近年来,我国信鸽和肉鸽养殖业发展迅猛,已经成为我国继鸡、鸭、鹅之后的第四大家禽产业。鸽腺病毒(pigeon adenovirus,PiAdV)是鸽群中常见的一种病原,国际病毒分类委员会(International Committee on Taxonomy of Viruses,ICTV)将鸽腺...近年来,我国信鸽和肉鸽养殖业发展迅猛,已经成为我国继鸡、鸭、鹅之后的第四大家禽产业。鸽腺病毒(pigeon adenovirus,PiAdV)是鸽群中常见的一种病原,国际病毒分类委员会(International Committee on Taxonomy of Viruses,ICTV)将鸽腺病毒分为PiAdV-A和PiAdV-B。2022年1月起,我国新疆、河北、北京等地区的信鸽公棚以及肉鸽养殖场暴发了一种以鸽肝组织弥漫性坏死和突然死亡为特征的新型鸽腺病毒病。该病在肉鸽场传播缓慢,持续时间长,发病种鸽消瘦、突然死亡,病程长达3个月,累计死亡率15%,给养鸽业造成了严重的经济损失。在信鸽公棚,主要是5-6月龄信鸽发病,病程较短,表现为采食量下降,死亡率10%-13%,严重影响信鸽的比赛成绩。经病理剖检、组织病理学、电镜观察和病原核酸检测等方法综合诊断为鸽腺病毒A型感染。展开更多
用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,1...用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,10号白油为佐剂,制成ND-IB-EDS76-AE四联灭活疫苗。对四联苗各项技术指标进行了测定,证明本疫苗安全、无任何副作用;免疫10-14 d后产生免疫力;ND部分效检,攻毒100％保护,每羽份含ND效价达50-123 PD50;IB部分效检,免疫21-28 d后攻毒保护率达80％-100％,IB HI效价≥1:64;EDS76效检,免疫后21 d HI效价≥1:128;AE效检,保护率达80％-100％。展开更多
[Objective] The aim of this study was to realize efficient expression of the porcine reproductive and respiratory syndrome virus （PRRSV） ORF7 gene in genetic engineering bacteria and analYze the immunological activi...[Objective] The aim of this study was to realize efficient expression of the porcine reproductive and respiratory syndrome virus （PRRSV） ORF7 gene in genetic engineering bacteria and analYze the immunological activity of the recombinant protein after purification. [ Method] The constructed recombinant expression vector pET-ORF7 was transformed into Escherichia co1BL21 （DE3） and induced by IPTG under the optimal condition. After analysis of SDS-PAGE and Western Blot, the expression products were purified by Ni-NTA His · Bind Resin chrom- atographic column under denaturing condition and renatured by gradient dialysis. Subsequently, the immunological activity of the renatured recombinant protein was detected by Westem Blot and indirect ELISA. [ Result] The recombinant plasmid pET-ORF7 expressed in E. coli successfully, and the fusion protein was in the form of inclusion body. By SDS-PAGE detection, the molecular weight of the expression protein was approximate 33 kD, according with the expectation. Analysis by Bandscan software showed that the expressed fusion protein was about 50% of total bacterial protein of BL21 （DE3）. Wastem Blot and indirect ELISA detection showed that the renatured protein could react with PRRSV positive serum specifically, indicating its good immunological activity. [ Conclusion] This study lays a foundation for the preparation of PRRSV monoclonal antibody and diagnostic kit.展开更多
[ Objective] The aim of this study was to provide a theoretical basis for the prevention and treatment of highly pathogenic porcine reproductive and respiratory syndrome （HP-PRRS）. [Method] Antigen location and hist...[ Objective] The aim of this study was to provide a theoretical basis for the prevention and treatment of highly pathogenic porcine reproductive and respiratory syndrome （HP-PRRS）. [Method] Antigen location and histopathological observation in natural cases infected by highly pathogenic porcine reproductive and respiratory syndrome virus （HP-PRRSV） were analyzed by immunohistochemistry and H. E. staining. [Result] The virus antigen mainly existed in epithelial calls, and also a few in mecrophages, lymphocytes and brain nerve cells. [ Conclusion] The cell and tissue tropism of HP-PRRSV strain in natural cases is different from that of previous strains.展开更多
文摘近年来,我国信鸽和肉鸽养殖业发展迅猛,已经成为我国继鸡、鸭、鹅之后的第四大家禽产业。鸽腺病毒(pigeon adenovirus,PiAdV)是鸽群中常见的一种病原,国际病毒分类委员会(International Committee on Taxonomy of Viruses,ICTV)将鸽腺病毒分为PiAdV-A和PiAdV-B。2022年1月起,我国新疆、河北、北京等地区的信鸽公棚以及肉鸽养殖场暴发了一种以鸽肝组织弥漫性坏死和突然死亡为特征的新型鸽腺病毒病。该病在肉鸽场传播缓慢,持续时间长,发病种鸽消瘦、突然死亡,病程长达3个月,累计死亡率15%,给养鸽业造成了严重的经济损失。在信鸽公棚,主要是5-6月龄信鸽发病,病程较短,表现为采食量下降,死亡率10%-13%,严重影响信鸽的比赛成绩。经病理剖检、组织病理学、电镜观察和病原核酸检测等方法综合诊断为鸽腺病毒A型感染。
文摘用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,10号白油为佐剂,制成ND-IB-EDS76-AE四联灭活疫苗。对四联苗各项技术指标进行了测定,证明本疫苗安全、无任何副作用;免疫10-14 d后产生免疫力;ND部分效检,攻毒100％保护,每羽份含ND效价达50-123 PD50;IB部分效检,免疫21-28 d后攻毒保护率达80％-100％,IB HI效价≥1:64;EDS76效检,免疫后21 d HI效价≥1:128;AE效检,保护率达80％-100％。
文摘[Objective] The aim of this study was to realize efficient expression of the porcine reproductive and respiratory syndrome virus （PRRSV） ORF7 gene in genetic engineering bacteria and analYze the immunological activity of the recombinant protein after purification. [ Method] The constructed recombinant expression vector pET-ORF7 was transformed into Escherichia co1BL21 （DE3） and induced by IPTG under the optimal condition. After analysis of SDS-PAGE and Western Blot, the expression products were purified by Ni-NTA His · Bind Resin chrom- atographic column under denaturing condition and renatured by gradient dialysis. Subsequently, the immunological activity of the renatured recombinant protein was detected by Westem Blot and indirect ELISA. [ Result] The recombinant plasmid pET-ORF7 expressed in E. coli successfully, and the fusion protein was in the form of inclusion body. By SDS-PAGE detection, the molecular weight of the expression protein was approximate 33 kD, according with the expectation. Analysis by Bandscan software showed that the expressed fusion protein was about 50% of total bacterial protein of BL21 （DE3）. Wastem Blot and indirect ELISA detection showed that the renatured protein could react with PRRSV positive serum specifically, indicating its good immunological activity. [ Conclusion] This study lays a foundation for the preparation of PRRSV monoclonal antibody and diagnostic kit.
文摘[ Objective] The aim of this study was to provide a theoretical basis for the prevention and treatment of highly pathogenic porcine reproductive and respiratory syndrome （HP-PRRS）. [Method] Antigen location and histopathological observation in natural cases infected by highly pathogenic porcine reproductive and respiratory syndrome virus （HP-PRRSV） were analyzed by immunohistochemistry and H. E. staining. [Result] The virus antigen mainly existed in epithelial calls, and also a few in mecrophages, lymphocytes and brain nerve cells. [ Conclusion] The cell and tissue tropism of HP-PRRSV strain in natural cases is different from that of previous strains.
