Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were ana...Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.展开更多
protoplasts having only 24～28 chromosomes and callus derived Haynaldia villosa potoplasts with 11 14 chromosomes was carried out by a PEG method. A high frequency of putative hybrid calli and plants were regenerated ...protoplasts having only 24～28 chromosomes and callus derived Haynaldia villosa potoplasts with 11 14 chromosomes was carried out by a PEG method. A high frequency of putative hybrid calli and plants were regenerated from fusion products. Their hybrid nature was identified by cytological, biochemical and 5S rDNA spacer sequence analysis. The results showed that all the analyzed regenerated calli and plants were hybrids. GISH (genomic in situ hybridization) analysis proved the existence of the chromosomes of both donors and the occurrence of translocation between the chromosomes of the two species in the hybrid clones. The analysis of the cytoplasmic DNA using mitochondrion and chloroplast specific probes revealed that some degree of recombination between the organellar genome of the two species occurred. The gross morphology of hybrid plants resembled that of two donors. One hybrid was fertile and gave rise to seed, which resembled that of Haynaldia villosa in morphology. In conclusion, we generated fertile somatic hybrid by intergeneric somatic hybridization. The co existence of both species’chromosomes in the hybrid clones in relation to their regeneration capacity and the production of fertile hybrid plants will be discussed in the speech.展开更多
文摘Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.
文摘protoplasts having only 24～28 chromosomes and callus derived Haynaldia villosa potoplasts with 11 14 chromosomes was carried out by a PEG method. A high frequency of putative hybrid calli and plants were regenerated from fusion products. Their hybrid nature was identified by cytological, biochemical and 5S rDNA spacer sequence analysis. The results showed that all the analyzed regenerated calli and plants were hybrids. GISH (genomic in situ hybridization) analysis proved the existence of the chromosomes of both donors and the occurrence of translocation between the chromosomes of the two species in the hybrid clones. The analysis of the cytoplasmic DNA using mitochondrion and chloroplast specific probes revealed that some degree of recombination between the organellar genome of the two species occurred. The gross morphology of hybrid plants resembled that of two donors. One hybrid was fertile and gave rise to seed, which resembled that of Haynaldia villosa in morphology. In conclusion, we generated fertile somatic hybrid by intergeneric somatic hybridization. The co existence of both species’chromosomes in the hybrid clones in relation to their regeneration capacity and the production of fertile hybrid plants will be discussed in the speech.
