Optimization of Callus Induction Medium for Schisandga chinensis Baill via Uniform Design

[Objective]This study was to optimize callus induction medium for Schisandga chinensis Baill.[Method]Using callus induction rate as an indicator,uniform design was employed to optimize hormone combination at poly-factors and poly-levels for callus induction from Schisandga chinensis Baill.[Results]Optimal hormone combinations depended on different explants：optimum medium for both tender leaf and petiole was MS ＋3 mg/L 6-BA,for stem segment was MS ＋3 mg/L 6-BA ＋0.6 mg/L NAA.[Conclusion]Uniform design is a time-saving and convenient method for the optimum medium for callus and yields a higher callus induction rate.

筛选北五味子愈伤组织诱导中不同因素的最佳条件

为筛选北五味子组织培养中愈伤组织诱导的最佳条件,以叶片、叶柄、茎为外植体,探讨不同因素中不同条件对北五味子组织培养中愈伤组织诱导的影响.结果表明:0.1%HgCl2适合作为北五味子的消毒剂,其中叶片、叶柄和茎的最佳消毒时间为6min、6min和10min.叶片、叶柄、茎3个外植体的最适基本培养基分别为MS、B5和N6;最佳外植体为茎段.