Cotton is the most important economic crop, and its fiber is the major raw material used for textile industry. Cotton fiber with its good properties such as thermal insulation, hygroscopicity, and air_permeability, ca...Cotton is the most important economic crop, and its fiber is the major raw material used for textile industry. Cotton fiber with its good properties such as thermal insulation, hygroscopicity, and air_permeability, can not be replaced by other fibers. With rapid development of textile technology and the increasing requirement of consumers for higher quality of textile products, it is urgent to improve cotton fiber quality, especially its strength and color. There is genetically a negative correlation between the yield and the quality of cotton, therefore molecular engineering_breeding could be an access to concordant improvement of both yield and quality of cotton fiber. This paper reviewed the progress of analyzing cotton fiber_related genes and the present status of improving cotton fiber quality by genetic engineering. Some breakthroughs are expected in separating fiber development_related genes with high throughput techniques and in exploring the novel technology to manipulate transgenic cottons.展开更多
Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of ini...Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process.展开更多
文摘Cotton is the most important economic crop, and its fiber is the major raw material used for textile industry. Cotton fiber with its good properties such as thermal insulation, hygroscopicity, and air_permeability, can not be replaced by other fibers. With rapid development of textile technology and the increasing requirement of consumers for higher quality of textile products, it is urgent to improve cotton fiber quality, especially its strength and color. There is genetically a negative correlation between the yield and the quality of cotton, therefore molecular engineering_breeding could be an access to concordant improvement of both yield and quality of cotton fiber. This paper reviewed the progress of analyzing cotton fiber_related genes and the present status of improving cotton fiber quality by genetic engineering. Some breakthroughs are expected in separating fiber development_related genes with high throughput techniques and in exploring the novel technology to manipulate transgenic cottons.
文摘Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process.
