液泡分选相关蛋白28(Vacuolar Protein Sorting-associated Protein 28,VPS28)是内吞体分选转运复合体I(Endosomal Sorting Complex Required for Transport I,ESCRTI)的组成亚基之一,通过控制细胞内多泡体(MVBs)的形成来参与长链脂肪...液泡分选相关蛋白28(Vacuolar Protein Sorting-associated Protein 28,VPS28)是内吞体分选转运复合体I(Endosomal Sorting Complex Required for Transport I,ESCRTI)的组成亚基之一,通过控制细胞内多泡体(MVBs)的形成来参与长链脂肪酸、甘油三酯和分泌蛋白的转运。为探究VPS28基因在色瓦绵羊育种繁殖中的作用,本实验克隆了色瓦绵羊VPS28基因并进行生物信息学分析,测定该基因在乳腺、肌肉、心脏、小肠等10个组织中的表达。结果显示:VPS28基因编码区长度为666 bp;VPS28蛋白序列全长221个氨基酸,相对分子质量为25484.25;等电点为5.37,是细胞内一种酸性亲水性蛋白,不具跨膜结构。VPS28基因在色瓦绵羊乳腺、心脏、小肠等10个组织中都有表达,其中在乳腺和小肠中的表达量显著高于肌肉、心脏等其他组织(P<0.0001),在肌肉组织中的表达量最低。研究结果表明:VPS28基因在乳腺组织中高表达,可能与色瓦绵羊产奶性状相关。这一研究结果为VPS28基因在色瓦绵羊产奶性状的功能研究提供了参考依据。展开更多
[目的]肉羊的宰前活重和屠宰性状直接影响其经济价值,本研究分析多羔绵羊新品种群的屠宰性状及其与宰前活重的关系,对新品种选育和生产推广具有参考作用。[方法]测定6、12月龄(各月龄20只羊,公母各半)多羔绵羊的宰前活重(live body weig...[目的]肉羊的宰前活重和屠宰性状直接影响其经济价值,本研究分析多羔绵羊新品种群的屠宰性状及其与宰前活重的关系,对新品种选育和生产推广具有参考作用。[方法]测定6、12月龄(各月龄20只羊,公母各半)多羔绵羊的宰前活重(live body weight,LBW)、胴体重(carcass weight,CW)、净肉重(meat weight,MW)和眼肌面积(eye muscle area,EMA)。对3项屠宰性状与宰前活重进行线性回归分析,分别建立活重与3项屠宰性状间的回归关系。[结果]公母羊屠宰率均在50%以上,除屠宰率外,多羔绵羊12月龄的屠宰性状均高于6月龄。6月龄多羔绵羊宰前活重和屠宰率相比国内其他优良绵羊品种均属较高水平。在各月龄间,活重与胴体重、净肉重和眼肌面积间存在极显著的回归关系。[结论]多羔绵羊生长发育快、产肉性能高,可用宰前活重估算各月龄的相关屠宰性状值。展开更多
Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty...Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty goats was given subcutaneous injections of the purified inhibin 2 mg at neck.The goats were given boosterinjections of the inhibin 1 mg that was mixed with ureasodiumacetate and Freund incomplete adjuvant after 30 and 50 days.Five goats in control group were given admixture of urea and sodium acetate and adjuvant at the same time.The goats were injected with prostaglandin F2a 005 mg at 21 d and 31 d after the last immunization.The blood samples were collected at 30 d,51 d and 60 d after the first immunization and before immunization.The plasma FSH concentrates was assayed with RIA and the inhibin antibody titer was assayed with agar plate diffusion method.The plasma antibody titer was 1∶16256 at 60 d after first immunization.The plasma FSH concentrates of the immunized goats was higher than those of the control group(202 vs.12 mIU/ml,PZ<005).The number of follicle and the number of corpora lutea of the immunized group were as twice as those of the control(1931 vs.1223 and 225 vs.110,respectively).It indicated that inhibin purified from swine ovarian fluid could induce goat produce high ovulation rate and it can improve reproductive traits of goat.展开更多
文摘液泡分选相关蛋白28(Vacuolar Protein Sorting-associated Protein 28,VPS28)是内吞体分选转运复合体I(Endosomal Sorting Complex Required for Transport I,ESCRTI)的组成亚基之一,通过控制细胞内多泡体(MVBs)的形成来参与长链脂肪酸、甘油三酯和分泌蛋白的转运。为探究VPS28基因在色瓦绵羊育种繁殖中的作用,本实验克隆了色瓦绵羊VPS28基因并进行生物信息学分析,测定该基因在乳腺、肌肉、心脏、小肠等10个组织中的表达。结果显示:VPS28基因编码区长度为666 bp;VPS28蛋白序列全长221个氨基酸,相对分子质量为25484.25;等电点为5.37,是细胞内一种酸性亲水性蛋白,不具跨膜结构。VPS28基因在色瓦绵羊乳腺、心脏、小肠等10个组织中都有表达,其中在乳腺和小肠中的表达量显著高于肌肉、心脏等其他组织(P<0.0001),在肌肉组织中的表达量最低。研究结果表明:VPS28基因在乳腺组织中高表达,可能与色瓦绵羊产奶性状相关。这一研究结果为VPS28基因在色瓦绵羊产奶性状的功能研究提供了参考依据。
文摘Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty goats was given subcutaneous injections of the purified inhibin 2 mg at neck.The goats were given boosterinjections of the inhibin 1 mg that was mixed with ureasodiumacetate and Freund incomplete adjuvant after 30 and 50 days.Five goats in control group were given admixture of urea and sodium acetate and adjuvant at the same time.The goats were injected with prostaglandin F2a 005 mg at 21 d and 31 d after the last immunization.The blood samples were collected at 30 d,51 d and 60 d after the first immunization and before immunization.The plasma FSH concentrates was assayed with RIA and the inhibin antibody titer was assayed with agar plate diffusion method.The plasma antibody titer was 1∶16256 at 60 d after first immunization.The plasma FSH concentrates of the immunized goats was higher than those of the control group(202 vs.12 mIU/ml,PZ<005).The number of follicle and the number of corpora lutea of the immunized group were as twice as those of the control(1931 vs.1223 and 225 vs.110,respectively).It indicated that inhibin purified from swine ovarian fluid could induce goat produce high ovulation rate and it can improve reproductive traits of goat.
