Here is the abstract of the final report for the project "Improvement of Anesthesia and Analgesia of Acupuncture Combined with Drugs: Clinical Observation and Experimental Research", one of the national key ...Here is the abstract of the final report for the project "Improvement of Anesthesia and Analgesia of Acupuncture Combined with Drugs: Clinical Observation and Experimental Research", one of the national key projects in the ninth five year plan.The results of clinical and experimental studies from Beijing, Chengdu and Shanghai showed that acupuncture combined with drugs is the very useful method during clinical anesthesia (no matter epidural anesthesia or general anesthesia) and analgesia (such as pain during labor, post operative pain and sciatic neuralgia). Acupuncture can not only produce analgesia, but also protect the physiological functions of many organs such as brain, heart, kidney and throat. By the way, some new progress about the experimental research of the mechanism of acupuncture analgesia has been carried out, such as the relationship between the new discovered neuro modulators (endomorphin, orphanin FQ) and acupuncture analgesia.展开更多
Orphanin FQ(OFQ, also named "nociceptin"), a new member of the opioid peptides f amily, was discovered in 1995. The relationship between OFQ and pain modulation or acupuncture analgesia was investigated in o...Orphanin FQ(OFQ, also named "nociceptin"), a new member of the opioid peptides f amily, was discovered in 1995. The relationship between OFQ and pain modulation or acupuncture analgesia was investigated in our research group. It was found t hat icv injection of OFQ could attenuate opioid analgesia and acupuncture analge sia, but OFQ in spinal cord produced significant analgesia, and had synergic eff ect with endomorphin induced analgesia. OFQ in the brain could inhibit the activ ities of opioid system, including release of the peptides and expression of the peptides and their receptors. But in the spinal cord OFQ would increase the rele ase of endomorphin. Electroacupuncture could increase the activities of OFQ syst em in the brain, which might be one of mechanisms of incomplete analgesia by acu puncture. So the effect of acupuncture analgesia would be enhanced through the i nhibition of OFQ system in the brain. In the present studies, it was also found that some OFQ like immunoreactive neurons in the brain and the spinal cord coul d express OFQ receptor (self receptor), and NC NH 2, one kind of new antagoni st of OFQ receptor, might be indicated an agonist. According to these above results, it is suggested that OFQ is a new importan t factor involving acupuncture analgesia.展开更多
The present study was systematically carried out to observe the effects of elect roacupuncture on neuropathic pain and to investigate the preliminary molecular m echanisms of electroacupuncture analgesia in neuropathi...The present study was systematically carried out to observe the effects of elect roacupuncture on neuropathic pain and to investigate the preliminary molecular m echanisms of electroacupuncture analgesia in neuropathic pain by using behaviora l and in situ hybridization methods. The results were as follows: In CCI (chronic constriction injury) rats, hyperalgesia score is used as the sig n of neuropathic pain. Pain threshold after immediate electroacupuncture(EA) and the next day after repeated electroacupuncture was observed to investigate effe ct of electroacupuncture on hyperalgesia score of neuropathic pain. It showed th at on day 7 after CCI operation, controlateral EA of "Huantiao"(GB 30) and "Y anglingquan"(GB 34) acupoints (4 and 20 Hz alternately, 2.5 sec and 5 sec respe ctively, ≤1 mA, 30 min) immediately and significantly increased hyperalge sia score in neuropathic pain rats. When EA was given (the same as the former) o n day 9,11,13,16,18,20,23,25,27 after CCI operation, hyperalgesia score was obse rved the following day. The more EA was given, the higher the hyperalgesia score became, which showed an accumulative effect. It is thus suggested that EA ca n produce immediate analgesia in neuropathic pain rats, and have ac cumulative