土壤盆栽试验。以云烟87和多年种烟土壤为材料,在与烟草专用肥等氮的肽肥用量下,设置了6种施磷比列,研究减施磷肥对烟草生长的影响。结果表明,肽肥控氮下,适当减施磷肥可增加云烟87株高、最大叶长和叶宽、叶片数、伸根期和旺长期的叶绿素水平,并能增加烟叶产量。肽肥控氮减施磷肥对株高的增加率在47%~91%之间,株高、伸根期叶绿素水平、烟叶产量均与施磷比例呈极显著的二次函数相关关系,而旺长期烟叶叶绿素水平与施磷比例呈极显著的线性负相关。所有处理对云烟87成熟期叶绿素水平无显著影响,说明肽肥控氮减施磷肥不会延迟烟叶的成熟。总之,肽肥因其供氮平稳,能改善土壤环境,提升土壤磷有效性而使减施磷肥能促进烟草生长,综合所测生长指标,以施用对照磷的12%和16%对烟草生长的促进效果最好。The low utilization rate of phosphorus fertilizer and long-term application of chemical fertilizers in tobacco fields leads to low phosphorus fertilizer efficiency and soil fertility degradation. In order to apply fertilizer reasonably, improve fertilizer efficiency, soil fertility and tobacco quality, a soil pot experiment was conducted with supplying nitrogen by peptide fertilizer and reduced phosphorus fertilizer application. Using Yunyan 87 and long term tobacco planting soil as materials, 6 phosphorus application ratio was set up under the same nitrogen of peptide fertilizer as tobacco specific fertilizer, to study the effect of reducing phosphorus fertilizer application on tobacco growth. The results showed that under nitrogen controlled by peptide fertilizer, appropriately decrement of phosphorus fertilizer can increase the height, maximum leaf length and width, the number of leaves, leaf chlorophyll levels of Yunyan 87 at root extension and vigorous growing period, and the tobacco yield. The increase rate of plant height by reducing the application of phosphorus fertilizer through nitrogen control with peptide fertilizer ranges from 47% to 91%. There is a highly significant quadratic correlation between plant height, chlorophyll level during root extension period, and tobacco yield with phosphorus application ratio. However, there is a highly significant linear negative correlation between chlorophyll level and phosphorus application ratio in vigorous growth period of tobacco. All treatments had no significant effect on the chlorophyll level during the mature stage of Yunyan 87, indicating that nitrogen controlled by peptide fertilizer and reduced phosphorus fertilizer did not delay the maturity of tobacco leaves. In summary, peptide fertilizer, due to its stable nitrogen supply, improving soil environment and enhancing soil phosphorus availability, reducing the application of phosphorus fertilizer can promote tobacco growth. Based on the measured growth indicators, applying 12% and 16% of control phosphorus has the best promoting effect on tobacco growth.展开更多
文摘磷肥利用率低,烟地土壤长期施用化肥导致磷肥效益低下和土壤肥力退化。为了合理施肥、提升肥效,改善土壤肥力和烟草品质,进行了肽肥供氮下,减施磷肥的土壤盆栽试验。以云烟87和多年种烟土壤为材料,在与烟草专用肥等氮的肽肥用量下,设置了6种施磷比列,研究减施磷肥对烟草生长的影响。结果表明,肽肥控氮下,适当减施磷肥可增加云烟87株高、最大叶长和叶宽、叶片数、伸根期和旺长期的叶绿素水平,并能增加烟叶产量。肽肥控氮减施磷肥对株高的增加率在47%~91%之间,株高、伸根期叶绿素水平、烟叶产量均与施磷比例呈极显著的二次函数相关关系,而旺长期烟叶叶绿素水平与施磷比例呈极显著的线性负相关。所有处理对云烟87成熟期叶绿素水平无显著影响,说明肽肥控氮减施磷肥不会延迟烟叶的成熟。总之,肽肥因其供氮平稳,能改善土壤环境,提升土壤磷有效性而使减施磷肥能促进烟草生长,综合所测生长指标,以施用对照磷的12%和16%对烟草生长的促进效果最好。The low utilization rate of phosphorus fertilizer and long-term application of chemical fertilizers in tobacco fields leads to low phosphorus fertilizer efficiency and soil fertility degradation. In order to apply fertilizer reasonably, improve fertilizer efficiency, soil fertility and tobacco quality, a soil pot experiment was conducted with supplying nitrogen by peptide fertilizer and reduced phosphorus fertilizer application. Using Yunyan 87 and long term tobacco planting soil as materials, 6 phosphorus application ratio was set up under the same nitrogen of peptide fertilizer as tobacco specific fertilizer, to study the effect of reducing phosphorus fertilizer application on tobacco growth. The results showed that under nitrogen controlled by peptide fertilizer, appropriately decrement of phosphorus fertilizer can increase the height, maximum leaf length and width, the number of leaves, leaf chlorophyll levels of Yunyan 87 at root extension and vigorous growing period, and the tobacco yield. The increase rate of plant height by reducing the application of phosphorus fertilizer through nitrogen control with peptide fertilizer ranges from 47% to 91%. There is a highly significant quadratic correlation between plant height, chlorophyll level during root extension period, and tobacco yield with phosphorus application ratio. However, there is a highly significant linear negative correlation between chlorophyll level and phosphorus application ratio in vigorous growth period of tobacco. All treatments had no significant effect on the chlorophyll level during the mature stage of Yunyan 87, indicating that nitrogen controlled by peptide fertilizer and reduced phosphorus fertilizer did not delay the maturity of tobacco leaves. In summary, peptide fertilizer, due to its stable nitrogen supply, improving soil environment and enhancing soil phosphorus availability, reducing the application of phosphorus fertilizer can promote tobacco growth. Based on the measured growth indicators, applying 12% and 16% of control phosphorus has the best promoting effect on tobacco growth.
