本实验选用耐性菜豆85CT-49762为实验材料,运用膜 Mg^(2+)-ATP 酶电镜细胞化学方法,研究了细胞热锻炼前后膜系统热损伤的特点。结果表明:①细胞质的热凝固可能是膜热破坏的原因之一;②液泡膜比质膜对热更加敏感;③细胞膜系统上 ATP 酶...本实验选用耐性菜豆85CT-49762为实验材料,运用膜 Mg^(2+)-ATP 酶电镜细胞化学方法,研究了细胞热锻炼前后膜系统热损伤的特点。结果表明:①细胞质的热凝固可能是膜热破坏的原因之一;②液泡膜比质膜对热更加敏感;③细胞膜系统上 ATP 酶失活不是导致膜热破损的主要原因。展开更多
A cDNA Library was constructed with the heat shocked tomato ( Lycopersicon esculentum Mill.) flowers and then was screened with the probes of mitochondrial and endoplasmic reticulum conservative regions that were clon...A cDNA Library was constructed with the heat shocked tomato ( Lycopersicon esculentum Mill.) flowers and then was screened with the probes of mitochondrial and endoplasmic reticulum conservative regions that were cloned by using RT-PCR. The complete cDNAs of mitochondrial and endoplasmic reticulum small heat shock protein ( shsp) were selected out from the cDNA library. Furthermore, the temperature responses of these shsp genes were determined. Northern hybridization showed that the heat response temperatures of both genes in tomato flower were lower than that in leaf and that mitochondria shsp in leaf was cold-inducible. In this paper, the molecular features of the cloned genes, the causes of the uncommon heat response temperatures of sHSP in newer and the cold inducible character of mitochondria shsp gene in leaf were discussed.展开更多
Inspite of the large amount of low molecular weight heat shock protein (LMW HSP) present in plant, its function has still not been clearly known. Understanding the distribution and movement of LMW HSP in cells could p...Inspite of the large amount of low molecular weight heat shock protein (LMW HSP) present in plant, its function has still not been clearly known. Understanding the distribution and movement of LMW HSP in cells could provide useful information about its biological functions. A 14 kD HSP was purified from the microsome isolated from the bean of a highly thermotolerant plant Phaseolus vulgaris. Antiserum against this protein was preparaed. The localization of the protein in the cell was analysed by means of electromicroscopic immunogold labelling method. The images of electromicrograph showed that 14 kD HSP mainly existed in both cytoplasm and endoplasmic reticulum and that no labelled gold was found in tonoplasma, mitochondria or cell wall.展开更多
文摘A cDNA Library was constructed with the heat shocked tomato ( Lycopersicon esculentum Mill.) flowers and then was screened with the probes of mitochondrial and endoplasmic reticulum conservative regions that were cloned by using RT-PCR. The complete cDNAs of mitochondrial and endoplasmic reticulum small heat shock protein ( shsp) were selected out from the cDNA library. Furthermore, the temperature responses of these shsp genes were determined. Northern hybridization showed that the heat response temperatures of both genes in tomato flower were lower than that in leaf and that mitochondria shsp in leaf was cold-inducible. In this paper, the molecular features of the cloned genes, the causes of the uncommon heat response temperatures of sHSP in newer and the cold inducible character of mitochondria shsp gene in leaf were discussed.
文摘Inspite of the large amount of low molecular weight heat shock protein (LMW HSP) present in plant, its function has still not been clearly known. Understanding the distribution and movement of LMW HSP in cells could provide useful information about its biological functions. A 14 kD HSP was purified from the microsome isolated from the bean of a highly thermotolerant plant Phaseolus vulgaris. Antiserum against this protein was preparaed. The localization of the protein in the cell was analysed by means of electromicroscopic immunogold labelling method. The images of electromicrograph showed that 14 kD HSP mainly existed in both cytoplasm and endoplasmic reticulum and that no labelled gold was found in tonoplasma, mitochondria or cell wall.