effects. Based on these, we will continue to investigate the expression of POMC mRNA in arcuate hypothalamic nucleus and the expression of ppOFQ mRNA in dorsal horn in neuropathic pain rats after EA treatment. Results showed that the expression of POMC mRNA in arcuate hypothalamic nucleus was increased significantly on day 7 a fter CCI operation, and had downward trend later. On day 7 after operation, the POMC mRNA expression was enhanced immediately and markedly 8 hours after one E A treatment. After repeated EA stimulation in neuropathic pain rats, the POMC mRN A expression was continually and obviously increased. These results suggested th at the continual increase of brain POMC mRNA expression might be one of the impo rtant factors involving EA analgesia in ne uropathic pain. Results also showed that the expression of ppOFQ mRNA in ipsilateral dorsal horn was decreased significantly on day 7 after CCI operation, and recovered a littl e later on. On day 7 after CCI operation, the ppOFQ mRNA expression in ipsilater al dorsal horn was immediately and markedly enhanced 8 hours after one EA treatm ent. After repeated EA stimulation in neuropathic pain rats, the ppOFQ mRNA expr ession was continually and obviously increased to normal. These results suggeste d that the recovery of spinal ppOFQ mRNA might be another important mechanism of EA analgesia in neuropathic pain.展开更多
目的:观察电针对神经病理性疼痛大鼠痛行为学及脊髓细胞因子信号转导抑制蛋白3(SOCS3)表达的影响。方法:SD大鼠随机分为正常组、假手术组、模型组、电针组、非电针组,每组10只。采用坐骨神经结扎压迫建立慢性限制性损伤(CCI)神经痛模型...目的:观察电针对神经病理性疼痛大鼠痛行为学及脊髓细胞因子信号转导抑制蛋白3(SOCS3)表达的影响。方法:SD大鼠随机分为正常组、假手术组、模型组、电针组、非电针组,每组10只。采用坐骨神经结扎压迫建立慢性限制性损伤(CCI)神经痛模型,电针组选取双侧"阳陵泉"、"环跳"穴进行电针治疗,观察大鼠痛行为学和脊髓SOCS3、IL-6基因表达的变化,检测脊髓SOCS3神经细胞定位情况。结果:与模型组相比,电针可显著提高CCI大鼠机械痛及热痛阈值(P<0.01);与正常组相比,模型组大鼠脊髓SOCS3、IL-6 m RNA表达显著上调(P<0.01),而电针可显著降低IL-6m RNA表达,并进一步上调脊髓SOCS3表达水平(P<0.01)。在CCI大鼠中,SOCS3主要表达于脊髓星形胶质细胞和小胶质细胞中。结论:电针可能通过下调IL-6基因表达,并上调SOCS3表达,减轻神经痛大鼠痛敏反应。展开更多
文摘Here is the abstract of the final report for the project "Improvement of Anesthesia and Analgesia of Acupuncture Combined with Drugs: Clinical Observation and Experimental Research", one of the national key projects in the ninth five year plan.The results of clinical and experimental studies from Beijing, Chengdu and Shanghai showed that acupuncture combined with drugs is the very useful method during clinical anesthesia (no matter epidural anesthesia or general anesthesia) and analgesia (such as pain during labor, post operative pain and sciatic neuralgia). Acupuncture can not only produce analgesia, but also protect the physiological functions of many organs such as brain, heart, kidney and throat. By the way, some new progress about the experimental research of the mechanism of acupuncture analgesia has been carried out, such as the relationship between the new discovered neuro modulators (endomorphin, orphanin FQ) and acupuncture analgesia.
文摘Orphanin FQ(OFQ, also named "nociceptin"), a new member of the opioid peptides f amily, was discovered in 1995. The relationship between OFQ and pain modulation or acupuncture analgesia was investigated in our research group. It was found t hat icv injection of OFQ could attenuate opioid analgesia and acupuncture analge sia, but OFQ in spinal cord produced significant analgesia, and had synergic eff ect with endomorphin induced analgesia. OFQ in the brain could inhibit the activ ities of opioid system, including release of the peptides and expression of the peptides and their receptors. But in the spinal cord OFQ would increase the rele ase of endomorphin. Electroacupuncture could increase the activities of OFQ syst em in the brain, which might be one of mechanisms of incomplete analgesia by acu puncture. So the effect of acupuncture analgesia would be enhanced through the i nhibition of OFQ system in the brain. In the present studies, it was also found that some OFQ like immunoreactive neurons in the brain and the spinal cord coul d express OFQ receptor (self receptor), and NC NH 2, one kind of new antagoni st of OFQ receptor, might be indicated an agonist. According to these above results, it is suggested that OFQ is a new importan t factor involving acupuncture analgesia.
