目的:基于网络药理学分析艾迪注射液抗NSCLC关键靶点和信号通路探讨其潜在的作用机制。方法:应用TCMSP数据库及TCMID数据库分别检索艾迪注射液中四味药物斑蝥、黄芪、人参、刺五加的化学成分及其对应的靶点,检索GEO数据库中NSCLC的潜在...目的:基于网络药理学分析艾迪注射液抗NSCLC关键靶点和信号通路探讨其潜在的作用机制。方法:应用TCMSP数据库及TCMID数据库分别检索艾迪注射液中四味药物斑蝥、黄芪、人参、刺五加的化学成分及其对应的靶点,检索GEO数据库中NSCLC的潜在靶点,利用Perl将药物成分、成分靶点、疾病靶点等文件融合,通过PPI网络的拓扑结构分析能够发现其中的关键蛋白及其相互关系,利用clusterProfiler程辑包[100]进行GO及KEGG富集分析。结果:通过网络药理学分析,获取艾迪注射液的化学成分有51个,将化学成分靶点与疾病靶点取交集后得到81个艾迪治疗NSCLC的靶点,证明艾迪的抗肿瘤作用是通过多成分–多靶点的途径实现的。成分–靶点网路图的拓扑分析显示槲皮素、山柰酚、7-O-甲基异微凸剑叶莎醇、异鼠李素、豆甾醇等度值较高,可能为艾迪有效成分中的关键药效物质,靶点方面PTGS2、ADRB2、ALOX5等度值较高,可能作为艾迪抗NSCLC的重要靶点。结论:本研究初步探明了艾迪注射液抗NSCLC的化学成分、靶点通路及相互作用关系。艾迪中抗NSCLC的关键成分为槲皮素、山柰酚、异鼠李素、豆甾醇等,主要是通过抗氧化、抑制EMT、调节金属离子及类固醇激素应答、促进肿瘤细胞凋亡等方式来发挥抗癌作用。Objective: To analyze the key targets and signaling pathways of Aidi injection against NSCLC based on network pharmacology to explore its potential mechanism of action. Methods: The TCMSP database and TCMID database were used to search the chemical constituents and their corresponding targets of the four drugs Zanthoxylum, Astragalus, Ginseng and Acanthopanax in Aidi injection, and to search the potential targets of NSCLC in the GEO database, and the files of drug constituents, constituent targets and disease targets were fused by using Perl, and the key protein and their interrelationships could be found through the topology analysis of the PPI network. Key proteins and their interrelationships were identified by topological analysis of the PPI network, and GO and KEGG enrichment analyses were carried out using the cluster Profiler program package [100]. Results: Through the network pharmacology analysis, 51 chemical components of Aidi injection were obtained, and 81 targets of Aidi for NSCLC were obtained after taking the intersection of chemical component targets and disease targets, which proved that the antitumor effect of Aidi was realized through the multi-component-multi-target pathway. The topological analysis of the component-target network diagram showed that quercetin, kaempferol, 7-O-methylisocamptothecin, isorhamnetin, and stigmasterol had higher degree values, which might be the key pharmacodynamic substances in the active ingredients of Aidi, and the targets of PTGS2, ADRB2, and ALOX5 had higher degree values, which might be the important targets of Aidi for the anti-NSCLC. Conclusion: This study preliminarily investigated the chemical composition, target pathway and interaction relationship of anti-NSCLC in Aidi injection. The key anti-NSCLC components in Aidi are quercetin, kaempferol, isorhamnetin, and stigmasterol, which mainly exert their anticancer effects through antioxidant, EMT inhibition, modulation of metal ions and steroid hormone response, and promotion of tumor cell apoptosis.展开更多
目的:探讨艾灸对大鼠骨骼肌力量耐力的调节作用。方法:将30只雄性SD大鼠随机分为对照组、模型组、艾灸组3组。对照组、模型组不干预,艾灸组采用回旋灸大椎穴、后三里穴等20min;对照组直接麻醉并进行肌肉力量检测,模型组直接麻醉造模并...目的:探讨艾灸对大鼠骨骼肌力量耐力的调节作用。方法:将30只雄性SD大鼠随机分为对照组、模型组、艾灸组3组。对照组、模型组不干预,艾灸组采用回旋灸大椎穴、后三里穴等20min;对照组直接麻醉并进行肌肉力量检测,模型组直接麻醉造模并进行肌肉力量耐力检测,艾灸组回旋灸后麻醉造模并进行肌肉力量耐力检测。造模采用电刺激坐骨神经诱导腓肠肌疲劳实验,用PowerLab/8SP生理记录仪记录腓肠肌收缩振幅随时间变化的疲劳曲线(F-T曲线),分别选取疲劳实验中第1、10、20、30、40、50次刺激,计算相对应的力量耐力平均值(force endurance mean,FEm),力量耐力最大值(maximal force endurance,FEmax);对照组采用造模方法中的电刺激参数间隔刺激6次,并计算出相对应的FEm和FEmax,与模型组和艾灸组比较,分析力量耐力变化。结果:(1)模型组20FEm与对照组相比显著下降(P<0.05),模型组30FEm、40FEm、50FEm与对照组相比显著下降(P<0.01);艾灸组仅40FEm与对照组相比有显著性差异(P<0.05)。(2)模型组20FEmax、30FEmax、40FEmax、50FEmax与对照组相比显著下降(P<0.05,P<0.01),艾灸组40FEmax、50FEmax与对照组相比显著下降(P<0.05,P<0.01)。结论:艾灸对大鼠骨骼肌力量耐力有调节效应,可延缓力量耐力下降点过早出现,相对增强大鼠骨骼肌力量耐力,延缓运动性疲劳的发生。展开更多
