[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a g...[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a glyphosate manufacture.The fungal strain A-F02 was identified according to morphological characteristics and internal transcribed spacer(ITS)region of nuclear ribosomal DNA sequence analysis.The glyphosate-biodegraded characteristics of strain A-F02 and the influencing factors were studied.[Result] The fungal strain A-F02 was identified as Aspergillus oryzae sp..The glyphosate-biodegraded rate was 86.82% in the mineral salt medium with 1 000 mg/L of glyphosate as the sole source of carbon,after being incubated at 30 ℃ and 150 rpm for 7 d.The biodegradation rates and biomass of the A-F02 were the highest under the culture conditions with glucose(0.5%,w/v),pH 7.5,30 ℃ and glyphosate(1 500 mg/L).[Conclusion] The research provided the experimental basis for glyphosate-biodegraded enzyme purification.展开更多
首次研究了一种添加剂——过氧化苯甲酰(BPO)对高密度聚乙烯在超临界水中降解行为的影响。对使用添加剂和未使用添加剂的实验产物采取多种方式检测对比,得到结论:在降解反应的前30 m in内,聚乙烯降解效率最高;温度是影响降解反应的重要...首次研究了一种添加剂——过氧化苯甲酰(BPO)对高密度聚乙烯在超临界水中降解行为的影响。对使用添加剂和未使用添加剂的实验产物采取多种方式检测对比,得到结论:在降解反应的前30 m in内,聚乙烯降解效率最高;温度是影响降解反应的重要因素;在反应温度较低和反应时间较短的情况下,添加BPO可以有效促进聚乙烯的降解,达到与未添加BPO实验中高温和长时间反应相比拟的效果。同时本文讨论了聚乙烯在超临界水中降解反应机理,对BPO在反应中的促进作用也进行了分析。展开更多
文摘[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a glyphosate manufacture.The fungal strain A-F02 was identified according to morphological characteristics and internal transcribed spacer(ITS)region of nuclear ribosomal DNA sequence analysis.The glyphosate-biodegraded characteristics of strain A-F02 and the influencing factors were studied.[Result] The fungal strain A-F02 was identified as Aspergillus oryzae sp..The glyphosate-biodegraded rate was 86.82% in the mineral salt medium with 1 000 mg/L of glyphosate as the sole source of carbon,after being incubated at 30 ℃ and 150 rpm for 7 d.The biodegradation rates and biomass of the A-F02 were the highest under the culture conditions with glucose(0.5%,w/v),pH 7.5,30 ℃ and glyphosate(1 500 mg/L).[Conclusion] The research provided the experimental basis for glyphosate-biodegraded enzyme purification.
文摘首次研究了一种添加剂——过氧化苯甲酰(BPO)对高密度聚乙烯在超临界水中降解行为的影响。对使用添加剂和未使用添加剂的实验产物采取多种方式检测对比,得到结论:在降解反应的前30 m in内,聚乙烯降解效率最高;温度是影响降解反应的重要因素;在反应温度较低和反应时间较短的情况下,添加BPO可以有效促进聚乙烯的降解,达到与未添加BPO实验中高温和长时间反应相比拟的效果。同时本文讨论了聚乙烯在超临界水中降解反应机理,对BPO在反应中的促进作用也进行了分析。