文摘The present study was systematically carried out to observe the effects of elect roacupuncture on neuropathic pain and to investigate the preliminary molecular m echanisms of electroacupuncture analgesia in neuropathic pain by using behaviora l and in situ hybridization methods. The results were as follows: In CCI (chronic constriction injury) rats, hyperalgesia score is used as the sig n of neuropathic pain. Pain threshold after immediate electroacupuncture(EA) and the next day after repeated electroacupuncture was observed to investigate effe ct of electroacupuncture on hyperalgesia score of neuropathic pain. It showed th at on day 7 after CCI operation, controlateral EA of "Huantiao"(GB 30) and "Y anglingquan"(GB 34) acupoints (4 and 20 Hz alternately, 2.5 sec and 5 sec respe ctively, ≤1 mA, 30 min) immediately and significantly increased hyperalge sia score in neuropathic pain rats. When EA was given (the same as the former) o n day 9,11,13,16,18,20,23,25,27 after CCI operation, hyperalgesia score was obse rved the following day. The more EA was given, the higher the hyperalgesia score became, which showed an accumulative effect. It is thus suggested that EA ca n produce immediate analgesia in neuropathic pain rats, and have ac cumulative effects. Based on these, we will continue to investigate the expression of POMC mRNA in arcuate hypothalamic nucleus and the expression of ppOFQ mRNA in dorsal horn in neuropathic pain rats after EA treatment. Results showed that the expression of POMC mRNA in arcuate hypothalamic nucleus was increased significantly on day 7 a fter CCI operation, and had downward trend later. On day 7 after operation, the POMC mRNA expression was enhanced immediately and markedly 8 hours after one E A treatment. After repeated EA stimulation in neuropathic pain rats, the POMC mRN A expression was continually and obviously increased. These results suggested th at the continual increase of brain POMC mRNA expression might be one of the impo rtant factors involving EA analgesia in ne uropathic pain. Results also showed that the expression of ppOFQ mRNA in ipsilateral dorsal horn was decreased significantly on day 7 after CCI operation, and recovered a littl e later on. On day 7 after CCI operation, the ppOFQ mRNA expression in ipsilater al dorsal horn was immediately and markedly enhanced 8 hours after one EA treatm ent. After repeated EA stimulation in neuropathic pain rats, the ppOFQ mRNA expr ession was continually and obviously increased to normal. These results suggeste d that the recovery of spinal ppOFQ mRNA might be another important mechanism of EA analgesia in neuropathic pain.
文摘目的:观察电针对神经病理性疼痛大鼠痛行为学及脊髓细胞因子信号转导抑制蛋白3(SOCS3)表达的影响。方法:SD大鼠随机分为正常组、假手术组、模型组、电针组、非电针组,每组10只。采用坐骨神经结扎压迫建立慢性限制性损伤(CCI)神经痛模型,电针组选取双侧"阳陵泉"、"环跳"穴进行电针治疗,观察大鼠痛行为学和脊髓SOCS3、IL-6基因表达的变化,检测脊髓SOCS3神经细胞定位情况。结果:与模型组相比,电针可显著提高CCI大鼠机械痛及热痛阈值(P<0.01);与正常组相比,模型组大鼠脊髓SOCS3、IL-6 m RNA表达显著上调(P<0.01),而电针可显著降低IL-6m RNA表达,并进一步上调脊髓SOCS3表达水平(P<0.01)。在CCI大鼠中,SOCS3主要表达于脊髓星形胶质细胞和小胶质细胞中。结论:电针可能通过下调IL-6基因表达,并上调SOCS3表达,减轻神经痛大鼠痛敏反应。