文摘目的:基于网络药理学分析艾迪注射液抗NSCLC关键靶点和信号通路探讨其潜在的作用机制。方法:应用TCMSP数据库及TCMID数据库分别检索艾迪注射液中四味药物斑蝥、黄芪、人参、刺五加的化学成分及其对应的靶点,检索GEO数据库中NSCLC的潜在靶点,利用Perl将药物成分、成分靶点、疾病靶点等文件融合,通过PPI网络的拓扑结构分析能够发现其中的关键蛋白及其相互关系,利用clusterProfiler程辑包[100]进行GO及KEGG富集分析。结果:通过网络药理学分析,获取艾迪注射液的化学成分有51个,将化学成分靶点与疾病靶点取交集后得到81个艾迪治疗NSCLC的靶点,证明艾迪的抗肿瘤作用是通过多成分–多靶点的途径实现的。成分–靶点网路图的拓扑分析显示槲皮素、山柰酚、7-O-甲基异微凸剑叶莎醇、异鼠李素、豆甾醇等度值较高,可能为艾迪有效成分中的关键药效物质,靶点方面PTGS2、ADRB2、ALOX5等度值较高,可能作为艾迪抗NSCLC的重要靶点。结论:本研究初步探明了艾迪注射液抗NSCLC的化学成分、靶点通路及相互作用关系。艾迪中抗NSCLC的关键成分为槲皮素、山柰酚、异鼠李素、豆甾醇等,主要是通过抗氧化、抑制EMT、调节金属离子及类固醇激素应答、促进肿瘤细胞凋亡等方式来发挥抗癌作用。Objective: To analyze the key targets and signaling pathways of Aidi injection against NSCLC based on network pharmacology to explore its potential mechanism of action. Methods: The TCMSP database and TCMID database were used to search the chemical constituents and their corresponding targets of the four drugs Zanthoxylum, Astragalus, Ginseng and Acanthopanax in Aidi injection, and to search the potential targets of NSCLC in the GEO database, and the files of drug constituents, constituent targets and disease targets were fused by using Perl, and the key protein and their interrelationships could be found through the topology analysis of the PPI network. Key proteins and their interrelationships were identified by topological analysis of the PPI network, and GO and KEGG enrichment analyses were carried out using the cluster Profiler program package [100]. Results: Through the network pharmacology analysis, 51 chemical components of Aidi injection were obtained, and 81 targets of Aidi for NSCLC were obtained after taking the intersection of chemical component targets and disease targets, which proved that the antitumor effect of Aidi was realized through the multi-component-multi-target pathway. The topological analysis of the component-target network diagram showed that quercetin, kaempferol, 7-O-methylisocamptothecin, isorhamnetin, and stigmasterol had higher degree values, which might be the key pharmacodynamic substances in the active ingredients of Aidi, and the targets of PTGS2, ADRB2, and ALOX5 had higher degree values, which might be the important targets of Aidi for the anti-NSCLC. Conclusion: This study preliminarily investigated the chemical composition, target pathway and interaction relationship of anti-NSCLC in Aidi injection. The key anti-NSCLC components in Aidi are quercetin, kaempferol, isorhamnetin, and stigmasterol, which mainly exert their anticancer effects through antioxidant, EMT inhibition, modulation of metal ions and steroid hormone response, and promotion of tumor cell apoptosis.
文摘目的:探讨艾灸对大鼠骨骼肌力量耐力的调节作用。方法:将30只雄性SD大鼠随机分为对照组、模型组、艾灸组3组。对照组、模型组不干预,艾灸组采用回旋灸大椎穴、后三里穴等20min;对照组直接麻醉并进行肌肉力量检测,模型组直接麻醉造模并进行肌肉力量耐力检测,艾灸组回旋灸后麻醉造模并进行肌肉力量耐力检测。造模采用电刺激坐骨神经诱导腓肠肌疲劳实验,用PowerLab/8SP生理记录仪记录腓肠肌收缩振幅随时间变化的疲劳曲线(F-T曲线),分别选取疲劳实验中第1、10、20、30、40、50次刺激,计算相对应的力量耐力平均值(force endurance mean,FEm),力量耐力最大值(maximal force endurance,FEmax);对照组采用造模方法中的电刺激参数间隔刺激6次,并计算出相对应的FEm和FEmax,与模型组和艾灸组比较,分析力量耐力变化。结果:(1)模型组20FEm与对照组相比显著下降(P<0.05),模型组30FEm、40FEm、50FEm与对照组相比显著下降(P<0.01);艾灸组仅40FEm与对照组相比有显著性差异(P<0.05)。(2)模型组20FEmax、30FEmax、40FEmax、50FEmax与对照组相比显著下降(P<0.05,P<0.01),艾灸组40FEmax、50FEmax与对照组相比显著下降(P<0.05,P<0.01)。结论:艾灸对大鼠骨骼肌力量耐力有调节效应,可延缓力量耐力下降点过早出现,相对增强大鼠骨骼肌力量耐力,延缓运动性疲劳的发生。
