The 18 species of bird studied originally are known to belong to muscicapids, robins and sylviids of passerines, but some dis- putations are always present in their classification systems. In this experiment, phylogen...The 18 species of bird studied originally are known to belong to muscicapids, robins and sylviids of passerines, but some dis- putations are always present in their classification systems. In this experiment, phylogenetic relationships of 18 species of passerines were studied using Adenylate Kinase lntron 5 （AKS） sequences and DNA techniques. Through sequences analysis in comparison with each other, phylogenetic tree figures of 18 species of passerines were constructed using Neighbor-Joining （N J） and Maximum-Parsimony （MP） meth- ods . The results showed that sylviids should be listed as an independent family, while robins and flycatchers should be listed into Musci- capidae. Since the phylogenetic relationships between long-tailed tits and old world warblers are closer than that between long-tailed tits and parids, the long-tailed tits should be independent of paridae and be categorized into aegithalidae. Muscicapidae and Paridae are known to be two monophylitic families, but Sylviidae is not a monophyletic group. AK5 sequences had better efficacy in resolving close relationships of interspecies among intrageneric groups.展开更多
对白桦花序进行分期取材,CTAB法提取总RNA,基于SMART(Switching Mechanism At5'end of RNATranscript)策略进行cDNA合成,根据现有EST序列设计基因特异性引物,通过RACE(rapid amplification of cDNAend)手段克隆了一花发育相关基因--...对白桦花序进行分期取材,CTAB法提取总RNA,基于SMART(Switching Mechanism At5'end of RNATranscript)策略进行cDNA合成,根据现有EST序列设计基因特异性引物,通过RACE(rapid amplification of cDNAend)手段克隆了一花发育相关基因---BplAGL,使用TreeTOP工具对其系统发生进行了评价。采用外标实时定量PCR分析其花期时序表达模式,认为BplAGL可能属于D功能基因。展开更多
文摘The 18 species of bird studied originally are known to belong to muscicapids, robins and sylviids of passerines, but some dis- putations are always present in their classification systems. In this experiment, phylogenetic relationships of 18 species of passerines were studied using Adenylate Kinase lntron 5 （AKS） sequences and DNA techniques. Through sequences analysis in comparison with each other, phylogenetic tree figures of 18 species of passerines were constructed using Neighbor-Joining （N J） and Maximum-Parsimony （MP） meth- ods . The results showed that sylviids should be listed as an independent family, while robins and flycatchers should be listed into Musci- capidae. Since the phylogenetic relationships between long-tailed tits and old world warblers are closer than that between long-tailed tits and parids, the long-tailed tits should be independent of paridae and be categorized into aegithalidae. Muscicapidae and Paridae are known to be two monophylitic families, but Sylviidae is not a monophyletic group. AK5 sequences had better efficacy in resolving close relationships of interspecies among intrageneric groups.
文摘对白桦花序进行分期取材,CTAB法提取总RNA,基于SMART(Switching Mechanism At5'end of RNATranscript)策略进行cDNA合成,根据现有EST序列设计基因特异性引物,通过RACE(rapid amplification of cDNAend)手段克隆了一花发育相关基因---BplAGL,使用TreeTOP工具对其系统发生进行了评价。采用外标实时定量PCR分析其花期时序表达模式,认为BplAGL可能属于D功能基因。
