The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was e...The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.展开更多
语言学家乔姆斯基认为,任何一句话都具有两个结构层次(levels of speech),其一为“表层结构”(Surface Structure),即:One Stracture is that of the sentence as it is pronounced or writing.其二为“深层结构”(deep structure),即:S...语言学家乔姆斯基认为,任何一句话都具有两个结构层次(levels of speech),其一为“表层结构”(Surface Structure),即:One Stracture is that of the sentence as it is pronounced or writing.其二为“深层结构”(deep structure),即:Structure contains all the units and relationships that are necessary for interpreting the meaning of sentence.一个跃子纸上的句子,其表层结构中的表层意义(surface meaning),一目了然,而句子的真正内涵,或深层意义(deep meaning),则需要从不同的侧重点,不同的语境进行深入地考究。人们看到“Woman”一词,反映到头脑里不仅是“妇女”或“结过婚的成年女子”,还会从妇女的社会地位,特点,本质中得出更深层的理解:’frail(脆弱的),“prone to tears”(爱哭),“emotional”(易动感情的),“gentle”展开更多
文摘The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.
文摘语言学家乔姆斯基认为,任何一句话都具有两个结构层次(levels of speech),其一为“表层结构”(Surface Structure),即:One Stracture is that of the sentence as it is pronounced or writing.其二为“深层结构”(deep structure),即:Structure contains all the units and relationships that are necessary for interpreting the meaning of sentence.一个跃子纸上的句子,其表层结构中的表层意义(surface meaning),一目了然,而句子的真正内涵,或深层意义(deep meaning),则需要从不同的侧重点,不同的语境进行深入地考究。人们看到“Woman”一词,反映到头脑里不仅是“妇女”或“结过婚的成年女子”,还会从妇女的社会地位,特点,本质中得出更深层的理解:’frail(脆弱的),“prone to tears”(爱哭),“emotional”(易动感情的),“gentle”
